21 results on '"Y. Kawahara"'
Search Results
2. Favorable efficacy of S-1 treatment for locoregionally advanced cutaneous squamous cell carcinoma in the head and neck region.
- Author
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Izumi T, Teramoto Y, Kamimura A, Doi R, Takai S, Mori T, Koizumi S, Kawahara Y, Aitake U, Lei X, Inomata N, Inafuku K, and Nakamura Y
- Subjects
- Humans, Retrospective Studies, Squamous Cell Carcinoma of Head and Neck drug therapy, Neoplasm Recurrence, Local pathology, Carcinoma, Squamous Cell pathology, Skin Neoplasms drug therapy, Head and Neck Neoplasms drug therapy
- Abstract
Cutaneous squamous cell carcinoma is usually treated with surgery; however, locoregionally advanced cutaneous squamous cell carcinoma can be difficult to resect. Although recent guidelines from Western countries recommend using anti-programmed cell death protein 1 (PD-1) antibodies, including cemiplimab and pembrolizumab, there are no approved anti-PD-1 antibodies for locoregional cutaneous squamous cell carcinoma in Asian countries. S-1 is an oral drug with a low incidence of severe toxicity that can be used for head and neck cancers, including head and neck locoregional cutaneous squamous cell carcinoma, in Japan. We retrospectively evaluated patients with head and neck locoregional cutaneous squamous cell carcinoma treated with S-1 at two Japanese institutions (2008-2022). The initial dosage was determined by the body surface area (<1.25 m
2 : 80 mg/day, 1.25-1.5 m2 : 100 mg/day, ≥1.5 m2: 120 mg/day) for 28 consecutive days. The outcome measures were objective response rate (ORR), progression-free survival (PFS), and overall survival (OS). Fourteen patients were included. The ORR was 78%, and the complete response (CR) rate was 64.3%. The median PFS and OS were not reached (NR) (95% confidence interval [CI], 5.9 months-NR) and NR (95% CI, 13.8 months-NR), respectively. The 12-month PFS and OS rates were 51% and 85%, respectively. Six of the nine patients who achieved CR showed no recurrence during the follow-up period (median follow-up, 24.7 months). After CR, three patients experienced recurrence. Among these, two resumed S-1 treatment and subsequently underwent salvage surgery, resulting in a sustained absence of recurrence. One patient developed lung metastasis and died, although S-1 therapy was resumed. Only one patient (7.1%) developed grade 3 anemia. S-1 showed favorable efficacy and low toxicity in patients with head and neck locoregionally advanced cutaneous squamous cell carcinoma. S-1 may be a good alternative to the anti-PD-1 antibody for treating head and neck locoregionally advanced squamous cell carcinoma., (© 2023 Japanese Dermatological Association.)- Published
- 2024
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3. Comparison of surgical morbidities between LigaSure™ and conventional techniques in inguinal or ilioinguinal lymph node dissection for skin cancer: A single center retrospective study.
- Author
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Umeda Y, Teramoto Y, Asami Y, Matsuya T, Saito S, Sasaki K, Baba N, Ishizuki S, Kamimura A, Yamaguchi B, Kawahara Y, Takai S, Izumi T, Doi R, Mori T, and Nakamura Y
- Subjects
- Humans, Lymph Nodes pathology, Lymph Nodes surgery, Lymphatic Metastasis pathology, Morbidity, Retrospective Studies, Lymph Node Excision adverse effects, Lymph Node Excision methods, Skin Neoplasms pathology, Skin Neoplasms surgery
- Abstract
Skin cancer patients with clinical nodal disease or whose positive sentinel nodes had great tumor burden remain candidates for regional lymph node dissections. Among these patients, inguinal or ilioinguinal lymph node dissection is frequently required in clinical practice, which is associated with significant postoperative morbidity-including lymphatic leakage. The aim of this retrospective study was to evaluate the efficacy of LigaSure™, an electrothermal bipolar vessel sealing system, in reducing lymphatic leakage in inguinal or ilioinguinal lymph node dissection. In total, 58 patients who received inguinal or ilioinguinal lymph node dissection (conventional group, 48; LigaSure™ group, 10) and shared similar characteristics were included in this study. Lymphatic leakage after drain removal was significantly lower in the LigaSure™ group than that in the conventional group (present ratio, 0% vs. 37%; p = 0.02). The daily lymphatic drainage volume also tended to be lower in the LigaSure™ than that in the conventional group, with significant differences on postoperative day 1 (p = 0.02). Other perioperative outcomes including the operating time, intraoperative blood loss, time to drain removal, duration of hospital stay, flap necrosis, and wound infection showed no significant differences between the two groups. The use of the LigaSure™ in inguinal or ilioinguinal lymph node dissection for the treatment of skin cancer could reduce the incidence of postoperative lymphatic leakage after drain removal., (© 2022 Japanese Dermatological Association.)
