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Assessing anthropogenic climate change in a regional context is challenging due to the spatial heterogeneity of climatic variables and is more complicated than at the global scale. Especially in the Tropics, such spatial variations are expected to increase, warranting the identification of homogeneous climatic zones for assessing regional climate change. The present study explores the ability of bioclimatic variables in defining regional climatic zones, and the detection of climate change therein. We hypothesize that the identification of homogeneous climatic zones based on bioclimatic variables could be an effective approach rather than the conventional extreme climate‐based indices to identify climate change signals. To demonstrate the hypothesis, bioclimatic variables representing the generalized climatic characteristics of a tropical river basin were derived from observed gridded datasets of rainfall and temperature. Clusters of homogeneous climatic zones were identified, and their temporal variations were analysed to examine the existence of climate change. The results indicate that despite the spatial heterogeneity in extreme climate‐based indices, the bioclimatic variables‐based approach renders a meaningful representation of the regional climatic pattern. Investigation of bioclimatic zones of the study area helped to identify a shift in its climatic zones with a slant towards drier conditions. Further, future changes in climatic zones were identified from 13 different GCMs that participated in the CMIP6, projecting drier conditions over the basin, with varying spatial extend based on future emission scenarios. The study significantly contributes towards the identification of climatologically fragile regions in changing climate, which is an essential component in developing any regional climate change adaptation and mitigation strategy. [ABSTRACT FROM AUTHOR]