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We study whether psychological responses to trauma are associated with financial risk‐taking behavior. Posttraumatic stress disorder (PTSD) symptoms for individuals, assessed after the traumatic experiences, are used as psychological responses to the events. Individuals who experience moderate‐level PTSD symptoms are 1.5% more likely to invest in risky assets, whereas individuals with high‐level PTSD symptoms are 2.4% less likely to invest in risky assets. Further analysis suggests that the association between PTSD symptoms and risk‐taking comes through a preferences channel rather than a beliefs channel. [ABSTRACT FROM AUTHOR]

ABSTRACT Background Aims Methods Results Conclusions Perianal fistulizing Crohn's disease (PFCD) is a challenging and debilitating phenotype of Crohn's disease that can negatively affect quality of life. Studies have begun to uncover the physiologic mechanisms involved in wound repair as it relates to PFCD and how aberrations in these mechanisms may contribute to fistula persistence.To review the physiologic and pathophysiologic mechanisms of wound repair in PFCD and how specific therapeutic strategies may impact their outcomes.We reviewed the latest published literature on wound repair as it relates to PFCD.Wound repair can be categorised into three overlapping biological phases: localised inflammation, cell recruitment/proliferation and tissue remodelling. Each is tightly regulated since insufficient or excessive activation can result in, respectively, chronic wounds and fibrotic tissue, both of which can impair organ function. In PFCD, the outcomes of wound repair include restitution (complete healing), epithelialisation and chronic wounds. Treatment of PFCD should take into consideration the distinct phases of wound repair. Therefore, the ability to differentiate between each phase of wound repair and their outcomes may help physicians deliver the most effective treatment strategy at the most appropriate time.This review provides a comprehensive overview of the phases of wound repair and specific treatment strategies for each to provide clinicians with a rational framework for managing PFCD. [ABSTRACT FROM AUTHOR]

Smart antibacterial coatings with surface‐independent methods, on‐demand antibiotic release, and real‐time drug loading monitoring capabilities have attracted increasing interest in the fields of medical devices, antibiotics delivery platforms, and implantable devices. Addressing the complexity inherent in existing methods, this work innovates by simplifying the synthesis process and integrating aminoglycosides with metallosupramolecules to develop versatile and effective coatings. These coatings not only exhibit exceptional biological activity and efficient antibacterial properties both in vitro and in vivo, but also enable high drug loading and controlled release. Significantly, their application across various devices has demonstrated profound therapeutic effects in treating implant infections and promoting bacterial wound healing. A key advancement of these coatings is the integration of color‐changing indicators for real‐time monitoring of drug levels, enhancing the precision of wound care, and facilitating timely clinical interventions. This research marks a significant stride in the development of more accessible, bioactive, and smart antibacterial materials, opening new avenues in the field of smart medical coatings. [ABSTRACT FROM AUTHOR]

The challenge in treating diabetic foot infections caused by drug‐resistant bacteria is rapidly eradicating bacteria and accelerating wound healing. NIR‐II‐induced photothermal therapy (PTT) offers notable advantages over conventional treatments, such as broad‐spectrum bactericidal efficacy, better penetration depth in biological tissues, and higher skin tolerance thresholds, making it particularly suitable for addressing diabetic foot infections. Herein, it is found that NIR‐II dye IR26 exhibited good photothermal effects but poor photothermal stability. By modifying IR26 with methoxy and triphenylamine groups, a novel D–A–D‐type NIR‐II small molecule photothermal agent (IRC) is developed, with high photothermal stability and photothermal conversion efficiency (44.3%). IRC exhibited a maximum absorption wavelength of 1166 nm and a maximum emission wavelength of 1238 nm. However, PTT alone cannot effectively promote diabetic wound healing. Therefore, a novel nanoplatforms (Cur‐IRC@PCM) are developed by coloading IRC and curcumin, a natural wound–healing compound, into thermosensitive liposomes to treat MRSA‐infected diabetic wounds. Under 980 nm laser irradiation, Cur‐IRC@PCM provided PTT and controlled the precise release of curcumin, effectively synergizing to eradicate drug‐resistant bacteria and accelerate wound closure. The intelligent antibacterial nanoplatforms Cur‐IRC@PCM also exhibits excellent biocompatibility, rendering it a promising therapeutic tool in biomedical fields for combating drug‐resistant bacterial infections. [ABSTRACT FROM AUTHOR]

The ability to precisely arrange and control the assembly of diverse cell types into intricate 3D structures remains a critical challenge in tissue engineering. Herein, a versatile and programmable 3D cell sheet assembly is described technology by developing a biodegradable nanochannel (BNC) membrane to fulfill this unmet need. This membrane, hierarchically assembled from 2D nanomaterial aggregates, exhibits both exceptional fluid permeability and rapid biodegradation under physiological conditions. The unique properties of the BNC membrane enable precise spatial and temporal control over cell assembly, facilitating the creation of complex 3D cellular architectures. The BNC membrane is integrated with a programmable negative‐pressure‐based cell assembly strategy to form single and multicellular 3D sheets in a highly controllable manner. To demonstrate the feasibility and translatability of this technology in the field of tissue engineering approaches to screen stem cell‐derived therapeutics with "core–shell" macrophage‐fibroblast multicellular patterns and treat murine diabetic skin wounds via scaffold‐free 3D adipose‐derived mesenchymal stem cell (ADMSC) sheets are devised. In summary, the results demonstrate that the BNC membrane‐based 3D cell sheet assembly approach significantly advances current tissue engineering capabilities, offering substantial potential for both regenerative medicine applications and the development of physiologically relevant disease models. [ABSTRACT FROM AUTHOR]

Placenta accreta spectrum (PAS) is a relatively new obstetric condition which, until recently, was poorly understood. The true incidence is unknown because of the poor quality and heterogeneous diagnostic criteria. Classification systems have attempted to provide clarity on how to grade and diagnose PAS, but these are no longer reflective of our current understanding of PAS. This is particularly true for placenta percreta, which referred to extrauterine disease, as recent studies have demonstrated that placental villi associated with PAS have minimal potential to invade beyond the uterine serosa. It is accepted that PAS is a direct consequence of previous iatrogenic uterine injury, most commonly a previous cesarean section. Here, we "look back to look forwards"—starting with the primary predisposing factor for PAS, an iatrogenic uterine injury and subsequent wound healing. We then consider the evolution of definitions and diagnostic criteria of PAS from its first description over a century ago to current classifications. Finally, we discuss why modifications to the current classifications are needed to allow accurate diagnosis of this rare but life‐threatening complication, while avoiding overdiagnosis and potential patient harm. Synopsis: Current clinical grading systems for placenta accreta spectrum do not reflect current understanding of etiology and warrant updating as overdiagnosis is becoming a serious problem. [ABSTRACT FROM AUTHOR]

Aberrant activation of dermal fibroblasts during wound healing often leads to debilitating fibrotic changes in the skin, such as scleroderma and keloids. However, the underlying cellular and molecular mechanisms remain elusive. Here, we established a wound‐induced skin fibrosis (WISF) mouse model in mature adult mice, characterised by excessive deposition of collagen bundles, loss of dermal adipocytes, and enrichment of DPP4+Ly6A+THY1+ hypodermal interstitial adipocyte progenitors (HI‐APs) and pericytes, resembling human fibrotic skin diseases. This WISF model exhibited an age‐dependent gain of fibrotic characteristics, contrasting with the wound‐induced hair neogenesis observed in younger mice. Through comprehensive analyses of the WISF, we delineated a trajectory of fibroblast differentiation that originates from HI‐APs. These progenitors highly expressed several extracellular matrix (ECM) genes and exhibited a TGFβ pathway signature. TGFβ was identified as the key signal to inhibit the adipogenic potential and maintain the fibrogenic potential of dermal APs. Additionally, administering a TGFβ receptor inhibitor to wound scar reduced the abundance of ECM‐producing APs. Finally, analysis of human scleroderma skin tissues revealed a negative correlation between the expression of AP‐, ECM‐, and TGFβ pathway‐related genes and PPARG. Overall, this study establishes a wound‐induced skin fibrosis mouse model and demonstrates that TGFβ‐mediated blockage of HI‐AP differentiation is crucial for driving fibrotic pathology. Targeting HI‐APs and adipogenesis may provide novel avenues for developing disease‐modifying therapies for fibrotic skin diseases. [ABSTRACT FROM AUTHOR]

