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Objectives: Although the link between poverty and tuberculosis (TB) is widely recognised, limited studies have investigated the association between neighbourhood factors and TB incidence. Since the factors influencing different episodes of TB might be different, this study focused on the first episode of TB disease (first‐episode TB). Methods: All first episodes in previously linked and geocoded TB notification data from 2007 to 2015 in Cape Town, South Africa, were aggregated at the neighbourhood level and merged with the 2011 census data. We conducted an ecological study to assess the association between neighbourhood incidence of first‐episode TB and neighbourhood factors (total TB burden [all episodes] in the previous year, socioeconomic index, mean household size, mean age, and percentage males) using a negative binomial regression. We also examined the presence of hotspots in neighbourhood TB incidence with the Global Moran's I statistic and assessed spatial dependency in the association between neighbourhood factors and TB incidence using a spatial lag model. Results: The study included 684 neighbourhoods with a median first‐episode TB incidence rate of 114 (IQR: 0–345) per 100,000 people. We found lower neighbourhood socioeconomic index (SEI), higher neighbourhood total TB burden, lower neighbourhood mean household size, and lower neighbourhood mean age were associated with increased neighbourhood first‐episode TB incidence. Our findings revealed a hotspot of first‐episode TB incidence in Cape Town and evidence of spatial dependency in the association between neighbourhood factors and TB incidence. Conclusion: Neighbourhood TB burden and SEI were associated with first‐episode TB incidence, and there was spatial dependency in this association. Our findings can inform targeted interventions to reduce TB in high‐risk neighbourhoods, thereby reducing health disparities and promoting health equity. [ABSTRACT FROM AUTHOR]

Aerosol increases over the 20th century delayed the rate at which Earth warmed as a result of increases in greenhouse gases (GHGs). Aggressive aerosol mitigation policies arrested aerosol radiative forcing from ∼1980 to ∼2010. Recent evidence supports decreases in forcing magnitude since then. Using the approximate partial radiative perturbation (APRP) method, future shortwave aerosol effective radiative forcing changes are isolated from other shortwave changes in an 18‐member ensemble of ScenarioMIP projections from phase 6 of the Coupled Model Intercomparison Project (CMIP6). APRP‐derived near‐term (2020–2050) aerosol forcing trends are correlated with published model emulation values but are 30%–50% weaker. Differences are likely explained by location shifts of aerosol‐impacting emissions and their resultant influences on susceptible clouds. Despite weaker changes, implementation of aggressive aerosol cleanup policies will have a major impact on global warming rates over 2020–2050. APRP‐derived aerosol radiative forcings are used together with a forcing and impulse response model to estimate global temperature trends. Strong mitigation of GHGs, as in SSP1‐2.6, likely prevents warming exceeding 2C since preindustrial but the strong aerosol cleanup in this scenario increases the probability of exceeding 2C by 2050 from near zero without aerosol changes to 6% with cleanup. When the same aerosol forcing is applied to a more likely GHG forcing scenario (i.e., SSP2‐4.5), aggressive aerosol cleanup more than doubles the probability of reaching 2C by 2050 from 30% to 80%. It is thus critical to quantify and simulate the impacts of changes in aerosol radiative forcing over the next few decades. Plain Language Summary: Over the 20th century, fossil fuel burning led to increased concentrations of greenhouse gases (GHGs) and small particles known as aerosols. Aerosols scatter sunlight back to space and enhance cloud brightness and longevity, thus cooling Earth. The amount of warming since the pre‐industrial depends upon both GHGs and aerosol. After ∼1980, air quality improvements led to a reduction in this cooling effect, unmasking some of the GHG potential to warm the planet. This has reinforced the importance of understanding the interplay between aerosols and GHGs in climate projections. To examine this sensitivity, we use a set of global climate model simulations forced by a variety of future GHG and aerosol concentration based on plausible socioeconomic pathways. Shifts in the location of regional aerosol emissions has an impact on the global climate, influencing the accuracy of our predictions for Earth's future warming as measured by the probability of increasing global temperatures by 2C by 2050 compared to pre‐industrial. Under plausible GHG scenarios, aggressive aerosol cleanup policies can more than double the probability of crossing this threshold. This emphasizes the urgency of improving our simulations in order to accurately predict and quantify the impact of aerosols over the next few decades. Key Points: Aerosol forcing changes are isolated from CMIP6 projected scenarios using an approximate partial radiative perturbation methodAerosol‐cloud interactions dominate future aerosol forcing changes from cleanup policies over the period 2020–2050Likelihood of maintaining global warming to below 2C will be strongly reduced by implementing aggressive near‐term aerosol cleanup policies [ABSTRACT FROM AUTHOR]

Precipitation plays an important role in cloud and aerosol processes over the Southern Ocean (SO). The main objective of this study is to characterize SO precipitation properties associated with SO stratocumulus clouds. We use data from the Southern Ocean Clouds Radiation Aerosol Transport Experimental Study (SOCRATES), and leverage observations from airborne radar, lidar, and in situ probes. We find that for the cold‐topped clouds (cloud‐top‐temperature <0°C), the phase of precipitation with reflectivity >0 dBZ is predominantly ice, while reflectivity < −10 dBZ is predominantly liquid. Liquid‐phase precipitation properties are retrieved where radar and lidar are zenith‐pointing. Power‐law relationships between reflectivity (Z) and rain rate (R) are developed, and the derived Z–R relationships show vertical dependence and sensitivity to the presence of droplets with diameters between 10 and 40 μm. Using derived Z–R relationships, a reflectivity‐velocity (ZV) retrieval method, and a radar‐lidar retrieval method, we derive rain rate and other precipitation properties. The retrieved rain rate from all three methods shows good agreement with in‐situ aircraft estimates, with rain rates typically being quite light (<0.1 mm hr−1). We examine the vertical distribution of precipitation properties, and find that rain rate, precipitation number concentration, and precipitation liquid water all decrease as one gets closer to the surface, while precipitation size and distribution width increases. We also examine how cloud base rain rate (RCB) depends on cloud depth (H) and aerosol concentration (Na) for particles with a diameter greater than 70 nm, and find that RCB is proportional to H3.1Na−0.8 ${H}^{3.1}\,{N}_{a}^{-0.8}$. Plain Language Summary: Precipitation plays an important role over the Southern Ocean (SO), such as transferring water from the atmosphere to the ocean, and affecting clouds and aerosols (tiny airborne particles). This study aims to characterize SO precipitation properties using aircraft data that can count the number and size of cloud and precipitation droplets, as well as lidar and radar that measure light and microwaves respectively reflected by droplets. Using information from lidar, we can distinguish the precipitation phase, and we find that ice precipitation is more frequent when the amount of reflected energy measured by the radar (radar reflectivity) is larger than a certain threshold. We derived relationships between rain rate and radar reflectivity. We also find the precipitation properties inferred from radar and lidar data compare well with direct measurements from the aircraft, and the precipitation tends to be very light. We study how precipitation properties vary vertically, and find that as one gets closer to the surface, there is a decrease in precipitation droplet number and water, while there is an increase in the average size of droplets. We also find that rain rate depends on how thick the clouds are and the number of aerosols, consistent with theoretical expectations. Key Points: Liquid‐phase precipitation retrievals show good agreement with in situ observations and feature the prevalence of light rainReflectivity to rain rate relationships are developed, showing vertical dependence and sensitivity to the intermediate‐sized dropsThe below‐cloud precipitation phase with radar reflectivity >0 dBZ is mostly ice, while radar reflectivity <−10 dBZ is mostly liquid [ABSTRACT FROM AUTHOR]

