35 results
Search Results
2. Autonomous flow control device modelling and completion optimisation.
- Author
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Eltaher, Eltazy, Muradov, Khafiz, Davies, David, and Grassick, Peter
- Subjects
- *
PETROLEUM reservoirs , *DEUTERIUM oxide , *RESERVOIRS , *HYDRAULICS , *HEAVY oil , *ENGINEERING models , *OIL fields - Abstract
Abstract Wells equipped with flow control devices are a proven method of improving sweep efficiency by controlling the influx of fluids into the well by the addition of a flow restriction at each completion joint. Autonomous Flow Control Device (AFCD) technology has recently achieved the milestone of being approved for full field, commercial application to reduce (1) gas influx in light oil reservoirs and (2) water production in heavy oil reservoirs. Autonomous Flow Control Completions (AFCC) are frequently designed using steady-state well production simulators. The more realistic approach of using a dynamic, reservoir simulator often reduces the exaggerated, single well, production improvement by allowing the reservoir's flow dynamics to be understood in the context of the total field recovery and reservoir's uncertainty. The modelling of AFCCs, as currently implemented in reservoir simulators, is in an early stage of development and requires further improvement. AFCC modelling options in a dynamic, reservoir simulator are discussed in this paper. New workflows for AFCC single-phase performance parametrization with a more reliable, physically realistic model of their performance are presented. It is shown how reservoir simulation of an Advanced Well Completion (AWC) containing ICDs and AICDs can be used to evaluate the control of water production in a heavy oil field. The performance of multiple AWC designs is compared for a range of reservoir simulation models that illustrate various production challenges. New comparison methods are introduced that provide a fundamental understanding of their differences. An oil/water flow modelling workflow for reservoir and well engineering studies for the selection of the optimal (A)FCD completion is provided. Our finding aid engineers to reliably model, and evaluate the "added value", of AFCC technology on a case-by-case basis. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
3. Evaluating the performance of advanced wells in heavy oil reservoirs under uncertainty in permeability parameters
- Author
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Ali Moradi, Nastaran A. Samani, Amaranath S. Kumara, and Britt M.E. Moldestad
- Subjects
Uncertainty assessment ,Advanced wells ,Water cut ,Heavy oil ,ICD ,AICD ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
Advanced wells play a crucial role in maximizing the efficiency of oil production. To achieve a successful design for advanced wells, several parameters must be considered and evaluated. Uncertainty in these parameters can have a significant impact on the performance assessment of the well in its lifetime. Absolute permeability, relative permeability, and permeability anisotropy are the parameters that determine reservoir permeability and are among the most important design parameters to be considered. This paper aims to assess the performance of advanced wells completed with passive and reactive downhole Flow Control Devices (FCDs) under uncertainty in the reservoir permeability parameters. The assessment is conducted through a case study on a synthetic heavy oil reservoir with a strong water drive. The EclipseSM reservoir simulator is used as a simulation tool and the Latin Hypercube Sampling (LHS) approach is applied as a sampling tool for uncertainty analysis in this study. According to the obtained results, under the presence of uncertainty, advanced wells with Autonomous Inflow Control Device (AICD) and Autonomous Inflow Control Valve (AICV) completions are able to mitigate the risk of water production by 53.20% and 71.12% respectively compared to conventional wells. However, Inflow Control Device (ICD) completion can only reduce the risk by 0.87%.
- Published
- 2022
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4. Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics.
- Author
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Wang, Peipei, Hu, Junmei, Duan, Jiaqi, Min, Shitong, Chen, Congliang, Zhu, Yue, Pan, Yan, Wang, Yitian, Wei, Dapeng, and Wang, Xia
- Subjects
COMMON bean ,MONOCLONAL antibodies ,CELL aggregation ,T cells ,CELL death ,POISONS - Abstract
Background: Leukocyte phytohemagglutinin (PHA-L), derived from the L4 tetramer of PHA, has been frequently employed as a mitogen to induce T lymphocyte proliferation in vitro. The biological application of PHA-L in cancer diagnosis and treatment has gained traction in recent years. However, it has been noted that PHA-L obtained using traditional procedures has a massive amount of impurities or toxic components, which interfere with the activity of PHA-L. Preparation of a monoclonal antibody against active PHA-L is a significant tool for studying PHA-L's function and therapeutic potential. Results: We successfully prepared monoclonal antibodies against the active components of PHA-L based on the whole PHA-L protein as an antigen, and found that monoclonal antibody 3C1C6G11 can be employed in western blot, immunofluorescence, and immunohistochemistry detection. Importantly, preliminary result shows that the mAb 3C1C6G11 may prevent PHA-L-induced cell aggregation and AICD (activation-induced cell death). Conclusions: The monoclonal antibody mAb 3C1C6G11 prepared in this study can be used as an effective tool for detecting PHA-L active components, investigating PHA-L's function and antineoplastic application. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
5. Assessment of the performance of six indices in predicating the aromaticity of planar porphyrinoids
- Author
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Ding, Wenjing, Zhang, Zhan, Chen, Xi, and Zhan, Chang-guo
- Published
- 2023
- Full Text
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6. Novel affine‐invariant curve descriptor for curve matching and occluded object recognition.