- Published
- 2022
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4. Concordance in judgment of clinical borders of basal cell carcinomas in Japanese patients: A preliminary study of JCOG2005 (J-BASE-MARGIN).
- Author
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Kamimura A, Nakamura Y, Takenouchi T, Matsushita S, Omodaka T, Yamamura K, Uchi H, Yoshikawa S, Yanagisawa H, Ito T, Kiyohara Y, Nakamura Y, Aoki M, Ishizuki S, Oashi K, Miyagawa T, Maeda T, Ogata D, Hatta N, Ohe S, Isei T, Takahashi A, Umeda Y, Yamaguchi B, Ishikawa M, Horimoto K, Fujsawa Y, Uehara J, Shibayama Y, Kiniwa Y, Kawahara Y, Matsuya T, Uhara H, Kato J, Nakamura Y, Murakami T, Namikawa K, Yoshino K, Funakoshi T, Takatsuka S, Matsui Y, Sasaki J, Koga H, Yokota K, Komori T, Fukushima S, and Yamazaki N
- Subjects
- Humans, Japan, Judgment, Margins of Excision, Carcinoma, Basal Cell pathology, Carcinoma, Basal Cell surgery, Head and Neck Neoplasms, Skin Neoplasms diagnosis, Skin Neoplasms pathology, Skin Neoplasms surgery
- Abstract
Basal cell carcinoma is the most common type of skin cancer, and surgical excision with clear margins is the standard of care. Surgical margins are determined based on risk factors (high or low risk) for recurrence according to the National Comprehensive Cancer Network and Japanese basal cell carcinoma guidelines. The clarity of the clinical tumor border (well-defined or poorly defined) is considered a risk factor, and significant discrepancies in the judgment of clinical tumor borders among dermato-oncologists may occur. Therefore, we analyzed the dermato-oncologists' concordance in judging the clinical tumor border of basal cell carcinoma. Forty-seven dermato-oncologists (experts: 37; young trainees: 10) participated in this study. The datasets of clinical and dermoscopic photographs of 79 Japanese cases of head and neck basal cell carcinoma were used to determine the concordance in the judgment of clinical tumor border. The probability of the border that was selected more often was used to calculate the rater agreement rate for each dataset. Correct judgment was defined as a more frequently selected border, and the concordance rate of clarity of clinical tumor border for each dermato-oncologist was calculated based on the definition of the correct judgment. A median concordance rate of 85% or higher for all dermato-oncologists was predefined as an acceptable rate for clinical use. Of the 79 datasets, rater agreement rates were 80-100%, 60-79%, and 51-59% for 55, 19, and five datasets, respectively. The median concordance rate for all dermato-oncologists was 86% (interquartile range: 82-89%). There was no significant difference in the concordance rate between the experts and the trainees (median, 87% vs. 85.5%; p = 0.58). The concordance rates of dermato-oncologists for all datasets were relatively high and acceptable for clinical use., (© 2022 Japanese Dermatological Association.)
- Published
- 2022
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5. Prognostic factors of children and adolescents with T-cell acute lymphoblastic leukemia after allogeneic transplantation.
- Author
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Ishida H, Kato M, Kawahara Y, Ishimaru S, Najima Y, Kako S, Sato M, Hiwatari M, Noguchi M, Kato K, Koh K, Okada K, Iwasaki F, Kobayashi R, Igarashi S, Saito S, Takahashi Y, Sato A, Tanaka J, Hashii Y, Atsuta Y, Sakaguchi H, and Imamura T
- Subjects
- Adolescent, Adult, Child, Humans, Prognosis, Recurrence, Retrospective Studies, T-Lymphocytes, Transplantation Conditioning methods, Transplantation, Homologous, Young Adult, Graft vs Host Disease complications, Hematopoietic Stem Cell Transplantation methods, Precursor Cell Lymphoblastic Leukemia-Lymphoma etiology, Precursor Cell Lymphoblastic Leukemia-Lymphoma therapy, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma therapy
- Abstract
Acute lymphoblastic leukemia (ALL) is the most common cancer during childhood, and some high-risk patients with ALL require hematopoietic stem cell transplantation (HSCT). Mainly due to small patient numbers, studies focusing specifically on children and adolescents with T-cell ALL (T-ALL) are limited. Using a nationwide registry, we retrospectively analyzed data from patients under 20 years old who underwent their first HSCT for T-ALL between 2000 and 2018. As a result, total 484 patients were included, and their median follow-up period was 6.9 years after HSCT for survivors. While patients receiving HSCT at first complete remission (CR) showed relatively good 5-year leukemia free survival (5yLFS, 73.5%), once relapse occurred, their prognosis was much worse (44.4%) even if they attained second remission again (p < 0.001). Among patients receiving HSCT at CR1, grade II-IV acute graft versus host disease was associated with worse overall and LFS than grade 0-I (5yLFS 69.5% vs. 82.1%, p = 0.026) mainly due to high non-relapse mortality. Among those patients, patients receiving related bone marrow transplantation, unrelated bone marrow transplantation, or unrelated cord blood transplantation showed similar survival (5yLFS, 73.2%, 76.3%, and 77.0%, respectively). For patients undergoing cord blood transplantation at CR1, total-body irradiation-based myeloablative conditioning was associated with better 5yLFS than other conditioning regimens (85.4% vs. 62.2%, p = 0.044), as it reduced the risk of relapse. These results indicate that relapsed patients have much less chance of cure, and that identifying patients who require HSCT for cure and offering them HSCT with optimal settings during CR1 are crucial for children and adolescents with T-ALL., (© 2022 John Wiley & Sons Ltd.)
