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We have shown that continuous maternal exposure to the complex mixture of environmental chemicals (ECs) found in human biosolids (sewage sludge), disrupts mRNA expression of genes crucial for development and long-term regulation of hypothalamic-pituitary gonadal (HPG) function in sheep. The present study investigated whether exposure to ECs only during preconceptional period or only during pregnancy perturbed key regulatory genes within the hypothalamus and pituitary gland and whether these effects were different from chronic (life-long) exposure to biosolid ECs. The findings demonstrate that the timing and duration of maternal EC exposure influences the subsequent effects on the foetal neuroendocrine system in a sex-specific manner. Maternal exposure prior to conception, or during pregnancy only, altered the expression of key foetal neuroendocrine regulatory systems such as gonadotrophin-releasing hormone and kisspeptin to a greater extent than when maternal exposure was 'life-long'. Furthermore, hypothalamic gene expression was affected to a greater extent in males than in females and, following EC exposure, male foetuses expressed more 'female-like' mRNA levels for some key neuroendocrine genes. This is the first study to show that 'real-life' maternal exposure to low levels of a complex cocktail of chemicals prior to conception can subsequently affect the developing foetal neuroendocrine system. These findings demonstrate that the developing neuroendocrine system is sensitive to EC mixtures in a sex-dimorphic manner likely to predispose to reproductive dysfunction in later life. [ABSTRACT FROM AUTHOR]

Day One Skills (DOS) were introduced by the Royal College of Veterinary Surgeons (RCVS) in 2006 as a guideline for minimum skills required by a veterinary graduate. However, the RCVS anaesthesia DOS are broad and do not specify differences in skills required for different species. The aims of this study were: (1) to determine which anaesthesia skills were considered essential for day one practice by UK-based veterinary practitioners (GPs) and anaesthetists; and (2) to explore current opinions on veterinary undergraduate anaesthesia training. Questionnaires for veterinary GPs (QGPs) and veterinary anaesthetists (QVAs) were developed which asked general information on expectations of anaesthesia skills as well as specific expectations for the common veterinary species. Fifty-five UK-based members of the Association of Veterinary Anaesthetists responded, with a random sample of veterinary practices stratified by UK county generating 234 responses and a convenience sample targeted at more specialist veterinary specialities in the UK generating 161 responses. There was close overall agreement between the two groups of GPs and anaesthetists on essential anaesthesia DOS. However, expectations varied with species—greatest in cats and dogs, lowest in exotics. Many respondents commented that new veterinary graduates lack practical skills and should not be expected to be omnicompetent across all species. In conclusion, anaesthesia undergraduate training should prioritise essential practical DOS. [ABSTRACT FROM AUTHOR]

There is ongoing debate in the profession as to whether veterinary students should focus on one (or a small number of) species during their undergraduate training (ie, track). This paper presents the qualitative data from surveys evaluating UK stakeholder opinion on introducing partial tracking (whereby students continue to qualify able to practise in all species) and full tracking (students qualify in a limited number of species with restricted registration). Surveys were distributed to practitioners, students and university staff; 1061 responses were completed. Thematic framework analysis was conducted on the free-text responses; responses were coded to a hierarchical framework developed inductively from the data. Six major themes were identified: choice, flexibility, competency and knowledge, stakeholder implications, specialisation and 'what is a vet?'. The majority of the themes related to both full and partial tracking, usually being more pronounced in full tracking. The theme 'choice' is particularly important in light of the study's quantitative findings on students' awareness of the profession and their career aspirations (presented in a previous paper); should tracking be implemented, veterinary schools will need to take a proactive role in educating and assisting students while making career choices. [ABSTRACT FROM AUTHOR]

There is on-going debate regarding whether veterinary students should focus on one (or a small number of) species during their undergraduate training (ie, track). The aims of this study were to: evaluate UK stakeholders' opinion on partial tracking (whereby students continue to qualify able to practise in all species) and full tracking (students qualify in a limited number of species necessitating restricted registration); and evaluate students' career aspirations in relation to the UK veterinary profession's employment profile. This paper presents the quantitative results of surveys completed by practitioners, students and university staff. The majority of respondents (69.4 per cent) disagreed or strongly disagreed with full tracking, however, there was widespread support for partial tracking (79.0 per cent agreed or strongly agreed). Students favoured partial tracking more so than practitioners (P<0.001). Univariate analysis of demographic factors did not identify differences in opinion regarding tracking within stakeholder groups. Students' knowledge of the UK veterinary employment profile appeared accurate. However, their career aspiration changed with year of the course, and only final year students' intentions were aligned with the profession's current profile. Qualitative data from these surveys are presented in a second paper and include the advantages, disadvantages and implications of partial and full tracking. [ABSTRACT FROM AUTHOR]

