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Objective: To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2017, so as to provide the evidence for formulating the targeted strategy of malaria elimination., Methods: The data of malaria cases in Jiangsu Province in 2016 were collected from China's Routine Diseases Surveillance Information System (CRDSIS)., Results: Totally, 239 imported malaria cases were reported in Jiangsu Province in 2017, and the cases decreased by 22.40% compared to 308 cases in 2015. Except 2 malaria case caused by blood transfusion, the rest patients were all imported. Among them, there were 163 falciparum malaria cases, 21 vivax malaria cases, 11 quartan malaria cases, 43 ovale malaria cases, and 1 mixed infection case ( Plasmodium falciparum and P. ovale ). The numbers of imported cases of Nantong (39 cases, 16.32%), Suzhou (26 cases, 10.88%), Taizhou (25 cases, 10.46%), Huai'an (24 cases, 10.04%), and Lianyungang (22 cases, 9.21%) ranked in the top 5 cities across Jiangsu Province, the malaria cases in the five cities accounted for 56.90% (136/239). The infection source areas of the imported malaria cases included Africa (225 cases), Asia (8 cases), Oceania (2 cases), and South America (2 cases)., Conclusions: Jiangsu Province has no local malaria cases for 6 consecutive years. Despite the imported cases in 2017 decreased some-what compared to that in 2016, it is still necessary to strengthen the surveillance of imported malaria cases and improve malaria diagnosis and treatment in the whole province.

Recently, the global malaria control has achieved remarkable results, and the epidemic map of malaria has gradually shrinked. However, in the past two years, the number of malaria deaths remained at a high level, and the incidence of malaria has even risen, leading to the stagnant of malaria elimination. The main reasons include lacking of the well monitoring and response system, sensitivity declining of antimalarial drugs, the spread of insecticide resistance, and the reduction of financial support. This paper introduces the progress and challenges of global malaria elimination, summarizes the current strategies and major interventions, and provides the corresponding response.

Objective: To analyze the results of samples testing of Jiangsu Provincial Malaria Diagnostic Reference Laboratory in 2017, so as to provide the evidence for improving the malaria diagnostic performance in this province., Methods: The samples of reported malaria cases in Jiangsu were collected by the provincial reference laboratory (PRL) in 2017. The microscopy and nucleic acid test were performed to confirm the infection of Plasmodium species of each case, while Plasmodium antigen tests (rapid diagnostic test, RDT) were performed as well. The detection results were analyzed among different areas and different species., Results: Totally 242 malaria cases were reported and the samples were collected by PRL in 2017. A total of 239 cases were confirmed Plasmodium infections, including 163 cases of Plasmodium falciparum infection, 21 cases of P. vivax infection, 11 cases of P. malariae infection, 43 cases of P. ovale infection, and 1 case of P. falciparum and P. ovale mixed-infection. The diagnostic coincidence rates of reported malaria case in 13 prefectures with districts were all > 80%, and the total coincidence rate was 88.8%. The species diagnostic coincidence rates of P. falciparum , P. vivax , P. malariae , and P. ovale were 98.8%, 57.1%, 63.6%, and 81.4% respectively, and the detection rates by RDT to those four species infections were 95.7%, 85.0%, 63.6% and 79.1% respectively., Conclusions: In 2017, the malaria diagnostic quality of medical technicians is generally high in Jiangsu Province. However, the diagnostic capacity is slightly different among different regions, and the ability to identify non- P. falciparum parasites remains to be improved. RDT is not ideal for the detection of non- P. falciparum infection. In the current stage of malaria elimination, the malaria diagnostic capacity of technicians in all the sectors should be strengthened and maintained.

Objective: To investigate the malaria rapid diagnostic testing (RDT) - related knowledge, attitude, practice (KAP) of primary healthcare professionals in Jiangsu Province and analyze the corresponding influencing factors., Methods: Four cities in Jiangsu Province were selected as the study sites by the typical sampling method to conduct a survey for the RDT related KAP and work satisfaction of primary healthcare professionals, and the multiple logistic regression model was used to explore the relevant influencing factors., Results: Totally 1 150 questionnaires were issued and 949 valid questionnaires were collected with the recovery rate of 82.52%. The valid questionnaires included 453 questionnaires from laboratory technicians, 466 from clinicians, and 30 from malaria prevention and control workers. Totally 83.98% of the surveyed professionals had a low mastering level of essential RDT-related knowledge. A total of 52.17% of the surveyed laboratory technicians recognized that the application of RDT technology could effectively improve the current primary-level microscopy work, and the degree of recognition of RDT technological advantage in the laboratory technicians was higher than that in the clinicians. Totally 79.25% of the surveyed laboratory technicians regarded themselves to be capable of conducting the standardized RDT operation, and 84.55% of the surveyed clinicians regarded themselves to be capable of conducting the qualified malaria clinical diagnostic practice. The key influencing factors of RDT-related KAP of primary healthcare professionals included the laboratory technicians' gender, educational level, employer's institutional level, professional title, and working years, and the employer's institutional level of clinicians., Conclusions: The primary healthcare professionals in Jiangsu Province exhibita good acceptability towards RDT technology. However, their essential knowledge on RDT remains to be improved. Therefore, it is necessary to establish a specific training and educational system for primary healthcare professionals to better guarantee the advantageous impact of RDT technology on the consolidation of the malaria elimination work achievements.

Objective: To evaluate the implementation effect of the optimization strategy for the use and management of malaria rapid diagnostic testing (RDT) technology in Jiangsu Province, so as to provide the empirical evidence and suggestions for promoting the standardized use of RDT technology., Methods: Questionnaire surveys of primary-level health professionals' RDT-related knowledge, attitude, and practice (KAP) and work satisfaction were conducted in 4 pilot cities in Jiangsu Province before and after the intervention., Results: After the implementation of the intervention, 13.9% of surveyed laboratory technicians, 21.9% of surveyed clinicians and 4.1% of surveyed staff of the centers for disease control and prevention (CDCs) had significant improvements in RDT-related knowledge. About 10.9% of the surveyed laboratory technicians and 25.6% of the surveyed CDC staff improved their attitudes toward RDT technology. About 38.4% of the surveyed laboratory technicians and 10.0% of the surveyed clinicians improved the standardized use of RDT technology. All types of primary-level health professionals had high evaluation in the satisfaction and effectiveness of the optimization strategy. However, the evaluation of the surveyed clinicians was slightly lower than that of the laboratory technicians and CDC staff., Conclusions: The optimization strategy in this project can effectively improve the knowledge, attitude and behavior of all types of primary-level health professionals and help to promote the standardized use of RDT technology.

