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Self‐propelling active particles are an exciting and interdisciplinary emerging area of research with projected biomedical and environmental applications. Due to their autonomous motion, control over these active particles that are free to travel along individual trajectories, is challenging. This work uses optically patterned electrodes on a photoconductive substrate using a digital micromirror device (DMD) to dynamically control the region of movement of self‐propelling particles (i.e., metallo‐dielectric Janus particles (JPs)). This extends previous studies where only a passive micromotor is optoelectronically manipulated with a translocating optical pattern that illuminates the particle. In contrast, the current system uses the optically patterned electrode merely to define the region within which the JPs moved autonomously. Interestingly, the JPs avoid crossing the optical region's edge, which enables constraint of the area of motion and to dynamically shape the JP trajectory. Using the DMD system to simultaneously manipulate several JPs enables to self‐assemble the JPs into stable active structures (JPs ring) with precise control over the number of participating JPs and passive particles. Since the optoelectronic system is amenable to closed‐loop operation using real‐time image analysis, it enables exploitation of these active particles as active microrobots that can be operated in a programmable and parallelized manner. [ABSTRACT FROM AUTHOR]

Electrically powered micro- and nanomotors are promising tools for in vitro single-cell analysis. In particular, single cells can be trapped, transported, and electroporated by a Janus particle (JP) using an externally applied electric field. However, while dielectrophoretic (DEP)-based cargo manipulation can be achieved at high-solution conductivity, electrical propulsion of these micromotors becomes ineffective at solution conductivities exceeding "0.3 mS cm-1. Here, JP cargo manipulation and transport capabilities to conductive near-physiological (<6 mS cm-1) solutions are extended successfully by combining magnetic field-based micromotor propulsion and navigation with DEP-based manipulation of various synthetic and biological cargos. Combination of a rotating magnetic field and electric field results in enhanced micromotor mobility and steering control through tuning of the electric field frequency. In addition, the micromotor's ability of identifying apoptotic cell among viable and necrotic cells based on their dielectrophoretic difference is demonstrated, thus, enabling to analyze the apoptotic status in the single-cell samples for drug discovery, cell therapeutics, and immunotherapy. The ability to trap and transport live cells towards regions containing doxorubicin-loaded liposomes is also demonstrated. This hybrid micromotor approach for label-free trapping, transporting, and sensing of selected cells within conductive solutions opens new opportunities in drug delivery and single-cell analysis, where close-to-physiological media conditions are necessary. [ABSTRACT FROM AUTHOR]

Abstract: A class of supercolloidal particles that controllably spin about their central axis in AC electric fields is reported. The rational design of these “microspinners” enables their rotation in a switchable manner, which gives rise to several interesting and programmable behaviors. It is shown that due to their complex shape and discrete metallic patches on their surfaces, these microspinners convert electrical energy into active motion via the interplay of four mechanisms at different electric field frequency ranges. These mechanisms of rotation include (in order of increasing frequency): electrohydrodynamic flows, reversed electrohydrodynamic flows, induced charge electrophoresis, and self‐dielectrophoresis. As the primary mechanism powering their motion transitions from one phenomenon to the next, these microspinners display three directional spin inversions (i.e., from clockwise to anticlockwise, or vice versa). To understand the mechanisms involved, this experimental study is coupled with scaling analyses. Due to their frequency‐switchable rotation, these microspinners have potential for applications such as interlocking gears in colloidal micromachines. Moreover, the principles used to power their switchable motion can be extended to design other types of supercolloidal particles that harvest electrical energy for motion via multiple electrokinetic mechanisms. [ABSTRACT FROM AUTHOR]