- Author
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Fu, Huijing, Tian, Zheng, Ran, Maohua, and Fan, Ming
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OBJECT recognition (Computer vision) ,CURVES ,MATCHING theory ,IMAGE registration ,IMAGE segmentation - Abstract
The authors present a new approach for affine distorted planar curve matching and exploit it for occluded object recognition. There are two main contributions in the study: First, a novel affine‐invariant curve descriptor (AICD) based on a new‐defined affine‐invariant signature and its unsigned sum is proposed to represent the local shape of a curve with high distinctiveness. Second, a part‐to‐part curve matching algorithm is developed by combining AICD with a curve segmentation strategy based on inflexion points, which can be applied to object recognition under affine distortions and partial occlusions. Experimental results demonstrate that the proposed method exhibits effectiveness in occluded object recognition better than the state‐of‐the‐art partial curve matching methods. [ABSTRACT FROM AUTHOR]
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- 2013
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7. Impairment of gamma/delta T lymphocytes in elderly: implications for immunosenescence
- Author
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Colonna-Romano, Giuseppina, Aquino, Alessandra, Bulati, Matteo, Lio, Domenico, Candore, Giuseppina, Oddo, Gioacchino, Scialabba, Giuseppe, Vitello, Salvatore, and Caruso, Calogero
- Subjects
- *
LYMPHOCYTES , *CELLS , *LEUCOCYTES , *T cells - Abstract
Gamma/delta T lymphocytes cells recognize the antigen in a non-classical way and are considered the third branch of the immune system devoted to defend the integrity of the body. Ageing is characterized by an impairment of the main way of protection (the adaptive branch) but, successfully aged people show compensatory mechanisms of defense such as proneness to inflammation. Moreover, very old subjects show an increased number of NK cells. We have previously demonstrated that γδT lymphocytes are reduced in elderly. In the present paper we have studied some characteristics of these cells to evaluate the possibility that these cells might balance the decreased action of the adaptive branch in successfully aged people. Cytofluorimetric analysis of cells collected from young, old and centenarian subjects has been used to evaluate the ability of these cells to expand in vitro. Here we demonstrate that γδT cells are impaired in the ability to proliferate to different stimuli such as isopentenyl pyrophoshate, that select γδT lymphocytes bearing δ2 chain, other than to phytohemagglutinin and anti-CD3 that are polyclonal activators. Moreover, we demonstrate that γδT cells in aged and centenarians show an enhanced sensitivity to undergo apoptosis induced both by α-Fas and TNF-α. All together these data suggest that γδT lymphocytes are impaired in elderly and suggest that the reduced ability to proliferate and the reduced number of circulating γδT lymphocytes is due to the proneness to apoptosis. Finally on the basis of these data, we conclude that γδT lymphocytes, do not participate in the remodeling of the immune system due to the reduction of classical T cell response and replacement by NK cells in elderly. [Copyright &y& Elsevier]
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- 2004
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8. A delta-secretase-truncated APP fragment activates CEBPB, mediating Alzheimer's disease pathologies.
- Author
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Yao, Yinan, Kang, Seong Su, Xia, Yiyuan, Wang, Zhi-Hao, Liu, Xia, Muller, Thorsten, Sun, Yi E, and Ye, Keqiang
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ALZHEIMER'S disease ,PATHOLOGY ,PROTEIN precursors ,TAU proteins ,CEREBRAL amyloid angiopathy ,LABORATORY mice ,MANTLE cell lymphoma ,PROTEIN metabolism ,RESEARCH ,ANIMAL experimentation ,RESEARCH methodology ,PROTEOLYTIC enzymes ,MEDICAL cooperation ,EVALUATION research ,COMPARATIVE studies ,GENES ,MICE - Abstract
Amyloid-β precursor protein (APP) is sequentially cleaved by secretases and generates amyloid-β, the major components in senile plaques in Alzheimer's disease. APP is upregulated in human Alzheimer's disease brains. However, the molecular mechanism of how APP contributes to Alzheimer's disease pathogenesis remains incompletely understood. Here we show that truncated APP C586-695 fragment generated by δ-secretase directly binds to CCAAT/enhancer-binding protein beta (CEBPB), an inflammatory transcription factor, and enhances its transcriptional activity, escalating Alzheimer's disease-related gene expression and pathogenesis. The APP C586-695 fragment, but not full-length APP, strongly associates with CEBPB and elicits its nuclear translocation and augments the transcriptional activities on APP itself, MAPT (microtubule-associated protein tau), δ-secretase and inflammatory cytokine mRNA expression, finally triggering Alzheimer's disease pathology and cognitive disorder in a viral overexpression mouse model. Blockade of δ-secretase cleavage of APP by mutating the cleavage sites reduces its stimulatory effect on CEBPB, alleviating amyloid pathology and cognitive dysfunctions. Clearance of APP C586-695 from 5xFAD mice by antibody administration mitigates Alzheimer's disease pathologies and restores cognitive functions. Thus, in addition to the sequestration of amyloid-β, APP implicates in Alzheimer's disease pathology by activating CEBPB upon δ-secretase cleavage. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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9. Siva-1 negatively regulates NF-κB activity: effect on T-cell receptor-mediated activation-induced cell death (AICD)
- Author
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Gudi, R, Barkinge, J, Hawkins, S, Chu, F, Manicassamy, S, Sun, Z, Duke-Cohan, J S, and Prasad, K V S
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- 2006
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10. Therapeutic effect of kaempferol on atopic dermatitis by attenuation of T cell activity via interaction with multidrug resistance-associated protein 1.