Background: The Platelet function analyzer‐200 (PFA‐200) can determine the effect of clopidogrel in cats, but analysis traditionally must be performed at point‐of‐care (POC). The ability to ship samples of blood to a laboratory would allow widespread access. Objectives: We aimed to validate the shipping of blood samples for PFA‐200 analysis in cats to determine the effect of clopidogrel. Methods: Twenty healthy cats and 10 cats receiving clopidogrel were recruited. Blood was collected from cats and aliquoted into two samples, one was analyzed at POC within 2 hours using the PFA‐200, and the other was packaged and transported to a location 4 km away, stored, and transported back to the lab for analysis the following day. Results: Median closure times (CTs) with the collagen/adenosine diphosphate (COL/ADP) cartridge in healthy cats were 51.5 seconds (POC) and 78.8 seconds (shipped), which were significantly different (P < 0.001), and for cats on clopidogrel, median CTs were 147.5 seconds (POC) and 190 seconds (shipped), which were not significantly different (P = 0.131). Median CTs with the P2Y cartridge in healthy cats were 50.5 seconds (POC) and 64.9 seconds (shipped), which were significantly different (P = 0.03), and in cats receiving clopidogrel, median CTs were 300 seconds (POC) and 300 seconds (shipped) which were not significantly different (P = 1.000). Reference intervals for CTs differed for COL/ADP at POC (19.8–89.7 seconds) and shipped (50.9–161.6 seconds) and for P2Y at POC (35.5–118.8 seconds) and shipped (35.1–108.9 seconds). Receiver operating characteristics showed similar areas under the curve (AUCROCs) regarding the effect of clopidogrel for COL/ADP at POC (0.994 seconds) and shipped (0.932) and for P2Y at POC (0.904 seconds) and shipped (0.975 seconds). When classifying for the presence of clopidogrel effects, Cohen's Kappa was 0.62 for COL/ADP and 1.00 for P2Y. Conclusions: Shipping blood samples for PFA analysis are feasible with similar performance to POC analyses for determining the effect of clopidogrel in cats. [ABSTRACT FROM AUTHOR]

Background: The Platelet function analyzer‐200 can determine the effect of clopidogrel in cats. Flow obstruction is an error that causes uninterpretable results. Closure curves and parameters initial flow rate (IF) and total volume (TV) are displayed by the PFA‐200 and may allow interpretation of results in cases of flow obstruction. The primary hemostasis components (PHC) are calculated values that normalize these parameters. Objectives: To determine if closure curves and research parameters allow detecting the effect of clopidogrel in cases of flow obstruction. Methods: A review of closure curves identified those with flow obstruction and paired analysis that did not. Non‐flow–obstructed curves were used to categorize curves with respect to clopidogrel effects. IF, TV, PHC(1), and PHC(2) were evaluated to determine if these could be used to categorize if a sample exhibited the effects of clopidogrel. Curves were visually analyzed, and characteristics identified that were more common with or without the effect of clopidogrel. Visual analysis of curves was performed by blinded observers to determine if a visual analysis was able to predict the effect of clopidogrel. Results: Analysis of parameters was able to predict closure or non‐closure in flow‐obstructed curves. TV, PHC(1), and PHC(2) had area under the curve of the receiver operating characteristics of 0.79, 0.79, and 0.87. Visual curve analysis was unable to predict closure, with an average accuracy of only 55%, among three reviewers. Agreement between reviewers was poor (Fleiss' Kappa 0.06). Conclusions: Visual curve analysis was unable to determine the effect of clopidogrel in flow‐obstructed samples. Numerical parameters were able to detect the effect of clopidogrel with a high degree of accuracy in flow‐obstructed samples. [ABSTRACT FROM AUTHOR]

Background: Platelet function testing is important for monitoring the effects of antiplatelet therapy but is not readily used due to time constraints for testing and the need for specialized equipment. Objectives: This study evaluated the effects of various storage methods on selected platelet function tests to determine if delayed platelet function testing is feasible in canine blood samples. Our hypotheses were that platelet function would not decline during storage and, thus, no differences in test results would be found over time. Methods: Thirteen healthy dogs were studied. Citrated blood samples were tested with a Platelet Function Analyzer‐200 (PFA), which mimics high‐shear conditions, using P2Y and CADP cartridges, after being held at room temperature for 2 h and refrigerated for 24 and 48 h. Plateletworks (PW), which measures aggregation based on platelet counting, was performed on an optical hematology analyzer using 10‐min‐old native samples, citrated samples held at room temperature for 3–4 h and refrigerated for 24 and 48 h, and samples stored in the preservative solution, AGGFix, up to 7 days. Results: PFA closure times increased with storage, especially with the P2Y cartridge. Median aggregation with fresh PW was 94%, and this was maintained at all time points (range of median values 88%–94%). Most samples showed decreased, yet still robust (>70%), aggregation with longer storage. Spontaneous aggregation in citrate was noted in most dogs. AGGFix stabilized platelet aggregates to allow for delayed testing. Conclusions: Delayed platelet function testing is feasible, but ranges of expected values may differ from tests using fresh samples. [ABSTRACT FROM AUTHOR]

Magnesian Limestone zoned dolomite (red and non-luminescent zones) now partly dedolomitized), calcite (yellow luminescence), blue luminescent albite has non-luminescent overgrowths) Mansfield Quarry, Nottinghamshire. Modern tropical carbonate sediments are generally aragonite and high-Mg calcite with high porosities (Enos & Swatsky, [16]), yet ancient carbonates (Miocene and older) are generally calcite or dolomite with far lower porosity. Dissolution of aragonite and Mg calcite with precipitation of calcite cements with 2-8 mol% MgCO SB 3 sb occur in sea water undersaturated with respect to aragonite and high-Mg calcite, but supersaturated with respect to low-Mg calcite. The Xiong et al. compilation shows that the cycles of aragonite/high-Mg calcite were more variable than previously recognised, with the first inferred transition from a high-Mg calcite to low-Mg calcite sea occurring during early Cambrian stage 3. [Extracted from the article]

Myelodysplastic syndrome (MDS) and acute myeloid leukaemia (AML) are primary myeloid neoplasms in dogs generally considered to have a poor outcome. In this study, we assessed toxicity, efficacy and outcome of concurrent administration of doxorubicin and cytarabine in 11 dogs with myeloid neoplasia. Bone marrow specimens were reviewed by three pathologists and classified as either MDS (n = 2), high grade MDS/early AML (MDS/AML; n = 4) or AML (n = 5). The median number of treatment cycles was 5 (range 1–9) and resolution of cytopenia was reported in 7 of 11 dogs including 2 dogs with MDS, 2 dogs with MDS/AML, and 3 dogs with AML. The median duration of remission in the seven responders was 344 days (range 109–1428) and the median overall survival for all dogs was 369 days. Adverse events consisted of predominantly low‐grade gastrointestinal illness and myelosuppression. Three dogs developed grade V toxicity manifesting with heart failure (n = 2) at 369 and 1170 days after diagnosis and acute gastrointestinal side effects (n =1). Despite a limited sample size, these results suggest that a doxorubicin and cytarabine protocol may be considered as a therapeutic option in dogs with myeloid neoplasia. Protocol safety, in particular regarding myocardial toxicity, and efficacy should be further investigated. [ABSTRACT FROM AUTHOR]

Background: Platelet function testing in cats allows determination of clopidogrel effect. Plateletworks assesses aggregation based on decreasing platelet counts on hematology analyzers in response to agonists. It has not been validated for the IDEXX ProCyte Dx analyzer. Ideal time to perform analysis and the utility of other platelet parameters have not been fully assessed. Objectives: To validate Plateletworks ADP on the ProCyte Dx, to investigate the utility of various platelet parameters using Plateletworks ADP, and determine the ideal time to perform analysis. Animals: Twenty healthy cats recruited from the general population used for transference of reference intervals to a new analyzer, and 10 cats receiving clopidogrel to determine clopidogrel effect. Methods: Plateletworks ADP using the ProCyte Dx and ADVIA 2120i analyzer was run simultaneously in both healthy cats and cats receiving clopidogrel, and CBC results at different timepoints were compared between analyzers. Results: Aggregation was significantly different (P <.001) between analyzers. Cohen's kappa showed almost perfect agreement for determination of clopidogrel effect, and the area under the curve of the receiver operating characteristic was 1.0. Lower limits of the aggregation reference interval in healthy cats were 28.8% on the ProCyte Dx and 12.5% on the ADVIA 2120i. Coefficients of variation for platelet parameters were not different between analyzers. No significant changes in mean platelet volume, plateletcrit, large platelets, and mean platelet component were identified. No significant change in aggregation was observed within the first hour after phlebotomy. Conclusions and Clinical Importance: Our study validated the Plateletworks ADP system on the ProCyte Dx analyzer. Samples may be analyzed up to 1 h after collection. [ABSTRACT FROM AUTHOR]