- Published
- 2022
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6. Familial infections caused by nasal colonization of Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus USA300 clone.
- Author
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Ota S, Oka F, Kaneko H, Nakaminami H, and Kawahara Y
- Subjects
- Anti-Bacterial Agents therapeutic use, Bacterial Toxins, Clone Cells, Exotoxins genetics, Humans, Leukocidins genetics, Community-Acquired Infections, Methicillin-Resistant Staphylococcus aureus genetics, Staphylococcal Infections diagnosis, Staphylococcal Infections drug therapy
- Published
- 2022
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7. Improvement of Vascular Endothelial Function Reflects Nonrecurrence After Catheter Ablation for Atrial Fibrillation.
- Author
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Kanazawa H, Kaikita K, Ito M, Kawahara Y, Hoshiyama T, Kanemaru Y, Kiyama T, Iwashita S, Tabata N, Yamanaga K, Fujisue K, Sueta D, Takashio S, Arima Y, Araki S, Usuku H, Nakamura T, Izumiya Y, Sakamoto K, Suzuki S, Yamamoto E, Soejima H, Matsushita K, and Tsujita K
- Subjects
- Humans, Hyperemia, Proportional Hazards Models, Recurrence, Retrospective Studies, Risk Factors, Treatment Outcome, Atrial Fibrillation diagnosis, Atrial Fibrillation surgery, Catheter Ablation, Endothelium, Vascular physiology
- Abstract
Background The clinical implication of vascular endothelial dysfunction in patients with atrial fibrillation (AF) remains unclear. This study aimed to elucidate the correlation between changes in vascular endothelial function assessed by reactive hyperemia-peripheral arterial tonometry and the effect of sinus rhythm restoration after catheter ablation (CA) for AF. Methods and Results Consecutive 214 patients who underwent CA for AF were included in this single center, retrospective study. The natural logarithmic transformed reactive hyperemia-peripheral arterial tonometry index (LnRHI) of all patients was measured before CA as well as 3 and 6 months after CA. LnRHI in sinus rhythm was significantly higher than that in AF before CA. Multivariate logistic regression analysis revealed that the presence of AF was an independent risk factor for lowering of LnRHI (odds ratio, 4.092; P =0.002) before CA. The LnRHI was significantly improved 3 and 6 months after CA in patients without AF recurrence. Multivariate Cox hazard analysis revealed that changes in LnRHI from before to 3 months after CA independently correlated with recurrence of AF (hazard ratio, 0.106; P =0.001). Receiver operating characteristic analysis showed the decrease in LnRHI levels from before to 3 months after CA as a significant marker that suspects AF recurrence (area under the curve, 0.792; log-rank test, P <0.001). Conclusions The presence of AF was independently correlated with the impaired vascular endothelial function assessed by the reactive hyperemia-peripheral arterial tonometry. Long-term sinus rhythm restoration after CA for AF might contribute to the improvement of vascular endothelial function, which may reflect the nonrecurrence of AF.
- Published
- 2021
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8. Long-term resolution of invasive extramammary Paget's disease with multiple regional lymph node metastases solely with regional lymph node dissection.
- Author
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Kawahara Y, Umeda Y, Yamaguchi B, Kamimura A, Teramoto Y, and Nakamura Y
- Subjects
- Humans, Lymph Node Excision, Lymphatic Metastasis, Paget Disease, Extramammary surgery, Skin Neoplasms surgery
- Published
- 2021
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9. Prevalence of germline GATA2 and SAMD9/9L variants in paediatric haematological disorders with monosomy 7.
