1. Inhibitory effect of the paraoxonase gene on the formation of rabbit coronary atherosclerosis.
- Author
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Bai J, Zhou H, Yang XH, Liu HF, and Meng YY
- Subjects
- Analysis of Variance, Animals, Aryldialkylphosphatase administration & dosage, Aryldialkylphosphatase pharmacology, Cholesterol metabolism, Coronary Artery Disease genetics, Genetic Therapy methods, Injections, Male, Rabbits, Random Allocation, Transfection, Triglycerides metabolism, Aryldialkylphosphatase genetics, Coronary Artery Disease prevention & control
- Abstract
Objective: To observe the effect on the inhibition of coronary atherosclerosis hardening of the paraoxonase gene (PON-1) which transfected to the rabbit epicardial adipose tissue., Methods: Rabbit coronary atherosclerosis model was established by high-fat feeding, liposome-encapsulated recombinant plasmid pEGFP-PON-1 50 μ L was injected to the rabbit pericardial cavity, and was harvested 4 weeks after transfection., Results: The epicardial fat transfected PON-1 gene had effect on the high lipid level. It significantly increased expression of PON-1 in peripheral arterial vascular tissue (P <0.05); and significantly reduced total cholesterol and low-density lipoprotein cholesterol levels (P<0.05), and the thickness ratio of coronary artery intima/media (P <0.05)., Conclusions: The injection of the PON-1 gene in the pericardial cavity can effectively suppress the formation of coronary atherosclerosis., (Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.)
- Published
- 2013
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