The Arctic is warming at almost four times the global rate. An estimated sixty percent of greenhouse‐gas‐induced Arctic warming has been offset by anthropogenic aerosols, but the contribution of aerosols to radiative forcing (RF) represents the largest uncertainty in estimating total RF, largely due to unknown preindustrial aerosol abundance. Here, sulfur isotope measurements in a Greenland ice core show that passive volcanic degassing contributes up to 66 ± 10% of preindustrial ice core sulfate in years without major eruptions. A state‐of‐the‐art model indicates passive volcanic sulfur emissions influencing the Arctic are underestimated by up to a factor of three, possibly because many volcanic inventories do not include hydrogen sulfide emissions. Higher preindustrial volcanic sulfur emissions reduce modeled anthropogenic Arctic aerosol cooling by up to a factor of two (+0.11 to +0.29 W m−2), suggesting that underestimating passive volcanic sulfur emissions has significant implications for anthropogenic‐induced Arctic climate change. Plain Language Summary: Sulfate aerosols are particles in the atmosphere that have a net cooling effect on the climate. One of the most uncertain aspects of climate modeling is the abundance of sulfate aerosols during the preindustrial era. Without knowing the amount of sulfate aerosols during the preindustrial, it is difficult to estimate how much anthropogenic sulfate aerosols have offset warming from anthropogenic greenhouse gases. In this study, we examine preindustrial sulfate aerosols in a Greenland ice core. We find that sulfate aerosols from passive (i.e., non‐eruptive) volcanic degassing contribute almost two thirds of preindustrial Arctic sulfate aerosols in years without major volcanic eruptions. We compare this result to a state‐of‐the‐art global model and find that most climate models use a volcanic emissions inventory that underestimates preindustrial passive volcanic sulfur emissions. That volcanic inventory only includes one type of sulfur emission (sulfur dioxide), but studies have shown that volcanoes emit hydrogen sulfide, which can also form sulfate aerosols. We show that higher emissions of volcanic sulfur during the preindustrial era decrease the estimated cooling effect of anthropogenic aerosols during the industrial era. Thus, the underestimate of preindustrial volcanic emissions in current climate models has significant implications for anthropogenic climate change in the Arctic. Key Points: Sulfur isotopes in a Greenland ice core show that passive volcanic degassing contributes 66% of preindustrial Arctic sulfateThe volcanic inventory used by most climate models underestimates passive degassing, possibly due to missing hydrogen sulfide emissionsElevated preindustrial passive volcanic degassing reduces the estimated cooling effect of anthropogenic sulfate in the Arctic [ABSTRACT FROM AUTHOR]

Background: Lipid disorders are common in captive psittacine birds, but associated changes in blood lipids and lipoproteins have not been well characterized. The Quaker parrot is prone to dyslipidemia and has been extensively used as an experimental model. Objectives: We aimed to study the effects of a 0.3% cholesterol diet and a 20% fat diet on plasma lipids and lipoproteins in Quaker parrots. Methods: Two crossover studies were performed with each diet. During each study, 12 parrots were divided into two groups fed the treatment or control diet for 2 weeks. After a 2‐month wash‐out period, the groups were reversed. At the end of each period, plasma lipidomics and lipoprotein profiling were performed. Data were analyzed by univariate tests adjusted for false discovery rates, volcano plots, and enrichment analyses. Results: The cholesterol diet induced changes in many plasma lipids and lipoproteins. Total cholesterol and cholesteryl esters were significantly and markedly elevated. Ceramides were the second subclass of lipids that were elevated. Several glycerophosphocholines, sphingomyelins, and one diacylglycerol were also significantly elevated, albeit to a lesser magnitude. All lipoproteins were elevated, with the greatest increase seen in non‐HDL. The fat diet mainly resulted in a decrease in plasma glycerolipids and an increase in acylcarnitines. Lipoprotein plasma levels remained unchanged. Conclusions: Quaker parrots fed a 0.3% cholesterol diet showed profound and complex dyslipidemic changes that could be used to further study lipid disorders and their management in psittacine birds. A 20% fat diet higher in n‐6 polyunsaturated fatty acids did not lead to dyslipidemia. [ABSTRACT FROM AUTHOR]

Using a cohort study design, we analysed 17 diagnoses and 9 interventions (including critical care admission) as a composite measure of severe maternal morbidity for pregnancies recorded over 14 years in Scotland. There were 762,918 pregnancies, of which 7947 (10 in 1000 pregnancies) recorded 9345 severe maternal morbidity events, 2802 episodes of puerperal sepsis being the most common (30%). Severe maternal morbidity incidence increased from 9 in 1000 pregnancies in 2012 to 17 in 1000 pregnancies in 2018, due in part to puerperal sepsis recording. The odds ratio (95%CI) for severe maternal morbidity was higher for: older women, for instance 1.22 (1.13-1.33) for women aged 35-39 years and 1.44 (1.27-1.63) for women aged > 40 years compared with those aged 25-29 years; obese women, for instance 1.13 (1.06-1.21) for BMI 30-40 kg.m-2 and 1.32 (1.15-1.51) for BMI > 40 kg.m-2 compared with BMI 18.5-24.9 kg.m-2 ; multiple pregnancy, 2.39 (2.09-2.74); and previous caesarean delivery, 1.52 (1.40-1.65). The median (IQR [range]) hospital stay was 3 (2-5 [1-8]) days with severe maternal morbidity and 2 (1-3 [1-5]) days without. Forty-one women died during pregnancy or up to 42 days after delivery, representing mortality rates per 100,000 pregnancies of about 365 with severe maternal morbidity and 1.6 without. There were 1449 women admitted to critical care, 807 (58%) for mechanical ventilation or support of at least two organs. We recorded an incidence of severe maternal morbidity higher than previously published, possibly because sepsis was coded inaccurately in our databases. Further research may determine the value of this composite measure of severe maternal morbidity. [ABSTRACT FROM AUTHOR]

Urothelial carcinoma (UC) is the most common urinary tumour in dogs. Despite a range of treatment options, prognosis remains poor in dogs. In people, breakthroughs with checkpoint inhibitors have established new standards of care for muscle‐invasive bladder cancer patients and elevated levels of programmed cell death protein 1 (PD‐1) suggest immune checkpoint blockade may be a novel target for therapy. The goal of this study was to determine if canine UC patients express elevated levels of lymphocyte‐specific PD‐1 and/or urinary cytokine biomarkers compared to healthy dogs. Paired blood and urine were evaluated in 10 canine UC patients, five cystitis patients and 10 control dogs for lymphocyte‐specific PD‐1 expression via flow cytometry and relative cytokine expression. In UC patients, PD‐1 expression was significantly elevated on CD8+ lymphocytes in urine samples. UC patients had a higher CD4:CD8 ratio in their urine compared to healthy dogs, however, there was no significant variation in the CD8:Treg ratio between any group. Cystitis patients had significantly elevated levels of CD4+ T cells, CD8+ T cells and Tregs in their blood samples compared to UC patients and healthy dogs. Cytokine analysis demonstrated significant elevations in urinary cytokines (granulocyte‐macrophage colony‐stimulating factor, interferon‐gamma [IFN‐γ], interleukin (IL)‐2, IL‐6 IL‐7, IL‐8 and IL‐15, IP‐10, KC‐like, IL‐18, monocyte chemoattractant protein‐1 and tumour necrosis factor‐alpha). Several of these cytokines have been previously correlated with both lymphocyte‐specific PD‐1 expression (IFN‐γ, IL‐2, IL‐7 and IL‐15) in muscle‐invasive urothelial carcinoma in humans. Our results provide evidence of urinary lymphocyte PD‐1 expression and future studies could elucidate whether veterinary UC patients will respond favourably to anti‐PD‐1 immune checkpoint inhibitor therapy. [ABSTRACT FROM AUTHOR]

