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[Objectives] This study aimed to evaluate the quality of Bupleurum falcatum Linne interplanted with walnut in Ganluo and study the development and utilization value of other parts. [Methods] The contents of saikosaponins a and d were determined with RP-HPLC, and the contents of 7 kinds of harmful elements were determined by ICP-MS. The quality of B. falcatum Linne was compared with that of other Bupleurum species. [Results] The total saikosaponin content in Radix Bupleurum Falcatum (15.894 mg/g) was higher than that in Radix Bu-pleuri of other origins (8.748 mg/g). The total saikosaponin content in leaves of B. falcatum Linne (7.518 mg/g) is more than twice the limit promulgated by Chinese Pharmacopoeia. The contents of Al, Cr, Cu, As, Cd, Pb and Hg in B. falcatum Linne were all lower than the limits promulgated by the pharmacopoeia. In short, the quality of Radix Bupleurum Falcatum was better than that of Radix Bupleurum Marginatum (S3, S4) and Radix Bupleurum Sachalinense (S5). The leaves of B. falcatum Linne contained more saikosaponins and less harmful elements. [Conclusions] The method and technology of interplanting B. falcatum Linne with walnut in Ganluo are mature. The quality of the medicinal materials produced is superior, and the leaves are also rich in saikosaponins a and d, and can be used as the raw material for extracting saikosaponins a and d. This study provides a basis for further in-depth research on the cultivation of B. falcatum Linne in the domestic market. [ABSTRACT FROM AUTHOR]

[Objectives] TTie research aimed to compare antioxidalion aliilities of different extraction phases of llippophae rhamnoides L. leaves extract and find its active marker. [Methods] H. rhamnoides L. leaves were extracted firstly by 75% of ethanol. and then were extracted by petroleum ether, chloroform, ethyl acetate and n-butanol in turn after decompression concentration. Via DPPH and ABTS radical scavenging experiments and Fe reduction experiment, antioxidation activity of each phase was evaluated. And chemical compositions related to its antioxidalion activity were determined by using UPI.C for qualitative identification. Results Antioxidation activity sequence of each phase was ethyl acetate phase ~n-butanol phase > alcohol extract of raw material > water phase > chloroform phase > petroleum ether phase. 33 kinds of chemical compositions were isolated from alcohol extract and extracts by using UPLC. [ Conclusions UPLC and antioxidation activity test were used simultaneously to analyze alcohol extract and extracts of H. rhamnoides L. leaves, which could fast and accurately determine extraction phase with the strongest antioxidation ability. The research is significant to study active ingredients and mechanism of antioxidation from H. rhamnoides L. leaves. [ABSTRACT FROM AUTHOR]

[Objectives] To determine the content of neoastilbin. asiilbin. neoisoastilbin. isoaslilbin, engeletin. and resveralrol in Smilacis abrae rhizome. Methods] The ultra-high-performance liquid chromatography method with diode array detection (UPLC-DAD) was developed. Analysis conditions as follows; waters Aequity UHPLC HT3 C18 chromatographic column (2.1×100 mm) . column temperature 30°C . mobile phase acetonitrile (A) -0.1% phosphoric acid (B ) gradient elution. flow rate 0. 2 mL/min. and detection wavelength 327 inn.[Results] Results indicated that the content of astilbin. neoaslilbin, isoaslilbin. neoisoastilbin. engeletin. and resveralrol in 10 samples of Smilacis glabrae rhizome was 1.313-6. I755mg, 0.013 -3.699 mg, 0.146-0.975 mg, 0.137 -0.7 mg, 0. 147-0.797 mg. 0.077-0. 112 mg, respectively. [Conclusions] This method is high efficient, convenient and accurate, and can be used as the method for quality control of Smilacis glabrae rhizome. [ABSTRACT FROM AUTHOR]

[Obbective] To determine tracheloside and trachelogenin in Trachelospermumjasminoides (Lindl.) Lem. from different production areas by reverse phase high-performance liquid chromatography (RP-HPLC) method. [Methods] The experiment adopted ODS column (4.6 mm X 250 mm, 5 µm); the mobile phase was methanol-water solution; the volumetric flow rate was 1.0 mL/min; the detection wavelength was 280 nm; the column temperature was 30°. [Results] The highest content of tracheloside came from Chun'an of Zhejiang Province, followed by Tonglu of Zhejiang Province; the highest content of trachelogenin also came from Chun'an of Zhejiang Province, following by Lushan of liangxi Province. [Conclusions] The experiment results provide references for identification and application of Trachelospermumjasminoides (Lindl.) Lem., and the established method is reliable, accurate and highly reproducible, and thus can be rffectively applied in evaluating the quality of Trachelospermum jasminoides (Lindl.) Lem. from difíerent production areas. [ABSTRACT FROM AUTHOR]