Objective: Diabetic foot ulcers (DFU) are one of the most destructive complications of diabetes mellitus. The aim of this study was to link miR‐155 and SOX2 with DFU to explore the regulation of wound healing by DFU and its potential mechanism. Methods: Human keratinocytes (HaCaT) were induced with advanced glycation end products (AGEs) to construct DFU models in vitro. AGE‐induced HaCaT cells were subjected to CCK‐8 assays, flow cytometry, and wound healing assays to evaluate cell proliferation, apoptosis, and migration capacity, respectively. RT–qPCR and Western blotting were used to determine gene and protein expression levels, respectively. N6‐methyladenosine (M6A) levels in total RNA were assessed using an M6A methylation quantification kit. Results: Our results suggested that the inhibition of miR‐155 promoted wound healing in an in vitro DFU model, while the knockdown of HIF‐1α reversed this process, and that HIF‐1α was a target protein of miR‐155. In addition, knockdown of HIF‐1α promoted the m6A level of SOX2 mRNA, inhibited the expression of SOX2, and inhibited the activation of the EGFR/MEK/ERK signaling pathway, thus inhibiting the proliferation and migration of HaCaT cells and promoting the apoptosis of HaCaT cells, while overexpression of SOX2 reversed this effect. We also found that METTL3 knockdown had the opposite effect of HIF‐1α knockdown. Conclusions: Inhibition of miR‐155 promoted the expression of HIF‐1α and attenuated the m6A modification of SOX2 mRNA, thereby promoting the expression of SOX2 and activating the downstream EGFR/MEK/ERK signaling pathway to promote wound healing in an in vitro DFU model. [ABSTRACT FROM AUTHOR]

Introduction: Pharyngocutaneous fistula (PCF) is one of the most challenging complications observed after a total laryngectomy. Since the biochemical components of platelet‐rich fibrin (PRF) have well‐known synergistic effects on the healing processes, this study aimed to demonstrate the contribution of PRF application to pharyngeal healing in patients undergoing a total laryngectomy for laryngeal cancer. Methods: The study compared patients who underwent a total laryngectomy due to laryngeal squamous cell carcinoma and had a PRF membrane placed during the pharyngoesophageal closure with those who did not. There were two groups: PRF‐positive and PRF‐negative. In the PRF‐positive group, after the completion of the total laryngectomy and moving on to the pharyngoesophageal closure stage, along the suture line, PRF material is laid in two pieces in a T‐shape and secured with several sutures. No PRF application was done in the PRF‐negative group. Pharyngeal healing steps (nasogastric feeding, oral feeding, development of a fistula), haemoglobin and albumin values, tumour involvement areas, time to oral intake and length of hospital stay were recorded for all patients. Results: This study reviewed the records of 33 patients who underwent pharyngoesophageal closure with PRF application after a total laryngectomy (PRF‐positive group) and 35 patients without PRF application (PRF‐negative group). When comparing patients in terms of developing a PCF, 6% (n = 2) of patients in the PRF‐positive group and 25.7% (n = 9) in the PRF‐negative group developed a fistula. This ratio was significantly higher in the PRF‐negative group (p = 0.027). Conclusion: The application of PRF in pharyngoesophageal reconstruction after a total laryngectomy may strengthen wound healing and reduce the risk of PCF development. Trial Registration: This study is a retrospective designed study; therefore, there is no clinical trial registration [ABSTRACT FROM AUTHOR]

The dilemma of diabetic infectious wound healing lies in inhibiting the pathogenic colonization and regulating the hyperglycemia. The pivotal anti‐pathogenic efficiency is focused on the confined and gradually weaken reactive oxygen species (ROS) yield in the peculiar micromilieu owing to the materials transformation/dissolution. There, the type‐transformational bio‐heterojunction enzyme (BioHJzyme) with operando composition‐modulation is proposed, which is consisted by glucose oxidase (GOx) decorated type‐I FeSe2/Cu2O heterojunction. It exhibits robust catalytic efficiency to produce ROS. Followed, the type‐I heterojunction can be transformed into dual Z‐scheme heterojunction with a composition of FeSe2/CuSe/Cu2O primed by the infectious acid micromilieu with a meliorative energy band. It is conducive to the NIR‐induced capabilities to improve ROS yield. The GOx can consume glucose to produce H2O2 as an amplifier. The combined catalytic efficiency of type‐I heterojunction and the followed enhanced NIR‐induced capabilities of dual Z‐scheme heterojunction can powerfully remove the pathogenic colonization. On top of that, the sample can downgrade the Forkhead box O (FoxO) signaling pathway to regulate cell cycle, facilitate cell proliferation, and further promote wound repairment. This work provides an effective and feasible antibacterial strategy and way of thinking for the diabetic infectious wound healing. [ABSTRACT FROM AUTHOR]

Type 2 diabetes mellitus (T2DM) is not just a local health issue but a significant global health burden, affecting patient outcomes and clinical management worldwide. Despite the wealth of studies reporting T2DM biomarkers, there is an urgent need for a comparative review. This review aims to provide a comprehensive analysis based on the reported T2DM biomarkers and how these are linked with other conditions, such as inflammation and wound healing. A comparative review was conducted on 24 001 study participants, including 10 024 T2DM patients and 13 977 controls (CTL; age 30–90 years). Four main profiles were extracted and analysed from the clinical reports over the past 11 years: haematological (1084 cases vs. 1458 CTL), protein (6753 cases vs. 9613 CTL), cytokine (975 cases vs. 1350 CTL) and lipid (1212 cases vs. 1556 CTL). This review provides a detailed analysis of the haematological profile in T2DM patients, highlighting fundamental changes such as increased white blood cells and platelet counts, accompanied by decreases in red blood cell counts and iron absorption. In the serum protein profile, a reduction in albumin and anti‐inflammatory cytokines was noted along with an increase in globulin levels and pro‐inflammatory cytokines. Furthermore, changes in lipid profiles were discussed, specifically the decreases in high‐density lipoprotein (HDL) and the increases in low‐density lipoprotein (LDL) and triglycerides. Understanding the changes in these four biomarker profiles is essential for developing innovative strategies to create diagnostic and prognostic tools for diabetes management. [ABSTRACT FROM AUTHOR]

Aim: This prospective study investigated the salivary proteome before and after periodontal therapy. Materials and Methods: Ten systemically healthy, non‐smoking, stage III, grade C periodontitis patients underwent non‐surgical periodontal treatment. Full‐mouth periodontal parameters were measured, and saliva (n = 30) collected pre‐ (T0), and one (T1) and six (T6) months post‐treatment. The proteome was investigated by label‐free quantitative proteomics. Protein expression changes were modelled over time, with significant protein regulation considered at false discovery rate <0.05. Results: Treatment significantly reduced bleeding scores, percentages of sites with pocket depth ≥5 mm, plaque and gingival indexes. One thousand seven hundred and thirteen proteins were identified and 838 proteins (human = 757, bacterial = 81) quantified (≥2 peptides). At T1, 80 (T1 vs. T0: 60↑:20↓), and at T6, 118 human proteins (T6 vs. T0: 67↑:51↓) were regulated. The salivary proteome at T6 versus T1 remained stable. Highest protein activity post‐ versus pre‐treatment was observed for cellular movement and inflammatory response. The small proline‐rich protein 3 (T1 vs. T0: 5.4‐fold↑) and lymphocyte‐specific protein 1 (T6 vs. T0: 4.6‐fold↓) were the top regulated human proteins. Proteins from Neisseria mucosa and Treponema socranskii (T1 vs. T0: 8.0‐fold↓, 4.9‐fold↓) were down‐regulated. Conclusions: Periodontal treatment reduced clinical disease parameters and these changes were reflected in the salivary proteome. This underscores the potential of utilizing saliva biomarkers as prognostic tools for monitoring treatment outcomes. [ABSTRACT FROM AUTHOR]

Aim: To assess the Doppler ultrasonographic tissue perfusion at dental implant sites augmented with connective tissue graft (CTG) using coronally advanced flap (CAF) or tunnel technique (TUN). Materials and Methods: Twenty‐eight patients presenting with isolated healthy peri‐implant soft‐tissue dehiscence (PSTD) were included in this randomized clinical trial. PSTDs were treated with either CAF + CTG or TUN + CTG. Ultrasound scans were taken at baseline, 1 week, 1 month, 6 months and 12 months. Tissue perfusion at the mid‐facial, mesial and distal aspects of the implant sites was assessed by colour Doppler velocity (CDV) and power Doppler imaging (PDI). Early vascularization of the graft and the flap at 1 week and at 1 month were evaluated via dynamic tissue perfusion measurements (DTPMs), including flow intensity (FI), mean perfusion relief intensity (pRI) and mean perfused area (pA). Results: Regression analysis did not reveal significant differences in terms of mid‐facial CDV and PDI changes between CAF and TUN over 12 months (p >.05), while significant differences between the two groups were observed at the interproximal areas (p <.001 for both CDV and PDI changes). Higher early DTPMs were observed at the TUN‐treated sites in terms of mean FI of the graft (p =.027) and mean FI (p =.024) and pRI of the flap (p =.031) compared with CAF‐treated sites at 1 week. Assessment of the FI direction showed that CTG perfusion at 1 week and at 1 month mainly occurred from the flap towards the implant/bone. Early tissue perfusion outcomes were found to be associated with the 12‐month mean PSTD coverage and mucosal thickness gain. Conclusions: Doppler ultrasonography shows tissue perfusion changes occurring at implant sites augmented with CTG. The main differences in tissue perfusion between CAF and TUN were observed at the interproximal sites, with early perfusion associated with clinical and volumetric outcomes at 12 months. [ABSTRACT FROM AUTHOR]

Upon injury, regenerating skin is metabolically active and requires oxygen for physiological processes related to wound healing. Such processes can be halted in hypoxic conditions common in chronic wounds. Microneedle arrays (MNAs) have been demonstrated to improve therapeutic delivery and wound healing. Recently, few studies have explored the use of oxygen‐releasing MNAs; however, they involve complex manufacturing and handling and fail to eliminate cytotoxic byproducts. To address these challenges, biodegradable and mechanically robust gelatin methacryloyl‐based MNAs are developed that can penetrate the tissue and release oxygen upon exposure to interstitial fluid and wound exudates. The oxygen release rate and biocompatibility of the developed MNAs with different compositions are evaluated and optimized. Interestingly, in vitro studies demonstrate that the optimized compositions can release oxygen at therapeutic levels and significantly increase viability of chronically hypoxic cells to match that of normoxic cells. In vivo studies further confirm that the optimized oxygen‐generating MNAs do not cause any harm or impair healing in a murine model of acute skin injury. Additionally, transcriptomic analysis reveals upregulation of key pathways related to fibroblast motility, lipid metabolism, and a marked reduction in inflammatory signaling, all of which contribute to improved wound healing. The developed strategy can introduce new opportunities in elimination of hypoxia and therefore treatment of chronic wounds. [ABSTRACT FROM AUTHOR]

Chitosan and collagen are two valuable biopolymers for the synthesis and design of biomaterials. In this work, we evaluate the effect of the concentration of chitosan on the physicochemical and biological properties of polyurethane‐crosslinked collagen hydrogels. Chitosan was added with a proportion ranging from 0 to 40 wt%. Depending on the aminoglycoside content, semi‐interpenetrating polymeric networks (semi‐IPNs) or completely IPNs were generated; this is attributed to the occlusion of the chitosan granules in the reticulated fibrillar matrix. The presence of chitosan in these hydrogels provides antibacterial capacity against pathogenic microorganisms such as Escherichia coli, which is corroborated by inhibition halo experiments. Hydrogels with 40 wt% of chitosan only showed improvement in the gelation time and mechanical performance, indicating a dependent relation of these properties with respect to the aminopolysaccharide content. Hydrogels with 10 wt% of chitosan showed the best biomedical performance. They also have the highest reticulation, swelling capability and chemical stability against degradation events such as hydrolysis and proteolysis. Further, they have the best biocompatibility for human monocytes, and they stimulate the secretion of monocyte chemotactic protein‐1 and tumor necrosis factor‐alpha. Also, these hydrogels showed a controlled release of ketorolac at pH 7 and 37 °C. The results of these in vitro biological tests demonstrate the potential application of novel biopolymer‐based hydrogels in chronic wound healing due to their antibacterial capacity and modulation of the biological response of specialized cells in dermal repair treatments, as well as for the design of smart materials for the controlled release of drugs. © 2023 Society of Industrial Chemistry. [ABSTRACT FROM AUTHOR]

Objective: Animal models of vocal fold (VF) surgical injury and scar formation provide insight into the wound healing process. The purpose of this study was to establish an alternative model of surgical injury to the mouse VF using materials commonly available in most research laboratories or for purchase and to investigate wound healing of the epithelium (EP) and lamina propria (LP). Methods: Mice were anesthetized by isoflurane gas delivery and positioned on a platform so that the larynx could be observed using a laryngoscope and dissection microscope. Unilateral VF injury was created using a wire brush. Mice were euthanized and the larynx evaluated 1‐, 3‐, 5‐, 7‐, 14‐, and 28‐days following injury. Histological and immunofluorescent analysis was used to evaluate thickness of the EP, LP area, proliferative (Ki67+) and basal cells (p63+) in the EP, and collagen III content in the LP. Results: The depth of injury reached the superficial thyroarytenoid muscle on Day 1. The thickness of the EP of the injured VF was increased on Days 3 and 5, and the LP area was increased on Days 3, 5, and 7 as compared with the uninjured VF. Ki67+ and p63+ cells were increased on Day 3 and collagen III content was increased on Days 5 and 28 as compared with the uninjured VF. Conclusion: We successfully established an alternative method of creating unilateral VF injury in the mouse. This method will be useful for future research regarding VF surgical injury and wound healing. Level of Evidence: NA Laryngoscope, 135:213–222, 2025 [ABSTRACT FROM AUTHOR]

C‐X‐C motif chemokine ligand 12 (CXCL12; Stromal Cell‐Derived Factor 1 [SDF‐1]), most notably known for its role in embryogenesis and hematopoiesis, has been implicated in tumor pathophysiology and neovascularization. However, its cell‐specific role and mechanism of action have not been well characterized. Previous work by our group has demonstrated that hypoxia‐inducible factor (HIF)‐1 modulates downstream CXCL12 expression following ischemic tissue injury. By utilizing a conditional CXCL12 knockout murine model, we demonstrate that endothelial‐specific deletion of CXCL12 (eKO) modulates ischemic tissue survival, altering tissue repair and tumor progression without affecting embryogenesis and morphogenesis. Loss of endothelial‐specific CXCL12 disrupts critical endothelial–fibroblast crosstalk necessary for stromal growth and vascularization. Using murine parabiosis with novel transcriptomic technologies, we demonstrate that endothelial‐specific CXCL12 signaling results in downstream recruitment of non‐inflammatory circulating cells, defined by neovascularization modulating genes. These findings indicate an essential role for endothelial‐specific CXCL12 expression during the neovascular response in tissue injury and tumor progression. [ABSTRACT FROM AUTHOR]

Chronic wounds, particularly those associated with diabetes, pose a significant clinical challenge due to their tendency to develop biofilms that resist conventional antibiotic treatments. To address this issue, a novel therapeutic strategy utilizing pH‐responsive nanoparticles loaded with aggregation‐induced emission photosensitizers and natural saturated fatty acids (AIE/LA@HMONs−PyB) for effective biofilm penetration and disruption is proposed. Under physiological conditions, AIE/LA@HMONs−PyB are negatively charged. Upon accumulation at infected sites, however, the pyridine betaine group on the surface of AIE/LA@HMONs−PyB enables rapid protonation and charge reversal in the acidic biofilm microenvironment, thereby enhancing their ability to penetrate the biofilm. Upon light irradiation, these nanoparticles generate reactive oxygen species that effectively disrupt the biofilm structure. This process enables the synergistic action of ciprofloxacin at a lower concentration, achieving an exceptional in vitro antibacterial efficiency of 99.99% against methicillin‐resistant Staphylococcus aureus (S. aureus) biofilms. Furthermore, in an in vivo diabetic wound model, this synergistic therapy accelerates wound healing by reducing inflammation, promoting angiogenesis, and enhancing collagen regeneration. The enhanced penetration strategy significantly improves the therapeutic efficacy of this combined approach, offering great promise for advancing chronic wound healing and enhancing patient outcomes. [ABSTRACT FROM AUTHOR]

Background: Trauma and burns are leading causes of death and significant global health concerns. RNA‐binding proteins (RBPs) play a crucial role in post‐transcriptional gene regulation, influencing various biological processes of cellular RNAs. This study aims to review the emerging trends and key areas of research on RBPs in the context of trauma and burns. Methods: A series of relevant articles were manually reviewed, and scientific publications on RBPs related to trauma and burns were retrieved from the Web of Science Core Collection (WoSCC) on May 19, 2024. Bibliometric analysis was performed using R‐bibliometrix, VOSviewer, and CiteSpace. We adhered to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines to conduct the meta‐analysis, followed by a comprehensive review of the selected papers. Results: A total of 539 publications were identified from 2000 to 2024. China was the most productive and collaborative country, with Zhang Y being the most prolific author and PLoS One being the leading publication source. Keyword analysis revealed four distinct clusters. The thematic analysis identified eight key topics, including "RNA‐binding proteins," "traumatic brain injury," and "inflammation." Four studies involving 5.976 patients were included in the final meta‐analysis, which indicated a correlation between RBP expression levels and poor prognosis in traumatic brain injury (TBI). Conclusion: Our findings provide valuable insights into the developing trends and key areas of research on RBPs in trauma and burns. Notably, we identified two primary hotspots: RBPs in the pathophysiological mechanisms of various traumatic injuries and RBPs in the processes of cutaneous wound healing. This rapidly evolving field offers significant reference points for scientific researchers and clinical practitioners. [ABSTRACT FROM AUTHOR]

Disruptions of the eukaryotic plasma membrane due to chemical and mechanical challenges are frequent and detrimental and thus need to be repaired to maintain proper cell function and avoid cell death. However, the cellular mechanisms involved in wound resealing and restoration of homeostasis are diverse and contended. Here, it is shown that clathrin‐mediated endocytosis is induced at later stages of plasma membrane wound repair following the actual resealing of the wound. This compensatory endocytosis occurs near the wound, predominantly at sites of previous early endosome exocytosis which is required in the initial stage of membrane resealing, suggesting a spatio‐temporal co‐ordination of exo‐ and endocytosis during wound repair. Using cytoskeletal alterations and modulations of membrane tension and membrane area, membrane tension is identified as a major regulator of the wounding‐associated exo‐ and endocytic events that mediate efficient wound repair. Thus, membrane tension changes are a universal trigger for plasma membrane wound repair modulating the exocytosis of early endosomes required for resealing and subsequent clathrin‐mediated endocytosis acting at later stages to restore cell homeostasis and function. [ABSTRACT FROM AUTHOR]

ABSTRACT Aims/Introduction Materials and Methods Results Conclusions Diabetic foot ulcer (DFU) is a prevalent complication of diabetes characterized by heightened inflammation and impaired wound‐healing processes. Interleukin‐37 (IL‐37) is a natural suppressor of innate inflammation. Here, we aim to investigate the potential of IL‐37 in enhancing the healing process of diabetic wounds.The skin samples of DFU and non‐diabetic patients during foot and ankle orthopedic surgery were collected. The IL‐37 transgenic mice (IL‐37Tg) were created using CRISPR/Cas‐mediated genome engineering. Mice were administered streptozotocin (STZ, 150 mg/kg) to induce a diabetic model. After 4 weeks, an equidistant full‐thickness excisional wound measuring 8 mm was created on the central back of each mouse and allowed to heal naturally. Body weight and blood glucose levels were measured weekly. The wound area was measured, and skin samples were collected on Day 10 for further Quantitative polymerase chain reaction (qPCR) and WB detection and RNA sequencing analysis.The proinflammation cytokines such as TNF‐α and IL‐1β and the MAPK signaling pathway were significantly increased in the wound margin of DFU patients. Compared with diabetic mice, diabetic IL‐37Tg mice showed a significantly accelerated healing process. The enriched signaling pathways in RNA sequencing included cytokine–cytokine receptor interaction, TNF signaling pathway, and NOD‐like receptor signaling pathway. Through QPCR and WB detection, we found that IL‐37 could inhibit the activated MAPK and NOD‐like signaling pathway, reducing TNF‐α, IL‐1β, and NLRP3 expression in the diabetic wound.IL‐37 promotes skin wound healing in diabetic mice, providing a new possible target for treating diabetic wounds. [ABSTRACT FROM AUTHOR]

The bacterial electron transport chain (ETC) plays a crucial role in biofilm energy metabolism and redox balance, making it a potential target for anti‐biofilm treatment. Herein, mesoporous nanocarbon spheres loaded with Pt nanoparticles (CS‐Pt) are developed to disrupt the bacterial ETC for photo‐enhanced anti‐biofilm. Under near‐infrared laser irradiation, CS‐Pt‐treated biofilms show a positive shift of 0.6 eV in Pt 4f binding energy and a 1.76‐fold increase in anodic current density, demonstrating that the electron loss from CS‐Pt results in an enhanced electron transfer from CS‐Pt to biofilm. Adding the electron quencher NH4Cl to the CS‐Pt‐containing biofilm sample results in reduced biofilm clearance, highlighting the critical role of enhanced electron transfer in effective biofilm eradication. Due to the multifunction of CS‐Pt in inducing hyperthermia, reactive oxgen species generation, and interrupting bacterial ETC, the critical component of ETC, extracellular DNA, in methicillin‐resistant Staphylococcus aureus (MRSA) biofilm is reduced by 89.8%, ultimately leading to a biofilm clearance of 90.3%. Additionally, CS‐Pt achieve 99.5% biofilm eradication and expedites wound healing with a rate of 78.1% in a MRSA‐infected wound model. We present an attractive approach for disintegrating biofilm through the synergistic effect of disrupting ETC, enzyme‐like activity, and phototherapy, offering an innovative perspective on combating biofilm‐associated infections. [ABSTRACT FROM AUTHOR]

Diabetic wound healing presents unique challenges, including impaired angiogenesis, prolonged inflammation, and delayed re‐epithelialization. Advancements in tissue engineering offer promising solutions through cell/drug‐based therapies. Exosomes (Exo) derived from hypoxia‐preconditioned adipose‐derived stem cells (ADSCs) have gained attention for their potential to address these complex issues in diabetic wounds. Existing strategies for Exo delivery aim to overcome drawbacks associated with conventional administration methods, including rapid loss of activity, frequent dosing, and off‐target effects. However, complexities in fabrication, undesirable components within the delivery system, and unforeseen outcomes have hindered the efficacy of these approaches. Thus, an in situ formed hydrogel is engineered using click chemistry to facilitate the convenient encapsulation of hypoxia‐induced Exo. The hydrogel swiftly transitioned into a gel state upon mixing and facilitated the controlled release of Exo at various loading dosages. Through systematic screening of Exo‐hydrogel formulations, it is demonstrated that the encapsulated Exo retained their bioactivity, exhibits therapeutic efficacy in vitro via scratch and tube formation assays. Further, the optimal Exo‐hydrogel promotes accelerated wound healing while preventing scar formation in a diabetic rat wound model. The Exo‐loaded hydrogel represents a promising approach for efficient Exo delivery in wound healing applications and holds potential for broader applications in diverse medical fields. [ABSTRACT FROM AUTHOR]

Aims Methods Results Conclusion The study is to investigate how residual osteomyelitis at conservative surgical resection margins affects the prognosis of diabetic toe osteomyelitis.In this retrospective study, 67 participants with diabetic toe osteomyelitis who underwent conservative surgery were evaluated. The diagnosis of osteomyelitis was based on histopathology, and bone histopathology was done on the resection bone. After discharge, a 16‐week follow‐up was conducted, focusing on the wound healing time as the primary outcome. Cox regression analysis was employed as the primary method to analyse the risk factors that influence wound healing.Among the 67 participants, 48 (71.6%) had positive bone margins, while 19 (28.4%) had negative bone margins. Participants with positive bone margins experienced an average healing time of 60.78 ± 18.50 days, whereas those with negative bone margins had an average healing time of 55.19 ± 14.60 days (p = 0.285). Bone margins (positive vs. negative) did not have an impact on wound healing (HR, 1.195 [95% CI, 0.668–2.136]; p = 0.549). Ankle‐brachial index was identified as a factor influencing wound healing (HR, 5.399 [95% CI, 1.145–25.455]; p = 0.033). There was no statistical difference between the two groups in terms of wound healing rates (40 of 48 [83.3%] vs. 16 of 19 [84.2%], p = 0.93), inpatient days (19.48 ± 5.25 vs. 18.26 ± 4.79 days, p = 0.385) and duration of antibiotics (32.33 ± 5.64 vs. 30.53 ± 6.93 days, p = 0.272).The residual osteomyelitis in diabetic toe osteomyelitis post‐conservative surgery does not impact the wound healing. [ABSTRACT FROM AUTHOR]

Recent advancements in biomedical applications have highlighted the need for nontoxic and organic materials with versatile capabilities. Silver nanoparticles (AgNPs) have emerged as a promising antimicrobial agent due to their exceptional physicochemical properties, whereas hydrogels offer potential applications in biomedicine due to their biocompatibility, biodegradability, and hydrophilicity. AgNPs‐infused hydrogel can offer synergistic approach for various biomedical applications, specifically in wound healing, drug delivery, and antimicrobial coatings. The incorporation of AgNPs into the hydrogel enhances their antimicrobial properties, making them ideal for reducing infections and promoting tissue regeneration. Furthermore, AgNPs‐infused hydrogel can serve as controlled‐release systems for therapeutic agents, which ensures sustained and targeted drug delivery. Most importantly, this type of system offers a potential pathway for overcoming the challenges posed by traditional materials. While AgNPs‐infused hydrogel offer significant advantages for various biomedical applications, challenges such as potential cytotoxicity, environmental concerns, and long‐term effects require further investigation. Overall, this review comprehensively explores the synthesis methods, properties, applications, and challenges associated with AgNPs‐infused hydrogel. [ABSTRACT FROM AUTHOR]

Skin wounds have become an important issue that affects human health and burdens global medical care. Hydrogel materials similar to the natural extracellular matrix (ECM) are one of the best candidates for ideal wound dressings and the most feasible choices for printing inks. Distinct from hydrogels made by traditional technologies, which lack bionic and mechanical properties, 3D printing can promptly and accurately create hydrogels with complex bioactive structures and the potential to promote tissue regeneration and wound healing. Herein, a comprehensive review of multi‐functional 3D printing‐based hydrogel dressings for wound healing is presented. The review first summarizes the 3D printing techniques for wound hydrogel dressings, including photo‐curing, extrusion, inkjet, and laser‐assisted 3D printing. Then, the properties and design approaches of a series of bioinks composed of natural, synthetic, and composite polymers for 3D printing wound hydrogel dressings are described. Thereafter, the application of multi‐functional 3D printing‐based hydrogel dressings in a variety of wound environments is discussed in depth, including hemostasis, anti‐inflammation, antibacterial, skin appendage regeneration, intelligent monitoring, and machine learning‐assisted therapy. Finally, the challenges and prospects of 3D printing‐based hydrogel dressings for wound healing are presented. [ABSTRACT FROM AUTHOR]

Tumor recurrence and wound infection are devastating complications of wide excision surgery for melanoma, and deep postoperative wound drainage typically increases pain. An amoeba‐inspired magnetic soft robot (ASR) with switchable dormant and active phases is developed to address the aforementioned challenges. The dormant ASR supports wounds through its solid‐like elasticity and regulates reactive oxygen species (ROS) levels bidirectionally, promoting healing in infected wounds and eliminating residual tumors. It solves the challenge caused by the contradictory need for ROS scavenging in wound healing and ROS amplification in tumor/infection management. The active ASR removes absorbed wound exudate by crawling out from irregular wounds; interestingly, this crawling motion prevents damage to fragile tissues and alleviates wound pain via "non‐direct friction." More importantly, ASR switches different states in response to an alternating magnetic field owing to its magnetothermal properties, and this process also exerts synergistic antitumor and bacteriostatic effects. Due to the appropriate mechanical structure (cohesive force) of ASR, the content of magnetic nanoparticles required to drive the ASR is ten‐fold lower than that of conventional magnetic soft robots, enabling in vivo degradation. These outcomes highlight the vantage of the ASR for treating post‐tumor excision wounds and underscore their potential for clinical application. [ABSTRACT FROM AUTHOR]

Chitin and chitosan nanofibers (ChNF and CtsNF) are promising biomaterials due to their biocompatibility, biodegradability, and non‐toxicity. This study investigates the cell adhesion properties and inflammatory responses of CtsNF, ChNF, and their mixtures when patterned on cellophane films using inkjet printing technology, keeping in mind their potential applications as cell culture scaffolds. The viscosities of 0.1 wt% aqueous dispersions of CtsNF, ChNF, and their mixtures were confirmed to be suitable for inkjet printing. Microstructures with varying thicknesses were fabricated by adjusting the printing parameters. Mouse fibroblast cells (L929) and mouse macrophages (RAW264.7) were used to evaluate cell adhesion and inflammatory responses. The results demonstrated that CtsNF microstructures exhibited excellent cell adhesion even for those as thin as ~140 nm and low inflammatory potential. This finding provides valuable insights into the development of advanced biomaterials for medical applications and could be instrumental in optimizing dosage settings for wound healing treatments as well. [ABSTRACT FROM AUTHOR]

Background Objectives Methods Results Conclusions Identifier Ablative fractional photothermolysis serves as an excellent in vivo model for studying wound healing. The advent of non‐invasive imaging devices, such as line‐field confocal optical coherence tomography (LC‐OCT), enhances this model by enabling detailed monitoring of skin wound healing over time. Additionally, artificial intelligence (AI)‐based algorithms are revolutionizing the evaluation of clinical images by providing detailed analyses that are unfeasible manually.This study aims to assess the value of combining LC‐OCT and AI for evaluating the acute wound healing process in the skin.The forearms of participating volunteers were ablated with a CO2 laser in a fractional pattern (7.5 mJ/MTZ) (ClinicalTrials.gov identifier: NCT05614557). To induce observable wound healing differences, two different approved silicone‐based formulations were randomly assigned to two test sites, with a third site left untreated. In vivo LC‐OCT images were obtained at predefined intervals post‐laser treatment, ranging from 1 to 7 days. These images were further analysed using AI algorithms.LC‐OCT visualization allows for the characterization of the structural reorganization of the skin during wound healing. The additional integration of AI algorithms significantly enhances the evaluation of the efficacy of wound care interventions by providing a deeper understanding of how these interventions improve wound healing. This is particularly valuable for primary care providers and dermatologists, as AI algorithms have proven useful in observing, characterizing and understanding keratinocyte behaviour.The combination of AI and high‐resolution imaging represents a promising tool for better understanding wound healing, evaluating the efficacy of current wound care interventions and analysing keratinocyte behaviour in detail during the wound healing process.NCT05614557. [ABSTRACT FROM AUTHOR]

Zinc (Zn) is gaining increased recognition as a biodegradable metal in biomedical applications but clinical translation is limited due to its poor biocompatibility. This study addresses these issues through an innovative biomimetic strategy, introducing an efficient surface nanoengineering approach that creates nano‐geometric features and chemical compositions by modulating the exposure time to a biological medium – Dulbecco's Modified Eagle Medium(DMEM). These nanoengineered Zn implants exhibited tunable degradation rates. The nanostructures enhanced human osteoblast attachment, proliferation, and differentiation following direct contact, and improved macrophage function by promoting pseudopod formation and transitioning from a pro‐inflammatory M1 to a pro‐reparative M2 phenotype. In vivo studies show that the surface‐engineered implants effectively promoted tissue integration via M2 macrophage polarization, resulting in a favorable immunomodulatory environment, and increased collagen deposition. Proteomic analyses show that the tissues in the vicinity of the surface‐engineered Zn implants are enriched with proteins related to key wound healing biological mechanisms such as cell adhesion, cytoskeletal structural arrangement, and immune response. This study highlights the improved biocompatibility and anti‐inflammatory effects of surface‐engineered Zn, with important implications for the clinical translation of biodegradable Zn‐based orthopedic implants. [ABSTRACT FROM AUTHOR]

Bacterial infections, especially caused by multidrug‐resistant bacteria, pose a big challenge to the healthcare system. As a group of historic agents, metals with broad‐spectrum antibacterial activity are regarded as promising alternatives to tackle antibiotic resistance. Among them, gallium ions have presented encouraging antibacterial effects in research and preclinic studies. However, utilization of gallium ions has difficulty in achieving high targeting and long‐term effectiveness. With the renaissance of liquid metal, here, a novel and facile antibacterial gallium nanostructure is proposed in which polydopamine‐modified gallium nanocore serves as an ion reservoir for enhanced metal ion release and the surface also permits secondary reaction, allowing for in situ formation of Ag nanoparticles to improve the antibacterial property, ROS generation, and photothermal performance. Notably, ≈100% bacterial killing efficacy can be achieved when combined with NIR laser irradiation. The in vivo treatment results of methicillin‐resistant Staphylococcus aureus (MRSA)‐infected mice demonstrate that the microneedle patch loaded with nanoparticles exhibits outstanding bacterial elimination and inflammation alleviation, and promotes angiogenesis and collagen deposition, further accelerating wound healing. This gallium‐based nanostructure offers an effective nanoplatform for antibacterial treatments and combinatory strategies, which holds significant promise for refractory multidrug‐resistant bacteria and related infections. [ABSTRACT FROM AUTHOR]

