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Introduction:The presence of microbes in our environment is always a permanent public health problem. In this context, research on natural treatment, less expensive and accessible to fight these microbial germs would be beneficial. Methods:During this work, molecules of thiosemicarbazones due to their numerous biological activities were hemi-synthesizedin situin the essential oil ofCymbopogon citratusin order to evaluate their antimicrobial activities. Results:Analysis of the essential oil extracted by hydrodistillation revealed the presence of 72.91% of citral. Citralthiosemicarbazone (CThio) and citral 4-phenyl-3-thiosemicarbazone (CPthio) were hemi-synthesized in this oil with interesting yields of 83% and 91%, respectively. After purification and confirmation of the structures of these molecules, the three substances were tested on eleven strains of microbes. Determination of the inhibition diameters showed that the activity of the essential oil is best in over 80% of strains. However, the largest diameter of inhibition (26 mm) was noted with CPthio againstSalmonella typhiR 30951401. The determination of the minimum inhibitory concentrations showed that the oil remains more active with the smallest value of 0.3125 mg / ml againstMicrococcus luteus. The lower value of minimum bactericidal concentrations was also obtained with the essential oil againstEnterococcus foecalisATCC 29212. Conclusion:The essential oil ofC. citratusremains more active in the majority cases. It could be a great alternative in the fight against bacteria, and the advantage is that it remains a natural substance.

Mitragyna inermis is a plant widely used by breeders in the control of small ruminants. The objective of this study is to evaluate the anthelmintic properties of the watery triturated extract of the leaves of this plant on Haemonchus contortus, a severe parasite of small ruminants in Benin. The best results are obtained with the inhibition of larval migration and motility of adult worms. The watery triturated extract of the plant ignificantly inhibited larval migration (p < 0.001) and motility of adult worms (p < 0.001) in H. contortus. This justifies these anthelmintic properties in vitro. Phytochemical screening reveals the presence of some secondary metabolites such as: polyphenols, flavonoids and condensed tannins. The toxicity study indicates that the triturated extract is not toxic in vitro on Artemia salina larvae with a lethal concentration greater than 0.1 mg/ml. The success of purification tests of the extract would be more important to characterize the active ingredients for the properties recognized by breeders.