Cloud droplet number concentration (Nd) is a key microphysical property that is largely controlled by the balance between sources and sinks of aerosols that serve as cloud condensation nuclei (CCN). Despite being a key sink of CCN, the impact of coalescence scavenging on Southern Ocean (SO) cloud is poorly known. We apply a simple source‐and‐sink budget model based on parameterizations to austral summer aircraft observations to test model behavior and examine the relative influence of processes that determine Nd in SO stratocumulus clouds. The model predicts Nd with little bias and a correlation coefficient of ∼0.7 compared with observations. Coalescence scavenging is found to be an important sink of CCN in both liquid and mixed‐phase precipitating stratocumulus and reduces the predicted Nd by as much as 90% depending on the precipitation rate. The free tropospheric aerosol source controls Nd more strongly than the surface aerosol source during austral summer. Plain Language Summary: Low altitude stratiform clouds are ubiquitous over the Southern Ocean (SO) and have a profound climate impact through reflecting sunlight back to space, cooling the earth. The number of water droplets in a given volume (the concentration) is a key variable that determines the reflective ability of the cloud. The cloud droplet number concentration (Nd) is largely set by the number of aerosols (tiny airborne particles) on which water can condense and is controlled by the balance between sources that generate aerosols (e.g., ocean biology, sea spray) and sinks which remove aerosols from the atmosphere. The formation of liquid precipitation in clouds (drizzle) is a strong sink of aerosols because each precipitation droplet is created through the merger of many cloud droplets (i.e., coalescence) where each cloud droplet contains at least one aerosol particle. Here, we test a simple source‐and‐sink model to predict SO Nd using aircraft measurements from a recent aircraft campaign in the SO during austral summer. We find this model can predict cloud droplet number well and that liquid precipitation processes are important in controlling droplet number concentration (and thus cloud reflectance) over the SO. Key Points: A simple source‐and‐sink budget model accurately predicts in situ observations of Southern Ocean (SO) cloud droplet number concentration (Nd)Coalescence scavenging by liquid droplets is a dominant sink of SO cloud condensation nucleiFree tropospheric aerosol controls Nd more strongly than surface aerosol during austral summer [ABSTRACT FROM AUTHOR]

The shape of soil‐mantled hillslopes is typically attributed to erosion rate and the transport efficiency of the various processes that contribute to soil creep. While climate is generally hypothesized to have an important influence on soil creep rates, a lack of uniformity in the measurement of transport efficiency has been an obstacle to evaluating the controls on this important landscape parameter. We addressed this problem by compiling a data set in which the transport efficiency has been calculated using a single method, the analysis of hilltop curvatures using 1‐m LiDAR data, and the erosion rates have also been determined via a single method, in‐situ cosmogenic 10Be concentrations. Moreover, to control for lithology, we chose sites that are only underlain by resistant bedrock. The sites span a range of erosion rates (6–922 mm/kyr), mean annual precipitation (39–320 cm/yr), and aridity index (0.08–1.38). Surprisingly, we find that hilltop curvature varies with the square root of erosion rate, whereas previous studies predict a linear relationship. In addition, we find that the inferred transport coefficient also varies with the square root of erosion rate but is insensitive to climate. We explore various mechanisms that might link the transport coefficient to the erosion rate and conclude that present theory regarding soil‐mantled hillslopes is unable to explain our results and is, therefore, incomplete. Finally, we tentatively suggest that processes occurring in the bedrock (e.g., fracture generation) may play a role in the shape of hillslope profiles at our sites. Key Points: Hilltop curvature at our sites does not vary linearly with erosion rate, as predicted by theoryThe inferred transport coefficient appears to be insensitive to climateProcesses affecting the underlying bedrock may control the shape of soil‐mantled hillslopes [ABSTRACT FROM AUTHOR]

Hepatitis B virus (HBV) can be transmitted from organ donor to recipient, but details of transmission events are not widely published. The Disease Transmission Advisory Committee (DTAC) evaluated 105 cases of potential donor derived transmission events of HBV between 2009‐2017. Proven, probable or possible transmission of HBV occurred in 25 (23.8%) cases. Recipients of liver grafts were most commonly infected (20 of 21 exposed recipients) compared to 9 of 21 exposed non‐hepatic recipients. Eleven of 25 donors were HBV core antibody (HBcAb) positive/HBV surface antigen (HBsAg) negative and infected 8/20 recipients. Of the 10 liver recipients and 1 liver‐kidney recipient who received organs from these donors: six were not given antiviral prophylaxis, two developed infection after antiviral prophylaxis was discontinued, two developed HBV while on lamivudine prophylaxis, one was on antiviral prophylaxis and did not develop HBV viremia or antigenemia. One recipient of a HBcAb positive/HBsAg negative kidney developed active HBV infection. Unexpected donor‐derived transmission of HBV was a rare event in reports to DTAC, but was often detected in the recipient late post‐transplant. Six of 11 recipients (54.5%) of a liver from a HBcAb positive donor did not receive prophylaxis; all of these were potentially preventable with the use of anti‐viral prophylaxis. [ABSTRACT FROM AUTHOR]

Objective: To explore parents' lived experiences of care and support following stillbirth in urban and rural health facilities. Design: Qualitative, interpretative, guided by Heideggerian phenomenology. Setting: Nairobi and Western Kenya, Kampala and Central Uganda. Sample: A purposive sample of 75 women and 59 men who had experienced the stillbirth of their baby (≤1 year previously) and received care in the included facilities. Methods: In‐depth interviews, analysed using Van Manen's reflexive approach. Results: Three main themes were identified; parents described devastating impacts and profound responses to their baby's death. Interactions with health workers were a key influence, but poor communication, environmental barriers and unsupportive facility policies/practices meant that needs were often unmet. After discharge, women and partners sought support in communities to help them cope with the death of their baby but frequently encountered stigma engendering feelings of blame and increasing isolation. Conclusions: Parents in Kenya and Uganda were not always treated with compassion and lacked the care or support they needed after the death of their baby. Health workers in Kenya and Uganda, in common with other settings, have a key role in supporting bereaved parents. There is an urgent need for context and culturally appropriate interventions to improve communication, health system and community support for African parents. Health‐system response and community support for parents after stillbirth in Kenya and Uganda are inadequate. Health‐system response and community support for parents after stillbirth in Kenya and Uganda are inadequate. [ABSTRACT FROM AUTHOR]

Background: Increased serum interleukin 17 (IL‐17) concentration has been associated with the immunopathogenesis of autoimmune hemolytic anemia in humans. No data are available about IL‐17 in immune‐mediated hemolytic anemia (IMHA) of dogs. Objectives: Monitor changes in serum IL‐17 concentration during the acute stages of IMHA in dogs, compared with results in healthy dogs, and its relationship with outcome. Animals Thirty‐one client‐owned dogs with primary IMHA and 27 healthy dogs. Methods: Quantification of serum IL‐17 concentration using a commercially available ELISA kit at the time of admission (D0), after 48 hours (D2) and after 96 hours (D4) as compared to concentration in healthy dogs. The IMHA dogs were classified as survivors if discharged from hospital, or nonsurvivors for any cause of in‐hospital mortality. Results: Mean serum IL‐17 concentration was higher in dogs with IMHA on admission compared with healthy dogs (D0), but this difference was not significant (mean, 19.52 pg/mL vs 10.52 pg/mL, respectively, P =.17). Throughout hospitalization, serum IL‐17 concentration significantly decreased in survivors. Serum IL‐17 concentration at D0 was not different between survivors and nonsurvivors, but surviving dogs had significantly lower serum IL‐17 concentration at D2 and D4 (P =.04 and P =.004, respectively) compared with nonsurviving dogs. No correlation was found between serum IL‐17 concentration and serum total bilirubin or lactate concentrations or CBC parameters. Conclusion and Clinical Importance: Serum IL‐17 concentration remained significantly higher in nonsurviving IMHA dogs whereas it significantly decreased during hospitalization in survivors, making serum IL‐17 concentration a potential biomarker for severity and response to treatment in IMHA. [ABSTRACT FROM AUTHOR]