- Author
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Lee, Hyun‐Su, Jeong, Gil‐Saeng, Lee, Hyun-Su, and Jeong, Gil-Saeng
- Subjects
MULTIDRUG resistance-associated proteins ,ATOPIC dermatitis ,MULTIDRUG resistance ,TREATMENT effectiveness ,T cells ,CYTOKINES ,FLAVONOLS ,DNA-binding proteins ,CARRIER proteins ,MICE ,ANIMALS - Abstract
Background and Purpose: Kaempferol is a natural flavonoid widely investigated in various fields due to its antioxidant, anti-cancer, and anti-inflammatory activities, but few studies have shown its inhibitory effect on T cell activation. This study examined the therapeutic potential of kaempferol in atopic dermatitis by modulating T cell activation.Experimental Approach: Effects of kaempferol on T cell activation and the underlying mechanisms were investigated in Jurkat cells and mouse CD4+ T cells. A model of atopic dermatitis in mice was used to determine its therapeutic potential on T cell-mediated conditions in vivo. Western blots, RT-PCR, pulldown assays and ELISA were used, along with histological analysis of skin.Key Results: Pretreatment with kaempferol reduced CD69 expression and production of inflammatory cytokines including IL-2 from activated Jurkat cells and murine CD4+ T cells without cytotoxicity. Pulldown assays revealed that kaempferol physically binds to MRP-1 in T cells, inhibiting the action of MRP-1. In activated T cells, kaempferol suppressed JNK phosphorylation and the TAK1-IKKα mediated NF-κB pathway. Oral administration of kaempferol to mice showed improved manifestation of atopic dermatitis, a T cell-mediated condition. Western blot results showed that, as in the in vitro studies, decreased phosphorylation of JNK was associated with down-regulated MRP-1 activity in vivo, in the kaempferol-treated mice in the atopic dermatitis model.Conclusion and Implications: Kaempferol regulates T cell activation by inhibiting MRP-1 activity in activated T cells, thus showing protective effects against T cell mediated disease in vivo. [ABSTRACT FROM AUTHOR]- Published
- 2021
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11. Lysine acetyltransferase Tip60 acetylates the APP adaptor Fe65 to increase its transcriptional activity.
- Author
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Probst, Sabine, Riese, Florian, Kägi, Larissa, Krüger, Maik, Russi, Natalie, Nitsch, Roger M., and Konietzko, Uwe
- Subjects
ACETYLTRANSFERASES ,AMYLOID beta-protein precursor ,LYSINE ,SIRTUINS ,ADAPTOR proteins ,GLUTAMINE ,ACETYLATION - Abstract
Proteolytic processing of the amyloid precursor protein (APP) releases the APP intracellular domain (AICD) from the membrane. Bound to the APP adaptor protein Fe65 and the lysine acetyltransferase (KAT) Tip60, AICD translocates to the nucleus. Here, the complex forms spherical condensates at sites of endogenous target genes, termed AFT spots (AICD-Fe65-Tip60). We show that loss of Tip60 KAT activity prevents autoacetylation, reduces binding of Fe65 and abolishes Fe65-mediated stabilization of Tip60. Autoacetylation is a prerequisite for AFT spot formation, with KAT-deficient Tip60 retained together with Fe65 in speckles. We identify lysine residues 204 and 701 of Fe65 as acetylation targets of Tip60. We do not detect acetylation of AICD. Mutation of Fe65 K204 and K701 to glutamine, mimicking acetylation-induced charge neutralization, increases the transcriptional activity of Fe65 whereas Tip60 inhibition reduces it. The lysine deacetylase (KDAC) class III Sirt1 deacetylates Fe65 and pharmacological modulation of Sirt1 activity regulates Fe65 transcriptional activity. A second acetylation/deacetylation cycle, conducted by CBP and class I/II KDACs at different lysine residues, regulates stability of Fe65. This is the first report describing a role for acetylation in the regulation of Fe65 transcriptional activity, with Tip60 being the only KAT tested that supports AFT spot formation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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12. CD5 blockade enhances ex vivo CD8+ T cell activation and tumour cell cytotoxicity.
