1. Screening anti-anaphylactoid components in Polygonum cuspidatum via cell membrane chromatography with LC-MS targeting Mas-related G protein-coupled receptor X2.
- Author
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Lin Y, Lu H, Jia Q, and Han S
- Subjects
- Humans, Mass Spectrometry, Cell Membrane drug effects, Cell Membrane metabolism, Cell Membrane chemistry, Chromatography, Liquid, Nerve Tissue Proteins metabolism, Nerve Tissue Proteins antagonists & inhibitors, Mast Cells drug effects, Mast Cells metabolism, Plant Extracts pharmacology, Plant Extracts chemistry, Glucosides pharmacology, Glucosides chemistry, Glucosides analysis, Molecular Structure, Liquid Chromatography-Mass Spectrometry, Receptors, G-Protein-Coupled metabolism, Fallopia japonica chemistry, Receptors, Neuropeptide metabolism, Receptors, Neuropeptide antagonists & inhibitors
- Abstract
Mas-related G protein-coupled receptor X2 (MrgprX2) is acknowledged as a mast cell-specific receptor, playing a crucial role in orchestrating anaphylactoid responses through mast cell degranulation. It holds promise as a target for regulating allergic and inflammatory diseases mediated by mast cells. Polygonum cuspidatum (PC) has shown notable anti-anaphylactoid effects, while its pharmacologically active components remain unclear. In this study, we successfully utilized MrgprX2 high-expressing cell membrane chromatography (CMC), in conjunction with liquid chromatography-mass spectrometry (LC-MS), to identify active anti-anaphylactoid components in PC. Our study pinpointed polydatin, resveratrol, and emodin-8-O-β-d-glucoside as potential anti-anaphylactoid compounds in PC. Their anti-anaphylactoid activities were evaluated through β-aminohexosidase and histamine release assays, demonstrating a concentration-dependent inhibition for both β-aminohexosidase and histamine release. This approach, integrating MrgprX2 high-expression CMC with LC-MS, proves effective in screening potential anti-anaphylactoid ingredients in natural herbal medicines. The findings from this study illuminated the anti-anaphylactoid properties of specific components in PC and provided an efficient method for the drug development of natural products., (© 2024 Wiley‐VCH GmbH.)
- Published
- 2024
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