1. Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infection.
- Author
-
Rattanarojpong T, Wang HC, Lo CF, and Flegel TW
- Subjects
- Animals, Gills virology, Penaeidae genetics, Proteome genetics, RNA, Viral genetics, Roniviridae genetics, Transcription, Genetic, Gills metabolism, Penaeidae metabolism, Penaeidae virology, Proteome biosynthesis, RNA Virus Infections metabolism, RNA, Viral biosynthesis, Roniviridae physiology
- Abstract
In this proteomic analysis of gills from yellow head virus (YHV)-infected Penaeus vannamei, we identified 13 spots with up-regulated protein expression levels and five spots with down-regulated levels. LC-nanoESI-MS/MS indicated that the up-regulated proteins included enzymes in the glycolytic pathway, the tricarboxylic acid cycle and amino acid metabolism. The other up-regulated proteins were arginine kinase, imaginal disk growth factor (IDGF) and a Ras-like GTP binding protein. By contrast, expression levels were reduced for an SCP-calcium binding protein (SCP), actin-1, a valosin-containing protein, and Rab11. Time-course assays by real time RT-PCR revealed no significant increase in mRNA level of glycolytic enzymes and arginine kinase. However, a significant decrease in SCP mRNA was observed. The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general.
- Published
- 2007
- Full Text
- View/download PDF