1. Discovery of a Single Monooxygenase that Catalyzes Carbamate Formation and Ring Contraction in the Biosynthesis of the Legonmycins.
- Author
-
Huang S, Tabudravu J, Elsayed SS, Travert J, Peace D, Tong MH, Kyeremeh K, Kelly SM, Trembleau L, Ebel R, Jaspars M, Yu Y, and Deng H
- Subjects
- Biological Products chemistry, Carbamates chemistry, Genes, Bacterial, Mixed Function Oxygenases chemistry, Mixed Function Oxygenases genetics, Multigene Family, Pyrrolizidine Alkaloids chemistry, Soil Microbiology, Streptomyces chemistry, Streptomyces genetics, Streptomyces metabolism, Biological Products metabolism, Carbamates metabolism, Mixed Function Oxygenases metabolism, Pyrrolizidine Alkaloids metabolism, Streptomyces enzymology
- Abstract
Pyrrolizidine alkaloids (PAs) are a group of natural products with important biological activities. The discovery and characterization of the multifunctional FAD-dependent enzyme LgnC is now described. The enzyme is shown to convert indolizidine intermediates into pyrrolizidines through an unusual ring expansion/contraction mechanism, and catalyze the biosynthesis of new bacterial PAs, the so-called legonmycins. By genome-driven analysis, heterologous expression, and gene inactivation, the legonmycins were also shown to originate from non-ribosomal peptide synthetases (NRPSs). The biosynthetic origin of bacterial PAs has thus been disclosed for the first time., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
- Full Text
- View/download PDF