1. Sialic acid accelerates the electrophoretic velocity of injured dorsal root ganglion neurons
- Author
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Chen-xu Li, Guo-ying Ma, Min-fang Guo, and Ying Liu
- Subjects
microtubule ,axon ,kinesin-5 ,Eg5 ,regeneration ,monastrol ,molecular motor protein ,aging ,neurodegenerative disorders ,telomere shortening ,MSCs ,cellular therapy ,traumatic brain injury ,spinal cord injuries ,dual diagnosis ,diagnosis ,complications ,rehabilitation ,post-concussion syndrome ,brain concussion ,blood brain barrier ,phage display ,peptide library ,nanocarrier ,targeting ,Schwann cells ,neurite outgrowth ,neuromuscular junction (NMJ) ,multiple sclerosis ,TGF-β/BMP-7/Smad signaling ,myogenic differentiation ,Trf3 ,tumor suppression ,nerve regeneration ,bone marrow mesenchymal stem cells ,cerebral ischemia ,tail vein injection ,middle cerebral artery occlusion ,cell therapy ,neuroprotection ,brain injury ,neuroimaging ,ferumoxytol ,superparamagnetic iron oxide particles ,human adipose-derived stem cells ,intracerebral injection ,magnetic resonance imaging ,enhanced susceptibility-weighted angiography image ,modified neurological severity scores ,rats ,Prussian blue staining ,neural regeneration ,non-invasive brain stimulation ,transcranial magnetic stimulation ,neurotrophic factor ,brain-derived neurotrophic factor ,neuroplasticity ,hippocampus ,cognitive function ,curcumin ,neurons ,HIV-1 gp120 V3 loop ,plasticity ,HIV-associated neurocognitive disorders ,output/input curve ,long-term potentiation ,excitatory postsynaptic potential ,paired-pulse facilitation ,Ca 2+ ,synaptosome ,NSFC grants ,hydrogen sulfide ,cerebral ischemia/reperfusion injury ,P2X 7 receptor ,5-triphenyl-2H-tetrazolium chloride staining ,animal model ,protection ,sodium hydrosulfide ,immunofluorescence ,NSFC grant ,γ-aminobutyric acid ,glial fibrillary acidic protein ,glutamic acid decarboxylase ,neurotoxicity ,weaning ,organ index ,cerebrum ,cortex ,glutamate ,p53 tumor suppressor gene family ,cerebral ischemia/reperfusion ,pyramidal neurons ,CA1 region ,delayed neuronal death ,immunohistochemistry ,western blotting ,spinal cord injury ,rapamycin ,Wnt/β-catenin signaling pathway ,apoptosis ,caspase-3 ,loss of neurons ,hydrogen-rich saline ,reactive oxygen species ,physiological saline ,oxidative stress ,Basso ,Beattie and Bresnahan score ,malondialdehyde ,superoxide dismutase ,calcitonin gene-related peptide ,peripheral nerve injury ,rabbits ,sciatic nerve injury ,autologous nerve repair ,polylactic glycolic acid conduit ,extracellular matrix gel ,grafting ,stress relaxation ,creep ,viscoelasticity ,histomorphology ,electrophysiology ,pain sense model ,dorsal root ganglion ,primary sensory neuron ,glycosylated membrane protein ,sialic acid ,cell electrophoresis ,electrophoresis velocity ,heat-hyperalgesia behavior ,hyperalgesia ,neuraminidase ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Peripheral nerve injury has been shown to result in ectopic spontaneous discharges on soma and injured sites of sensory neurons, thereby inducing neuropathic pain. With the increase of membrane proteins on soma and injured site neurons, the negatively charged sialic acids bind to the external domains of membrane proteins, resulting in an increase of this charge. We therefore speculate that the electrophoretic velocity of injured neurons may be faster than non-injured neurons. The present study established rat models of neuropathic pain via chronic constriction injury. Results of the cell electrophoresis test revealed that the electrophoretic velocity of injured neuronal cells was faster than that of non-injured (control) cells. We then treated cells with divalent cations of Ca 2+ and organic compounds with positive charges, polylysine to counteract the negatively charged sialic acids, or neuraminidase to specifically remove sialic acids from the membrane surface of injured neurons. All three treatments significantly reduced the electrophoretic velocity of injured neuronal cells. These findings suggest that enhanced sialic acids on injured neurons may accelerate the electrophoretic velocity of injured neurons.
- Published
- 2015
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