At regional to local scales internal variability is expected to be a dominant source of uncertainty in analyzing precipitation extremes and mean precipitation even far into the 21st century. A debated topic is whether a faster increase in subdaily precipitation extremes can be expected. Here we analyzed seasonal maximum precipitation in various time steps (3 hr, days, and 5 days) from a high‐resolution 50‐member large‐ensemble (CRCM5‐LE) and compared them to changes in mean precipitation over Europe. Our results show that the magnitude of change in extreme precipitation varies for season and duration. Subdaily extremes increase at higher rates than daily extremes and show higher scaling with temperature. Northern Europe shows widespread scaling above Clausius‐Clapeyron of subdaily extremes in all seasons and for daily extremes in winter/spring. Scaling above Clausius‐Clapeyron is also visible over Eastern Europe in winter/spring. For most regions and seasons the forced response emerges from the internal variability by midcentury. Plain Language Summary: The knowledge on how and why the intensity and frequency of extremes changes is critical to a resilient society. Our adaptive measures that are currently in place are based on observed extremes of the past. We know that observations are only one realization of a chaotic system and that the climate system is altered by natural variations, and anthropogenic contributions. Since we can only measure one realization of the world, we need climate models to investigate the influence of natural variability and anthropogenic factors. In this study we focus on the contribution of natural variability and the detection of regional patterns of changes in extreme precipitation. We used regional climate simulations, driven by multiple runs of global climate simulations under the same emission scenario, but with slight changes at the start of the simulation to imitate the butterfly effect of the climate system and simulate natural variability. We have found that natural variability plays a dominant role in the first half of the 21st century. But we have also found that subdaily extreme precipitation is increasing at a higher rate than daily extremes and that some of this change can be attributed to the warming of the atmosphere. Key Points: Subdaily extreme precipitation intensifies faster than daily extremes and mean precipitationOn regional scales internal variability remains a dominant source of uncertainty until the end of the 21st centuryWidespread scaling rates of subdaily extremes above Clausius‐Clapeyron over Northern and Eastern Europe [ABSTRACT FROM AUTHOR]

Objectives: To compare markers of inflammation after transfusion of leukoreduced (LR) packed RBCs (pRBCs) versus non‐LR pRBCs in dogs with critical illness requiring blood transfusion, and to report survival to discharge and rates of transfusion reactions in these dogs. Design: Prospective randomized blinded clinical study June 2014–September 2015. Setting: University veterinary teaching hospital. Animals: Twenty‐three client‐owned critically ill dogs, consecutively enrolled. Interventions: Dogs requiring a single pRBC transfusion were randomized into the LR or non‐LR pRBC group. Exclusion criteria included: requirement for multiple blood products, history of previous blood transfusion, and administration of anti‐inflammatory or immunosuppressive medication prior to enrollment. Measurements: Blood samples were obtained immediately prior to transfusion, then 2 and 24 hours following transfusion. Parameters measured at each time point included: PCV, WBC count, segmented and band neutrophil counts, fibrinogen, and plasma lactate and C‐reactive protein concentrations. Acute patient physiologic and laboratory evaluation fast score was calculated on admission. Results: Eleven dogs were included in the LR group and 12 in the non‐LR group; scores of illness severity were not significantly different between groups. Total WBC count was significantly higher in the non‐LR versus LR group 24 hours following pRBC transfusion, but this difference was not evident 2 hours following transfusion. No other inflammatory parameters at any time point were significantly different between LR versus non‐LR pRBC transfused dogs. Survival rates to discharge for LR and non‐LR groups were 8/11 and 9/12, respectively. Acute transfusion reactions were identified in 1/11 and 2/12 dogs in the LR and non‐LR group, respectively. All transfused blood was stored ≤12 days. Conclusions: Most markers of inflammation did not significantly increase following transfusion of LR versus non‐LR pRBCs stored ≤12 days in ill dogs. Further prospective, randomized trials are needed in clinically ill dogs to determine the benefit of prestorage leukoreduction. [ABSTRACT FROM AUTHOR]

Summary: Eating patterns involving intermittent energy restriction (IER) include 'intermittent fasting' where energy intake is severely restricted for several 'fasting' days per week, with 'refeeding' days (involving greater energy intake than during fasting days) at other times. Intermittent fasting does not improve weight loss compared to continuous energy restriction (CER), where energy intake is restricted every day. We hypothesize that weight loss from IER could be improved if refeeding phases involved restoration of energy balance (i.e. not ongoing energy restriction, as during intermittent fasting). There is some evidence in adults with overweight or obesity showing that maintenance of a lower weight may attenuate (completely or partially) some of the adaptive responses to energy restriction that oppose ongoing weight loss. Other studies show some adaptive responses persist unabated for years after weight loss. Only five randomized controlled trials in adults with overweight or obesity have compared CER with IER interventions that achieved energy balance (or absence of energy restriction) during refeeding phases. Two reported greater weight loss than CER, whereas three reported similar weight loss between interventions. While inconclusive, it is possible that achieving energy balance (i.e. avoiding energy restriction or energy excess) during refeeding phases may be important in realizing the potential of IER. [ABSTRACT FROM AUTHOR]

Abstract: We show a quasi‐irreversible shutdown (hysteresis) of the Atlantic meridional overturning circulation (AMOC) in an unfluxadjusted global climate model with an eddy‐permitting ocean. This global climate model is a prototype of one submitted to the sixth Coupled Model Intercomparison Project and is the most comprehensive model to show this behavior. The AMOC has a resilience timescale for a given hosing: If the hosing exceeds the critical hosing for collapse for only a limited time, the AMOC recovers when the hosing is stopped. Beyond the critical time, when the hosing stops the AMOC stays in a weak state with no recovery within 200 years. We show that simple, observable metrics such as AMOC strength and mixed layer depth can indicate whether this critical resilience time has passed and that the mechanism controlling the presence of recovery is a positive advective feedback. [ABSTRACT FROM AUTHOR]

Abstract: Background: Gut microbiota may play a role in egg allergy. We sought to examine the association between early‐life gut microbiota and egg allergy. Methods: We studied 141 children with egg allergy and controls from the multicenter Consortium of Food Allergy Research study. At enrollment (age 3 to 16 months), fecal samples were collected, and clinical evaluation, egg‐specific IgE measurement, and egg skin prick test were performed. Gut microbiome was profiled by 16S rRNA sequencing. Analyses for the primary outcome of egg allergy at enrollment, and the secondary outcomes of egg sensitization at enrollment and resolution of egg allergy by age 8 years, were performed using Quantitative Insights into Microbial Ecology, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States, and Statistical Analysis of Metagenomic Profiles. Results: Compared to controls, increased alpha diversity and distinct taxa (PERMANOVA P = 5.0 × 10−4) characterized the early‐life gut microbiome of children with egg allergy. Genera from the Lachnospiraceae, Streptococcaceae, and Leuconostocaceae families were differentially abundant in children with egg allergy. Predicted metagenome functional analyses showed differential purine metabolism by the gut microbiota of egg‐allergic subjects (Kruskal‐Wallis Padj = 0.021). Greater gut microbiome diversity and genera from Lachnospiraceae and Ruminococcaceae were associated with egg sensitization (PERMANOVA P = 5.0 × 10−4). Among those with egg allergy, there was no association between early‐life gut microbiota and egg allergy resolution by age 8 years. Conclusion: The distinct early‐life gut microbiota in egg‐allergic and egg‐sensitized children identified by our study may point to targets for preventive or therapeutic intervention. [ABSTRACT FROM AUTHOR]

Background: Thrombin plays a central role in hemostasis and thrombosis. Calibrated automated thrombography (CAT), a thrombin generation assay, may be a useful test for hemostatic disorders in dogs. Objectives: To describe CAT results in a group of healthy dogs, and assess preanalytical variables and biological variability. Animals: Forty healthy dogs were enrolled. Methods: Lag time (Lag), time to peak (ttpeak), peak thrombin generation (peak), and endogenous thrombin potential (ETP) were measured. Direct jugular venipuncture and winged‐needle catheter‐assisted saphenous venipuncture were used to collect samples from each dog, and results were compared between methods. Sample stability at −80°C was assessed over 12 months in a subset of samples. Biological variability of CAT was assessed via nested ANOVA using samples obtained weekly from a subset of 9 dogs for 4 consecutive weeks. Results: Samples for CAT were stable at −80°C over 12 months of storage. Samples collected via winged‐needle catheter venipuncture showed poor repeatability compared to direct venipuncture samples; there was also poor agreement between the 2 sampling methods. Intra‐individual variability of CAT parameters was below 25%; inter‐individual variability ranged from 36.9% to 78.5%. Conclusions: Measurement of thrombin generation using CAT appears to be repeatable in healthy dogs, and samples are stable for at least 12 months when stored at −80°C. Direct venipuncture sampling is recommended for CAT. Low indices of individuality suggest that subject‐based reference intervals are more suitable when interpreting CAT results. [ABSTRACT FROM AUTHOR]