Interviews form part of the selection process in many veterinary programmes worldwide. This study aimed to evaluate the influence of the interview on veterinary selection decisions. An admissions cycle of interviews of 181 shortlisted school-leaving applicants to veterinary medicine was investigated. Selection panel pairs (from cohorts of 5 chairs and 11 cointerviewers) independently evaluated applicants before interview on the basis of their written application. Applicants were then interviewed and selectors repeated their evaluations, both independently and after joint discussion. Results of the preinterview and postinterview evaluations of the applicants were analysed statistically. There was slight to moderate agreement between the chair and cointerviewer on selection decisions/rankings taken before interview, but substantial agreement after interview. Agreement between postinterview decisions/rankings and consensus decisions/rankings postinterview was extremely high. Applicant attributes that had the most influence on selection decisions were: 'communication skills', 'overall knowledge gained from work experience' and 'ability to think on their feet'. There was significantly more agreement possible between interviewers with regard to selection decisions based on interview assessment compared with preinterview assessment of written applications. This study suggests that interviews may be a useful and important aid in decision making for selecting the candidates perceived as most suitable for this veterinary programme. [ABSTRACT FROM AUTHOR]

Exposure to ubiquitous, environmental chemicals (ECs) has been hypothesized as a cause for declining male reproductive health. Understanding the long-term effects of EC exposure on reproductive health in humans requires animal models and exposure to 'real life', environmentally relevant, mixtures during development, a life stage of particular sensitivity to ECs. The aim of this study was to evaluate the effects of in utero and post-natal exposure to environmentally relevant levels of ECs, via sewage sludge application to pasture, on the adult male sheep testis. Hormones, liver concentrations of candidate ECs and Sertoli and germ cell numbers in testes of adult rams that were exposed to ECs in sewage sludge in utero, and until weaning via maternal exposure, and post-weaning via grazing pastures fertilized with sewage sludge, were quantified. Evaluated as a single group, exposure to sludge ECs was without significant effect on most parameters. However, a more detailed study revealed that 5 of 12 sludge-exposed rams exhibited major spermatogenic abnormalities. These consisted of major reductions in germ cell numbers per testis or per Sertoli cell and more Sertoli cell-only tubules, when compared with controls, which did not show any such changes. The sludge-related spermatogenic changes in the five affected animals were significantly different from controls ( p < 0.001); Sertoli cell number was unaffected. Hormone profiles and liver candidate EC concentrations were not measurably affected by exposure. We conclude that developmental exposure of male sheep to real-world mixtures of ECs can result in major reduction in germ cell numbers, indicative of impaired sperm production, in a proportion of exposed males. The individual-specific effects are presumed to reflect EC effects on a heterogeneous population in which some individuals may be more susceptible to adverse EC effects. Such effects of EC exposure in humans could have adverse consequences for sperm counts and fertility in some exposed males. [ABSTRACT FROM AUTHOR]

Considerable evidence suggests that veterinary surgeons' mental health is often poorer than comparable populations and that the incidence of suicide is higher among veterinary surgeons than the general public. Veterinary students also appear to suffer from high levels of anxiety and stress, and may possess inadequate coping strategies when faced with adversity. Veterinary students may find it difficult to access central university support systems due to their heavy workload and geographical isolation on some veterinary campuses. A previous study of University of Edinburgh fourth-year veterinary students found that support services located several miles from the main veterinary campus was a barrier to students accessing counselling services. Consequently, a pilot project was initiated, which provided a counselling service at the University of Edinburgh' s rural Easter Bush veterinary campus one afternoon a week during 2010. As part of the evaluation of this service, web-based questionnaires were delivered via e-mail to all veterinary staff and students towards the end of the 12-month pilot period to evaluate perceptions of barriers to student counselling and to investigate student- valued support services. Questionnaire responses were received from 35 per cent of veterinary students and 52 per cent of staff. Stigmatisation of being unable to cope was a potent inhibitor of seeking support within the veterinary environment, but counselling was perceived as valuable by the majority of staff and students. Provision of an on-site counselling service was considered important for increasing ease of access; however, students viewed friends and family as their most important support mechanism. Workload was cited as the main cause of veterinary student stress. The majority of staff and student respondents perceived veterinary students as having an increased need for counselling support compared with other students. [ABSTRACT FROM AUTHOR]