Objective: To analyze the vector surveillance results during the stage of malaria elimination, so as to provide the evidence for assessing the local transmission risk of imported malaria and carrying out the surveillance work after malaria elimination in Jiangsu Province., Methods: From 2011 to 2017, the mosquito population was monitored and human biting rates were calculated by the half overnight human baiting trapping method and overnight lamp trapping method in 7 surveillance sites from June to October. The insecticide resistance level was tested by the force contact method recommended by WHO., Results: A total of 5 106 Anopheles mosquitoes were captured by the half over-night human baiting trapping method in the 7 sites from 2011 to 2017, and all the mosquitoes were identified as Anopheles sinensis . The annual human biting rates were 1.075, 0.786, 1.057, 0.787, 0.790, 1.797 and 1.185 mosquitoes/ (human·hour), respectively. Totally 28 186 Anopheles mosquitoes were caught by the overnight lamp trapping method, and all the mosquitoes were An. sinensis . The densities of Anopheles mosquitoes were 57.950, 50.932, 14.800, 4.405, 58.070, 72.406, and 17.145 mosquitoes/ (night·lamp), respectively. In 2012, the resistance indexes of An. sinensis to deltamethrin, DDT and malathion were at R level in Jiangsu Province., Conclusions: The major vector is An. sinensis and no An. anthropophagus is found in Jiangsu Province. An. sinensis has a high level of resistance to deltamethrin, DDT and malathion.

Objective: To understand the quality of production of blood slides and laboratory personnel's ability to microscopically examine Plasmodium at Jiangsu Province clinic inspection stations at all levels, so as to provide the evidence for the construction of malaria diagnostic laboratories and the evaluation of malaria elimination effect., Methods: After the reexamination of the negative blood slides of the examination stations of the malaria center in each county (district), the negative blood slides of the outpatient inspection station in the district and all the positive blood slides of the direct report of the network from 2011 to 2017, the staining and cleanliness of the blood smears, microscopic examination results and the coincidence rate of positive blood species were comprehensively analyzed., Results: A total of 19 109 checked blood samples were examined. The qualified rates of blood preparation, dyeing and cleanliness were 87.5%, 89.3% and 87.6%, respectively. The quality of reexamined blood slides in different years showed statistically significant difference (all P < 0.05), by using chi-square trend test, and there was a trend of increasing year by year. There was a significant difference in the quality of the reexamined blood slides among the different cities with districts ( P < 0.05). In addition, there were 1 860 blood samples from 2012 to 2017, and the total coincidence rate was 87.4% and the coincidence rate of positive blood smear examinations was 98.9%. The species coincidence rates of Plasmodium falciparum , Plasmodium vivax , Plasmodium malariae , Plasmodium ovale and mixed infection were 95.4%, 33.3%, 56.0%, 76.5% and 9.1%, respectively ( P < 0.05). The total coincidence rates of blood smear reexaminations in different years were 85.0%, 90.1%, 86.0%, 85.3%, 90.0% and 87.6% from 2012 to 2017, respectively, and the difference was not statistically significant ( P > 0.05)., Conclusions: The quality of blood slides at the primary clinic inspection stations in Jiangsu Province conforms to the standard established by the provincial malaria reference laboratory; however, the ability of Plasmodium species identification is insufficient and needs to be improved.

There were vivax malaria, falciparum malaria, and malariae malaria epidemic in Jiangsu Province, and vivax malaria was the dominant disease. Anopheles sinensis and An. anthropophagus were malaria-transmitted vectors. Since the founding of People's Republic of China, there have been two major malaria outbreaks in Jiangsu Province, when the highest annual malaria incidence reached up to 24.95%. According to prevention and control principles of "adjusting measures to local conditions, giving different guidance to different categories and highlighting key points", during the epidemic outbreak stage, the largescale malaria prevention and control measures were implemented for all the residents who were target population in Jiangsu malaria-endemic areas. During the basic eradication stage, the targeted prevention and control measures had been carried out according to the different epidemic features to gradually consolidate the achievement of prevention and control in Jiangsu Province. In the malaria elimination stage, Jiangsu Province focused on controlling of the source of infection and malaria surveillance. According to the "1-3-7 targeted elimination" work model, the management of infectious sources and investigation and disposal of foci were carried out for each epidemic focus to block the malaria transmission. By the end of 2017, there had been no indigenous malaria cases for the six consecutive years in Jiangsu Province. All the 13 cities have passed the assessment of malaria elimination of Jiangsu Province. Although Jiangsu Province has achieved the goal of malaria elimination, it is urgent and necessary to maintain and improve malaria surveillance capabilities to prevent malaria reintroduction.

Objective: To establish a systemized malaria biobank with well-rounded epidemiologic data and a computer-aid management system, thus to provide qualified sources for malaria elimination and human malaria research., Methods: The malaria biobank was based on the platform of Jiangsu Institute of Parasitic Diseases. The blood smear samples, dried blood samples, whole blood samples and parasite strains isolated from patients (from both local and imported cases) were collected since 2011 according to a standardized operational procedure. The biobank management software was applied to input of the epidemiological data and samples, and the quality of samples was monitored regularly., Results: A standard malaria biobank was established. The Information Management System was applied to input, storage and output of samples. Totally 99.42% (2 223/2 236) of the blood smear samples, 92.58% (2 070/2 236) of the dried blood samples, 94.50% (2 113/2 236) of whole blood, and 2.06% (46/2 236) of the isolated stains in 2 236 reported cases were included in the malaria biobank in Jiangsu Province from 2011 to 2017. Based on the malaria biobank, 99.42% (2 223/2 236) of the blood smears and 82.74% (1 850/2 236) of DNA (from dried blood and whole blood samples) from malaria patients in Jiangsu Province were re-checked. Moreover, the samples in the malaria biobank were used in the studies of the mechanism of parasite drug resistance, malaria molecular epidemiology, and diagnosis technology development and evaluation., Conclusions: The establishment of malaria biobank provides a guarantee for malaria elimination in Jiangsu Province and also provides a qualified resource for malaria research.

Objective: To analyze the situation of malaria elimination, identify the current main challenges and difficulties of maintaining the achievements of malaria elimination, and find out feasible solutions in Jiangsu Province., Methods: Through randomized grouping, the subject discussion and questionnaires were conducted by malaria control staffs from centers for diseases control and prevention of 13 cities of Jiangsu Province and Jiangsu Institute of Parasitic Diseases. The basic characteristics of participants and the effect of the discussion were analyzed through questionnaires., Results: Twenty-seven professional participants were randomly divided into 3 groups. Totally 27 questionnaires were released and 24 valid questionnaires were retrieved. Among the 24 participants, 79.1% (19/24) of them were with mid-level and senior professional titles, and 66.7% (16/24) of them had worked for more than 10 years. Before the group discussion, only 16.7% (4/24) of the participants were aware of the current main challenges and difficulties in Jiangsu Province. After the discussion, 87.5% (21/24) of them thought the discussion increased their understanding of the challenges and difficulties. Through the subject discussion, the expert group summarized the current challenges and difficulties as well as the solutions in malaria prevention in Jiangsu Province in 4 aspects, namely the management guarantee of prevention work, diagnosis and treatment of the cases, field epidemiological investigation and others. Finally, a framework about these discussion results was established., Conclusions: During the post-stage of malaria elimination, Jiangsu Province still faced many challenges and difficulties, such as management guarantee, case diagnosis and treatment, epidemiology survey and focus disposals, and malaria surveillance sustainability. The feasible solutions of these challenges may provide examples for other provinces and regions which are undergoing malaria elimination or in post-stage of malaria elimination.

Objective: To explore the expression characteristics of cytochrome P450 (CYP) candidate genes (CYP6M3, CYP6Y1, CYP6P5, CYP4H14, CYP4G17, CYP12F16) in Anopheles sinensis ., Methods: The samples were collected from different developmental stages (eggs, larvae, pupae and adult mosquitoes (females and males) ), and different tissues (salivary glands, malpighian tubes, midguts, ovaries, and fat bodies) of An. sinensis and the female adult mosquitoes exposed by different insecticide doses (0, 1.25, 3.75, 6.25, 12.5 μg/bottle) and time points (0, 5, 15, 30, 60 minutes), then the total RNA was extracted. Quantitative reverse transcription PCR (qPCR) assay was used to analyze the relative expressions of six CYP genes in An. sinensis at different developmental stages, tissues and different insecticide exposure doses and time., Results: The expressions of CYP6M3 and CYP6Y1 in the male adult mosquitoes were the highest, the expression of CYP6M3 gene in the males was 35.1 times higher than that in the females, the expression of CYP6Y1 in the males was 61.4 times higher than that in the females; the expression level of CYP4H14 in the larvae was the lowest, the expression of CYP4H14 in the females was 22.5 times higher than that in the fourth instar larvae. The expressions of candidate CYP genes in different tissues of An. sinensis were significantly different, the expression of CYP6M3 in the malpighian tubule was 38.9 times higher than that in the ovary, the expression of CYP6Y1 in the fatbody was 9.1 times higher than that in the ovary, the expression of CYP4G17 was 4.6 times higher than that in the ovary, and the expression of CYP12F16 was 4.4 times higher thanthat in the ovary. The exposure to different insecticide doses and time showed some induction effects in the expressions of candidate CYP genes, which affected the expressions of candidate CYP genes in An. sinensis ., Conclusions: The expression of candidate CYP genes varies significantly in different developmental stages and various tissues of An. sinensis , and exposure to deltamethrin at various doses and time points affects CYP genes expression in An. sinensis .

Objective: To analyze the working conditions and evaluate the effectiveness of blood smear examinations in fever patients after the start of national malaria elimination program (NMEP), and explore the suitable strategy for malaria diagnosis and surveillance in NMEP, so as to provide the evidence for the development and adaptation of malaria elimination strategies and measures in Jiangsu Province., Methods: The data of malaria epidemic situation reported by network, fever patients' blood test tables, and epidemiological surveys of malaria cases were collected and statistically analyzed by SPSS 16.0 in Jiangsu Province from 2010 to 2016., Results: A total of 2 367 cases of malaria cases were reported from 2010 to 2016. Among the 3 691 188 person-times of fever patients tested, there were 2 252 positives (0.06%). The number of blood tests of fever patients decreased from 742 900 in 2010 to 277 000 in 2016. At the same time, the positive rate increased from 0.04% in 2010 to 0.16% in 2016. The positive rate of blood tests of fever patients in 2016 was significantly higher than that in 2010 ( χ 2 = 161.61, P < 0.01). From 2010 to 2016 in Jiangsu province, the centers for disease control and prevention of cities and counties, that completed the blood review within 3 days, deleted 273 negative cases. In 2010, the centers for disease control and prevention of cities and counties deleted 61 negative cases, but in 2016, the centers deleted only 14 negative cases, and the negative rate of blood review in 2010 was significantly higher than that in 2016 ( χ 2 = 22.99, P < 0.01)., Conclusions: A large number of blood smear examinations have been tested in fever patients in Jiangsu Province. It not only conforms to the strong monitoring system requirements issued by World Health Organization (WHO) in malaria elimination, but also provides the evidence for no local malaria case in Jiangsu since 2012.

Objective: To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2016, so as to provide the evidence for formulating and adjusting the strategy and measures for malaria elimination., Methods: The data of malaria cases in Jiangsu Province in 2016 were collected from China's Routine Diseases Surveillance Information System (CRD-SIS) and analyzed statistically., Results: Totally, 308 malaria cases were reported in Jiangsu Province in 2016, and the cases decreased by 23.95% compared to 405 malaria cases in 2015. Except for 1 malaria case that was caused by blood transfusion, the rest patients were all imported. Among the 308 cases, 237 were infected with Plasmodium falciparum , 11 were infected with P. vivax , 8 were infected with P. malariae , 51 were infected with P. ovale , and 1 was mixed infection. Among all the cities in Jiangsu Province, the numbers of imported cases of Nantong (53 cases), Huai'an (41 cases), Taizhou (40 cases), Lianyungang (36 cases) and Yangzhou (29 cases) ranked in the top 5, and the total malaria cases in the five cities accounted for 64.61%., Conclusions: There were no local malaria cases in Jiangsu Province for 5 consecutive years up to 2016. Although the imported cases decreased slightly compared to those in 2015, we still should strengthen the surveillance of imported malaria cases as well as improve malaria diagnosis and treatment abilities for related personnel at all levels of medical institutions. Also we should focus on the Plasmodium check, focus survey and disposal to realize the aim of malaria elimination in Jiangsu Province in time.

Mosquito is a vector of many infectious diseases, and it is recognized a leading killer of human in the world. After the Belt and Road Initiative launches, more are countries involved and the international communication and cooperation are significantly growing in China. Therefore, the risk of imported infectious diseases is increasing as well, some mosquito-borne diseases which have been well controlled or seldom seen in China, will be more risky to cause locally transmission from imported cases and become the threat to people's health in China. This paper reviews the risk of major imported mosquito borne-diseases to China, and discusses the control strategy as well, so as to provide the suggestion for entry-exit inspection and control of imported mosquito-borne diseases in China.

Objective To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2015, so as to provide the evidence for malaria elimination. Methods The data of malaria cases in Jiangsu Province in 2015 were collected from China's routine diseases surveillance information system. Results Totally 405 imported cases were reported in Jiangsu Province in 2015, and the cases increased by 14.08% compared with those in 2014. All the malaria cases were imported, and 5 cases (1.23%) were from Southeast Asia and 400 (98.77%) were from 25 African countries or regions. The imported malaria cases were reported in 13 cities across Jiangsu Province, where Taizhou, Lianyungang, Nantong, Huaian and Yangzhou cities accounted for 68.64% of all the cases in the province. Jiangsu Institute of Parasitic Diseases (JIPD) reference lab checked all the cases and classified 299 falciparum malaria cases, 13 vivax malaria cases, 18 quartan malaria cases, 71 ovale malaria cases, and 4 mixed Plasmodium infection cases. Conclusions In Jiangsu Province, there are no local malaria cases for 4 consecutive years, but the imported cases are on the rise. Compared to 2014, the imported vivax cases increased significantly in 2015. It should be the key points to strengthen the surveillance of imported malaria cases, improve malaria diagnosis and treatment abilities for all levels of medical institutions, and promote the abilities of Plasmodium check, and focus survey and disposal of all Center for Disease Control (CDC) staffs across Jiangsu Province.

Objective To understand the integrated ability of parasitic disease prevention and control of professional personnel of Jiangsu Province through the contest. Methods Totally 56 players from the whole province were selected, and all the players participated in the contest. The theory knowledge and skill scores were collected and the statistical analyses were conducted. Results The average theoretical score of the participants was 88.86±15.56 and the passing rate was 91.1%. The average skill operating score was 69.16±16.01 and the passing rate was 67.9%. The average Plasmodium microscopy score was 16.54±8.09 and the passing rate was 50%. The average helminth egg microscopy score was 34.27±10.66 and the passing rate was 67.9%. There were statistical differences among the age groups and different levels of schistosomiasis endemic situation ( F = 5.10, 6.39, both P < 0.01). The theoretical knowledge including schistosomiasis, malaria, hydatid disease and others and the score rates were 91.07%, 90.94%, 85.83% and 90.93%, respectively. The hydatid disease score rate was lower ( χ 2 = 19.17, P < 0.01). The radar chart displayed that the score rates of tabletting and microscopy test in Kato-Katz film production, malaria blood film production and microscopy test were all low. Conclusion In Jiangsu Province, the participants have higher score in the theory test. However, they have lower skill test score, especially in the parasite species identification. The operational skills still need to be strengthened for center for disease control (CDC) participants.

Objective: To understand the population, density, seasonal fluctuation and nocturnal pattern of malaria vectors in Jiangsu Province, thus to provide evidences for malaria elimination in this province., Methods: Seven counties (cities, districts) were selected as the monitoring sites for malaria vectors in Jiangsu Province from 2013 to 2015. The mosquitoes were captured by human bait trapping in bed nets and mosquito-lured lamp overnight, and the seasonal fluctuation and nocturnal pattern of malaria vectors were observed., Results: A total of 11 041 Anopheles sinensis mosquitoes were captured by the mosquito-lured lamps in 7 counties of Jiangsu Province from 2013 to 2015, and no An. anthropophagous was found. Among all the 7 monitoring sites, the number of An. sinensis captured in Sihong County was the most (6 742 mosquitoes), while that in Xuyu County was the least (34 mosquitoes). During this period, the density peaks of An. sinensis were the first half of July, the first half of August and the second half of July. A total of 2 421 An. sinensis were collected in 7 monitoring sites from 2013 to 2015 by human bait trapping in bed nets overnight. Among all the 7 monitoring sites, the captured number of An. sinensis in Sihong County was the most (1 085 mosquitoes), while that in Ganyu County was the least (13 mosquitoes). The nocturnal peak of An. sinensis was from 19：00 to 20：00 and 525 An. sinensis mosquitoes were captured during this period of time, which accounted for 21.68% of the total. Hereafter, the captured number of An. sinensis reduced over time., Conclusions: The density of An. sinensis mosquitoes is still high in individual areas in Jiangsu Province, so the epidemic and vector monitoring still should be strengthened to prevent the local transmission of imported malaria.

Objective: To analyze the channels of going abroad of imported malaria patients in Jiangsu Province, so as to provide the evidence for the control of imported malaria and health intervention for high-risk population., Methods: The data of imported malaria cases in Jiangsu Province in 2015 were collected and the channels of going abroad were analyzed. The Quantum GIS 1.7.4 software was applied to map., Results: Totally 405 imported cases were reported in Jiangsu Province in 2015, and the cases increased by 14.1% compared with those in 2014. All the patients were migrant workers. The migrant workers were mostly sent abroad by construction companies [43.21% (175/405)]. The dispatching companies were mainly concentrated in Taizhou, Lianyungang, Zhenjiang and Yangzhou cities, accounting for 6.17% (25/405), 3.21% (13/405), 2.72% (11/405) and 2.22% (9/405), respectively. Totally 176 (43.46%) patients returned to China having accompanied workers, and 1.21% (9/745) of accompanied workers were found having symptoms of malaria later., Conclusions: It is difficult to monitor malaria in overseas migrant works in Jiangsu Province. The channels of going abroad of migrant workers are various and complicated, formulating the main difficulty and challenge of imported malaria control.

Objective: To evaluate the Wondfo Rapid Diagnostic Kit (Pf-LDH/Pan -pLDH) for detecting Plasmodium ovale and analyze the influence of parasitaemia, concentration and polymorphism of pLDH on the performances., Methods: A total of 100 blood samples from P. ovale patients confirmed by PCR were detected with the Wondfo Rapid Diagnostic Kit according to the manufacturers'instructions. The parasitaemia was determined by the microscopic examination. The concentration of pLDH was measured by ELISA tests. The LDH gene of P. ovale was amplified by PCR and sequenced. The influence of these three factors on the positive rate was analyzed., Results: The overall positive rate of Wondfo Rapid Diagnostic Kit was 70.0% (70/100). The positive rate was 27.3% for the samples with parasitaemia ≤ 500 parasites/μl and reached 75.0%-75.4% when parasitaemia > 500 parasites/μl. The positive rate was 6.7% for samples with a low pLDH concentration ( A values ≤ 0.100) and reached 95.1%-100% at a high pLDH concentration ( A values > 0.100). The results of sequence analysis indicated that all the samples could be divided into 2 types, P. o. curtisi and P. o. wallikeri . The gene homology of LDH between 2 types was 97%. There were 24 single nucleotide polymorphism (s) (SNPs) between 2 types, while only 3 SNPs were non-synonymous mutations. The homology of LDH amino acid sequences between 2 types was 99%; only 3 amino acids were different. The positive rates for P. o. curtisi and P. o. wallikeri were 73.1% (38/52) and 66.7% (32/48) respectively; there was no statistically significant difference ( P > 0.05)., Conclusions: The Wondfo Rapid Diagnostic Kit (Pf-LDH/Pan-pLDH) performs better than most of the similar products for the detection of P. ovale , and the positive rates are closely related to the parasitaemia and concentration of pLDH, while no related to the polymorphism of pLDH gene.

Objective: To understand the epidemic situation and influencing factors of malaria in Jiangsu Province and grasp the regularity and trend of the epidemic., Methods: According to the provincial malaria transmission situation and characteristics, Sihong County of Suqian City, which had more original cases, and Baoying County of Yangzhou City, which had more import cases, were chosen as the malaria surveillance sentinels at the state level; six counties, Yixing County of Wuxi City, Suining County of Xuzhou City, Wujin District of Changzhou City, Haian county of Nantong City, Ganyu County of Lianyugang City, and Xuyi county of Huaian City were chosen as the malaria surveillance sentinels at the provincial level. The data of basic status, blood tests of feverish patients, case studies of malaria patients, investigations and disposals of epidemic focuses and others were collected and analyzed., Results: There were 92,484 blood test samples from feverish patients in the eight surveillance sentinels in 2014, and the average rate of blood tests was 1.15%. Totally 61 cases were plasmodium positive, and the positive rata was 0.07%. All of the 61 cases were foreign imported patients diagnosed by laboratory. After the direct network reporting, the review of the blood slides and epidemiological individual surveys were finished in 3 days, and the investigations and disposals of epidemic focuses were finished in 7 days. The epidemiological individual surveys of these 61 cases showed that 37.70% (23/61) of the first-time consultancy was mainly in the county level CDC, and 50.82% (31/61) in county level medical organizations. The making definite diagnosis of malaria also focused on the county level, 45.90% (28/61) in county level CDC and 47.10% (29/61) in county level medical organizations. The definite diagnostic rate of fist-time consultancy was 90.16% (55/61). Totally 48 malaria patients had been sent to abroad by their companies, and 34 had fellow crew., Conclusions: The malaria surveillance sentinels in Jiangsu Province play a good role on the surveillance function of malaria, and effectively promote the elimination of malaria in whole province. In the future, the malaria surveillance should establish a multi-sectoral cooperation and investigation mechanism, and strengthen the screening of the high-risk population and health education.

Objective: To understand the malaria epidemic situation and characteristics in Jiangsu Province in 2014, so as to provide the evidence for formulating and adjusting appropriate strategies and measures for malaria elimination in this province., Methods: The reported malaria cases from the Internet Reporting System and the epidemiological data of malaria in Jiangsu Province were collected and analyzed., Results: A total of 355 malaria cases were reported in Jiangsu Province in 2014, which was increased by 4.11% comparing to that in 2013 (341 cases), and the malaria incidence was 0.046/10 000. All the 355 cases were imported from other countries, among which, 4 cases (1.13%) were from Southeast Asia; the other 351 cases (98.87%) were from 21 African countries. Though the cases were distributed in all the 13 prefecture-level cities in Jiangsu Province, the number of cases in 5 of them namely Huai' an, Nantong, Lianyungang, Yangzhou and Taizhou accounted for 63.38% (225/ 355). A total of 292 falciparum malaria cases, 4 tertian malaria cases, 10 quartan malaria cases, 46 ovale malaria cases and 3 mixed infection cases were confirmed after re-checked by Jiangsu Provincial Reference Lab of Malaria. The follow-up observation of the cases showed that among the 355 cases, 6 falciparum malaria cases recrudesced, and 4 ovale malaria cases and 1 tertian malaria case recurred., Conclusions: There have been no local malaria cases reported from Jiangsu Province in the last three years, indicating the object of malaria elimination has been achieved initiatively. However, there are still many imported malaria cases from other countries, with a diverse species of plasmodium. Therefore, the surveillance of the imported malaria, the training for diagnosis and treatment of malaria as well as the health education to the key population should be strengthened.

Objective: To analyze the regular surveillance and evaluate its effect during the elimination stage of malaria in Jiangsu Province from 2012 to 2014 and discuss the best way for surveillance and management, so as to provide the evidence for formulating and adjusting the strategy and methods for malaria elimination., Method: The data of network malaria reports, blood tests of feverish patients, epidemiology investigation forms of case study of malaria patients in the report system, and investigation and disposition forms of epidemic regions were collected and analyzed statistically., Results: From 2012 to 2014, there were 894 malaria cases in total. Totally 1 625 034 feverish patients had blood tests, and 892 of them showed positive and the positive rate was 0.05%. The epidemiological case study showed that all the patients were foreign infection import patients except one was foreign import cases by blood transfusion. These cases were mainly distributed in Nantong City (125 cases), Yangzhou City (123 cases), Huai'an City (112 cases), Lianyungang City (111 cases) and Taizhou City (88 cases), and the total cases of the five cities accounted for 62.53% (559/894) of total cases of entire province. These cases were mainly diagnosed in country level CDC and medical organizations, and city level medical organizations. These units accounted for 87.02% of all the diagnostic organizations (778/894)., Conclusions: From 2012 to 2014, there are no reports of local malaria infection cases in Jiangsu Province, meaning the preliminary realization of eliminating malaria. However, we still should strengthen the regular surveillance and treatment of import malaria cases.

Objective: To explore the effect of different temperatures on the different development stages of Aedes albopictus., Methods: The changes at different development stages of mosquitoes (egg, larva, pupae) and gonotrophic cycle were observed at different temperature conditions of 10, 15, 20, 25, 30, 35 °C and 40 °C. The full developmental cycles were compared during different temperatures., Results: All the stages of the mosquitoes could not develop at 10 °C. Under the different temperatures of 15, 20, 25, 30, 35 °C and 40 °C, the hatchabilities of the mosquitoes were 0, 32%, 82%, 83%, 82% and 59% respectively; the pupation rates of the mosquitoes were 38%, 53%, 84%, 88%, 72% and 42% respectively; and the emergence rates of the mosquitoes were 92%, 95%, 97%, 97%, 83% and 17% respectively. The mosquitoes could well develop at 20, 25, 30 °C and 35 °C, the development time was 37.73, 18.50, 16.92 and 13.66 days respectively., Conclusion: The development time of Aedes albopictus is shorter at the higher temperature. The optimum temperature for the mosquitoes to develop is between 25-30 °C, and higher or lower the temperatures will suppress the development of the mosquitoes.

Objective: To understand the epidemic situation and influencing factors of malaria in Jiangsu Province and grasp its epidemic regularity and trend., Methods: According to the malaria prevalence in Jiangsu Province, 6 counties (city, district) including Yixing, Suining, Wujin, Hai'an, Ganyu and Xuyi were selected as provincial surveillance sites to survey malaria epidemic conditions. The basic information, blood test results of fever patients, case investigation, information of malaria patients, monitoring data of investigation and disposition of the malaria focus were collected and analyzed., Results: In 2013, the blood tests of 66 723 fever patients were performed, the average blood smear checking rate was 1.10%, and the average positive rate was 0.08% (52 plasmodium positive individuals) in the 6 areas. For these 52 plasmodium positive individuals, the blood retests and case investigations were completed within 3 days after these cases were reported by the network system, and the investigation confirmed that they were foreign imported malaria cases. The malaria focus investigation and disposition were finished within 1 week and the data were reported by the Parasitic Diseases Information System. Four of 52 cases were recrudescence during the follow-up. Among the 52 cases, 20 people went abroad themselves and 4 were labors of private enterprises, 21 people came back without the accompanied., Conclusions: With the development of the malaria elimination program in Jiangsu Province, the eliminating malaria "targeted 1-3-7" working pattern has been comprehensively implemented. The personnel monitoring for labors who returned from overseas working will be a key in the future.

Objective: To evaluate the toxic effect of Bacillus thuringiensis var. israelensis (Bti) wettable powder against Aedes, Culex and Anopheles larvae., Methods: The biological assay was applied to test the lethal concentration of 50% (LC50) of Bti wettable powder against Aedes, Culex and Anopheles larvae., Results: The LC50(s) of Bti wettable powder against Aedes albopictus, Culex pipiens pallens and Anopheles sinensis larvae were 0.104, 0.160 microg/ml and 0.324 microg/ml, respectively; its biological potencies against them were 0.125, 0.192 IU/ml and 0.389 IU/ml, respectively. The LC50(s) of continuous contact of Bti wettable powder with An. sinensis stage III larvae for 1, 2 d and 3 d were 0.324, 0.092 microg/ml and 0.032 microg/ml, respectively, and its biological potencies were 0.389, 0.110 IU/ml and 0.038 IU/ml, respectively. The LC50(s) of the bacteria against An. sinensis stage I , II, III, IV were 0.024, 0.137, 0.324 microg/ml and 0.450 microg/ml, respectively, and the biological potencies were 0.029, 0.164, 0.389 IU/ml and 0.540 IU/ml, respectively., Conclusion: Bti wettable powder has a good toxicity to Aedes, Culex and Anopheles larvae, especially for the latter two. It is better to apply the bacteria at the early stage of mosquito larvae.

Vector control plays a crucial role in the stages of malaria control and elimination. Currently, it mainly relies on the chemical control methods for adult mosquitoes in malaria endemic areas, however, it is undergoing the serious threat by insecticide resistance. In recent years, the transgenic technologies of malaria vectors have made a great progress in the laboratory. This paper reviews the challenges of the traditional methods and the rapid developed genetic modified technology in the application of vector control.

Objective: To understand the deltamethrin resistance level, GTS and P450 metabolic detoxification enzyme activities, and mutations in the para-type sodium channel gene that confers knockdown resistance (kdr) in Anopheles sinensis mosquitoes from malaria endemic areas along the Huaihe River in Anhui Province, China., Methods: An. sinensis adult mosquitoes were collected in Lilou, Mohekou and Tuohu townships of Bengbu City, Anhui Province from August to September, 2011. The insecticide resistance bioassays were performed on adult mosquitoes by using the standard WHO susceptibility test with diagnostic concentrations of deltamethrin 0.05%, and the mosquito resistance status was classified based on the WHO resistance classification criteria. The metabolic detoxification enzyme activities were measured in randomly selected mosquitoes, and the IIS6 region of the para-type sodium channel gene was amplified by PCR and sequenced to detect mutations at the codon 1014., Results: The knockdown rates within 60 min exposure to deltamethrin test paper were 4.1%, 7.0% and 8.2%, and the mortality rates were 8.2%, 12.0% and 12.8% for mosquitoes collected from Lilou, Mohekou and Tuohu townships, respectively. These three populations were classified as highly resistant populations based on the WHO resistance classification criteria. The GST and P450 enzyme activities of the three populations were significantly higher than those of the susceptible laboratory population (P < 0.001). L1014C and L1014F mutations were detected, and the wild type homozygote kdr genotype was not found. These three populations exhibited a small but insignificant difference in kdr allele frequencies. No mutation was found in the laboratory susceptible mosquitos., Conclusion: The An. sinensis mosquito populations from the regions along the Huaihe River in Anhui Province are strongly resistant to pyrethroid insecticides, and exhibit significantly higher metabolic detoxification enzyme activities than the laboratory susceptible population. The high frequency of kdr mutation is identified in the mosquito populations from the region along the Huaihe River in Anhui Province, China.

Objective: To establish a Real-time Fluorescence Quantitative PCR to detect the kdr gene mutation in Anopheles sinensis., Methods: One pair of primers and three TaqMan-MGB probes were designed based on kdr gene and its L1014 locus mutations of A. sinensis. After optimization, the Real-time Fluorescence Quantitative PCR was verified by using 6 types of A. sinensis samples with different kdr gene types. Additionally, 50 laboratory samples and 113 field samples were tested by this method., Results: The established Real-time Fluorescence Quantitative PCR could identify 6 different kdr gene types in A. sinensis. The mutation could be detected by single-tube Fluorescence Quantitative PCR, and the detail mutation type could be further identified by double-tube Fluorescence Quantitative PCR. By using this method, 50 laboratory samples were confirmed as wild type homozygotes. Among 113 field samples, 12 were wild type homozygotes, others were L1014F or L1014C mutations, and the total mutation frequency was 87.61%., Conclusion: The new established TaqMan-MGB Real-time Fluorescence Quantitative PCR can be used to detect the kdr gene L1014 mutations of A. sinensis.

Objective: To observe the developmental threshold temperature and the effective accumulated temperature of Plasmodium vivax sporozoites in Anopheles sinensis in Jiangsu Province, and to analyze the impact of temperature on the development of Plasmodium vivax., Methods: The Anopheles sinensis mosquitoes were maintained under different temperatures of 16 +/- 0.5, 19 +/- 0.5, 22 +/- 0.5, 25 +/- 0.5, 28 +/- 0.5 degrees C and 31 +/- 0.5 degrees C in incubators after membrane feeding with Plasmodium vivax infected blood at laboratory. The salivary glands of Anopheles sinensis were dissected to confirm the development of sporozoite under different temperatures, and the developmental threshold temperature and the effective accumulated temperature of Plasmodium vivax sporozoites in Anopheles sinensis were calculated. The theoretical number of generations of Plasmodium vivax in Anopheles sinensis per year was calculated based on the average monthly temperatures of 13 municipalities in Jiangsu Province., Results: The average developmental threshold temperature of Plasmodium vivax in Anopheles sinensis in Jiangsu Province was 15.31 degrees C, the average effective accumulated temperature was 109.81 day-degrees, and the optimal temperature for the proliferation of Plasmodium vivax was 25-28 degrees C. The theoretical generation number of Plasmodium vivax in sinensis in south Jiangsu was 1-3 generations being more than that in the north of the province., Conclusions: Temperature is one of the important influencing factors for the development of Plasmodium. The chance for Anopheles sinensis to transmit malaria increases under the temperature of 25-28 degrees C. However, other factors should be considered in the establishment of early warning system of malaria.

Objective: To establish a fluorescent quantitative PCR (FQ-PCR) method for quantitative detection and species identification of Plasmodium sporozoites in Anopheles mosquitoes., Methods: One pair of human Plasmodium genus-specific primers based on 18S rRNA genes were used and the reaction system and reaction condition of FQ-PCR were optimized by using the mixture of Plasmodium 18S rRNA gene recombinant plasmids and Anopheles DNA as a template. The specificity was verified by using four Plasmodium spp. 18S rRNA gene plasmid DNA as well as mosquito DNA and the Plasmodium species was identified according to the value of melting temperature (Tm). The standard curve was made by using P. vivax 18S rRNA gene recombinant plasmids which were serially diluted by negative Anopheles DNA as a template. The sensitivity was analysed by using plasmid DNA and laboratory infected sporozoite positive mosquito DNA, respectively. The different parts and different amounts of Anopheles DNA were added into the reaction system to investigate the influence of Anopheles DNA on the assessment., Results: There was no specific amplification for mosquito DNA and human blood DNA. There was specific amplification for Plasmodium 18S RNA gene recombinant plasmids and the Tm(s) of P. malariae, P. falciparum, P. ovale and P. vivax were 71.0, 72.7, 73.9 degrees C and 75.9 degrees C, respectively, which were easy to be identified. The standard curve indicated a good linear relationship between the cycle threshold (Ct) and template concentration (r = -0.99). The sensitivity was 50 copies of plasmid DNA or one sporozoite positive mosquito DNA diluted by 32 times of mosquito DNA. Anopheles DNA could inhibite the FQ-PCR reaction. The Ct value of amplification showed a good reproducibility both within the same experiment and among different experiments., Conclusion: The novel SYBR Green I based FQ-PCR method developed in this study shows a high sensitivity and specificity and it can be used for quantitative detection and species identification of sporozoites in mosquitoes.

Objective: To establish a novel molecular identification method for discrimination of members within Anopheles hyrcanus complex., Methods: The sequences of the ribosomal DNA second internal transcribed spacer (rDNA ITS2) region of An. hyrcanus complex, including An. anthropophagus, An. lesteri, An. sinesis and An. yatsushiroensisi were analyzed by using molecular biology software Vector NTI 9.0, and a specificity restriction enzyme was selected based on the restriction fragment length polymorphism. Thus the single enzyme digestion PCR-RFLP method was established for genetic identification of An. hyrcanus complex, and 452 anopheline mosquitoes captured in the field were tested, comparing with the results of the previously established double enzyme digestion PCR-RFLP method and traditional morphological classification., Results: The molecular software analysis revealed that the restriction enzyme Dde I could digest rDNA ITS2 region of An. hyrcanus complex into different fragments, thus it could be used for single enzyme PCR-RFLP for An. hyrcanus complex identification, and the result was further confirmed by laboratory experiment. Furthermore, a total of 452 anopheline mosquitoes captured from 4 malaria endemic areas were tested by this single enzyme digestion PCR-RFLP method, and 20 of them were identified as An. anthropophagus, 6 as An. lesteri, 391 as An. sinesis, and 35 as An. yatsushiroensisi. The results were 100% accordant to the double enzyme digestion PCR-RFLP method, and 93.4% accordant to the traditional morphological classification., Conclusions: The newly established single enzyme digestion PCR-RFLP method can be used for An. hyrcanus complex identification, and is more simple and reliable than the traditional morphological classification, and it is a suitable tool for field entomology surveillance.

Objective: To investigate Anopheline species in Chayu County of Linzhi Prefecture, Tibet., Methods: Four natural villages in Chayu County were selected in this study in 2010. The methods of overnight/semi-overnight trapping indoor and outdoor human-bait, and overnight trapping with light traps were used, and all the Anopheline mosquitoes were confirmed by morphological characteristics., Results: Totally 2 991 Anopheline mosquitoes were captured, with 76.36% (2 284/2 291) of An. peditaeniatus, 22.30% (667/2 291) of An. maculates group. The average densities of An. peditaeniatus by semi-overnight trapping were 56.2/per person indoor and 4/per person outdoor respectively, and the average densities of An. maculatus group by semi-overnight trapping were 17.8/per person indoor and 17.9/per person outdoor respectively. The man-biting rates of An. peditaeniatus by overnight trapping were 28.1/per person indoor and 2/per person outdoor respectively, and were both 8.9/per person to An. maculatus group., Conclusion: An. peditaeniatus and An. maculatus group are the possible transmission vectors of malaria in Chayu County.

Objective: To explore the application value of multiplex PCR in the diagnosis of malaria in field., Methods: The plasmodium genus-specific primer, Plasmodium vivax, P. falciparum species-specific primers were synthesis based on the specific target segments of small subunit of 18 S rRNA ribosomal. The multiplex PCR system was optimized, and a PCR diagnostic method of malaria was established based on the genomic specific DNA fragment of P. vivax, and P. falciparum was amplified in the same PCR reaction system. The sensitivity, specificity, and the value of field application of the multiplex PCR were investigated., Results: The sizes of amplification products of multiplex PCR amplifying genomic DNA of P. vivax and P. falciparum were 833 bp and 1 451 bp, respectively, and the amplification did not take place with the samples DNA of P. berghei, P. cynomolgus and healthy human blood. The sensitivities of multiplex PCR to detect P. vivax and P.falciparum were 1.1 x 10(-6) and 5.6 x 10(-7) parasitemia, respectively. Compared with the microscopic examination, the positive rate of multiplex PCR to detect 119 cases of field samples was 54%, missed diagnosis rate was 0.8%, and the misdiagnosis rate was naught, while the positive rate of the microscopic examination was 53%, its misdiagnosis rate and missed diagnosis rate were 1.7% and 3.4%, respectively. The compliance rate between the multiplex PCR and microscopic examination was 94%., Conclusion: The multiplex PCR for detecting malaria is simple, rapid, specific, sensitive, etc., which is suitable for the differential diagnosis of suspected cases, and molecular epidemiology investigation.

Objective: To observe the development and breeding of Anopheles anthropophagus in Jiangsu Province under different temperatures, so as to provide theoretical evidence for malaria epidemiology research and vector control., Methods: Anopheles anthropophagus was maintained under different temperatures of 16, 19, 22, 25, 28 and 31 degrees C in cultivator, and the development of egg stage, larva, pupal stage and reproductive and vegetative cycle were observed. Then the time of entire developmental cycle of Anopheles anthropophagus at different temperatures was calculated., Results: Anopheles anthropophagus could not complete its entire life cycle under the temperature of 16 degrees C, and it took 30.68, 23.31, 15.46, 13.60 and 12.41 d to finish its life cycle at the temperatures of 19, 22, 25, 28 and 31 degrees C, respectively., Conclusions: In the temperature range of 16 to 31 degrees C, the developmental cycle of Anopheles anthropophagus in Jiangsu Province will be shortened when the temperature goes up, but whether the result can be extrapolated to other regions still needs to be verified.

Objective: To analyze the prevalent characteristics of malaria in Jiangsu Province in 2009, so as to provide the reference for making effective measures of malaria control., Methods: The reported cases and epidemiological data of malaria in Jiangsu Province were analyzed statistically and described., Results: A total of 389 malaria cases were reported with an incidence rate of 0.05/10 000 in 2009, 41.77% lower than that in 2008. There were reported malaria cases in all thirteen cities, and 44.73% of all the cases were from Xuzhou, Suqian, and Huai'an. There were 192 local malaria infectors with Plasmodium vivax (P.v) in Jiangsu Province. Majority of the local malaria infectors (73.44%) distributed in Xuzhou, Suqian, and Huai'an City. There were 96 imported infectors of P.v, mainly spreading over the south part of Jiangsu Province with a high percentage of migrant workers. There were 101 imported infectors of Plasmodium falciparum (P. f), 71.19% higher than that in 2008, mainly spreading in the central and north part of Jiangsu Province with a high percentage of workers who worked abroad., Conclusions: The results show a decreased trend of malaria incidence in Jiangsu Province, however, the malaria situation in north part to the Huai River is still instable, and there are increasing imported cases of P. f as well. It is necessary to formulate the policy and measures to strengthen malaria control in target areas, especially the monitoring and management of workers who have once worked abroad.

Objective: To understand the species, density and seasonal variation of malaria vectors in Jiangsu Province, so as to provide scientific evidence for malaria control., Methods: From 2005 to 2009, 5 towns in Jiangsu Province were selected as surveillance sites, and the species of malaria vectors and their density and seasonal variation were studied by the outdoor and indoor trapping methods. The data of malaria cases were analyzed by the circular distribution method., Results: Only Anopheles sinensis was captured in the 5 surveillance sites from 2005 to 2009, and its density peak was mainly appeared in the first half of July. The peak incidence of malaria was on 16th August, the distribution of cases was accordant with the seasonal variation of vectors., Conclusion: The surveillance and control of vectors should still be strengthened in the malaria control, so as to prevent the epidemic from rebounding as the increase of the density of Anopheles sinensis.

Objective: To understand the sensitivity of Anopheles sinensis to deltamethrin, DDT and malathion in Jiangsu province., Methods: The adult mosquitoes were captured from the fields of Sihong, Yangzhong and Yixing counties (cities) and cultured, and by using the method recommended by WHO, their first filial generations knocked down at 10, 15, 20, 30, 40, 50, and 60 min after exposure with insecticides aforementioned were counted, and the mortality of mosquitoes tested after 24 h was recorded., Results: The knock down rates of mosquitoes, which were the first generation (F0) of Anopheles sinensis captured from the field of Sihong, Yangzhong, Yixing counties (cities) to 0.05% deltamethrin were 28.57%, 57.14%, 52.38%, respectively 60 min after the exposure; and the mortality rates 24 h-post-exposure were 35.71%, 57.14%, 61.90%, respectively. The resistance degree to deltamethrin was assessed as "R" level. The knock down rates of mosquitoes 60 min after the exposure to 4% DDT were 9.52%, 2.38%, 4.76%, respectively, and the mortality rates 24 h-post-exposure were 47.62%, 50.00%, 40.48%, respectively. The resistance degree to DDT was assessed as "R" level. The knock down rates of mosquitoes 60 min after the exposure to 5% malathion were 11.90%, 28.57%, 28.13%, respectively, and the mortality rates 24 h after the exposure were 80.95%, 85.71%, 93.75%, respectively. The resistance degree to malathion was assessed as level "M". The knock down rates of Anopheles sinensis captured in day 1, day 7, day 15 and the F1 from Yixing 60 min after the exposure to 0.05% deltamethrin were 54.76%, 76.19%, 92.86%, and 52.38%, respectively, and the mortality rates post-24 h were 54.76%, 76.19%, 95.23% and 61.90%, respectively. The difference of mortality post-24 h between the mosquitoes of 1 day post-captured and F1 was not statistically significant (P > 0.05). The knock down rates 60 min after the exposure to 0.05% deltamethrin to the female and male F1 of Anopheles sinensis from Sihong were 28.57% and 40.48%, and the mortality rates post-24 h were 35.71%, 42.86% respectively, and the difference was not statistically significant (P > 0.05)., Conclusions: Anopheles sinensis in the field of Jiangsu Province has developed severe resistance to deltamethrin and DDT, initial resistance to malathion. In order to prevent the development of resistance to the insecticides, the integrated management measures should be adopted in the future.