- Author
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Yoshida M, Tanase-Nakao K, Shima H, Shirai R, Yoshida K, Osumi T, Deguchi T, Mori M, Arakawa Y, Takagi M, Miyamura T, Sakaguchi K, Toyoda H, Ishida H, Sakata N, Imamura T, Kawahara Y, Morimoto A, Koike T, Yagasaki H, Ito S, Tomizawa D, Kiyokawa N, Narumi S, and Kato M
- Subjects
- Adolescent, Child, Child, Preschool, Chromosome Deletion, Chromosomes, Human, Pair 7 genetics, Female, Hematologic Neoplasms epidemiology, High-Throughput Nucleotide Sequencing, Humans, Infant, Infant, Newborn, Male, Myelodysplastic Syndromes epidemiology, Prevalence, GATA2 Transcription Factor genetics, Germ-Line Mutation, Hematologic Neoplasms genetics, Intracellular Signaling Peptides and Proteins genetics, Myelodysplastic Syndromes genetics, Tumor Suppressor Proteins genetics
- Abstract
Monosomy 7 (-7) occurs in various types of paediatric myeloid disorders and has a poor prognosis. Recent studies have demonstrated that patients with germline gain-of-function SAMD9/9L variants and loss-of-function GATA2 variants are prone to developing myelodysplastic syndrome (MDS) associated with -7. However, the prevalence of the genetic variants among paediatric haematologic disorders with -7 is unknown. The present study screened germline variants of GATA2 and SAMD9/9L in 25 patients with various types of paediatric haematological disorders associated with -7. The diagnoses of the 25 patients included MDS (n = 10), acute myeloid leukaemia (AML) and myeloid sarcomas (n = 9), juvenile myelomonocytic leukaemia (n = 3) and other disorders (n = 3). Seven patients with a germline pathogenic GATA2 variant were found. For SAMD9/9L screening, next-generation sequencing was used to detect low-abundance variants and found four novel germline variants. Functional analysis revealed that three out of the four variants showed growth-restricting capacity in vitro and thus, were judged to be pathogenic. Cases with GATA2 mutation tended to be older, compared to those with SAMD9/9L mutations. In conclusion, GATA2 and SAMD9/9L were sequenced in 25 patients with paediatric haematologic disorders associated with -7, and 40% of them were found to have some pathogenic germline variants in the three genes., (© 2020 British Society for Haematology and John Wiley & Sons Ltd.)
- Published
- 2020
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10. Usefulness of 2-D shear wave elastography for the diagnosis of inguinal lymph node metastasis of malignant melanoma and squamous cell carcinoma.
- Author
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Kawahara Y, Togawa Y, Yamamoto Y, Wakabayashi S, Matsue H, and Inafuku K
- Subjects
- Humans, Lymph Nodes diagnostic imaging, Lymphatic Metastasis diagnostic imaging, Carcinoma, Squamous Cell diagnostic imaging, Elasticity Imaging Techniques, Melanoma diagnostic imaging
- Abstract
We used 2-D shear wave elastography to quantify lymph node hardness, from the shear wave velocity, to determine the presence or absence of metastatic lymphadenopathy in the inguinal lymph nodes of five patients with malignant melanoma and squamous cell carcinoma. The shear wave velocity accurately identified all cases of metastasis confirmed by histology, compared with two false-positive and one false-negative finding with positron emission tomography/computed tomography. 2-D shear wave elastography would be useful to evaluate inguinal lymph node metastasis., (© 2020 The Authors. The Journal of Dermatology published by John Wiley & Sons Australia, Ltd on behalf of Japanese Dermatological Association.)
- Published
- 2020
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11. Helicobacter pylori infection modulates endogenous hydrogen sulfide production in gastric cancer AGS cells.