[Objective] To determine the content of total caffeoyl quinic acid esters (TCaf) in Erigeron multiradiatus and optimize the extraction process of total caffeoyl quinic acid esters in Erigeron multiradiatus. [Methods] Ultraviolet-Visible Spectrophotometry was used. 1,3-Dicaffeoylquinic acid was used as reference substance to determine the content of total caffeoyl quinic acid esters in Erigeron multiradiatus. Single factor experiment was carried out to study influences of ethanol mass fraction, extraction time, extraction temperature, and solid-to-liquid ratio on extraction rate of total caffeoyl quinic acid esters. And L9(34) orthogonal experiment was carried out to optimize extraction process of total caffeoyl quinic acid esters in Erigeron multiradiatus, and determine content of total caffeoyl quinic acid esters in Erigeron multiradiatus samples. [Results] The optimal extraction process is ethanol volume fraction 55%, extraction temperature 60°C, extraction time 100 min, solid-to-liquid ratio 1:14. Under this condition, the extraction rate of total caffeoyl quinic acid esters in Erigeron multiradiatus is 73.52 mg/g; there is significant difference in the content of total caffeoyl quinic acid esters between different plant positions, and leaves contain the highest total caffeoyl quinic acid esters. [Conclusion] The optimized extraction process is reasonable and feasible and can be used in extraction of total caffeoyl quinic acid esters in Erigeron multiradiatus. [ABSTRACT FROM AUTHOR]

[Objectives] To study the changes in active components before and after microorganism fermentation of Rhizoma Paridis (Chonglou). [Methods] Analytical methods using total saponins and total flavonoids as indicators were established. UV spectrophotometry was used to determine the changes in active components before and after fermentation of Rhizoma Paridis. [Results] The content of total saponins decreased after fermentation, while the content of total flavonoids increased. [Conclusions] The content of total saponins decreased after fermentation and the content of total flavonoids increased. The fermentation process of Monascus purpureus Went. needs further optimization. [ABSTRACT FROM AUTHOR]

[Objectives] The research aimed to identify Paris polyphylla with different growth year numbers and evaluate its resource quality. [Methods] Comparative research on P. polyphylla with different growth year numbers was conducted by using morphological identification and microscopic identification method. [Results] By contrasting original plant morphology, transverse sections of rhizome and fibrous root, powder, it displayed that microstructure of P. polyphylla had regularity difference in cell shape and size, duct quantity, trait and size, size and distribution of calcium oxalate cluster crystal. [Conclusions] The microstructure change of P. polyphylla was related to the number of its growth year, which could provide the reference for identifying the growth year number of P. polyphylla and evaluating its resource quality. [ABSTRACT FROM AUTHOR]

[Objectives] To identify original plant and traits of Yi medicine Mubaribo (Verbenae herba) and provide effective experimental data for its identification and application. [Methods] The original plant, traits, and microscopic identification were used. [Results] Stem was square, the edges of basal leaves usually had rough saw teeth and notches; most cauline leaves were three parted, edges of lobes have uneven saw teeth, both edges had hard hair; spikes were terminal and axiilary, thin and delicate; coroHa was light purple to blue. Epidermal cells of stem were long polygon! or quasi-rectangular, vertical waH was straight with pores; lower epidermal ceHs of leaves were vertid to waH and took on wavy bending; pores were infinitive or anisocytic; 3 - 5 subsidiary ceHs; 4 glandularscale head ceHs, stalk single ceH, non-glandular single ceH; poUen grains were quasi-circular or quasi-triangular, smooth surface, and 3 germination apertures. [Conclusions] Yi medicine Mubaribo has distinctive identifying characteristics in origina plant, traits, and microscopic tissues. This study is expected to provide certain reference for identification of original plant and medicine and formulation of quality standad for Yi medicine Mubaribo, and to provide basis for its further reseach and development. [ABSTRACT FROM AUTHOR]

[Objectives] The aim was to establish a UPLC method for simultaneous determination of two components in Erjing piils and compare the content changes of rutin and chlorogenic acid in the preparing process of Polygonatum sibiricum Delar. as well a optimize the compatibility of Erjing piils. [Methods] The chromatographic separation was carried out at 35°C on an ACQUITY UPLC HSS T3 (100 mm X 2. 1 mm, 1.8 µm) column, the mobile phase was methanol(A) and 0.2% phosphoric acid solution(B), the detective wavelength was 345 nm with the flow rate of 0.4 mL/min. [Results] The contents of rutin and chlorogenic acid in Erjing piils after processing were 2 - 3 times before processing. [Conclusions] The processed mixture of Polygonatum sibiricum Delar. and Lycium barbarum L. is the best, the method is simple, accurate and reproducible. [ABSTRACT FROM AUTHOR]