1. Dendritic cells of the mouse recognized by two monoclonal antibodies.
- Author
-
Breel M, Mebius RE, and Kraal G
- Subjects
- Animals, Langerhans Cells immunology, Lymphoid Tissue cytology, Lymphoid Tissue immunology, Mice, Mice, Inbred C3H immunology, Mice, Inbred DBA immunology, Organ Specificity, Rats, Rats, Inbred Strains immunology, Skin cytology, Skin immunology, Antibodies, Monoclonal immunology, Dendritic Cells immunology
- Abstract
A new monoclonal antibody, MIDC-8, is described which shows a comparable tissue distribution as the recently described NLDC-145 antibody. It recognizes interdigitating cells, veiled cells and Langerhans cells in lymphoid organs and the skin of the mouse. In contrast to NLDC-145 it recognizes a cytoplasmic component of these cell types. Its distribution is more restricted to the T cell-dependent areas than NLDC-145. Isolation of dendritic cells from lymph nodes, spleen and thymus revealed that both antibodies react with the in vitro isolated dendritic cells. The results show that these antibodies can be used to study dendritic cells in vitro and emphasize the relationship between the in vivo interdigitating cells of the T cell areas and the in vitro isolated dendritic cells.
- Published
- 1987
- Full Text
- View/download PDF