1. Effects of Grafting Density and Film Thickness on the Adhesion of Staphylococcus epidermidis to Poly(2-hydroxy ethyl methacrylate) and Poly(poly(ethylene glycol)methacrylate) Brushes.
- Author
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Ibanescu SA, Nowakowska J, Khanna N, Landmann R, and Klok HA
- Subjects
- Biocompatible Materials chemistry, Epoxy Compounds chemistry, Humans, Infections microbiology, Methacrylates chemistry, Methacrylates pharmacology, Polyethylene Glycols chemistry, Polyethylene Glycols pharmacology, Polyhydroxyethyl Methacrylate chemistry, Polyhydroxyethyl Methacrylate pharmacology, Polymerization, Polymers chemistry, Polymers pharmacology, Polymethacrylic Acids, Staphylococcus epidermidis pathogenicity, Surface Properties, Bacterial Adhesion drug effects, Biocompatible Materials pharmacology, Infections drug therapy, Staphylococcus epidermidis drug effects
- Abstract
Thin polymer films that prevent the adhesion of bacteria are of interest as coatings for the development of infection-resistant biomaterials. This study investigates the influence of grafting density and film thickness on the adhesion of Staphylococcus epidermidis to poly(poly(ethylene glycol)methacrylate) (PPEGMA) and poly(2-hydroxyethyl methacrylate) (PHEMA) brushes prepared via surface-initiated atom transfer radical polymerization (SI-ATRP). These brushes are compared with poly(ethylene glycol) (PEG) brushes, which are obtained by grafting PEG onto an epoxide-modified substrate. Except for very low grafting densities (ρ = 1%), crystal violet staining experiments show that the PHEMA and PPEGMA brushes are equally effective as the PEG-modified surfaces in preventing S. epidermis adhesion and do not reveal any significant variations as a function of film thickness or grafting density. These results indicate that brushes generated by SI-ATRP are an attractive alternative to grafted-onto PEG films for the preparation of surface coatings that resist bacterial adhesion., (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2016
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