1. Suitability of macrophage inflammatory protein-1β production by THP-1 cells in differentiating skin sensitizers from irritant chemicals.
- Author
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Yeon-Mi Lim, Seong-Joon Moon, Su-Sun An, Soo-Jin Lee, Seo-Young Kim, Ih-Seop Chang, Kui-Lea Park, Hyoung-Ah Kim, and Yong Heo
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CHEMICAL terrorism , *CHEMICALS , *INORGANIC chemistry , *CELL proliferation , *CELL growth , *ORGANIC compounds - Abstract
Background: Worldwide restrictions in animal use for research have driven efforts to develop alternative methods. Objective: The study aimed to test the efficacy of the macrophage inflammatory protein-1β (MIP-1β) assay for testing chemicals’ skin-sensitizing capacity. Methods: The assay was performed using 9 chemicals judged to be sensitizing and 7 non-sensitizing by the standard in vivo assays. THP-1 cells were cultured in the presence or absence of 4 doses, 0.01x, 0.1x, 0.5x, or 1x IC50 (50% inhibitory concentration for THP-1 cell proliferation) of these chemicals for 24 hr, and the MIP-1β level in the supernatants was determined. Skin sensitization by the test chemicals was determined by MIP-1β production rates. The MIP-1β production rate was expressed as the relative increase in MIP-1β production in response to chemical treatment compared with vehicle treatment. Results and Conclusion: When the threshold MIP-1β production rate used was 100% or 105% of dimethyl sulfoxide, all the sensitizing chemicals tested (dinitrochlorobenzene, hexyl cinnamic aldehyde, eugenol, hydroquinone, dinitrofluorobenzene, benzocaine, nickel, chromium, and 5-chloro-2-methyl-4-isothiazolin-3-one) were positive, and all the non-sensitizing chemicals (methyl salicylate, benzalkonium chloride, lactic acid, isopropanol, and salicylic acid), with the exception of sodium lauryl sulfate, were negative for MIP-1β production. These results indicate that MIP-1β could be a biomarker for classification of chemicals as sensitizers or non-sensitizers. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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