Abstract: Synthetic multidecadal spaceborne lidar records are used to examine when a cloud response to anthropogenic forcing would be detectable from spaceborne lidar observations. The synthetic records are generated using long‐term cloud changes predicted by two Coupled Model Intercomparison Program 5 models seen through the COSP/lidar (CFMIP, Cloud Feedback Model Intercomparison Project, Observation Simulators Package) and cloud interannual variability observed by the CALIPSO (Cloud Aerosol Lidar and Infrared Pathfinder Satellite Observations) spaceborne lidar during the past decade. CALIPSO observations do not show any significant trend yet. Our analysis of the synthetic time series suggests that the tropical cloud longwave feedback and the Southern Ocean cloud shortwave feedback might be constrained with 70% confidence with, respectively, a 20‐year and 29‐year uninterrupted lidar‐in‐space record. A 27‐year record might be needed to separate the two different model predictions in the tropical subsidence clouds. Assuming that combining the CALIPSO and Earth‐CARE (Earth Clouds, Aerosols and Radiation Explorer) missions will lead to a spaceborne lidar record of at least 16 years, we examine the impact of gaps and calibration offsets between successive missions. A 2‐year gap between Earth‐CARE and the following spaceborne lidar would have no significant impact on the capability to constrain the cloud feedback if all the space lidars were perfectly intercalibrated. Any intercalibration shift between successive lidar missions would delay the capability to constrain the cloud feedback mechanisms, larger shifts leading to longer delays. [ABSTRACT FROM AUTHOR]

Objectives: To compare haemostatic function in healthy dogs after treatment with low‐dose aspirin alone, fish oil alone or a combination of these two therapies. Materials and Methods: Double‐blinded randomised controlled clinical trial on 16 healthy client‐owned dogs. Comprehensive haemostatic testing was performed at baseline and after 7 days of therapy with low‐dose aspirin in all dogs. Following a 14‐day washout, six dogs received fish oil, and nine dogs received combination therapy of aspirin plus fish oil; haemostatic testing was performed before and at 7 and 28 days after treatment initiation. Results: Aspirin was associated with significantly decreased platelet function as measured by a collagen‐epinephrine cartridge and inhibited arachidonic acid‐induced whole‐blood platelet aggregometry. Fish oil alone did not significantly affect any haemostatic tests. The combination of aspirin plus fish oil therapy caused a significantly greater inhibition of adenosine diphosphate and collagen‐induced whole blood aggregometry compared to aspirin alone. Clinical Significance: Fish oil added to aspirin therapy appears to augment inhibition of some measures of platelet function in healthy dogs. [ABSTRACT FROM AUTHOR]

Summary: Although omalizumab (anti‐IgE) is currently only approved for the treatment of asthma and chronic idiopathic urticaria, it has also been studied as an off‐label treatment for numerous allergic conditions, including use as an adjunct to allergen immunotherapy in the treatment of allergic rhinitis, asthma, venom hypersensitivity and food allergy. We conducted a review of publications involving the use of omalizumab with allergen immunotherapy, by searching PubMed with key search terms of “omalizumab” and “immunotherapy.” Omalizumab has been used in combination with inhalant allergen immunotherapy for the treatment of seasonal allergic rhinitis and comorbid asthma. While there have been no randomized controlled trials evaluating the addition of omalizumab to venom IT, several case reports and small patient series have been published on the use of omalizumab with venom IT. Omalizumab has been used in conjunction with oral immunotherapy for the treatment of milk, peanut and egg, as well as other foods in multi‐allergen protocols. In conclusion, omalizumab used in conjunction with immunotherapy has shown promising results, especially in the reduction of adverse reactions. At this stage, larger, randomized, placebo‐controlled trials are needed to better identify those patients who would benefit the most from the addition of omalizumab to immunotherapy, as well as optimal dosing strategies and duration of treatment. [ABSTRACT FROM AUTHOR]

Background: Positive antinuclear antibody and direct antiglobulin tests support diagnoses such as systemic lupus erythematosus and immune‐mediated anemia, respectively. Positive tests may occur in cats, but the prevalence of positive results in healthy cats is not well known. Objective: The study's purpose was to determine prevalences of positive antinuclear antibody and direct antiglobulin tests in healthy cats. Methods: Antinuclear antibody titers were measured by indirect immunofluorescence, and anti‐erythrocyte antibodies were measured by the microtitration direct antiglobulin test at 37, 23, and 4°C in 61 client‐owned and 28 facility‐owned cats. Differences between the 2 groups were examined using chi‐squared tests. Results: For the antinuclear antibody tests, 70% of client‐owned cats were negative, 10% had weak titers (1:40–1:80), and 20% had strong titers (1:160–1:320). Facility‐owned cats had significantly fewer positive titers with 96% negative and one positive (1:8). For the antiglobulin test at 37°C, 93% of all cats were negative, 2 cats in each group were positive at low dilutions (1:2), and 2 client‐owned cats were transiently positive at high dilutions (≥ 1:2048). At 23°C, 90% of all cats were negative, and 2 client‐owned and 5 facility‐owned cats were positive at low dilutions (1:2–1:8). At 4°C, 67% of client‐owned cats had invalid results (negative control well agglutination), and 33% had negative results, while of facility‐owned cats 14% had invalid results, 14% had agglutination at low dilutions, and 72% were negative. Conclusion: Healthy cats may have positive antinuclear antibody and direct antiglobulin tests, but the prevalence of strong reactions is low. [ABSTRACT FROM AUTHOR]

Background: Platelet function testing may be warranted to assess response to aspirin and clopidogrel. Hypothesis/Objectives: To evaluate the effects of aspirin, clopidogrel, or combination therapy using 3 platelet function tests: Multiplate Analyzer (MP), Platelet Function Analyzer‐200 (PFA), and Plateletworks (PW). Animals: Six healthy laboratory Beagles. Methods: Randomized double‐blind placebo‐controlled study (crossover design). Dogs were given aspirin 1 mg/kg, clopidogrel 2 mg/kg, or combination therapy for 1 week each, with a washout period of 2 weeks. Platelet function was assessed on days 0 and 7 of each phase using MP (adenosine diphosphate [ADP], arachidonic acid [AA], collagen [COL] agonists), PFA (P2Y, COL‐ADP [CADP], COL‐Epinephrine [CEPI] cartridges), and PW (ADP, AA, COL agonists). Platelet counts were obtained with impedance and optical counters. Results: For MP, mean aggregation was decreased for COL and AA with combination therapy and for ADP with all treatments. For PFA, mean CT was increased for the CEPI cartridge with aspirin; and for the P2Y and CADP cartridges with clopidogrel or combination therapy. More dogs receiving clopidogrel showed an increase in PFA CT using the P2Y than the CADP cartridge. For PW, mean aggregation was decreased for AA with all treatments; for ADP with clopidogrel or combination therapy; and for COL with clopidogrel. The PW results with the 2 hematology counters showed almost perfect agreement. Conclusion and Clinical Importance: All platelet function tests detected treatment effects in some dogs and may have utility for monitoring therapy. [ABSTRACT FROM AUTHOR]