- Author
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Alotaibi, Faizah, Rytelewski, Mateusz, Figueredo, Rene, Zareardalan, Ronak, Zhang, Meng, Ferguson, Peter J, Maleki Vareki, Saman, Najajreh, Yousef, El‐Hajjar, Mikal, Zheng, Xiufen, Min, Wei‐ping, and Koropatnick, James
- Subjects
T cells ,TUMORS ,B cells ,TREATMENT effectiveness ,CELLULAR immunity - Abstract
CD5 is expressed on T cells and a subset of B cells (B1a). It can attenuate TCR signalling and impair CTL activation and is a therapeutic targetable tumour antigen expressed on leukemic T and B cells. However, the potential therapeutic effect of functionally blocking CD5 to increase T cell anti‐tumour activity against tumours (including solid tumours) has not been explored. CD5 knockout mice show increased anti‐tumour immunity: reducing CD5 on CTLs may be therapeutically beneficial to enhance the anti‐tumour response. Here, we show that ex vivo administration of a function‐blocking anti‐CD5 MAb to primary mouse CTLs of both tumour‐naïve mice and mice bearing murine 4T1 breast tumour homografts enhanced their capacity to respond to activation by treatment with anti‐CD3/anti‐CD28 MAbs or 4T1 tumour cell lysates. Furthermore, it enhanced TCR signalling (ERK activation) and increased markers of T cell activation, including proliferation, CD69 levels, IFN‐γ production, apoptosis and Fas receptor and Fas ligand levels. Finally, CD5 function‐blocking MAb treatment enhanced the capacity of CD8+ T cells to kill 4T1‐mouse tumour cells in an ex vivo assay. These data support the potential of blockade of CD5 function to enhance T cell‐mediated anti‐tumour immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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13. Macroautophagy inhibition maintains fragmented mitochondria to foster T cell receptor-dependent apoptosis.
- Author
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Corrado, Mauro, Mariotti, Francesca R, Trapani, Laura, Taraborrelli, Lucia, Nazio, Francesca, Cianfanelli, Valentina, Soriano, Maria Eugenia, Schrepfer, Emilie, Cecconi, Francesco, Scorrano, Luca, and Campello, Silvia
- Subjects
AUTOPHAGY ,MITOCHONDRIA ,T cells ,APOPTOSIS ,MITOCHONDRIAL membranes ,CELL death - Abstract
Mitochondrial dynamics and functionality are linked to the autophagic degradative pathway under several stress conditions. However, the interplay between mitochondria and autophagy upon cell death signalling remains unclear. The T-cell receptor pathway signals the so-called activation-induced cell death ( AICD) essential for immune tolerance regulation. Here, we show that this apoptotic pathway requires the inhibition of macroautophagy. Protein kinase-A activation downstream of T-cell receptor signalling inhibits macroautophagy upon AICD induction. This leads to the accumulation of damaged mitochondria, which are fragmented, display remodelled cristae and release cytochrome c, thereby driving apoptosis. Autophagy-forced reactivation that clears the Parkin-decorated mitochondria is as effective in inhibiting apoptosis as genetic interference with cristae remodelling and cytochrome c release. Thus, upon AICD induction regulation of macroautophagy, rather than selective mitophagy, ensures apoptotic progression. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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14. New Insights into Epigenetic and Pharmacological Regulation of Amyloid-Degrading Enzymes.
- Author
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Nalivaeva, Natalia, Belyaev, Nikolai, and Turner, Anthony
- Subjects
AMYLOID beta-protein ,ALZHEIMER'S disease treatment ,DOWNREGULATION ,EPIGENETICS ,NEPRILYSIN ,INSULINASE ,RETINOID X receptors ,THERAPEUTICS - Abstract
Currently, deficit of amyloid β-peptide (Aβ) clearance from the brain is considered as one of the possible causes of amyloid accumulation and neuronal death in the sporadic form of Alzheimer's disease (AD). Aβ clearance can involve either specific proteases present in the brain or Aβ-binding/transport proteins. Among amyloid-degrading enzymes the most intensively studied are neprilysin (NEP) and insulin-degrading enzyme (IDE). Since ageing and development of brain pathologies is often accompanied by a deficit in the levels of expression and activity of these enzymes in the brain, there is an urgent need to understand the mechanisms involved in their regulation. We have recently reported that NEP and also an Aβ-transport protein, transthyretin are epigenetically co-regulated by the APP intracellular domain (AICD) and this regulation depends on the cell type and APP isoform expression in a process that can be regulated by the tyrosine kinase inhibitor, Gleevec. We have now extended our work and shown that, unlike NEP, another amyloid-degrading enzyme, IDE, is not related to over-expression of APP in neuroblastoma SH-SY5Y cells but is up-regulated by APP and APP isoforms independently of AICD but correlating with reduced HDAC1 binding to its promoter. Studying the effect of the nuclear retinoid X receptor agonist, bexarotene, on NEP and IDE expression, we have found that both enzymes can be up-regulated by this compound but this mechanism is not APP-isoform specific and does not involve AICD but, on the contrary, affects HDAC1 occupancy on the NEP gene promoter. These new insights into the mechanisms of NEP and IDE regulation suggest possible pharmacological targets in developing AD therapies. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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15. Hypoxia Affects Neprilysin Expression Through Caspase Activation and an APP Intracellular Domain-dependent Mechanism.
