1. Stimulation of fibroblast growth in vitro by intermittent radiant warming.
- Author
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Xia, Zhidao, Sato, Akio, Hughes, Margaret A, and Cherry, George W
- Subjects
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WOUND healing , *FIBROBLASTS , *GROWTH - Abstract
A number of clinical studies have suggested that radiant heat improves the healing of selected acute and chronic wounds. The purpose of this study was to investigate in vitro the effect of intermittent radiant heating on the growth of human skin fibroblasts using a radiant heat-producing dressing with a designated temperature of 38 °C. In initial experiments cells were seeded in six well-plates, maintained in culture at 33–34 °C, and warmed daily for three cycles of 1 hour with 1.5 hour intervals. Changes in cell growth and metabolism were determined in sets of triplicate wells by cell counts and a colorimetric assay before and after one week's treatment, After eight days the number of cells indings suggest that the stimulation of cell proliferation induced by intermittent heating in vitro may indicate a possible mechanism contributing to in vivo effects. (WOUND REP REG 2000;8:138–144). [ABSTRACT FROM AUTHOR]
- Published
- 2000
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