1. Calligonum polygonoides Linnaeus Extract: HPLC-EC and Total Antioxidant Capacity Evaluation.
- Author
-
Gomes, Sara M. C., Fernandes, Isabel P. G., Shekhawat, Narpat Singh, Kumbhat, Sunita, and Oliveira ‐ Brett, Ana Maria
- Subjects
FLAVONOIDS ,HYDROPHILIC interaction liquid chromatography ,CARBON electrodes ,ELECTRODES ,PROCYANIDINS - Abstract
Flavonoids in Calligonum polygonoides Linnaeus extract were separated, detected, and identified by reverse-phase high-performance liquid chromatography (RP-HPLC) with electrochemical detection (EC) in combined isocratic and gradient elution using a glassy carbon or a boron doped diamond electrode. Ultrasonication coupled with a microwave-assisted technique was developed to optimize the extraction of the phenolic compounds. The total antioxidant capacity was quantified using the DPPH
. method and voltammetry. The RP-HPLC-EC led to the detection of nine different flavonoids: catechin, delphinidin, fisetin, myricetin, epicatechin, kuromanin, rutin, callistephin and procyanidin A2, in a single run by direct injection of the sample extract solution. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF