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[Objectives] This study was conducted to develop a rapid propagation method for red sandalwood ( Ptenxxirpus santalinus ) by tissue culture. [Methods] The well-grown red sandalwood seed embryos were inoculated into three kinds of culture media after aseptic treatment, and the aseptic explants were obtained and inoculated into six kinds of media for light culture. [Results] In the best disinfection schemes of red sandalwood, disinfecting with HgCl2, for 8 min achieved the highest germination and survival rates; when the medium for inducing red sandalwood explants was MS + 0. 2 mg/L IBA, the induction rate reached a maximum value; and when the culture medium for inducing stem segments of aseptic red sandalwood planllets was MS +3. 0 mg/L 6-BA +0. 3 mg/L IBA, the growth of the stem segments achieved llie best effect. 'llie optimal medium for inducing red sandalwood explants was MS +1BA 0.2 mg/L, and the optimal medium for inducing stem segments of red sandalwood was MS +3. 0 mg/L 6-BA +0.3 mg/L IBA. [ Conclusions] 'llie results of this study have a large reproductive coefficient, simple process and low cost, which have outstanding value for promoting the breeding and promotion of red sandalwood seedlings. [ABSTRACT FROM AUTHOR]