Background: Burns are a global public health issue and a major cause of disability and death around the world. Stem cells, which are the undifferentiated cells with the potential for indefinite proliferation and multilineage differentiation, have the ability to replace injured skin and facilitate the wound repair process through paracrine mechanisms. In light of this, the present study aims to conduct a bibliometric analysis in order to identify research hotspots of stem cell–related burns and assess global research tendencies. Methods: To achieve this objective, we retrieved scientific publications on burns associated with stem cells covering the period from January 1, 1978, to October 13, 2022, from the Web of Science Core Collection (WoSCC). Bibliometric analyses, including production and collaboration analyses between countries, institutions, authors, and journals, as well as keyword and topic analyses, were conducted using the bibliometrix R package, CiteSpace, and VOSviewer. Results: A total of 1648 burns associated with stem cell documents were published and listed on WOSCC. The most contributive country, institution, journal, and author were the United States, LV Prasad Eye Institute, Burns, and Scheffer C.G. Tseng, respectively. More importantly, combined with historical direct citation network, trend topic analysis, keyword co‐occurrence network, and substantial literature analysis, we eventually summarized the research hotspots and frontiers on burns associated stem cell reasearch. Conclusion: The present study obtained deep insight into the developing trends and research hotspots on burns associated with stem cells, which arouses growing concerns and implies increasing clinical implications. The mechanism and therapeutics of epidermal stem cells (ESCs) for burn wounds and the mechanism of mesenchymal stem cells (MSCs) and MSC‐derived exosomes for burns wounds are two research hotspots in this field. [ABSTRACT FROM AUTHOR]

An antioxidant, antibacterial, and biocompatible biomaterial is essential to repair skin wounds effectively. Here, we have employed two natural biopolymers, isabgol (ISAB) and konjac glucomannan (KGM), to prepare microporous scaffolds by freezing and lyophilization. The scaffolds are loaded with thymol (THY) to impart potent antioxidant and antibacterial activities. The physicochemical properties of the ISAB+KGM+THY scaffold, like porosity (41.8±2.4 %), swelling, and biodegradation, were optimal for tissue regeneration application. Compared to the control, ISAB+KGM+THY scaffolds promote attachment, migration, and proliferation of L929 fibroblasts. The antioxidant activity of the ISAB+KGM+THY scaffold was significantly improved after loading THY. This would protect the tissues from oxidative damage. The antibacterial activity of the ISAB+KGM+THY scaffold was significantly higher than that of the control, which would help prevent bacterial infection. The vascularization ability of the ISAB+KGM scaffold was not altered by incorporating THY in the ISAB+KGM scaffold. Therefore, a strong antioxidant, antibacterial, and biocompatible nature of the ISAB+KGM+THY scaffold could be useful for various biomedical applications. [ABSTRACT FROM AUTHOR]

Revascularization plays a critical role in the successful engraftment of transplanted pancreatic islets, which are inherently rich in capillaries to meet their high metabolic demands. Innovative islet encapsulation strategies such as the NICHE (neovascularized implantable cell homing and encapsulation), generate a prevascularized transplantation site that allows for direct integration of the graft with the systemic circulation. Timing the transplantation is key to maximizing islet engraftment and survival, especially in diabetic individuals, who exhibit impaired wound healing. Therefore, in this study, we explored different methods to assess vascular development within NICHE in vivo in a non‐invasive fashion. We effectively tracked neoangiogenesis using nanoparticle contrast‐enhanced computed tomography (nCECT), observing a steady increase in vascularization over an 8‐week period, which was confirmed histologically. Next, we estimated relative vascularization changes via T2 mapping with magnetic resonance imaging (MRI) before and after islet transplantation. On the first day post‐transplantation, we measured a slight decrease in T2 values followed by a significant increase by day 14 attributable to islet revascularization. Our findings underscore the potential of non‐invasive imaging techniques to provide insightful information on the readiness of the transplant site within cell encapsulation systems to support cell graft transplantation. [ABSTRACT FROM AUTHOR]

For porous dressing production through 3D printing, it is challenging to adjust the printability of ink while balancing its bioactivity. The present study introduces an example of a modular construction method for heterogeneous granular ink. Gelatin microspheres (gelatin‐MSs) are assembled by poly (gallic acid)‐coated zinc‐containing nano‐hydroxyapatite (ZnHA‐NP/p(GA)), and further cooperate with Ag+ to form a kind of granular inks. The three modules including gelatin‐MS, ZnHA‐NP/p(GA), and Ag have their own functions: The viscoelastic gelatin‐MS module is the essential component that realizes ink extrusion. The ZnHA‐NP/p(GA) module not only forms reversible interactions with gelatin‐MS that realize the assembly but also takes the responsibility of reactive oxygen species‐scavenging and anti‐inflammatory. The Ag module not only interacts with ZnHA‐NP/p(GA) module to promote the stability but also exhibits strong antibacterial property. The gelatin‐MS size, p(GA)‐coating duration, ZnHA‐NP/p(GA) content, and Ag content are optimized to yield a nano‐micro granular ink for bioactive porous dressing preparation that is used to significantly promote the healing of refractory wounds. Therefore, this method allows particles with different structures and bio‐functions that serve as modules to assemble into inks, realizing the balance of printability and biological functions for dressing manufacturing. [ABSTRACT FROM AUTHOR]

Severe skin wounds arising from burns, cancers, and accidents can damage the entire tissue structure, resulting in permanent somatosensory dysfunction in patients. Although emerging hydrogel dressings have shown clinical potential for accelerating wound repair, the use of an individual material to synchronously restore the tissue structure and sensory function of defective skin remains a challenge. Herein, a multifunctional hydrogel that combines electroconductive polydopamine‐capped graphene nanosheets (PrGOs) embedded in a dynamically crosslinked dual‐polysaccharide (xyloglucan and chitosan) matrix network is presented. The fabricated hydrogels have an adjustable modulus that can be matched to skin tissue at the wound site, owing to the dynamic Schiff‐based crosslinking as well as the facile photo‐triggered secondary crosslinking. Furthermore, the photothermal activity of PrGO can elevate the local temperature up to ≈50 °C, significantly restraining bacterial growth. These two factors jointly promote the regeneration of skin tissue. Tissue adhesion of hydrogels is also reported that offers a conformable and robust interface that can detect and quantify human movement and physiological signals to mimic the human skin somatosensory system. This hydrogel offers an effective one‐stone‐for‐two‐birds material that simultaneously achieves tissue regeneration and multi‐signal sensing, promoting the restoration and/or replacement of the structure and function of damaged skins. [ABSTRACT FROM AUTHOR]

The systematic review explores the relationship between smoking and wound healing after surgical treatment of hidradenitis suppurativa (HS), an inflammatory skin disorder. The review analyzed 22 studies, with 16 showing no statistically significant association between smoking and poor surgical outcomes in HS patients. While some studies reported increased adverse outcomes in smokers, there were limitations in matching patients for various factors, indicating insufficient evidence to make concrete recommendations on smoking influences on HS surgical outcomes. Further research with matched patients is suggested to better understand this association. [Extracted from the article]

Jerusalem artichoke (J.A.) tubers contain compounds that exhibit anti‐inflammatory effects and can minimize tissue damage. Ozone is an alternative antimicrobial and immunomodulatory agent for promoting tissue regeneration. The present study aimed to evaluate the therapeutic effect of the ozonated J.A. ointment on a surgically created full‐thickness cutaneous wound in rabbit models. The previously prepared J.A. ointment was ozonated using a Herrmann generator, followed by a subsequent evaluation of its physical and antibacterial properties. Thirty healthy male albino rabbits were used in this study. The animals were divided into two equal groups: the control and treated group. An excisional wound model was used to assess wound healing activities. All of the animals underwent surgical preparation of their dorsal surfaces, and excisional lesions of 3 cm in diameter were created on each animal's dorsal surface of the thoracolumbar region. In the control group, the wounds were left untreated. The animals in the treatment group received a topical application of ozonated J.A. ointment twice daily for five days following the injury. The animals were euthanized on Days 7, 14, and 21 after the injury for histological evaluation. The agar well diffusion method demonstrated the antimicrobial efficacy of the ozonated J.A. ointment. Also, macroscopic and histopathological results showed a significant (p < 0.05) increase in wound area contraction with enhancement re‐epithelization in the treated group compared to the control group. In conclusion, the ozonated ointment derived from J.A. tubers has antibacterial properties and can promote and enhance the wound healing process. [ABSTRACT FROM AUTHOR]