The perceptions of veterinarians and small animal (SA) clients on what attributes constitute 'a good veterinarian' were examined by a questionnaire survey. The respondents were asked to record how important they considered 20 attributes for a veterinary surgeon to have on a five-point scale from 'not at all important' to 'very important'. In addition, they were asked to list which attributes they considered to be the three most important attributes in a veterinary surgeon; finally, they were asked whether there were any additional attributes that they considered to be highly desirable in a veterinary surgeon. In total, 407 SA clients, 243 SA veterinarians and 61 non-SA veterinarians completed the questionnaire. There were significant differences in the proportion of clients who considered an attribute to be 'very important' compared with SA veterinarians for 12 of the 20 attributes (P<0.005). A larger proportion of clients considered 'confidence', 'knowledge about veterinary medicine and surgery', 'cleanliness', 'good at explaining technical terms', 'patience', 'clear about cost of treatment', 'ability to work in a team', 'honesty', 'politeness', 'decisiveness', 'good with animals' and 'good practical skills' to be 'very important' attributes than the SA veterinarians; a larger proportion of SA veterinarians considered 'good communication skills' to be a 'very important' attribute than the clients. [ABSTRACT FROM AUTHOR]

In an effort to increase suicide awareness skills among veterinary undergraduates, a three-hour suicide awareness workshop (safeTALK) was delivered to third-year Royal (Dick) School of Veterinary Studies undergraduates as part of their professional development curriculum. Students were able to opt out of the session by contacting the course organisers. A total of 26 of 151 (17 per cent) third-year students attended the workshop, and 17 completed a feedback questionnaire. The vast majority of the students reported that after completing the workshop they were more likely or much more likely to recognise the signs of a person at risk of suicide, approach a person at risk of suicide, ask a person about suicide, and connect a person at risk of suicide with help. Five veterinary academics attended a two-day Applied Suicide Intervention Skills Training (ASIST) course, and all reported that the course was effective in improving suicide awareness and intervention skills. [ABSTRACT FROM PUBLISHER]

Animals and humans are chronically exposed to endocrine disrupting chemicals (EDCs) that are ubiquitous in the environment. There are strong circumstantial links between environmental EDC exposure and both declining human/wildlife reproductive health and the increasing incidence of reproductive system abnormalities. The verification of such links, however, is difficult and requires animal models exposed to ‘real life’, environmentally relevant concentrations/mixtures of environmental contaminants (ECs), particularly in utero, when sensitivity to EC exposure is high. The present study aimed to determine whether the foetal sheep reproductive neuroendocrine axis, particularly gondotrophin-releasing hormone (GnRH) and galaninergic systems, were affected by maternal exposure to a complex mixture of chemicals, applied to pasture, in the form of sewage sludge. Sewage sludge contains high concentrations of a spectrum of EDCs and other pollutants, relative to environmental concentrations, but is frequently recycled to land as a fertiliser. We found that foetuses exposed to the EDC mixture in utero through their mothers had lower GnRH mRNA expression in the hypothalamus and lower GnRH receptor (GnRHR) and galanin receptor (GALR) mRNA expression in the hypothalamus and pituitary gland. Strikingly, this, treatment had no significant effect on maternal GnRH or GnRHR mRNA expression, although GALR mRNA expression within the maternal hypothalamus and pituitary gland was reduced. The present study clearly demonstrates that the developing foetal neuroendocrine axis is sensitive to real-world mixtures of environmental chemicals. Given the important role of GnRH and GnRHR in the regulation of reproductive function, its known role programming role in utero, and the role of galanin in the regulation of many physiological/neuroendocrine systems, in utero changes in the activity of these systems are likely to have long-term consequences in adulthood and represent a novel pathway through which EC mixtures could perturb normal reproductive function. [ABSTRACT FROM AUTHOR]

The major challenge in veterinary undergraduate admissions is to select those students with most suitability for veterinary training and careers from a large and diverse pool of applicants with very high academic ability. This paper describes a review of the admissions processes of the seven veterinary schools in the UK. There was significant commonality in the entry requirements and the criteria upon which the schools made decisions on candidates. There was some variation in the procedures used by individual schools to select candidates, but common themes existed within these processes. All of the schools evaluated both academic and non-academic factors for individual applicants, and all used interviews in some format as a selection tool after an initial short-listing process. The procedures and approaches to selection processes are compared and discussed. [ABSTRACT FROM AUTHOR]

