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In this paper, core–shell structured (ZnSe) is prepared bio synthesis by cold plasma technique under atmospheric pressure with an exposure time of 3 min and a gas flow rate of 3 L/min. Films' structural characteristics and morphological characterization were investigated by X-ray diffractometer, atomic force microscopy (AFM) and scanning electron microscopy (FE-SEM). In addition, parameters like crystal size were calculated. Results showed XRD patterns exhibit structure of polycrystalline of preferential orientation (111) direction. SEM technique shows that the nanoparticles presented are spherical. AFM image verified film-formed spherical particles distribute uniformly. The antibacterial diffusion method property of these nanoparticles was performed against Gram-negative bacteria of Escherichia coli and Gram-positive bacteria of Staphylococcus aureus, showing good control of said bacteria. The maximum level of inhibition was found in coli form bacteria with an average inhibition zone diameter with S. aureus, implying an increasing trend with increasing/decreasing loading volume of NC volume. Therefore, these nanomaterials, which can be prepared in a simple and cost-effective way, may be suitable for new types of germicidal materials. [ABSTRACT FROM AUTHOR]