1. Branched-Chain Polyamine Found in Hyperthermophiles Induces Unique Temperature-Dependent Structural Changes in Genome-Size DNA.
- Author
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Nishio T, Yoshikawa Y, Fukuda W, Umezawa N, Higuchi T, Fujiwara S, Imanaka T, and Yoshikawa K
- Subjects
- Animals, Bacteriophage T4 genetics, Cattle, DNA genetics, Genome, Hot Temperature, Microscopy, Atomic Force, Nucleic Acid Conformation, Polyamines chemistry, Spermidine chemical synthesis, Thermococcus chemistry, DNA chemistry, Spermidine analogs & derivatives, Spermidine chemistry
- Abstract
A pentavalent branched-chain polyamine, N
4 -bis(aminopropyl)spermidine 3(3)(3)4, is a unique polycation found in the hyperthermophilic archaeon Thermococcus kodakarensis, which grows at temperatures between 60 and 100 °C. We studied the effects of this branched-chain polyamine on DNA structure at different temperatures up to 80 °C. Atomic force microscopic observation revealed that 3(3)(3)4 induces a mesh-like structure on a large DNA (166 kbp) at 24 °C. With an increase in temperature, DNA molecules tend to unwind, and multiple nano-loops with a diameter of 10-50 nm are generated along the DNA strand at 80 °C. These results were compared to those obtained with linear-chain polyamines, homocaldopentamine 3334 and spermidine, the former of which is a structural isomer of 3(3)(3)4. These specific effects are expected to neatly concern with its role on high-temperature preference in hyperthermophiles., (© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)- Published
- 2018
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