- Author
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Kawahara Y, Hirashita Y, Tamura C, Kudo Y, Sakai K, Togo K, Fukuda K, Matsunari O, Okamoto K, Ogawa R, Mizukami K, Okimoto T, Kodama M, and Murakami K
- Subjects
- Antigens, Bacterial metabolism, Bacterial Proteins metabolism, Cell Line, Tumor, Coculture Techniques, Gastric Mucosa pathology, Humans, Stomach Neoplasms pathology, Gastric Mucosa metabolism, Helicobacter Infections metabolism, Helicobacter pylori metabolism, Hydrogen Sulfide metabolism, Stomach Neoplasms metabolism
- Abstract
Background: Persistent Helicobacter pylori infection induces gastric mucosal atrophy, which is a precancerous condition. Hydrogen sulfide (H
2 S), a gaseous biological transmitter, has been implicated in both the physiological functions of the gastrointestinal tract and its diseases. To understand gastric epithelial cell response against H pylori infection, we investigated the metabolic changes of gastric cancer cells co-cultured with H pylori and observed the modulation of endogenous H2 S production., Materials and Methods: Gastric cancer AGS cells were co-cultured with an H pylori standard strain possessing bacterial virulence factor CagA (ATCC 43504) and a strain without CagA (ATCC 51932). Three hours after inoculation, the cells were subjected to metabolomics analysis using gas chromatography-tandem mass spectrometry (GC-MS/MS). Orthogonal projections to latent structures discriminant analysis (OPLS-DA) and pathway analysis were performed. In addition, intracellular H2 S levels were measured by using HSip-1 fluorescent probe., Results: Results of OPLS-DA showed a significant difference between the metabolism of untreated control cells and cells inoculated with the H pylori strains ATCC 51932 or ATCC 43504, mainly due to 45 metabolites. Pathway analysis with the selected metabolites indicated that methionine metabolism, which is related to H2 S production, was the most frequently altered pathway. H pylori-inoculated cells produced more endogenous H2 S than control cells. Moreover, ATCC 43504-inoculated cells produced less H2 S than ATCC 51932-inoculated cells., Conclusions: H pylori infection modulates endogenous H2 S production in AGS cells, suggesting that H2 S might be one of the bioactive molecules involved in the biological mechanisms of gastric mucosal disease including mucosal atrophy., (© 2020 John Wiley & Sons Ltd.)- Published
- 2020
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12. Glyburide inhibits the bone resorption induced by traumatic occlusion in rats.
- Author
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Arita Y, Yoshinaga Y, Kaneko T, Kawahara Y, Nakamura K, Ohgi K, Arita S, Ryu T, Takase M, and Sakagami R
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- Animals, Inflammasomes, Interleukin-1beta, Male, NLR Family, Pyrin Domain-Containing 3 Protein, RANK Ligand, Rats, Rats, Sprague-Dawley, Bone Resorption prevention & control, Dental Occlusion, Glyburide therapeutic use, Wounds and Injuries drug therapy
- Abstract
Objective: To examine whether glyburide inhibits bone destruction caused by traumatic occlusion in a rat occlusal trauma model., Background: Excessive mechanical stress, such as traumatic occlusion, induces expression of IL-1β and may be involved in bone resorption. NLRP3 inflammasomes have been linked to IL-1β expression, but it is currently unclear whether glyburide, the inhibiter of NLRP3 inflammasome, suppresses occlusal trauma in rats., Methods: Male SD rats aged 7 weeks were used. In the trauma group, the occlusal surface of the maxillary first right molar was raised by attaching a metal wire to apply occlusal trauma to the mandibular first right molar. In the trauma + glyburide group, the NLRP3 inhibitor glyburide was administered orally every 24 hours from 1 day before induction of occlusal trauma. Rats were euthanized after 5 or 10 days, and the maxillary first molars were harvested with the adjacent tissues for histopathological investigation. Immunohistochemical expression of IL-1β, NLRP3, and RANKL was also assessed., Results: On day 5, bone resorption was significantly greater in the trauma group compared with the control group or the trauma + glyburide group, and there were significantly higher numbers of osteoclasts and cells positive for IL-1β, NLRP3, and RANKL in the trauma group., Conclusion: In this study, glyburide inhibits bone resorption by traumatic occlusion in rats. It suggests that the NLRP3/IL-1β pathway might be associated with bone resorption induced by traumatic occlusion., (© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2020
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13. Multiple ulcers on the face due to infection after thread-lifting.
- Author
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Kasai H, Yashiro K, and Kawahara Y
- Subjects
- Acinetobacter Infections microbiology, Actinomycosis diagnosis, Dental Fistula diagnosis, Diagnosis, Differential, Face, Female, Humans, Middle Aged, Rhytidoplasty methods, Skin Ulcer microbiology, Sporotrichosis diagnosis, Acinetobacter isolation & purification, Acinetobacter Infections diagnosis, Rhytidoplasty adverse effects, Skin Ulcer diagnosis
- Published
- 2018
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14. Pediatric case of microscopic polyangiitis with skin manifestations resembling vesiculobullous type erythema elevatum diutinum with immunoglobulin A antineutrophil cytoplasmic antibody.
- Author
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Adachi A, Komine M, Murata S, Tsuda H, Kawahara Y, Morimoto A, and Ohtsuki M
- Subjects
- Child, Diagnosis, Differential, Female, Humans, Microscopic Polyangiitis immunology, Vasculitis, Leukocytoclastic, Cutaneous immunology, Antibodies, Antineutrophil Cytoplasmic, Immunoglobulin A immunology, Microscopic Polyangiitis diagnosis, Vasculitis, Leukocytoclastic, Cutaneous diagnosis
- Published
- 2016
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15. Tissue- and plasma-specific MicroRNA signatures for atherosclerotic abdominal aortic aneurysm.