Aim Method Results Conclusion Although various sphincter‐preserving techniques exist for treating anal fistulas, none have demonstrated clear superiority. Therefore, the aim of this study was to introduce a novel sphincter‐preserving technique for anal duct ligation and muscle closure (ALMC) and analyse its perioperative outcomes.The data for patients who underwent ALMC for fistula‐in‐ano at Seoul Song Do Hospital between 2009 and 2023 were retrospectively reviewed. Patient demographics, intraoperative information and postoperative outcomes were assessed. The main outcomes were recurrence and wound healing. Recurrence was defined as the presence of a fistula tract or discharge more than 12 weeks after the primary surgery after achieving complete healing. Faecal incontinence was also investigated clinically.Overall, 556 patients (84.0% male; mean age 41.7 ± 12.3 years) underwent ALMC. Among these, 152 (27.3%) had a history of fistula surgery and 261 (46.9%) had suprasphincteric fistulas. Fistula‐in‐ano recurred in 33 patients (5.9%), wound healing was delayed in 97 (17.4%) and faecal incontinence was observed in 12 (2.2%). The mean follow‐up duration was 10.0 ± 16.0 months, and the average duration until recurrence was 13.8 ± 10.7 months. The proportion of suprasphincteric fistulas was similar in those who experienced recurrence and those who did not (57.6% vs. 46.3%, respectively; p = 0.239). The proportion of suprasphincteric fistulas in the delayed wound healing group was slightly higher, although the differences were not statistically significant (56.7% vs. 44.9%, respectively; p = 0.054).ALMC appeared to be a safe and feasible option for treating anal fistulas, providing good perioperative outcomes, particularly when sphincter preservation was crucial. [ABSTRACT FROM AUTHOR]

Platelet‐rich plasma (PRP) promotes the wound‐healing process and reduces pain. Cord blood platelet‐rich plasma (CB‐PRP), which can be easily collected from the umbilical cord and reapplied to a cesarean section wound, has been proposed to have similar effects as PRP. This paper hypothesizes that CB‐PRB would provide beneficial effects in terms of wound healing and pain reduction in women undergoing cesarean section. This study is a randomized controlled trial involving 52 pregnant women who underwent cesarean sections. Participants were assigned to either the intervention group (n = 26) or the control group (n = 26) at the Obstetrics and Gynecology Clinic of Police General Hospital. Cord blood PRP was applied to the subcutaneous layer and the surgical wound immediately following the cesarean section. The efficacy of wound healing was evaluated using the REEDA scale score on days 1 and 3 postoperatively, and the Vancouver Scar Scale (VSS) was assessed in the 8th week postoperation. The efficacy in reducing pain was measured using a Visual Analog Scale on days 1 and 3 postoperatively. The mean REEDA scale on day 1 (mean ± SD: 1.5 ± 0.2561.5 ± 0.256 in the CB‐PRP group and 2.5 ± 0.267 in the control group; p = 0.009) and the mean VSS score at the 8th week (mean ± SD: 2.577 ± 2.003 in the CB‐PRP group and 6.962 ± 2.441 in the control group; p < 0.001) were significantly lower in the CB‐PRP group than those in the control group. However, there were no differences in Visual Analog Scale values between the two groups. The findings indicate that CB‐PRP potentially promotes wound healing following cesarean sections but does not reduce pain. Further research is needed to confirm the beneficial effects of CB‐PRP. [ABSTRACT FROM AUTHOR]

The repair of diabetic wound still encounters huge challenges, such as disordered inflammatory regulation and impaired neovascularization. Here, a pH/ROS/glucose responsive and photothermal hydrogel is developed for diabetic wound healing. The hydrogel is formed through cross‐linkage between phenylboronic acid‐modified carboxymethyl chitosan (CMCS‐PBA) and oxide dextran (OXD), utilizing Schiff base and phenylboronate ester bonds. Additionally, insulin‐like growth factor 1 C domain (IGF‐1C) and deferoxamine‐loaded polydopamine nanoparticles (D@P) are incorporated into the hydrogel. The hydrogel demonstrates sustained drug release, excellent photo thermal effect, prominent antioxidant, antibacterial and anti‐inflammatory activities, desirable mechanical and tissue adhesive properties, enhanced tube formation, and cell migration. Furthermore, the hydrogel combined with mild heat treatment can regulate chronic inflammation by promoting the transformation of macrophages from M1 phenotype to M2 phenotype and enhance angiogenesis by up‐regulating the expression levels of angiogenesis‐related factors such as hypoxia‐inducible factor‐1 alpha (HIF‐1α), vascular endothelial growth factor (VEGF), CD31, and α‐SMA, thus greatly accelerates the wound healing in streptozotocin (STZ)‐induced diabetic mice. Therefore, this multi‐responsive and multifunctional hydrogel holds potential as a therapeutic strategy for diabetic wounds. [ABSTRACT FROM AUTHOR]

Antibacterial therapy targeting the regulation of macrophage polarization may be a useful approach for normalizing the immune environment and accelerating wound healing. Inspired by black phosphorus‐based nanoplatforms, more stable yet less‐explored violet phosphorus nanosheets (VPNSs) are expected to provide a superior solution for effectively combating bacterial infections. In this study, an average thickness of 5–7 nm VPNSs are fabricated through the liquid‐phase exfoliation method to serve as an immunoregulatory dressing for the treatment of infected wounds. VPNSs attenuated excessive reactive oxygen species (ROS) and reduced the accumulation of proinflammatory M1 macrophages, showing notable antioxidant and anti‐inflammatory properties. Comprehensive RNA sequencing further elucidated the potential immunoregulatory mechanisms of VPNSs, including modulation of the inflammatory response and enzyme regulator activity. Additionally, the inherent photothermal properties of the VPNSs contributed significantly to their antibacterial efficacy. When combined with near‐infrared laser irradiation, VPNSs showed remarkable effectiveness in reducing infection‐related complications and expediting wound healing in infected skin wound models. The rapid promotion of wound healing through ROS clearance, the regulation of macrophage polarization, and hyperthermia generation underscores the potential of the violet‐phosphorus‐based nanoplatforms as clinically viable agents for treating infected wounds. This study suggests that VPNSs are promising candidates for clinical anti‐infective and anti‐inflammatory applications. [ABSTRACT FROM AUTHOR]

Bioprinting technology plays a crucial role for constructing tissue substitutes. However, the mismatched scaffold shapes and the poor treatment timeliness limit its clinical translational application. In situ printing technology that prints bioregenerants directly inside patient's body can meet the needs of specific tissue repair. This study develops a smartphone controlled handheld bioprinter for in situ skin wounds dressing. The mini bioprinter can be handheld and placed on any printing surface to create strips, complex patterns, and 3D structures, and can be equipped with microchannel needles to expand functionality. The size of the strips as well as the printing path can be programmed and controlled by the smartphone to ensure the precision of the printed product quality. Furthermore, the device not only allows for smooth switching between different bioinks for printing heterogeneous structure, but also allows for fast and uniform coverage of large wound surfaces. When dealing with complex wounds in vitro & vivo, the printer can effectively fill and precisely close wounds, promoting wound healing. The programmable handheld bioprinter can balance mobility and customizability in the management of skin wounds and is expected to realize its potential for emergency medical treatment in condition‐constrained scenarios, such as battlefields or disaster areas. [ABSTRACT FROM AUTHOR]

ABSTRACT Background Materials and Methods Results Conclusion The original TriHex combination—Tripeptide‐1 and Hexapeptide‐12 (TriHex) encompasses a peptide combination selected for its ability to modulate the extracellular matrix (ECM) by progressively eliminating clumped collagen and elastin fragments and then stimulating replacement with new collagen and elastin. Incorporation of a proprietary, patent‐pending Octapeptide‐45 (Octa) to the TriHex original provides potential for added benefit based on the peptide's capacity to stimulate hyaluronic acid (HA) and its anticipated added benefit in wound healing. This is named TriHex 2.0 in the paper.A full‐scale validation process was structured to assess Octa synergy with TriHex using an ex vivo model, assessing ECM changes histologically in relation to elastin, HA and basement membrane components. In addition, gene expression studies were undertaken, including bulk and single cell sequencing analysis to assess the particular changes that occurred by adding Octa to the TriHex. Following the gene expression analysis, a further round of ex vivo studies was conducted to assess protein expression of the defined differentially expressed genes using histological staining.Octa synergized with TriHex as demonstrated by significantly upregulated genes (p < 0.05) affecting the ECM and basement membrane. A histological assessment using the ex vivo model demonstrated tropoelastin intensity significantly increasing with TriHex (43%) and 2.0 (42%) (p < 0.05 for both) compared to untreated explants. HA levels (CD44 intensity) significantly increased with TriHex (69%; p < 0.01), while TriHex 2.0 demonstrated HA levels 160% greater (p < 0.001) than the untreated tissue. Single cell sequencing identified a gene expression profile upregulation relating to ECM modulation and wound healing in both TriHex and 2.0, but TriHex 2.0 showed additional activities in basement membrane physiology, stem cell recruitment, and protection of fibroblasts against cellular senescence.The addition of Octapeptide‐45 to TriHex technology in the form of TriHex 2.0 is a significant advance to TriHex technology science. Both forms demonstrate ECM remodeling and positive wound healing, but supplementary benefits are evident including increased elastin and hyaluronic acid stimulation, added effects on the basement membrane, additional wound healing capacity in basal keratinocytes and anti‐senescent effects in fibroblasts. This is helpful for pre‐conditioning of the skin prior to procedures and post procedure related to additional ECM remodeling, wound healing advantages, senescent cell targeting and DEJ strengthening. Clinical studies to follow. [ABSTRACT FROM AUTHOR]