Background Colonic dilation is common in children with intractable functional constipation ( FC). Our aim was to describe the association between segmental colonic dilation and colonic dysmotility in children with FC. Methods We performed a retrospective study on 30 children with intractable FC (according to the Rome III criteria) who had undergone colonic manometry and contrast enema within a 12-month time period. Colonic diameter was measured at 5 cm intervals from the anal verge up to the splenic flexure. Moreover, the distance between the lateral margins of the pedicles of vertebra L2 was measured to provide a ratio (colonic diameter or length/distance between the lateral margins; 'standardized colon size' [ SCS]). All manometry recordings were visually inspected for the presence of high-amplitude propagating contractions ( HAPCs); a parameter for colonic motility integrity. The intracolonic location of the manometry catheter sensors was assessed using an abdominal X-ray. Key Results Colonic segments with HAPCs had a significantly smaller median diameter than colonic segments without HAPCs (4.08 cm vs 5.48 cm, P<.001; SCS 1.14 vs 1.66, P=.001). Children with prematurely terminating HAPCs had significantly larger SCS ratios for colonic diameter than children with fully propagating HAPCs ( P=.008). SCS ratios for the length of the rectosigmoid and the descending colon and the SCS ratio for sigmoid colon diameter were significantly larger in children with FC compared to a previously described normative population ( P<.0001, P<.0001 and P=.0007 respectively). Conclusions & Inferences Segmental colonic dilation was associated with prematurely terminating HAPCs and may be a useful indicator of colonic dysmotility. [ABSTRACT FROM AUTHOR]

A growing number of detailed geochemical studies of Ediacaran (635-541 Ma) marine successions have provided snapshots into the redox environments that played host to the earliest known metazoans. Whilst previous compilations have focused on the global evolution of Ediacaran water column redox chemistry, the inherent heterogeneity evident in palaeogeographically distinct environments demands a more dissected approach to better understand the nature, interactions and evolution of extrinsic controls on the development of early macrobenthic ecosystems. Here, we review available data of local-scale redox conditions within a palaeogeographic and sequence stratigraphic framework, to explore the mechanisms controlling water column redox conditions and their potential impact on the record of metazoans. The openly connected Laurentian margin, North America (632-540 Ma) and Nama basin, Namibia (550-538 Ma), and the variably restricted Yangtze Block, South China (635-520 Ma), show continued redox instability after the first fossil evidence for metazoans. This may support opportunistic benthic colonisation during periods of transient oxygenation amidst episodic upwelling of anoxic waters beneath a very shallow, fluctuating chemocline. The first skeletal metazoans appeared under conditions of continued redox stratification, such as those which characterise the Dengying Formation of the Yangtze Block and the Kuibis Subgroup of the Nama basin. Current data, however, suggests that successful metazoan reef-building demanded more persistent oxia. We propose that cratonic positioning and migration throughout the Ediacaran Period, in combination with gradually increasing dissolved oxygen loading, may have provided a first-order control on redox evolution through regulating circulation mechanisms in the Mirovian Ocean. Some unrestricted lower slope environments from mid-high latitudes benefited from sustained oxygenation via downwelling, whilst transit of isolated cratons towards more equatorial positions stifled pervasive ventilation either through ineffective surface ocean mixing, Ekman-induced upwelling, elevated surface ocean productivity or a combination of these processes. [ABSTRACT FROM AUTHOR]

Mesolithic human remains are rare in the archaeological record of the French Mediterranean. Only the island of Corsica has so far produced relatively well-preserved burials, and recent archaeological excavations have brought to light new Mesolithic human remains. The site of Campu Stefanu, located in Sollacaro in the southeast of the island, contained a collective burial of seven to eight individuals in a previously unobserved funerary context. A re-evaluation of collections in regional museums yielded the remains from another Mesolithic individual from the site of Torre d' Aquila, excavated at Pietracorbara, in the northern part of the island, at the beginning of the 1990s. These two discoveries presented the rare opportunity to obtain new radiocarbon dates and paleodietary insights from this crucial time period using stable isotope analysis (δ13C, δ15N) on collagen. From Campu Stefanu, one individual had sufficient collagen preserved for radiocarbon dating, revealing that it is the oldest Mesolithic human known on the island, dated to 10216-9920 cal. BP. At Torre d'Aquila, radiocarbon dates indicate that the individual belonged to a younger Mesolithic phase than Campu Stefanu, dated to 9903-9596 cal. BP. δ13C and δ15N isotope ratios are similar between the Campu Stefanu and Torre d'Aquila individuals and indicate a diet dominated by the consumption of terrestrial animal protein and a lack of marine resources. These findings are in contrast with the previous results from two other Mesolithic individuals from Corsica from the sites of Araguina Sennola and Monte Leone, for which about 25-30% of the consumed proteins came from a marine diet. The dietary variability recorded in Corsica is consistent with results obtained from Mesolithic human remains of Sicily and the Iberian Peninsula. We can hypothesise, that despite the nomadic lifestyle, the distance to the sea played a major role in Mesolithic food choices in Corsica. Copyright © 2016 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

While carbonate reservoirs are recognized to be weakly-to-moderately oil-wet at the core-scale, pore-scale wettability distributions remain poorly understood. In particular, the wetting state of micropores (pores <5 µm in radius) is crucial for assessing multiphase flow processes, as microporosity can determine overall pore-space connectivity. While oil-wet micropores are plausible, it is unclear how this may have occurred without invoking excessively high capillary pressures. Here we develop a novel mechanistic wettability alteration scenario that evolves during primary drainage, involving the release of small polar non-hydrocarbon compounds from the oil-phase into the water-phase. We implement a diffusion/adsorption model for these compounds that triggers a wettability alteration from initially water-wet to intermediate-wet conditions. This mechanism is incorporated in a quasi-static pore-network model to which we add a notional time-dependency of the quasi-static invasion percolation mechanism. The model qualitatively reproduces experimental observations where an early rapid wettability alteration involving these small polar species occurred during primary drainage. Interestingly, we could invoke clear differences in the primary drainage patterns by varying both the extent of wettability alteration and the balance between the processes of oil invasion and wetting change. Combined, these parameters dictate the initial water saturation for waterflooding. Indeed, under conditions where oil invasion is slow compared to a fast and relatively strong wetting change, the model results in significant non-zero water saturations. However, for relatively fast oil invasion or small wetting changes, the model allows higher oil saturations at fixed maximum capillary pressures, and invasion of micropores at moderate capillary pressures. [ABSTRACT FROM AUTHOR]

Namacalathus hermanastes is one of the oldest known skeletal metazoans, found in carbonate settings of the terminal Ediacaran (~550-541 million years ago [Ma]). The palaeoecology of this widespread, goblet-shaped, benthic organism is poorly constrained yet critical for understanding the dynamics of the earliest metazoan communities. Analysis of in situ assemblages from the Nama Group, Namibia (~548-541 Ma), shows that Namacalathus exhibited size variation in response to differing water depths, hydrodynamic conditions and substrate types. In low-energy, inner ramp environments, Namacalathus attains the largest average sizes but grew in transient, loosely aggregating, monospecific aggregations attached to microbial mats. In high-energy mid-ramp reefs, Namacalathus spatially segregated into different palaeoecological habitats with distinct size distributions. In outer ramp environments, individuals were small and formed patchy, dense, monospecific aggregations attached to thin microbial mats. Asexual budding is common in all settings. We infer that variations in size distribution in Namacalathus reflect differences in habitat heterogeneity and stability, including the longevity of mechanically stable substrates and oxic conditions. In the Nama Group, long-lived skeletal metazoan communities developed within topographically heterogeneous mid-ramp reefs, which provided diverse mechanically stable microbial substrates in persistently oxic waters, while inner and outer ramp communities were often ephemeral, developing during fleeting episodes of either oxia and/or substrate stability. We conclude that Namacalathus, which forms a component of these communities in the Nama Group, was a generalist that adapted to various palaeoecological habitats within a heterogeneous ecosystem landscape where favourable conditions persisted, and was also able to opportunistically colonise transiently hospitable environments. These early skeletal metazoans colonised previously unoccupied substrates in thrombolitic reefs and other microbial carbonate settings, and while they experienced relatively low levels of interspecific competition, they were nonetheless adapted to the diverse environments and highly dynamic redox conditions present in the terminal Ediacaran. [ABSTRACT FROM AUTHOR]

