1. Shellfish Tropomyosin IgE Cross-Reactivity Differs Among Edible Insect Species.
- Author
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Palmer LK, Marsh JT, Lu M, Goodman RE, Zeece MG, and Johnson PE
- Subjects
- Adult, Animals, Cross Reactions, Female, Humans, Immune Sera, Immunoglobulin E metabolism, Insect Proteins immunology, Male, Middle Aged, Shellfish, Shellfish Hypersensitivity immunology, Tandem Mass Spectrometry, Tropomyosin genetics, Edible Insects immunology, Food Hypersensitivity immunology, Immunoglobulin E immunology, Tropomyosin immunology
- Abstract
Scope: Insects are a potentially environmentally friendly alternative dietary protein source to supplement mammalian and fish sources, but potential allergenic risks are a concern. Consumption of insects may result in anaphylaxis and has been implicated in cross-reactivity with shellfish. Many allergenic proteins may be involved in cross-reactivity, including tropomyosin (TM). The uniformity of TM cross-reactivity among edible insects is unknown. Candidate edible insects for variability in shellfish IgE cross-reactivity are investigated., Methods and Results: Selected insects and known related sources of allergens are extracted and probed by immunoblot with sera/plasma from patients sensitized to insects or shellfish. Quantification of TM in these extracts is performed using mass spectrometry. A comparison of the quantity of TM and the IgE reactivity of TM from these insects is performed. Distinct patterns of IgE cross-reactivity are observed with three insect species showing diminished reactivity. This pattern is not consistent with the amount of TM present in these insects, or with overall sequence homology., Conclusion: Insects display a diversity of TM-associated IgE reactivity. It is likely that minor sequence features and/or structural effects are primarily responsible. Additionally, it is demonstrated that some insect species may present significantly less IgE cross-reactivity to shrimp than do others., (© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2020
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