Contents Pollutants of many chemical classes, derived primarily from anthropogenic activities, are ubiquitous in the environment, persistent, biologically available and can exert adverse effects on the reproductive and other, indirectly related, physiological systems. Food is generally considered to be the major route of animal exposure in vertebrate species but the relative contributions of other routes of exposure such as through lungs, gills or skin are not well studied and may be of importance for certain animal groups, depending on their immediate environment. Animals are particularly sensitive to exposure during developmental stages but the pattern of exposure to chemicals is likely to be different to that of adults. Quantification of the risk posed by the ingestion of pollutants in food is complex and depends on many factors including species, diet composition, duration of exposure to the food, efficiency of pollutant absorption, subsequent metabolism, sensitivity of target organs and stage of development. While the effects of high doses of single chemicals are proven, dietary exposure to pollutants generally involves prolonged, low-level exposure to a large number of compounds, each of which has different chemical characteristics, exerts different biological effects and is present at varying concentrations. Thus, while exposure to pollutants through feed is undoubtedly a significant risk factor for many species and may be the most important one for many terrestrial vertebrates, other routes of exposure may be more important in other groups. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the skin immunopathology of gene gun-delivered plasmid-encoded granulocyte-macrophage colony-stimulating factor (pGM-CSF) and hence explore the possible mechanisms of its adjuvant activity. Using sheep as the experimental model, expressible pGM-CSF was administered to the epidermis and the dermal/epidermal junction and its effects on the skin were assessed by histopathology, immunohistology and quantitative RT-PCR for a range of pro-inflammatory and immune response-polarizing cytokines. Both functional and non-functional plasmids caused an acute inflammatory response with the infiltration of neutrophils and micro-abscess formation; however, the response to pGM-CSF was more severe and was also associated with the accumulation of eosinophils, immature (CD1b−/CD172a−) dendritic cells and B cells. In terms of cytokine expression, an early TNF- α response was stimulated by gene gun delivery of plasmid-associated gold beads, which coincided with an immediate infiltration of neutrophils. However, only pGM-CSF triggered the short-lived expression of GM-CSF (peaking at 4 h) and significant long-term increases in both TNF- α and IL-1 β. pGM-CSF did not affect the expression of the immune response-polarizing cytokines, IL-10 and IL-12. [ABSTRACT FROM AUTHOR]

The article compares education in the human and veterinary fields, outlining key similarities and differences in Great Britain. Both undergraduate programs lead to membership of a professional register. Despite the increase in places in medical schools, it has become relatively more difficult to enter in human than veterinary medicine. Veterinary curricula is in many ways similar to earlier traditional medical curricula. One significant difference between the two professions is the need in the veterinary field for the business side of practice to be taught.

Bronchoalveolar lavage fluid was collected postmortem from the lungs of 113 sheep, and total and differential cell counts were analysed in relation to the presence of gross and microscopic lung pathology. The diffuse lung diseases, maedi and adenomatosis, were both characterised by an increase in overall cellularity and by increases in the percentages of lymphocytes and neutrophils, respectively. Focal parasitic lung disease was characterised by an increase in the percentage of eosinophils and mast cells. Consolidated lung lesions were characterised by a slight increase in cellularity but no change in the differential cell profile. In regions of parasitised and consolidated lungs without lesions the differential cell profile was consistent with focal lung pathology, although the slight increase in cellularity observed in the consolidated regions was not observed in the regions without lesions. A decision tree was developed to facilitate the interpretation and indicate the likely predictive capacity of the differential cytology of the fluid. [ABSTRACT FROM AUTHOR]

The sources and characteristics of endocrine disrupting compounds (EDCs) are reviewed and discussed with respect to their potential effects on farm animal health, welfare and productivity. The importance of certain properties of these compounds in relation to the expression of their biological effects is addressed together with potential routes of exposure. It is concluded that little is known of factors affecting the tissue concentrations of EDCs in farm animals, the concentrations that are required to perturb physiological function in these species, the effects of prolonged exposure to low doses, the effect of cocktails of EDCs and other pollutants or the responses of specific organs and physiological systems that are affected by EDCs. Much of the available information pertaining to EDCs is derived from epidemiological studies of wildlife species and from laboratory animal studies and while these studies have significant limitations, they are considered to be valuable indicators of potential effects in farm animal species. The results of such studies, together with the small amounts of data from studies of ruminants, indicate that there may be significant effects of exposure to environmental levels of EDCs on farm animal health, even although effects are not generally apparent in practice, at this time. [ABSTRACT FROM AUTHOR]