- Author
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Turner, Anthony J., Kerridge, Caroline, Nalivaeva, Natalia N., and Kozlova, Daria I.
- Subjects
HYPOXEMIA ,NEPRILYSIN ,CASPASES - Abstract
While gene mutations in the amyloid precursor protein (APP) and the presenilins lead to an accumulation of the amyloid β-peptide (Aβ) in the brain causing neurodegeneration and familial Alzheimer's disease (AD), over 95% of all AD cases are sporadic. Despite the pathologies being indistinguishable, relatively little is known about the mechanisms affecting generation of Aβ in the sporadic cases. Vascular disorders such as ischaemia and stroke are well established risk factors for the development of neurodegenerative diseases and systemic hypoxic episodes have been shown to increase Aβ production and accumulation. We have previously shown that hypoxia causes a significant decrease in the expression of the major Aβ-degrading enzyme neprilysin (NEP) which might deregulate Aβ clearance. Aβ itself is derived from the transmembrane APP along with several other biologically active metabolites including the C-terminal fragment (CTF) termed the APP intracellular domain (AICD), which regulates the expression of NEP and some other genes in neuronal cells. Here we show that in hypoxia there is a significantly increased expression of caspase-3, 8, and 9 in human neuroblastoma NB7 cells, which can degrade AICD. Using chromatin immunoprecipitation we have revealed that there was also a reduction of AICD bound to the NEP promoter region which underlies the decreased expression and activity of the enzyme under hypoxic conditions. Incubation of the cells with a caspase-3 inhibitor Z-DEVD-FMK could rescue the effect of hypoxia on NEP activity protecting the levels of AICD capable of binding the NEP promoter. These data suggest that activation of caspases might play an important role in regulation of NEP levels in the brain under pathological conditions such as hypoxia and ischaemia leading to a deficit of Aβ clearance and increasing the risk of development of AD. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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16. Herpes simplex virus type 1 infection in neurons leads to production and nuclear localization of APP intracellular domain (AICD): implications for Alzheimer's disease pathogenesis.
- Author
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Civitelli, Livia, Marcocci, Maria, Celestino, Ignacio, Piacentini, Roberto, Garaci, Enrico, Grassi, Claudio, De Chiara, Giovanna, and Palamara, Anna
- Subjects
HERPES simplex virus ,ALZHEIMER'S disease ,AMYLOID beta-protein precursor ,BIOACCUMULATION ,HUMAN phenotype ,PHOSPHORYLATION - Abstract
Several data indicate that neuronal infection with herpes simplex virus type 1 (HSV-1) causes biochemical alterations reminiscent of Alzheimer's disease (AD) phenotype. They include accumulation of amyloid-β (Aβ), which originates from the cleavage of amyloid precursor protein (APP), and hyperphosphorylation of tau protein, which leads to neurofibrillary tangle deposition. HSV-1 infection triggers APP processing and drives the production of several fragments including APP intracellular domain (AICD) that exerts transactivating properties. Herein, we analyzed the production and intracellular localization of AICD following HSV-1 infection in neurons. We also checked whether AICD induced the transcription of two target genes, neprilysin ( nep) and glycogen synthase kinase 3β ( gsk3β), whose products play a role in Aβ clearance and tau phosphorylation, respectively. Our data indicate that HSV-1 led to the accumulation and nuclear translocation of AICD in neurons. Moreover, results from chromatin immunoprecipitation assay showed that AICD binds the promoter region of both nep and gsk3β. Time course analysis of NEP and GSK3β expression at both mRNA and protein levels demonstrated that they are differently modulated during infection. NEP expression and enzymatic activity were initially stimulated but, with the progression of infection, they were down-regulated. In contrast, GSK3β expression remained nearly unchanged, but the analysis of its phosphorylation suggests that it was inactivated only at later stages of HSV-1 infection. Thus, our data demonstrate that HSV-1 infection induces early upstream events in the cell that may eventually lead to Aβ deposition and tau hyperphosphorylation and further suggest HSV-1 as a possible risk factor for AD. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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17. Role of caspase-3 in regulation of the amyloid-degrading neuropeptidase neprilysin level in the rat cortex after hypoxia
- Author
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Kozlova, D. I., Vasylev, D. S., Dubrovskaya, N. M., Nalivaeva, N. N., Tumanova, N. L., and Zhuravin, I. А.
