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The genomic and haplotype sequencing results showed that the patient was heterozygous in the I ABO i gene for a newly identified I B i allele (GenBank accession number MN971575) with the common I ABO*O.01.02 i allele on the other chromosome. ABO is the most important blood group system with clinical relevance in transfusion, prenatal care, bone marrow, and organ transplantation.1 Most ABO subtypes are caused by single-nucleotide substitutions in exons 6 and 7 of the I ABO i gene,2 which can reduce the activity of ABO glycosyltransferase. [Extracted from the article]

Nucleotide sequencing of I RHCE i Exon 1 showed a C nucleotide at position c.48 associated with an I RHCE*C i allele and a standard I RHCE*C i sequence in Exon 2. The I RHCE i gene coding sequences and their adjacent intron regions were amplified by PCR and the nucleotide sequences obtained as published previously.4 We determined I RHD i zygosity by PCR-restriction fragment length polymorphism (RFLP) for the presence of the hybrid Rhesus box. A null allele caused by a four-base-pair duplication within the RHCE gene encoding a D- - phenotype. [Extracted from the article]

Background: Human neutrophil antigen-3 (HNA-3) alloantibodies can cause fatal transfusion-related acute lung injury (TRALI). Most frequencies of SLC44A2 alleles encoding the HNA-3a/b antigens have been established in Han individuals by polymerase chain reaction with sequence-specific priming (PCR-SSP). We sequenced SLC44A2 gene fragments and determined allele frequencies in three ethnicities of China.Study Design and Methods: Genomic DNA was extracted from 448 samples of 100 blood donors of Yi ethnicity in Xichang, Liangshan; 248 Han in Nanjing, Jiangsu; and 100 Tibetan in Lhasa, Tibet. A PCR-SSP was applied to determine the phase of two single-nucleotide polymorphisms (SNPs); SLC44A2 haplotypes were constructed.Results: In the 567 nucleotides of the SLC44A2 gene covered by our sequencing approach in Han individuals, we detected the known 331-44G>A (rs12972963) and 461G>A (rs2288904) polymorphisms. In the 243 nucleotides sequenced in Yi and Tibetan populations, we detected the known 461G>A and 503-15T>C (rs1560711) polymorphisms. A PCR-SSP for the common HNA-3a/b SNP was 100% concordant. The frequencies of the HNA-3a allele were 0.58, 0.66, and 0.69 in Yi, Han (Nanjing), and Tibetan, respectively (0.42, 0.34, and 0.31 for HNA-3b).Conclusions: The Yi population of China had the highest frequency of blood donors at risk of harboring anti-HNA-3a compared to any population studied so far. We confirmed that the underlying SLC44A2*2 allele is more common in China than in any European or African populations. [ABSTRACT FROM AUTHOR]

Background Antiangiogenesis plays a key role in the treatment of non-small lung cancer ( NSCLC). We observed the cavitation of lesions in patients with stage IIIB/ IV NSCLC treated with Endostar and vinorelbine-cisplatin ( NP) chemotherapy, and evaluated the imaging characteristics and clinical outcome of patients who developed tumor cavitation. Methods Our study included 105 untreated NSCLC patients who received Endostar in combination with NP chemotherapy at the Tianjin Lung Cancer Center. Chest computed tomography ( CT) was performed to evaluate the efficacy every two cycles. The number of activated circulating endothelial cells ( aCECs) was measured by flow cytometry. Rates of tumor cavitation were documented and their clinical CT imaging data were analyzed. Results Tumor cavitation occurred in 11 of the 105 (10.5%) patients treated with Endostar and NP. The response rates were 37.2% (35/94) in patients without cavitation, 27.3% (3/11) evaluated by Response Evaluation Criteria in Solid Tumors, and 100.0% (11/11) if evaluated by an alternate method in patients who developed cavitation. Three of the 11 cases with cavitation had a centrally located tumor. No patients had hemoptysis or any other severe side effects. Compared with patients not developing cavitation, cavity formation resulted in a longer median survival time (13.6 vs. 11.8 months, P = 0.011) and an increase in the number of aCECs (244.4/105 vs. 23.3/105, P = 0.000). Conclusions Intratumoral cavitation induced by Endostar is common in NSCLC patients, and is not correlated with squamous histology, tumor location or pulmonary hemorrhage. Cavitation might have a significant effect on the number of aCECs and overall prognosis. [ABSTRACT FROM AUTHOR]

The P1PK blood group system (ISBT 003) comprises P1, P SP k sp , and NOR antigens, all synthesized by the glycosyltransferase Gb3/CD77 enzyme. The c.547 548delAT allele has previously been found which also contained an additional alteration c.367T>C giving rise to p.Ser123Pro but does not change the phenotype in two samples from Maghreb.4 This allele was named I A4GALT*02N.07 i by ISBT. Our study demonstrated that our patient with the rare p phenotype was compound heterozygous for two nonfunctional I A4GALT i alleles. [Extracted from the article]

The current criteria for evaluating antiangiogenic efficacy is insufficient as tumor shrinkage occurs after blood perfusion decreases. Tumor blood volume ( BV) in computed tomography perfusion imaging and circulating endothelial cells ( CEC) might predict the status of angiogenesis. The present study aimed to validate their representation as feasible predictors in non-small-cell lung carcinoma ( NSCLC). A total of 74 patients was categorized randomly into two arms undergoing regimens of vinorelbine and cisplatin (Navelbine and platinum [ NP]) with rh-endostatin or single NP. The response rate, perfusion imaging indexes and activated CEC (a CEC) during treatment were recorded. Progression-free survival ( PFS) was determined through follow up. Correlations among the above indicators, response and PFS were analyzed: a CEC increased significantly in cases of progressive disease after single NP chemotherapy ( P = 0.024). Tumor BV decreased significantly in cases with a clinical benefit in the combined arm ( P = 0.026), whereas inverse correlations existed between ∆a CEC (post-therapeutic value minus the pre-therapeutic value) and PFS ( P = 0.005) and between ∆ BV and PFS ( P = 0.044); a positive correlation existed between ∆a CEC and ∆ BV. Therefore, both a CEC and tumor BV can serve as predictors, and detection of both indicators can help evaluate the chemo-antiangiogenic efficacy in NSCLC more accurately. [ABSTRACT FROM AUTHOR]

The article discusses a novel single-nucleotide substitution in the erythroid cell-specific regulatory element on B allele in an individual with phenotype B3. ABO blood group typing of a 44-year-old Chinese Han patient was determined using a gel test kit. Particular focus is also given to the importance of correct identification of ABO subgroups in improving the accuracy of blood typing.

The article discusses a novel single-nucleotide substitution in the erythroid cell-specific regulatory element on B allele in an individual with phenotype B3. ABO blood group typing of a 44-year-old Chinese Han patient was determined using a gel test kit. Particular focus is also given to the importance of correct identification of ABO subgroups in improving the accuracy of blood typing.

The article presents a case study of a 34-year-old female patient who showed discrepancy in forward and reverse typying of ABO blood group. Topics discussed include presence of weak B antigens on red blood cells (RBCs), association between B101 allele and c.502C>G single-nucleotide polymorphism and changes in position of ABO protein due to amino acids.