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The rabbit midcingulate cortex that enclosed four cortical areas was immunohistochemically studied using a calcium‐binding protein, parvalbumin, as a neurochemical marker. The distribution of parvalbumin‐immunopositive somata and fibres was similar across all four areas, where they were present mainly in layers 2/3 and 5. However, there were a slightly greater number of the immunopositive structures in the two ventral areas than the two dorsal areas. Similarity in the distribution of parvalbumin‐immunopositive structures across the four areas suggests that neurons expressing parvalbumin may be involved in similar functions across the constituent areas of the rabbit midcingulate cortex. [ABSTRACT FROM AUTHOR]