- Published
- 2015
- Full Text
- View/download PDF
18. Racial Disparities in Cardiovascular Care: A Review of Culprits and Potential Solutions
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Capers, IV, Quinn and Sharalaya, Zarina
- Published
- 2014
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19. Lipid rafts and Alzheimr's disease: protein-lipid interactions and perturnation of signaling.
- Author
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Hicks, David A., Nalivaeva, Natalia N., and Tumer, Anthony J.
- Subjects
LIPID rafts ,PROTEIN-lipid interactions ,ALZHEIMER'S disease ,SPHINGOLIPIDS ,PROTEIN-protein interactions ,AXONAL transport ,LIGAND binding (Biochemistry) - Abstract
Lipid rafts are membrane domains, more ordered than the bulk membrane and enriched in cholesterol and sphingolipids. They represent a platform for protein-lipid and protein-protein interactions and for cellular signaling events. In addition to their normal functions, including membrane trafficking, ligand binding (including viruses), axonal development and maintenance of synaptic integrity, rafts have also been implicated in the pathogenesis of several neurodegenerative diseases including Alzheimer's disease (AD). Lipid rafts promote interaction of the amyloid precursor protein (APP) with the secretase (BACE-1) responsible for generation of the amyloid β peptide, Aβ. Rafts also regulate cholinergic signaling as well as acetylcholinesterase and Aβ interaction. In addition, such major lipid raft components as cholesterol and GM1 ganglioside have been directly implicated in pathogenesis of the disease. Perturbation of lipid raft integrity can also affect various signaling pathways leading to cellular death and AD. In this review, we discuss modulation of APP cleavage by lipid rafts and their components, while also looking at more recent findings on the role of lipid rafts in signaling events. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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20. Effect of Sodium Valproate Administration on Brain Neprilysin Expression and Memory in Rats.
- Author
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Nalivaeva, Natalia, Belyaev, Nikolai, Lewis, David, Pickles, Alasdair, Makova, Natalia, Bagrova, Daria, Dubrovskaya, Nadezhda, Plesneva, Svetlana, Zhuravin, Igor, and Turner, Anthony
- Abstract
Alzheimer's disease (AD) is accompanied by memory loss due to neuronal cell death caused by toxic amyloid β-peptide (Aβ) aggregates. In the healthy brain, a group of amyloid-degrading enzymes including neprilysin (NEP) maintain Aβ levels at physiologically low concentrations but, with age and under some pathological conditions, expression and activity of these enzymes decline predisposing to late-onset AD. Hence, up-regulation of NEP might be a viable strategy for prevention of Aβ accumulation and development of the disease. As we have recently shown, inhibitors of histone deacetylases, in particular, valproic acid (VA), were capable of up-regulating NEP expression and activity in human neuroblastoma SH-SY5Y cell lines characterised by very low levels of NEP. In the present study, analysing the effect of i.p. injections of VA to rats, we have observed up-regulation of expression and activity of NEP in rat brain structures, in particular, in the hippocampus. This effect was brain region- and age-specific. Administration of VA has also restored NEP activity and memory deficit in adult rats caused by prenatal hypoxia. This suggests that VA and more specific HDAC inhibitors can be considered as potential pharmaceutical agents for up-regulation of NEP activity and improvement of cognitive functions of ageing brain. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
21. γ-Secretase-Mediated Regulation of Neprilysin: Influence of Cell Density and Aging and Modulation by Imatinib.
- Author
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Bauer, Charlotte, Pardossi-Piquard, Raphaëlle, Dunys, Julie, Roy, Maggie, and Checler, Frédéric
- Subjects
ALZHEIMER'S disease ,CELLULAR aging ,AMYLOID beta-protein ,PRESENILINS ,ENZYMES ,FIBROBLASTS - Abstract
Proteolytic degradation has emerged as a key pathway involved in controlling levels of the Alzheimer's disease (AD)-associated amyloid-β peptides (Aβ) in the brain. The ectopeptidase, neprilysin (NEP), has been reported as the major Aβ-degrading enzyme in mice and human brains. We have previously shown that NEP expression and activity are regulated by AICD, the intracellular domain of the amyloid-β protein precursor (AβPP) generated by γ-secretase. Thus, NEP transcription, expression, and enzymatic activity are dramatically reduced in fibroblasts devoid of AβPP (the precursor of AICD) or lacking both presenilin (PS) 1 and 2 (two parent proteins contributing to AICD formation). We demonstrate here that NEP expression and activity are influenced by a number of cell passages and density, and we confirm a drastic reduction of NEP expression and activity in AβPP and PS null fibroblasts examined at similar passages and cell densities. Furthermore, Imatinib (Gleevec), a known tyrosine kinase inhibitor was recently shown to elevate AICD in H4 human neuroglioma cells, and this was accompanied by concomitant increases of NEP protein, mRNA levels, and activity. However, the demonstration of a causal link between Imatinib and AICD levels was still lacking. We show here an Imatinib-dependent effect on NEP expression and activity in murine fibroblasts and establish that Imatinib-induced modulation of NEP was abolished by the depletion of AβPP or its homologues APLP1 and APLP2, thereby confirming that Imatinib-mediated control of NEP could indeed be accounted for its effect on AICD. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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22. TLR4/MYD88-dependent, LPS-induced synthesis of PGE2 by macrophages or dendritic cells prevents anti-CD3-mediated CD95L upregulation in T cells.