- Author
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Kin K, Miyagawa S, Fukushima S, Shirakawa Y, Torikai K, Shimamura K, Daimon T, Kawahara Y, Kuratani T, and Sawa Y
- Subjects
- Aged, Aortic Aneurysm, Abdominal blood, Atherosclerosis blood, Biomarkers blood, Biomarkers metabolism, Female, Humans, Male, MicroRNAs blood, Microarray Analysis, Middle Aged, Aortic Aneurysm, Abdominal metabolism, Atherosclerosis metabolism, MicroRNAs metabolism
- Abstract
Background: Atherosclerotic abdominal aortic aneurysm (AAA) is a progressive, gradual aortic rupture that results in death in the absence of surgical intervention. Key factors that regulate initiation and progression of AAA are unknown, making targeted interventions difficult. MicroRNAs play a fundamental role in atherosclerosis, and atherosclerotic coronary artery disease is characterized by tissue- and plasma-specific microRNA signatures. However, little is known about microRNAs involved in AAA pathology. This study examined tissue and plasma microRNAs specifically associated with AAA., Methods and Results: AAA and normal wall tissues were sampled from patients undergoing AAA repair (n=13; mean age, 68±6 years) and aortic valve replacement surgery (n=7; mean age, 66±4 years), respectively. MicroRNA expression was assessed by high-throughput microRNA arrays and validated by real-time polymerase chain reaction for individual microRNAs that showed significant expression differences in the initial screening. MicroRNAs related to fibrosis (miR-29b), inflammation (miR-124a, miR-146a, miR-155, and miR-223), and endothelium (miR-126, let-7 family members, and miR-21) were significantly upregulated in AAA tissue. Significant negative correlations were seen in expression levels of monocyte chemoattractant protein-1 and miR-124a, -146a, and -223; tumor necrosis factor-α and miR-126 and -223; and transforming growth factor-β and miR-146a. Expression of microRNAs, such as miR-29b, miR-124a, miR-155, and miR-223, that were upregulated in AAA tissue was significantly reduced in plasma of patients with AAA (n=23; mean age, 72±9 years) compared to healthy controls (n=12; mean age, 51±11 years) and patients with coronary artery disease (n=17; mean age, 71±9 years)., Conclusions: The expression of some microRNAs was specifically upregulated in AAA tissue, warranting further studies on the microRNA function in AAA pathogenesis and on the possibility of using a microRNA biomarker for AAA diagnosis.
- Published
- 2012
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16. Helicobacter pylori eradication reduced the incidence of gastric cancer, especially of the intestinal type.
- Author
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Takenaka R, Okada H, Kato J, Makidono C, Hori S, Kawahara Y, Miyoshi M, Yumoto E, Imagawa A, Toyokawa T, Sakaguchi K, and Shiratori Y
- Subjects
- Aged, Disease-Free Survival, Drug Therapy, Combination, Female, Helicobacter Infections mortality, Humans, Incidence, Japan epidemiology, Male, Middle Aged, Retrospective Studies, Stomach Neoplasms mortality, Helicobacter Infections prevention & control, Helicobacter pylori, Stomach Neoplasms microbiology
- Abstract
Background: Although Helicobacter pylori infection is closely associated with gastric cancer development, follow-up studies after H. pylori eradication are still scarce., Aim: To clarify the cancer preventive effect of H. pylori eradication, with special attention to differences in effect according to histology., Methods: Patients who underwent H. pylori eradication therapy and were followed-up endoscopically for at least 1 year were analysed. The incidence of gastric cancer and factors associated with cancer development were investigated., Results: A total of 1807 patients were enrolled. Six of 1519 H. pylori eradicated and five of 288 persistent subjects developed gastric cancer. Four of the eradicated subjects developed the intestinal type and two the diffuse type, while four of the persistent subjects developed the intestinal type and one the diffuse type. Kaplan-Meier analysis indicated a significantly lower incidence in eradicated patients than in persistent patients. The incidence of intestinal type was significantly lower than in eradicated patients, while the diffuse type could not be evaluated because of the low incidence., Conclusions: Helicobacter pylori-eradicated patients had a reduced incidence of gastric cancer compared with H. pylori-persistent patients, particularly the intestinal type, suggesting that H. pylori is strongly associated with intestinal-type gastric cancer.
- Published
- 2007
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17. Effect of CYP2C19 polymorphism on the safety and efficacy of omeprazole in Japanese patients with recurrent reflux oesophagitis.
