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Passive radiative cooling is a zero‐energy consumption approach, which can dissipate heat to outer space by emitting infrared radiation through the transparency window. Traditional cooling materials, such as photonic films, metafabrics, and polymer foams, still suffer from complex preparation processes and high costs. In this work, it is reported that natural cellulose can be converted into a “green” optical metamaterial by rational structure reconfiguration at the micro/nano level via scalable ball milling technology for efficient daytime radiative cooling. Specifically, fine‐tuning the shearing kinetics in the mechanochemistry process, cellulosic optical metamaterial (COM) with ≈98% solar reflectivity and ≈0.97 infrared emissivity has been successfully achieved, which can break through the theoretical value of photonic crystals as well as the conventional synthetic optical materials. The COMSOL simulation reveals that the excellent optical properties of the cellulose metamaterial are explained by the “confined scattering” effect caused by the rearranged heterostructure at the micro/nano level. Outdoor tests demonstrat that the COM‐based coating exhibits a daytime radiative cooling efficiency of 5.7 °C in hot Nanjing. Meanwhile, the COM can be produced into different scattering materials via spray coating, freeze casting, and solution casting technology. This study will facilitate the development of scalable and sustainable optical metamaterials for mitigating energy consumption. [ABSTRACT FROM AUTHOR]

Cancer recurrence and metastasis after surgical resection is a vital reason of treatment failure. The modification of immune cells through implanted biomaterials is a promising postoperative immunotherapy. Herein, an injectable hydrogel scaffold loaded with engineered exosome mimetics that in vivo recruits and programs endogenous macrophages into M1 binding with anti‐CD47 antibody (M1‐aCD47 macrophages) for postoperative cancer immunotherapy is developed. Briefly, M1 macrophages‐derived exosome mimetics co‐modified with vesicular stomatitis virus glycoprotein (VSV‐G) and aCD47 (V‐M1EM‐aCD47) are encapsulated in injectable chitosan hydrogel. Such hydrogel recruits inherent macrophages in situ and releases V‐M1EM‐aCD47 that programs M2 to M1‐aCD47 macrophages. M1‐aCD47 macrophages own dual‐functions of tumor‐homing and enhanced phagocytosis. They can actively target to tumor cells for delivery of aCD47 that blocks the "don't eat me" signal, thereby promoting phagocytosis of macrophages to cancer cells. Furthermore, V‐M1EM‐aCD47 hydrogel implanted into resection site of 4T1 breast tumor inhibits tumor recurrence and metastasis by phagocytosis of M1‐aCD47 macrophages and T cell‐mediated immune responses. The findings demonstrate that biomaterials can be designed in vivo to program inherent macrophages, thereby activating the innate and adaptive immune systems for prevention of postoperative tumor recurrence and metastasis. [ABSTRACT FROM AUTHOR]

Summary: The very high concentrations required for industrial production of free acetic acid create toxicity and low pH values, which usually conflict with the host cell growth, leading to a poor productivity. Achieving a balance between cell fitness and product synthesis is the key challenge to improving acetic acid production efficiency in metabolic engineering. Here, we show that the synergistic regulation of alcohol/aldehyde dehydrogenase expression and cofactor PQQ level could not only efficiently relieve conflict between increased acetic acid production and compromised cell fitness, but also greatly enhance acetic acid tolerance of Acetobacter pasteurianus to a high initial concentration (3% v/v) of acetic acid. Combinatorial expression of adhA and pqqABCDE greatly shortens the duration of starting‐up process from 116 to 99 h, leading to a yield of 69 g l‐1 acetic acid in semi‐continuous fermentation. As a final result, average acetic acid productivity has been raised to 0.99 g l‐1 h‐1, which was 32% higher than the parental A. pasteurianus. This study is of great significance for decreasing cost of semi‐continuous fermentation for producing high‐strength acetic acid industrially. We envisioned that this strategy will be useful for production of many other desired organic acids, especially those involving cofactor reactions. [ABSTRACT FROM AUTHOR]

Objective: The study aimed to examine the relationship between secondhand smoke (SHS) exposure and severe headaches or migraine in never‐smoking adults verified by serum cotinine. Background: Current evidence about the association between self‐reported SHS exposure and headaches or migraine is limited and contradictory. An important issue lies in the lack of actual SHS exposure assessment through biomarkers. Methods: We conducted a cross‐sectional study on 4560 never‐smoking adults from the National Health and Nutrition Examination Survey (NHANES), 1999–2004. The SHS exposure was evaluated by measuring serum cotinine concentrations. The information regarding severe headaches or migraine was based on self‐reporting. Results: The overall prevalence rate of severe headaches or migraine was 20% (919/4560). After adjusting for relevant covariates, we found that heavy SHS exposure (serum cotinine at 1 to 10 ng/mL) was positively associated with severe headaches or migraine (OR: 2.02, 95% CI [1.19, 3.43]); however, no significant association was found between low SHS exposure (serum cotinine at 0.05 to 0.99 ng/mL) and severe headaches or migraine (OR: 1.15, 95% CI [0.91, 1.47]). Restricted cubic spline analysis showed that the natural logarithm of serum cotinine had a linear relationship with severe headaches or migraine (p = 0.335 for nonlinearity). Stratified analysis indicated that individuals with a BMI of <25 (p < 0.001 for interaction) and sedentary activity (p = 0.016 for interaction) modified the relationship between SHS exposure and severe headaches and migraine. Even after altering the definition of SHS exposure, excluding drugs that might affect the metabolism of serum cotinine, and multiple imputation, our sensitivity analysis results remained stable. Conclusions: The study demonstrated that heavy SHS exposure (serum cotinine at 1 to 10 ng/mL) had a significant positive association with severe headaches or migraine in never‐smoking adults. Prospective studies are necessary to verify this relationship in the future. [ABSTRACT FROM AUTHOR]

Vegetation growth is influenced by the microclimate driven by aspects, as evident in the asymmetric vegetation greenness on polar‐facing slopes (PFS) and equatorial‐facing slopes (EFS). However, it remains uncertain whether aspects influence vegetation phenology. To address this question, we defined the aspect‐induced phenological differences between PFS and EFS from 2019 to 2022 within each 3 × 3 km2 grid, using average phenological metrics extracted from Sentinel‐2 data. We found that the start of the growing season (SOS) occurs earlier on EFS in cold and humid regions, but in arid areas, PFS has an earlier SOS. The end of the growing season (EOS) consistently occurred later on EFS due to radiation limitations in autumn phenology. Employing the space‐for‐time approach, the observed distribution of phenological differences within the climate space could potentially indicate the phenological trends of different slope orientations in the future. Our study provides valuable insights into topographic regulation on vegetation phenology. Plain Language Summary: Equatorial‐facing slopes (EFS) receive more solar radiation than polar‐facing slopes (PFS), resulting in contrasting microclimate conditions. Specifically, EFS are warmer and drier, while PFS are colder and wetter. These microclimate differences contribute to variations in vegetation greenness between PFS and EFS. Based on this, we hypothesized that the phenology of grassland in the Three Rivers Source Region on the Tibetan Plateau is influenced by aspects. To test this hypothesis, we calculated the phenological differences between PFS and EFS within each 3 × 3 km2 grid using average phenological metrics derived from Sentinel‐2. Our findings reveal that, for the start of the growing season (SOS), EFS exhibited an earlier onset in regions with low temperature and high precipitation, whereas for regions with high temperature, the growing season starts earlier on PFS. In contrast, over 70% of the grassland area on EFS experiences a later end of the growing season (EOS) due to radiation being a major limiting factor of autumn phenology. In addition, we utilized the space‐for‐time approach to project potential future phenological changes on PFS and EFS. Our study enhances comprehension of vegetation ecological management and carbon sequestration in mountainous areas. Key Points: There are significant phenological differences between polar‐facing slopes and equatorial‐facing slopesPhenological differences vary under different background climatic conditionsUsing the space‐for‐time approach, the phenological differences in climate space suggest future phenological shifts [ABSTRACT FROM AUTHOR]

Objective: Despite the benefits, the rate of genetic testing among first‐degree relatives (FDRs; parents, children, and siblings) remains low, and the barriers to undergoing testing among FDRs in China are not clear. We explored the reasons why FDRs refused genetic testing. Methods: Semi‐structured face‐to‐face interviews were conducted with 22 patients and 27 FDRs. Participants were recruited at an urban tertiary hospital in Guangzhou, South China. We used qualitative content analysis to analyse the transcripts of audio recordings and identify major themes and subthemes. Results: Three major themes emerged related to FDRs' low rate of participation in genetic testing. First, there is cognitive distance from genetic testing/cancer and a lack of knowledge of preventive medicine that deepens the 'fatalistic' attitude towards cancer among FDRs, which leads to an enormous gap between their knowledge and understanding of genetic testing. Second, medical consultation is not valued in Confucianism, and the view of cancer as 'bad news' and the risk of cancer as a curse makes cancer a metaphor, which leads to exhausting arguments when persuading FDRs to undergo genetic testing. Third, physical distance from the hospital, loss of privacy, possible discrimination in many social activities and genetic testing as a source of stress and anxiety lead FDRs to fear the disruption of their daily lives. Conclusions: There are many barriers to genetic testing among the FDRs of hereditary cancer patients originating from the national social and cultural context. Healthcare professionals should develop interventions rooted in culture and promote cancer risk communication between hereditary cancer patients and FDRs. [ABSTRACT FROM AUTHOR]

BACKGROUND The application of Pickering emulsions stabilized by food‐derived particles is of great interest, studies have focused on development of natural functional emulsifiers from agricultural byproducts. Dietary fiber (DF) has been recognizing for its excellent physiological functions. Moreover, physicochemical properties of pomelo peel DF (PDF) make it a potential emulsifier. However, pristine PDF is not suitable as emulsifier due to its compact physical structure and high hydrophobicity, which seriously limits its utilization. The objective of the study was to investigate the effects of cellulase on physicochemical properties of PDF and to illustrate the feasibility of cellulase modified PDF (MPDF) as natural functional emulsifier. RESULTS: Cellulase modification significantly improved (P < 0.05) specific surface area, water‐holding capacity/oil‐holding capacity, viscoelasticity, hydrophobicity, and pore structure while decreased crystallinity index and particle size of PDF. Emulsion could remain stable over 30 days as MPDF concentrations up to 1 wt% and oil/water ratio 3:7. The appearance stability of emulsions was not influenced by temperature (4–60 °C), pH (3.0–12.0), and ion concentration (0–200 mmol L−1) which was similar to Pickering emulsions. The mechanism of MPDF as an emulsifier was mainly attributed to the combination of Pickering effect and the three‐dimensional network. In addition, MPDF showed higher antioxidant capacity in emulsions than other classical emulsifiers. CONCLUSION: The results illustrated that MPDF has a favorable feasibility for preparation of stable Pickering‐type emulsions, which will be a practical support for application of PDF as a natural functional emulsifier and will be helpful to realize the resource utilization of DF in pomelo industries. © 2022 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

The development of a simple and rapid separation and analysis method with high selectivity toward ciprofloxacin is of great importance for environmental protection. Herein, this paper reported a hollow magnetic zeolitic imidazolate framework‐67 based molecularly imprinted polymer for selective adsorption and enrichment of ciprofloxacin. The morphology and structural properties of the hollow magnetic molecularly imprinted polymer were characterized by scanning electron microscopy, transmission electron microscopy, infrared spectroscopy, and thermogravimetry, and the results showed that the material has an obvious hollow structure, good thermal stability, and magnetic properties. The hollow magnetic imprinted polymer has a higher adsorption capacity (75.59 mg/g) and shorter adsorption saturation time (50 min) toward ciprofloxacin. Moreover, the hollow magnetic molecularly imprinted polymer showed excellent adsorption ability, stability, and repeatability. The hollow magnetic imprinted polymer was used to detect trace ciprofloxacin in actual samples (river water, human urine, chicken) successfully with the recoveries of 91.4–100.4%. With the advantages of high recovery (91.4–100.4%), high speed (50 min), and high selectivity (selectivity factor > 2.50), the hollow magnetic molecularly imprinted polymer has broad application prospects in the efficient adsorption of ciprofloxacin. [ABSTRACT FROM AUTHOR]

BACKGROUND Tannase is an enzyme produced by microbial fermentation and is widely used in the food industry; however, the molecular mechanism of tannase production by Aspergillus has not yet been studied. This study was conducted to reveal the differences in Aspergillus carbonarius tannase enzymatic characterization, secondary structures and molecular mechanisms after treatment of the strain with atmospheric and room temperature plasma (ARTP). RESULTS: The results showed that the specific activity of tannase was improved by ARTP treatment, and it showed higher thermostability and tolerance to metal ions and additives. The enzymatic characterization and molecular docking results indicated that tannase had a higher affinity and catalytic rate with tannic acid as a substrate after ARTP treatment. In addition, the docking results indicated that Aspergillus tannases may catalyze tannic acid by forming two hydrogen‐bonding networks with neighboring residues. RNA‐seq analysis indicated that changes in steroid biosynthesis, glutathione metabolism, glycerolipid metabolism, oxidative phosphorylation pathway and mitogen‐activated protein kinase signaling pathways might be crucial reasons for the high production of tannase. CONCLUSION: ARTP enhanced the yield and properties of A. carbonarius tannase by changing the enzyme structure and cell metabolism. This study provides a theoretical basis for elucidating the molecular mechanism underlying high production of Aspergillus tannases. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

A new phenolic digycoside 1 was produced as stress metabolite in the fresh leaves of Viburnum ichangense ( Hemsl.) Rehd., in response to abiotic stress elicitation by CuCl2. The stress metabolite was characterized as 1- O-[ α- L-arabinofuranosyl(1→6)- β- D-glucopyranosyl]- erythro-1,2-bis(4-hydroxy-3-methoxyphenyl)propane-1,3-diol ( 1). A new flavan dimer, 2,3-epoxyflavan-3′,4′,5,7-tetraol-(4→8″)-flavan-3″,3′′′,4′′′,5′′′,6″-pentaol ( 2), and two known compounds, hovetrichoside A ( 3) and asperglaucide ( 4), were also isolated. Their structures were established by spectroscopic means. [ABSTRACT FROM AUTHOR]

Summary: Elevated atmospheric carbon dioxide (eCO2) concentrations promote symbiosis between roots and arbuscular mycorrhizal fungi (AMF), modifying plant nutrient acquisition and cycling of carbon, nitrogen and phosphate. However, the biological mechanisms by which plants transmit aerial eCO2 cues to roots, to alter the symbiotic associations remain unknown.We used a range of interdisciplinary approaches, including gene silencing, grafting, transmission electron microscopy, liquid chromatography tandem mass spectrometry (LC–MS/MS), biochemical methodologies and gene transcript analysis to explore the complexities of environmental signal transmission from the point of perception in the leaves at the apex to the roots.Here we show that eCO2 triggers apoplastic hydrogen peroxide (H2O2)‐dependent auxin production in tomato shoots followed by systemic signaling that results in strigolactone biosynthesis in the roots. This redox‐auxin‐strigolactone systemic signaling cascade facilitates eCO2‐induced AMF symbiosis and phosphate utilization.Our results challenge the current paradigm of eCO2 effects on AMF and provide new insights into potential targets for manipulation of AMF symbiosis for high nutrient utilization under future climate change scenarios. See also the Commentary on this article by Hause, 224: 5–7. [ABSTRACT FROM AUTHOR]

Pulmonary hypertension (PH) is prevalent in patients with obstructive sleep apnea (OSA) syndrome, and coexistence of PH and OSA indicates a worse prognosis and higher mortality. Chronic intermittent hypoxia (CIH) is the key pathogenesis of OSA. Also, microRNA‐223 (miR‐223) plays a role in the regulation of CIH‐induced PH process. However, the detailed mechanism of CIH inducing PH is still unclear. This study aimed to investigate the pathological process of CIH associated PH and explore the potential therapeutic methods. In this study, adult Sprague–Dawley rats were exposed to CIH or normoxic (N) conditions with 2‐methoxyestradiol (2‐Me) or vehicle treatment for 6 weeks. The results showed that 2‐Me treatment reduced the progression of pulmonary angiogenesis in CIH rats, and alleviated proliferation, cellular migration, and reactive oxygen species formation was induced by CIH in pulmonary artery smooth muscle cells (PASMCs). CIH decreased the expression of miR‐223, whereas 2‐Me reversed the downregulation of miR‐223 both in vivo and in vitro. Furthermore, the antiangiogenic effect of 2‐Me observed in PASMCs was abrogated by miR‐223 inhibitor, while enhanced by miR‐223 mimic. These findings suggested that miR‐223 played an important role in the process of CIH inducing PH, and 2‐Me might reverse CIH‐induced PH via upregulating miR‐223. The role of 2‐methoxyestradiol (2‐ME) was investigated in a model of pulmonary hypertension (PH) induced by chronic intermittent hypoxia. We found that 2‐ME markedly reduced PH progression, and this effect is associated with reduced microRNA‐223 (miR‐223). [ABSTRACT FROM AUTHOR]

Chemically mediated interactions have been hypothesized to be essential for ecosystem functioning as co-occurring organisms can influence the performance of each other by metabolic means. Here, we present a co-culture device that allows co-culturing of microorganisms that are physically separated but can exchange chemical signals and metabolites. This setup was adopted to perform investigations on the secondary metabolisms of both a fungal-bacterial community and an actinomycetic-actinomycetic community. This study employed a metabolomics approach integrating LC-MS profiling, multivariate data analysis and molecular networking techniques. LC-MS measurements revealed a pronounced influence of such chemical communication on the metabolic profiles of synthetic co-culture communities with a group of molecules being induced or upregulated in co-cultures. A novel antibiotic exhibiting antibiotic properties against Klebsiella pneumoniae was unveiled in the fungal-bacterial community. Besides, a further survey of the fungal-bacterial cross-talk indicated that the production of co-culture-induced diphenyl ethers by fungi might result from the fungal response against the secretion of surfactins by bacteria in the cross-talk. This study demonstrated that the presented co-culture device and the metabolomic routine would facilitate the investigation on chemically mediated interactions in nature as well as cryptic natural products discovery. [ABSTRACT FROM AUTHOR]

The magnetic and macro/mesoporous bioactive glasses scaffolds are synthesised successfully by the combination of coral and P123 as co‐templates through an evaporation‐induced self‐assembly process. The prepared material can induce the precipitation of hydroxyapatite layers on their surface in SBF only within 12 h. At the same time, the material exhibited excellent super‐paramagnetic and mechanical property. Furthermore, the biocompatible assessment confirmed that the obtained material presented the good biocompatibility and the enhanced adherence of HeLa cells. Herein, the novel materials are expected to have potential application for bone tissue engineering. [ABSTRACT FROM AUTHOR]

Reverse transformation at 725-800°C × 0-35 s from a ferrite/pearlite to austenite was investigated in spring steel 60Si2MnA with various pearlite fineness obtained by isothermal transformation. Then, its influence on the structure inheritance in the spring steel during heat treatment process was discussed. The results indicated that various pearlite fineness influences clearly start temperature of the reverse transformation. Austenite preferentially nucleates at the ferrite/cementite interface within pearlite, the neighboring pearlite colony boundary and the proeutectoid ferrite/pearlite boundary during the reverse transformation process. And the angle between pearlite lamellae and austenite growth direction has an important influence on austenite growth rate. Besides, the ferrite/pearlitic structure has structure inheritance phenomenon, because various hot-rolled microstructure affect the austenite nucleation and growth during heat treatment process. [ABSTRACT FROM AUTHOR]