SummaryDifferential expression of ovine CD1 was examined using a combination of reverse transcription–polymerase chain reaction (RT–PCR) with sequence-specific primers and Northern and in situ hybridization techniques. The aim of the study was to establish the patterns of CD1 expression at the molecular level and address questions posed by previous studies in other species regarding expression patterns of CD1. A ‘pan-CD1’ probe based on the exon 4 (alpha 3) region was used in addition to isotype-specific probes for SCD1B (the exon 3 region of clone SCD1B42) and SCD1D (the exon 3 region of clone SCD1D). Widespread expression of CD1 (including thymus, peripheral blood lymphocytes, lung and intestine) was identified using both the exon 4 and SCD1D probe. SCD1B expression was more restricted, being identified in equivalent levels only in the thymus and in scattered populations of dendritic cells. These results highlight the difference in expression patterns between group 1 and group 2 CD1 family members and establish SCD1D as the CD1 family member with the widest pattern of expression, consistent with a differential role for the different CD1 family members. [ABSTRACT FROM AUTHOR]

This paper describes the clinical features, and diagnostic findings of a chronic respiratory condition in 29 West Highland white terriers. Typically, the dogs were coughing chronically, had dyspnoea and tachypnoea of varying severity, and had deteriorated progressively over months to year. The mean (sem) survival time in months from the clinical signs being first noted by the owners was 17.9 (2.3). Most cases had a combination of respiratory signs, but coughing was the predominant sign in 18 cases. Inspiratory crackles were audible on chest auscultation in 28 cases, 10 of which were also wheezing. Rhonchi were the predominant sound in the remaining case. The main radiographic changes were mild to severe increased interstitial markings in all cases, with additional bronchial markings in 14 of the dogs. Right-sided cardiomegaly (cor pulmonale) was recorded in 15. Bronchoscopic findings in 17 of the dogs were either normal or involved a mild airway mucoid reaction in eight. Chronic mucosal changes were observed in eight, but in two this finding was equivocal. Dynamic changes to the lumen of the airway were present in seven cases. No significant haematological or biochemical changes could be detected in 20 cases, but four cases were hypercholestrolaemic. A histopathological assessment of four cases revealed alveolar septal fibrosis to be the predominant change. Prednisolone, with or without bronchodilators, was the most commonly used therapy, and the response was variable. The condition appears to be associated with significant pulmonary interstitial fibrosis of unknown aetiology and has clinical similarities to idiopathic pulmonary fibrosis (cryptogenic fibrosing alveolitis) in human beings. [ABSTRACT FROM PUBLISHER]

Passive immunization of cattle with ovine anti-testosterone antiserum can result in an increased ovulation rate, but the effect is variable and may be influenced by the route of administration. Investigations were made into the pharmacokinetics of these antibodies in cattle when given intravenously (i.v.), subcutaneously (s.c.) or via a combination of these two routes. Serum levels of free residual binding sites were measured by testosterone radioimmunoassay, whilst total circulating ovine IgG was determined using a specific enzyme-linked immunosorbent assay which shows no cross-reactivity with bovine IgG. The biological half-life of the administered antibodies was longer when it was calculated by measuring titre than when it was calculated by measuring IgG. Subcutaneous injection resulted in a significantly longer half-life of IgG than intravenous injection or the combined route, with a concomitant increase in the area under the curve. No significant differences between the half-lives as measured by titre were noted following the various routes of administration, but the mean value following s.c. injection was longest. The choice of route of administration of antiserum for passive immunization can be used to control the timing and duration of effective antibody levels. The results of the present study suggest that the s.c. or combined i.v. and s.c. routes are the preferred methods of passive immunization if an effect of long duration is required. It may be that it is the period over which the maximum level is maintained, rather than the absolute maximum level, which is important for successful immunomodulation of ovulation rate. [ABSTRACT FROM AUTHOR]

The article presents a study which compares student support systems at veterinary schools in Great Britain. It highlights a web-based questionnaire that was electronically delivered to seven veterinary schools in August 2011 which contains questions such as the distance of schools from main campuses and the availability and types of counseling and support services given to students. The research finding indicates that students have the access to counseling facilities on campuses.

The article describes an outbreak of orf in a flock with no known history of the disease and was noted one week after plunge dipping to control blowfly strike in Great Britain. The typical histopathological features of the nodular lesions are hyperplastic epidermis associated with a thick layer of parakeratosis, continuous with a thick serocellular crust containing prominent bacterial colonies. The dermal inflammatory reaction was severe and comprised mixed macrophages and lymphocytes.