- Author
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Ohkusa T, Maekawa T, Arakawa T, Nakajima M, Fujimoto K, Hoshino E, Mitachi Y, Hamada S, Mine T, Kawahara Y, Nagai T, Aoyama N, Yoshida N, Tadokoro K, Chida N, Konda Y, Seno H, Shimatani T, Inoue M, and Sato N
- Subjects
- Adult, Aged, Anti-Ulcer Agents adverse effects, Anti-Ulcer Agents metabolism, Cytochrome P-450 CYP2C19, Esophagitis, Peptic drug therapy, Esophagitis, Peptic metabolism, Female, Gastrins blood, Gastroesophageal Reflux drug therapy, Gastroesophageal Reflux metabolism, Genotype, Heterozygote, Homozygote, Humans, Male, Middle Aged, Omeprazole adverse effects, Omeprazole metabolism, Recurrence, Treatment Outcome, Anti-Ulcer Agents administration & dosage, Aryl Hydrocarbon Hydroxylases genetics, Esophagitis, Peptic genetics, Gastroesophageal Reflux genetics, Mixed Function Oxygenases genetics, Omeprazole administration & dosage, Polymorphism, Genetic genetics
- Abstract
Background: The polymorphic enzyme cytochrome P450 2C19 affects omeprazole metabolism. This influence on metabolism might affect serum gastrin levels, and safety, during long-term treatment of reflux oesophagitis., Aim: To examine the relationship between cytochrome P450 2C19 genotype and the safety profile of long-term omeprazole treatment., Methods: A total of 119 Japanese patients with recurrent reflux oesophagitis underwent cytochrome P450 2C19 genotyping prior to receiving daily omeprazole 10 mg or 20 mg for 6-12 months, during which adverse event frequency, serum gastrin levels and endoscopic findings were monitored., Results: The incidences of adverse events, serious adverse events and adverse events leading to withdrawal did not differ between homozygous extensive metabolizer (n = 46), heterozygous extensive metabolizer (n = 53) or poor metabolizer (n = 20) groups. In all genotype groups, serum gastrin increased during the first 3 months of dosing but stabilized thereafter. No significant differences were seen either in the rate of reflux oesophagitis healing or symptom improvement among genotype groups., Conclusions: Long-term treatment with omeprazole was well-tolerated in Japanese patients, irrespective of their cytochrome P450 2C19 metabolic genotype, indicating that dose adjustment depending on metabolic genotype is not required during treatment with omeprazole.
- Published
- 2005
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18. Low editing efficiency of GluR2 mRNA is associated with a low relative abundance of ADAR2 mRNA in white matter of normal human brain.
- Author
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Kawahara Y, Ito K, Sun H, Kanazawa I, and Kwak S
- Subjects
- Adenosine Deaminase genetics, Adult, Aged, Female, Humans, Male, Middle Aged, RNA, Messenger genetics, RNA-Binding Proteins, Receptors, AMPA genetics, Receptors, Kainic Acid biosynthesis, Receptors, Kainic Acid genetics, Reverse Transcriptase Polymerase Chain Reaction, Spinal Cord drug effects, Spinal Cord metabolism, GluK2 Kainate Receptor, Adenosine Deaminase biosynthesis, Brain Chemistry physiology, RNA Editing physiology, RNA, Messenger biosynthesis, Receptors, AMPA biosynthesis
- Abstract
The ionotropic glutamate receptor (GluR) subunits GluR2, GluR5 and GluR6 are subject to RNA editing at their Q/R sites, resulting in significant alterations in the channel properties of the receptors. RNA editing at the Q/R site of GluRs is both developmentally and regionally regulated. Here we provide the first quantitative measurements of both mRNAs of the GluR subunits and mRNAs of the RNA editing enzymes ADAR1-ADAR3 in a comparison of the efficiency of editing at the Q/R site with the expression levels of ADAR mRNA in human brain. We demonstrate that the Q/R site of GluRs in white matter is edited significantly less than in grey matter. In addition, by means of quantitative reverse transcription-polymerase chain reaction methods, we demonstrate that the relative abundance of ADAR2 mRNA to GluR2 mRNA is significantly lower in white matter than in grey matter and that the GluR2 Q/R site editing decreased only when the ratio of ADAR2 mRNA (not that of ADAR1 mRNA) to GluR2 mRNA dropped below a threshold (20 x 10(-3)). These results suggest that Q/R site of GluRs editing is regulated in a regional, and hence presumably cell-specific, manner and that the GluR2 Q/R site editing is critically regulated by ADAR2 in human brain.
- Published
- 2003
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19. Spectrum and clinical significance of autoantibodies against transfer RNA.
