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Introduction: Job burnout is an occupational psychological syndrome with a high prevalence among nurses in China. Hemodialysis (HD) nursing work has the characteristics of high intensity, high technical content, and high risk. The aims of this study were to investigate the prevalence and level of job burnout among HD nurses in China and explore the potential factors associated with burnout among HD nurses. Methods: This was a cross‐sectional study in 2019. Survey data were collected from 2738 HD centers in mainland China. Job burnout was measured by the Chinese version of the Maslach Burnout Inventory. The working atmosphere, interpersonal relationships with colleagues, and intention to leave were each assessed by a single question respectively. Multiple linear regression and structural equation modeling were used for the analysis. Findings: A total of 10,570 surveys were collected. A total of 1199 (11.34%) HD nurses reported a high level of emotional exhaustion, 782 (7.40%) reported a high level of depersonalization, and 6767 (64.02%) reported a low level of personal accomplishment. Job burnout in the Northeastern region of mainland China was higher than that in other regions (p < 0.05). The working atmosphere, interpersonal relationships, region, hospital level, educational level, career planning, age, and number of children were significantly associated with burnout among HD nurses (p < 0.001, adjusted R2 = 0.313). The working environment, individual factors, and specialist nurse training were significantly associated with HD nurse burnout and intention to leave (comparative fit index = 0.907; goodness of fit index = 0.930; root mean square error of approximation = 0.055). Discussion: There were notable regional differences in the burnout of HD nurses. This study contributes to the knowledge of the possible relationship of job burnout and intention to leave in HD nurses. It is suggested that improving the working atmosphere or interpersonal relationships and providing more training opportunities can alleviate job burnout in HD nurses. [ABSTRACT FROM AUTHOR]

Background Currently, several diagnostic assays for Helicobacter pylori ( H. pylori) are available, but each has some limitations. Further, a high-flux quantitative assay is required to assist clinical diagnosis and monitor the effectiveness of therapy and novel vaccine candidates. Methods Three hundred and eighty-seven adult patients [nonulcer dyspepsia ( NUD) 295, peptic ulcer disease ( PUD) 77, gastric cancer ( GC) 15] were enrolled for gastrointestinal endoscopies. Three biopsy samples from gastric antrum were collected for the following tests: culture, rapid urease test ( RUT), histopathology, conventional polymerase chain reaction ( PCR), and Multiple Genetic Analysis System ( MGAS). The diagnostic capability of H. pylori for all methods was evaluated through the receiver operating characteristic ( ROC) curves. Results Based on the gold standard, the sensitivity and specificity of MGAS were 92.9 and 92.4%, and positive predict value ( PPV) and negative predict value ( NPV) were 96.0 and 87.1%, respectively. All the above parameters of MGAS were higher than that of culture (except its specificity), RUT and histopathology, and nearly closed to that of conventional PCR. The area under curve ( AUC) was 0.7575 (Culture), 0.8870 ( RUT), 0.9000 (Histopathology), 0.9496 (Conventional PCR), and 0.9277 ( MGAS). No significant statistical difference was observed for the H. pylori DNA load in different disease groups ( p = .067). In contrast, a statistically significant difference in the H. pylori DNA copy number was observed based on age ( p = .043) and gender ( p = .021). Conclusions The data showed that MGAS performed well in detecting H. pylori infection. Furthermore, the quantitative analysis showed that the load of H. pylori was significantly different within both age and gender groups. These results suggested that MGAS could be a potential alternative method for clinical detection and monitoring of the effectiveness of H. pylori therapy. [ABSTRACT FROM AUTHOR]

A correction to the article "A Creative Helicobacter pylori Diagnosis Scheme Based on Multiple Genetic Analysis System: Qualification and Quantitation" is presented.

The recognition of protospacer adjacent motif (PAM) is a key factor for the CRISPR (i.e. clustered regularly interspaced short palindromic repeats)/CRISPR‐associated 9 (Cas9) system to distinguish foreign DNAs from the host genome, and also significantly restricts the targeting scope of the system during genome‐editing applications. Structurally, the PAM interacting (PI) domain, which usually is located in the C‐terminus of Cas9 proteins, directly binds to PAM and plays a key role in determining the recognition specificity. However, several lines of evidence showed that other regions of Cas9 protein beyond the PI domain might also play roles in PAM interaction. Here, we constructed a mosaic SpCas9 protein (xCas9‐NG) by fusing the PI domain of SpCas9 PAM variant, Cas9‐NG with the non‐PI fragment of xCas9 protein that contains multiple amino acid substitutions. We found that non‐PI fragment of xCas9 expanded PAM recognition of the Cas9‐NG PI domain. In addition, xCas9‐NG showed an improved editing efficiency in the majority of targets harboring xCas9 and Cas9‐NG PAMs. Importantly, this finding was also successfully extended to other Cas9 variants, including SpRY and the non‐G SpCas9 series. Together, our work expands the target scope of SpCas9 editing system and demonstrates the notion that the non‐PI domain fragment plays an important role in PAM restriction. [ABSTRACT FROM AUTHOR]