- Author
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Weinlich, R, Bortoluci, K R, Chehab, C F, Serezani, C H, Ulbrich, A G, Peters-Golden, M, Russo, M, and Amarante-Mendes, G P
- Subjects
T cells ,ANTIGEN presenting cells ,CYTOKINES ,CELL proliferation ,ENDOTOXINS ,PROSTAGLANDINS - Abstract
Antigen-presenting cells (APCs) control T-cell responses by multiple mechanisms, including the expression of co-stimulatory molecules and the production of cytokines and other mediators that control T-cell proliferation, survival and differentiation. Here, we demonstrate that soluble factor(s) produced by Toll-like receptor (TLR)-activated APCs suppress activation-induced cell death (AICD). This effect was observed in non-stimulated APCs, but it was significantly increased after lipopolysaccharide (LPS) treatment. Using different KO mice, we found that the LPS-induced protective factor is dependent on TLR4/MyD88. We identified the protective factor as prostaglandin E
2 (PGE2 ) and showed that both APC-derived supernatants and PGE2 prevented CD95L upregulation in T cells in response to TCR/CD3 stimulation, thereby avoiding both AICD and activated T cell killing of target macrophages. The PGE2 receptors, EP2 and EP4, appear to be involved since pharmacological stimulation of these receptors mimics the protective effect on T cells and their respective antagonists interfere with the protection induced by either APCs derived or synthetic PGE2 . Finally, the engagement of EP2 and EP4 synergistically activates protein kinase A (PKA) and exchange protein directly activated by cAMP pathways to prevent AICD. Taken together, these results indicate that APCs can regulate T-cell levels of CD95L by releasing PGE2 in response to LPS through a TLR4/MyD88-dependent pathway, with consequences for both T cell and their own survival.Cell Death and Differentiation (2008) 15, 1901–1909; doi:10.1038/cdd.2008.128; published online 26 September 2008 [ABSTRACT FROM AUTHOR]- Published
- 2008
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23. Immunization with autologous T cells enhances in vivo anti-tumor immune responses accompanied by up-regulation of GADD45β.
- Author
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Li Wang, Fang Du, Qi Cao, Huiming Sheng, Baihua Shen, Yan Zhang, Yingna Diao, Jingwu Zhang, and Ningli Li
- Subjects
IMMUNIZATION ,T cells ,LABORATORY mice ,TUMOR immunology ,IMMUNE response ,AUTOIMMUNE diseases ,PHENOTYPES - Abstract
Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreactive T cells, which are involved in autoimmune diseases. However, it is unknown whether attenuated activated healthy autologous T-cell immunization could increase anti-tumor immune responses. To this end, C57Bl/6 mice were immunized with attenuated activated autologous T cells. The splenocytes from immunized mice showed a higher proliferative ability than that from naive mice. The special phenotype analysis showed that there were more CD8+ T cells and CD62L+ T cells in immunized mice after 24 h of culture with 10% fetal calf serum complete medium in vitro (P<0.01). These results demonstrated that this immunization may activate T cells in vivo. Furthermore, the splenocytes from immunized mice revealed resistance to activation-induced cell death (AICD) in vitro. To further study the relative genes that are responsible for the higher proliferation and resistance to AICD, the expression of Fas/Fas ligand (FasL) and GADD45b was measured by real-time PCR. The results indicated that GADD45β transcription was higher in the splenocytes from immunized mice than that in the naive mice. In addition, the Fas expression showed a parallel higher, but FasL did not change obviously. To investigate the biologic functions induced by immunization in vivo, a tumor model was established by EL-4 tumor cell inoculation in C57/Bl mice. Mice receiving autologous T-cell immunization had significantly inhibited tumor growth in vivo (P<0.01). This study implicated that immunization with attenuated activated autologous T cells enhances anti-tumor immune responses that participate in tumor growth inhibition.Cell Research (2006) 16: 702–712. doi:10.1038/sj.cr.7310083; published online 4 July 2006 [ABSTRACT FROM AUTHOR]
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- 2006
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24. MEF2C mediates the activation induced cell death (AICD) of macrophages.
- Author
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Wenxia Fu, Jinxue Wei, and Jun Gu
- Subjects
CELL death ,APOPTOSIS ,IMMUNE response ,MACROPHAGES ,LYMPHOCYTES ,SALMONELLA typhimurium - Abstract
Activation-induced cell death (AICD) of immune cells is widely believed to be crucial for the regulation of immune responses. Although macrophage apoptosis has been observed under a variety of pathological conditions, questions as to whether there is AICD of macrophages and how macrophage life span is regulated have not been well addressed. AICD in macrophages requires two signals. One is cell activation triggered by LPS or other bacterial components. The other is an event that exists in AICD-susceptible (primed) but not unsusceptible (resting) macrophages. Here we show that RAW264.7 cell is susceptible to LPS stimulation when it is primed with Salmonella typhimurium, type 5 adenovirus (Ad5) or IFN-γ. We found that the stability of the transcription factor MEF2C is increased in primed RAW264.7 cell. Transfection of a dominant negative form of MEF2C protects primed macrophage from cell death triggered by LPS. Our data demonstrate that the increase of MEF2C protein stability is a key factor in the AICD of macrophage.Cell Research (2006) 16: 559–565. doi:10.1038/sj.cr.7310073; published online 15 June 2006 [ABSTRACT FROM AUTHOR]
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- 2006
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25. Anti-TOSO antibody treatment promotes T cell activation-induced cell death (AICD) in vitro and in vivo
- Author
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Tan, Yi, Han, Xue, Wu, Xiaoran, Xing, Qiao, Chen, Lieping, and Wang, Shengdian
- Published
- 2014
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26. Characterization of amyloid-β precursor protein intracellular domain-associated transcriptional complexes in SH-SY5Y neurocytes
- Author
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Yang, Wulin, Lau, Amy Yong Chen, Luo, Shuizhong, Zhu, Qian, and Lu, Li
- Published
- 2012
- Full Text
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27. Automatic Implantable Cardioverter/Defibrillator: Inadvertent Discharges During Permanent Pacemaker Magnet Tests.
- Author
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Kim, Soo G., Furman, Seymour, Matos, Jeffrey A., Waspe, Lawrence E., Brodman, Richard, and Fisher, John D.
- Subjects
IMPLANTABLE cardioverter-defibrillators ,ELECTRIC discharges ,ELECTRIC countershock ,CARDIAC pacing ,CARDIAC pacemakers - Abstract
A patient with an automatic impIantable cardioverter defibrillator (AICD)® received two inadvertent shacks when a magnet was placed over the pacer during a routine permanent pacer check. Analysis of the rhythm strip suggested that both patients' QRS complexes (133 beats/minute) and asynchronous pacer artifacts (70 beats/minute) were counted by the AICD sensing system and exceeded the rate criteria of 153 beats/minute. This resulted in shocks from the AICD during sinus rhythm at 133 beats/minute. To avoid possible inadvertent shocks, an AICD should be deactivated while a magnet is placed over the pacemaker during a permanent pacer check. [ABSTRACT FROM AUTHOR]
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- 1987
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28. Psychiatrische Störungen bei Patienten mit Automatischen Implantiertem Cardioverter Defibrillator (AICD)
- Author
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Godemann, Frank
- Published
- 2008
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29. Insulysin Cleaves the APP Cytoplasmic Fragment at Multiple Sites
- Author
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Venugopal, Chitra, Pappolla, Miguel A., and Sambamurti, Kumar
- Published
- 2007
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30. Ventricular Arrhythmias after LV Remodelling: Surgical Ventricular Restoration or ICD?
- Author
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Donato, M. Di, Sabatier, M., Dor, V., Buckberg, G., and The RESTORE Group
- Published
- 2005
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- View/download PDF
31. Aging-related Correlation of Insulin-degrading Enzyme with γ-Secretase-generated Products Involving Insulin and Glucose Levels in Transgenic Mice
- Author
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Hwang, Dae Y., Cho, Jung S., Kim, Chuel K., Shim, Sun B., Jee, Seung W., Lee, Su H., Seo, Su J., Cho, Joon Y., Lee, Seok H., and Kim, Yong K.
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- 2005
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32. The role of activation-induced cell death in the differentiation of T-helper-cell subsets
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Roberts, Arthur I., Devadas, Satish, Zhang, Xiaoren, Zhang, Liying, Keegan, Achsah, Greeneltch, Kristy, Solomon, Jennifer, Wei, Lixin, Das, Jyoti, Sun, Erwei, Liu, Catherine, Yuan, Zengrong, Zhou, Jian-nian, and Shi, Yufang
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- 2003
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33. Isolated myocardial noncompaction als seltene Ursache einer Synkope im Kindesalter: Fallbericht mitLiteraturübersicht
- Author
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Binz, G., Knirsch, W., Lewin, M. A. G., Haas, N. A., Koch, W., Kandolf, R., and Uhlemann, F.
- Published
- 2003
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34. APP Processing Enzymes (Secretases) as Therapeutic Targets: Insights from the Use of Transgenics (Tgs) and Transfected Cells
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Marks, Neville and Berg, Martin J.
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- 2003
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35. Nuclear signaling: A common function of presenilin substrates?
- Author
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Steiner, Harald and Haass, Christian
- Published
- 2001
- Full Text
- View/download PDF
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