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[Objectives] To establish the quality standard of barberry branches. [Methods] Microscopic identification and thin layer identification were used to qualitatively identify barberry branches. Berberine content was determined by HPLC method, and the content of water, total ash, acid insoluble ash and extract was detected according to the method of the Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification showed that the features were obvious, and stone cells, cork cells, epidermal cells, stomata, fibers and catheter with reticulated pores could be found. Berberine was detected in barberry branches by thin layer chromatography, and the characteristic spots were separated clearly. Moisture, total ash, and acid insoluble ash content shall not exceed 13%, 6%, and 3%, respectively, and extract content shall not be less than 10%. Berberine hydrochloride (Cx, H]s C1NO4) should be calculated in the branches of Berberis wilsonae Hemsley and Berberis aggregaia C. K. Schneid., and berberine (C20H1N04) content should not be less than 0.05%. The linear relationship was good in the range of 0.002 - 0.240 mg/mL (R2 =0.999 5). The average recovery was 89.63%, and BSD vias 5.28%. [ Conclusions] The method was simple, accurate and reproducible, and can be used for the quality control of barberry branches. [ABSTRACT FROM AUTHOR]

[Objectives] The paper was to establish the quality standard of Yi herb medicine Millettia dielsiana. [Methods] Yi herb medicine M. dielsiana was qualitatively identified by microscopic identification and thin layer identification. The content of total isoflavones was determined by UV-Vis spectrophotometry, and the contents of moisture, total ash, acid-insoluble ash and extract were checked according to the method introduced in Chinese Pharmacopoeia (2020 edition). [Results] The microscopic identification features, including stone cells, fiber bundles, calcium oxalate square crystals, starch granules, pigment blocks, ducts, etc., were distinct. A TLC method for identification of M. dielsiana was established. The contents of water, total ash, acid-insoluble ash and extract should be no more than 13%, no more than 7%, no more than 0.5% and no less than 6% in 10 batches of samples, respectively. The total isoflavones of M. dielsiana based on genistein (C15H10O5) should be no less than 0.25%. The linear relationship of genistein was good in the range of 0.002 - 0.007 mg/mL (R² = 0.999 6) ; the average recovery was 97.01 % and the RSD was 2. 62%. [Conclusions] The method is simple, accurate and well-reproducible, and can be used for the quality control of Yi herb medicine M. dielsiana. [ABSTRACT FROM AUTHOR]