Background In a previously reported Co FAR study, 55 subjects with egg allergy underwent randomized, placebo-controlled egg oral immunotherapy ( eOIT). Active treatment induced desensitization in most and sustained unresponsiveness ( SU) in a smaller subset. We hypothesized that component-resolved analysis of IgE, IgG4, IgA, IgA1, and IgA2 may identify potential biomarkers of SU in OIT subjects. Methods Longitudinal samples for 51 egg-allergic subjects (37 active and 14 placebo) were available. Egg white ( EW)-, ovalbumin ( OVA)-, and ovomucoid ( OVM)-specific levels of IgA, IgA1, and IgA2 were quantified by ELISA. IgE and IgG4 to these antigens were quantified using Immuno CAP ®. Clinical responders achieved SU to egg; all others were considered nonresponders. Between-group comparisons were made among active and placebo, as well as responders and nonresponders. Results No placebo subjects achieved responder status. Through month 48, among the 37 active subjects, baseline IgE- OVM was lower in responders (median 3.97 kU/l, n = 19) than in nonresponders (10.9 kU/l, n = 18, P = 0.010). Logistic regression analysis revealed that lower baseline IgE- EW ( P = 0.038), IgE- OVM ( P = 0.032), and a higher IgG4/IgE- OVM ratio ( P = 0.013) were associated with clinical response. Relative increases in IgG4- EW, IgA- EW, and IgA2- EW were observed in responders ( P = 0.024, 0.024, and 0.029, respectively). IgG4/IgE, IgA/IgE, and IgA2/IgE ratios for EW and IgA/IgE ratio for OVA were found to be significantly elevated among responders ( P = 0.004, 0.009, 0.028, and 0.008, respectively). Conclusions Increased IgG4- EW, IgA- EW, and IgA2- EW during eOIT are associated with clinical response to eOIT. Lower pretreatment IgE- EW and IgE- OVM are also associated with SU. Future studies are needed to evaluate and validate these potential biomarkers. [ABSTRACT FROM AUTHOR]

Oxytocin ( OT) is a neuromodulator that facilitates pair-bonding, maternal care and social approach. OT is considered to promote these social behaviours by enhancing the salience and reinforcing effects of relevant social stimuli. There is the additional possibility that OT per se may be rewarding. To test this, we investigated whether female rats would voluntarily self-administer OT. Female Long-Evans rats were ovariectomised and then received an oestrogen implant and an i.c.v. cannula. Rats were tested in an operant chamber with active and inactive levers. They were initially tested for 4 h/day on a fixed-ratio 5 schedule for self-administration of artificial cerebral spinal fluid ( aCSF) for 5 days, followed by aCSF, or OT, at 1 or 10 ng/μl for another 5 days. Rats self-administering aCSF made 36.2 ± 6.2 active lever responses/4 h versus 14.9 ± 3.4 inactive responses. Responses for 1 ng/μl OT were similar. However, rats self-administering 10 ng/μl OT made significantly more active lever responses (67.8 ± 12.0 per 4 h), and received 121.4 ± 21.0 ng OT/4 h. To determine whether reduced anxiety contributes to the reinforcing effects of OT, rats received an infusion of aCSF or OT at 0.3 or 3.0 μg immediately before testing on the elevated plus maze. There was no effect of OT on anxiety as reflected by percentage time spent on the open arms, as well as no effect of OT on locomotion as measured either by the number of closed arm entries or the number of total arm entries. These results suggest that OT may be rewarding, and that this is not a result of the anxiolytic effects of OT. [ABSTRACT FROM AUTHOR]

The article examines two cases of R406W microtubule-associated protein tau (MAPT) mutation in Great Britain. Topics covered include analysis of the case of a 66-year-old woman with slow progressive change in personality and the case of a 64-year-old woman with behavioral changes associated with confabulation and predominant right temporal lobe atrophy in both patients.

Summary: Background: Obese children are typically less physically active than their normal‐weight peers and are often assumed to be ‘unfit’. Objective: Investigate the relationships between adiposity, physical activity levels and cardiorespiratory fitness (CRF) in obese and normal‐weight children. A secondary aim was to examine obese/normal‐weight differences in CRF. Methods: Obese (N = 107) and normal‐weight (N = 132) 10–13‐year‐olds participated. Fat‐free mass (FFM), percent fat, physical activity and peak oxygen uptake (VO2peak) were assessed. Analyses were adjusted for socioeconomic status (SES). Results: Higher percent fat was inversely associated with VO2peak normalized for mass (r = −0.780, P < 0.001) even after controlling for physical activity (r = −0.673, P < 0.001). While higher percent fat was also inversely associated with VO2peak normalized for FFM, this was only significant in males (r = −0.247, P = 0.004) and did not persist after controlling for physical activity (r = −0.059 P = 0.526). Compared with normal‐weight children, obese children had higher absolute VO2peak, lower VO2peak corrected for mass (P ≤ 0.009) and lower VO2peak corrected for FFM (P = 0.041) that did not persist after controlling for SES (P = 0.086). Conclusion: Obesity‐related inefficiencies in CRF were evident. Higher adiposity was associated with poorer CRF relative to mass, irrespective of physical activity levels. However, low physical activity levels may be responsible for associations between adiposity and CRF relative to FFM seen in boys, indicating the importance of encouraging physical activity. [ABSTRACT FROM AUTHOR]

Background: Platelet function tests are influenced by biologic variability, including inter-individual (CVG) and intra-individual (CVI), as well as analytic (CVA) variability. Variability in canine platelet function testing is unknown, but if excessive, would make it difficult to interpret serial results. Additionally, the correlation between platelet function tests is poor in people, but not well described in dogs. Objectives: The aims were to: (1) identify the effect of variation in preanalytic factors (venipuncture, elapsed time until analysis) on platelet function tests; (2) calculate analytic and biologic variability of adenosine diphosphate (ADP) and arachidonic acid (AA)-induced thromboelastograph platelet mapping (TEG-PM), ADP-, AA-, and collagen-induced whole blood platelet aggregometry (WBA), and collagen/ADP and collagen/epinephrine platelet function analysis (PFA-CADP, PFA-CEPI); and (3) determine the correlation between these variables. Methods: In this prospective observational trial, platelet function was measured once every 7 days, for 4 consecutive weeks, in 9 healthy dogs. In addition, CBC, TEG-PM, WBA, and PFA were performed. Results: Overall coefficients of variability ranged from 13.3% to 87.8% for the platelet function tests. Biologic variability was highest for AA-induced maximum amplitude generated during TEG-PM (MAAA; CVG = 95.3%, CVI = 60.8%). Use of population-based reference intervals (RI) was determined appropriate only for PFA-CADP (index of individuality = 10.7). There was poor correlation between most platelet function tests. Conclusions: Use of population-based RI appears inappropriate for most platelet function tests, and tests poorly correlate with one another. Future studies on biologic variability and correlation of platelet function tests should be performed in dogs with platelet dysfunction and those treated with antiplatelet therapy. [ABSTRACT FROM AUTHOR]

We describe new, ecologically complex reef types from the Ediacaran Nama Group, Namibia, dated at ~548 million years ago (Ma), where the earliest known skeletal metazoans, Cloudina riemkeae and Namacalathus, formed extensive reefs up to 20 m in height and width. C. riemkeae formed densely aggregating assemblages associated with microbialite and thrombolite, each from 30 to 100 mm high, which successively colonised former generations to create stacked laminar or columnar reef frameworks. C. riemkeae individuals show budding, multiple, radiating attachment sites and cementation between individuals. Isolated Namacalathus either intergrew with C. riemkeae or formed dense, monospecific aggregations succeeding C. riemkeae frameworks, providing a potential example of environmentally mediated ecological succession. Cloudina and Namacalathus also grow cryptically, either as pendent aggregations from laminar crypt ceilings in microbial framework reefs or as clusters associated with thrombolite attached to neptunian dyke walls. These reefs are notable for their size, exceeding that of the succeeding Lower Cambrian archaeocyath-microbial communities. The repeated colonisation shown by C. riemkeae of former assemblages implies philopatric larval aggregation to colonise limited favourable substrates. As such, not only were skeletal metazoans more important contributors to reef building in the Ediacaran, but there were also more variable reef types with more complex ecologies, than previously thought. Such an abundance of inferred suspension feeders with biomineralised skeletons indicates the efficient exploitation of new resources, more active carbon removal with a strengthened energy flow between planktic and benthic realms, and the rise of biological control over benthic carbonate production. These mark the prelude to the Cambrian Explosion and the modernisation of the global carbon cycle. [ABSTRACT FROM AUTHOR]