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Ohosone Y, Ishida M, Takahashi Y, Matsumura M, Hirakata M, Kawahara Y, Nishikawa T, and Mimori T
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- Adult, Aged, Aged, 80 and over, Erythema Multiforme immunology, Female, HeLa Cells, Humans, Immunoblotting, Male, Middle Aged, Precipitin Tests, RNA analysis, RNA isolation & purification, Ribonucleoproteins immunology, Antibodies, Antinuclear analysis, RNA, Transfer immunology, Rheumatic Diseases immunology
- Abstract
Objective: To characterize the clinical features of patients who have autoantibodies against transfer RNA (tRNA) or tRNA-associated proteins., Methods: Sera from 1,472 patients with suspected systemic rheumatic disease were screened by RNA immunoprecipitation of HeLa cell extracts. The specificities of the antibodies that precipitated tRNAs were further analyzed by immunoprecipitation using deproteinized RNAs and 35S-methionine-labeled HeLa cell extracts, followed by immunoblotting., Results: Forty-one serum samples (2.8%) were found to immunoprecipitate tRNAs. Thirteen patients were identified as having previously defined anti-aminoacyl-tRNA synthetase antibodies (anti-histidyl-tRNA synthetase in 4 patients, anti-threonyl-tRNA synthetase in 1, anti-alanyl-tRNA synthetase in 3, anti-glycyl-tRNA synthetase in 4, and anti-isoleucyl-tRNA synthetase in 1). All 13 patients had myositis and/or interstitial pneumonitis. Sera from the remaining 28 patients immunoprecipitated previously unidentified tRNAs, including 13 serum samples that bound deproteinized cognate tRNA; 24 of the 28 patients met criteria for either systemic lupus erythematosus (SLE) or Sjögren's syndrome (SS). In addition, nonerosive polyarthritis, leukocytopenia, rheumatoid factor, and characteristic annular or papulosquamous recurrent erythema were noted in these patients; however, renal involvement was rare. Sera from 16 of these 28 patients also contained anti-Ro/SSA and/or anti-La/SSB antibodies. While 189 patient sera precipitated Ro/SSA and/or La/SSB-associated RNAs but not tRNA, only 12 of the patients (6.3%) developed skin lesions (P=0.0009, odds ratio 8.85)., Conclusion: Novel autoantibodies against tRNAs or tRNA-associated proteins were identified in 28 sera. These autoantibodies appear to be distinct from anti-aminoacyl-tRNA synthetase antibodies and are associated with SLE and SS. The presence of anti-Ro/SSA and/or anti-La/SSB along with anti-tRNA antibodies is more strongly associated with recurrent erythema than is the presence of anti-Ro/SSA or anti-La/SSB alone.
- Published
- 1998
- Full Text
- View/download PDF
20. Two cases of atypical bullous disease showing linear IgG and IgA deposition in the basement membrane zone.
- Author
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Kawahara Y, Hashimoto T, Watanabe K, Kurihara S, Matsuo I, and Nishikawa T
- Subjects
- Adult, Basement Membrane immunology, Humans, Male, Immunoglobulin A metabolism, Immunoglobulin G metabolism, Skin immunology, Skin Diseases, Vesiculobullous immunology
- Abstract
Patients showing coexistent linear IgG and IgA deposition along the basement membrane zone on direct immunofluorescence have been described as either bullous pemphigoid, epidermolysis bullosa acquisita, linear IgA bullous dermatosis, or cicatricial pemphigoid, depending on the clinical features and laboratory findings. In the present report, we describe two cases showing atypical clinical features distinct from those of other known bullous diseases. No circulating antibodies were detected by indirect immunofluorescence of normal human skin. Indirect immunofluorescence of 1 M NaCl split skin revealed IgG and/or IgA antibodies reactive with the dermal side of the split. Immunoblotting of normal human epidermal and dermal extracts showed no apparent reactivity with known autoantigens. The results suggest that there may be a unique and distinct bullous disease with linear IgG and IgA deposition at the basement membrane zone.
- Published
- 1996
- Full Text
- View/download PDF
21. Suicides in active-duty enlisted Navy personnel.
- Author
-
Kawahara Y and Palinkas LA
- Subjects
- Adolescent, Adult, Age Factors, Humans, Interpersonal Relations, Male, Mental Disorders psychology, Racial Groups, Risk Factors, Suicide, Attempted prevention & control, Suicide, Attempted statistics & numerical data, United States, Suicide Prevention, Military Personnel, Suicide statistics & numerical data
- Abstract
Cases of completed suicide among U.S. Navy enlisted personnel occurring from 1974 through 1985 were identified and compared with similar rates in the U.S. general population and in the U.S. Army. Although the Navy suicide rate was the lowest of the three groups, this rate increased between 1976 and 1983. This was in contrast to national and Army trends during the same period. Young white males in the apprentice/recruit and blue-collar occupations had the highest rates of completed suicide in the Navy. Recommendations for prevention and treatment are discussed.
- Published
- 1991
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