ABSTRACT Aim Materials and Methods Results Conclusion The aim of this study was to investigate the non‐inferiority of alveolar ridge preservation (ARP) with membrane stabilization compared with non‐stabilization, focusing on changes of soft‐tissue contour in periodontally compromised extraction sockets. Secondary outcomes included changes in hard‐tissue contour, patient‐reported outcomes and new bone formation.Twenty‐four patients with periodontally compromised teeth were randomly assigned to ARP with (test group) or without (control group) membrane stabilization. To assess profilometric and hard‐tissue dimensional changes, dental impressions and cone beam computed tomography scans were performed at baseline (T0), immediately after ARP (T1) and 4 months post surgery (T2). Soft‐tissue healing in open healing sites was evaluated at T2, and wound closure was assessed 10 days post surgery. Patient‐reported outcomes were documented, and core biopsies were obtained for histomorphometric analysis.The absolute profilometric horizontal width change at 3 mm below the crest in the test group was not inferior to that in the control group. For the relative values, horizontal width reduction (3 and 5 mm below the crest) and volumetric shrinkage (3–5 mm below the crest) were lower in the test group. No significant differences were observed in bone dimensional changes, wound healing, pain and swelling or histomorphometric outcomes.ARP with membrane stabilization in periodontally compromised extraction sockets is non‐inferior in terms of soft‐tissue contour changes to those without membrane fixation.Trial Registration: Clinical Research Information Service (CRIS), KCT0005280. Registered 4 August 2020, https://cris.nih.go.kr/cris/search/detailSearch.do?seq=19165&search_page=L [ABSTRACT FROM AUTHOR]

We synthesized a novel compound library featuring a spirooxindole core structure combined with various heterocycles, including benzofuran, benzothiophene, and thiophene scaffolds. Evaluation using MTT assays against HepG2, 4T1, and MDA‐MB‐231 cells revealed the most potent candidate, spirooxindole hybrid 5c, with an IC50 of 5 ± 0.6 µM against HepG2, inducing G2/M phase cell cycle arrest, inhibition of the wound healing, and induction of ROS. Selected spirooxindole conjugates exhibited significant inhibitory potential against MDM2, with KD values ranging from 0.0531 to 16.8 µM. Notably, the salt of spirooxindole analogue 5q demonstrated the highest inhibitory activity at KD = 53.1 nM. Molecular docking studies revealed excellent accommodation of the designed compounds within the MDM2 receptor. All compounds displayed favorable ADME profiles, suggesting their potential as lead compounds for further optimization. [ABSTRACT FROM AUTHOR]

Near‐infrared laser‐activated gold nanorods (AuNRs) with excellent photothermal property and tunable surface functionalization are considered as an ideal platform for biomedical applications. However, bare AuNRs have cytotoxicity against normal cells and are prone to agglomeration during laser irradiation. Herein, multivalent polymer‐functionalized AuNRs (AuNRs@pDMAEMA‐C4) was constructed as a highly stable and biocompatible photothermal agent for enhanced antibacterial therapy. The functionalized polymer was synthetized via the reversible addition‐fragmentation chain transfer polymerization and subsequently quaternized. Moreover, positively charged AuNRs@pDMAEMA‐C4 can easily capture the bacterial surface via electrostatic interactions. The integration of photothermal therapy of AuNRs and chemotherapy of functionalized polymer can achieve enhanced antibacterial effects. Under 808 nm laser irradiation, AuNRs@pDMAEMA‐C4 possessed excellent photothermal conversion capability and can kill gram‐positive and gram‐negative bacteria. Study of the antibacterial mechanism indicated that the antibacterial action of the prepared photothermal antibacterial agent can cause serious damage of the bacterial outer membranes, result in cytoplasm leakage and bacterial death. The nanocomposites combining with near‐infrared laser irradiation can facilitate rapid healing of bacteria‐infected wound by rat model of wound infection and histological analysis of the wound tissues. These results suggest that the surface functionalization can be used as potential strategy to fabricate light‐activated therapeutic agent for biomedical applications. [ABSTRACT FROM AUTHOR]

Photothermal therapy (PTT) is widely used in wound healing because it produces heat under near‐infrared irradiation, eliminating bacterial infections. However, excessive production of reactive oxygen species (ROS) occurs during PTT, which causes oxidative stress damage. In this study, a nanozyme, PDA‐MnO2 (PM), is synthesized and encapsulated in the needle tip layer of a bi‐layer GelMA microneedle patch (PbMP), which has excellent mechanical properties and can easily penetrate the skin. In a study involving an SD rat wound model infected with S. aureus, the PbMP significantly accelerated the healing process of bacterially challenged wounds, resulting in better blood vessel growth, cell proliferation, and collagen biosynthesis than other wound dressings. The experiment shows that the PM nanoparticles in PbMP improve photothermal conversion efficiency and activate SOD and CAT, resulting in an efficient cascade enzyme reaction system to eliminate ROS during the PTT process. This study describes a highly effective nanozyme hydrogel microneedle platform with photothermal synergistic antibacterial effects on wound healing. [ABSTRACT FROM AUTHOR]

Fungal infections pose a formidable challenge in the healing of diabetic wounds due to complex inflammatory microenvironments, high resistance, and recurrence risks, particularly during fungal aggregation and germination stages. Inspired by controllable fishhooks, this work presents a magnetically actuated microrobot (Co@NCNH/NC) that leverages carbon nanohooks to enhance localized photothermal therapy. This microrobot features controllable navigation capabilities to pathogens in the magnetic field, enabling precise fungal capture and thermally promoted penetration even in high‐viscosity environments. With impact force and high conductivity, the microrobot's nanohooks induce disruption of cell envelopes and facilitate electron extraction from membranes, collectively compromising fungal integrity and enhancing fungal vulnerability. Thus, in situ, heat ablation further eradicates aggregated spores and causes irreversible damage to germinated fungi by aggravating cellular lysis, metabolic dysfunction, and destruction of actin cytoskeletons, inhibiting fungal self‐repair and growth. Moreover, Co@NCNH/NC with good biocompatibility exhibits catalase‐like activity that helps alleviate wound inflammation during therapy. This dual‐action approach accelerates critical wound healing processes including angiogenesis and epidermalization, ultimately improving healing outcomes in chronic diabetic wounds. By combining targeted fungal eradication with inflammation reduction and tissue regeneration, this adaptable microrobotic technology shows considerable promise for advancing therapeutic efficacy and prevention of recurrence in diabetic wound healing. [ABSTRACT FROM AUTHOR]

ABSTRACT Background Methods Results Conclusion Polynucleotides (PN), popular in biorevitalization, show promise in the current sphere of esthetic medicine due to their regenerative properties, previously used in wound healing. Currently, research investigates their use in esthetic medicine. The aim of this review was to synthesize the existing literature, focusing on the effectiveness of PN in esthetic medicine, which is concentrated on skin rejuvenation by providing patients with multiple benefits and the least side effects.A systematic electronic search was conducted in Embase, Medline, and Cochrane to identify primary research studies evaluating the effectiveness of polynucleotides in esthetic medicine, published in English between January 01, 2010, and January 01, 2024. A narrative synthesis was reported according to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses statement. The quality of evidence was assessed using the Critical Appraisal Skills Programme checklists. PROSPERO Registration: CRD42024588712.Nine studies, of low and moderate quality, were included in this review, describing a population of 219 patients receiving PN treatment. A variation was present regarding procedural characteristics, such as injection areas and techniques. Polynucleotide injections have shown promising outcomes in reducing wrinkles, improving skin texture, and enhancing elasticity, with statistically significant results in several studies. While side effects are generally mild and transient, patient satisfaction is moderate to high, suggesting PN treatment as a well‐tolerated and effective cosmetic intervention.Polynucleotides offer promising potential in esthetic medicine; however, there is limited consensus regarding their optimal use. Rigorous, high‐quality studies are essential to validate the effectiveness and safety of PN. [ABSTRACT FROM AUTHOR]