15 results on '"Li, Junying"'
Search Results
2. ATAC-seq and RNA-seq analysis unravel the mechanism of sex differentiation and infertility in sex reversal chicken
- Author
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Zhang, Xiuan, Li, Jianbo, Wang, Xiqiong, Jie, Yuchen, Sun, Congjiao, Zheng, Jiangxia, Li, Junying, Yang, Ning, and Chen, Sirui
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- 2023
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3. The m6A methylation regulates gonadal sex differentiation in chicken embryo
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Li, Jianbo, Zhang, Xiuan, Wang, Xiqiong, Sun, Congjiao, Zheng, Jiangxia, Li, Junying, Yi, Guoqiang, and Yang, Ning
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- 2022
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4. Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens (Gallus gallus).
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Nie, Ruixue, Zhang, Wenhui, Tian, Haoyu, Li, Junying, Ling, Yao, Zhang, Bo, Zhang, Hao, and Wu, Changxin
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CHICKENS ,OVULATION ,OVARIAN follicle ,AGRICULTURAL egg production ,ZONA pellucida ,PLASMINOGEN activators ,LACTATE dehydrogenase - Abstract
Background: In poultry, the smooth transition of follicles from the preovulatory-to-postovulatory phase impacts egg production in hens and can benefit the poultry industry. However, the regulatory mechanism underlying follicular ovulation in avians is a complex biological process that remains unclear. Results: Critical biochemical events involved in ovulation in domestic chickens (Gallus gallus) were evaluated by transcriptomics, proteomics, and in vitro assays. Comparative transcriptome analyses of the largest preovulatory follicle (F1) and postovulatory follicle (POF1) in continuous laying (CL) and intermittent laying (IL) chickens indicated the greatest difference between CL_F1 and IL_F1, with 950 differentially expressed genes (DEGs), and the smallest difference between CL_POF1 and IL_POF1, with 14 DEGs. Additionally, data-independent acquisition proteomics revealed 252 differentially abundant proteins between CL_F1 and IL_F1. Perivitelline membrane synthesis, steroid biosynthesis, lysosomes, and oxidative phosphorylation were identified as pivotal pathways contributing to ovulation regulation. In particular, the regulation of zona pellucida sperm-binding protein 3, plasminogen activator, cathepsin A, and lactate dehydrogenase A (LDHA) was shown to be essential for ovulation. Furthermore, the inhibition of LDHA decreased cell viability and promoted apoptosis of ovarian follicles in vitro. Conclusions: This study reveals several important biochemical events involved in the process of ovulation, as well as crucial role of LDHA. These findings improve our understanding of ovulation and its regulatory mechanisms in avian species. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Identification of Duplication Genotypes of the Feathering Rate Gene in Chicken by a Multiplex PCR Following Electrophoresis and/or Sanger Sequencing.
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Shen, Qingmiao, Li, Junying, Bao, Haigang, and Wu, Changxin
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CHICKEN breeds , *POLYMERASE chain reaction , *DIAGNOSTIC use of polymerase chain reaction , *POULTRY breeding , *FEATHERS , *GENOTYPES , *PHENOTYPES - Abstract
Simple Summary: Autosexing based on sex-linked phenotypes of late feathering (LF) and early feathering (EF) was widely used in poultry production. Previous studies found that tandem duplication on the Z chromosome is responsible for LF. In this study, based on tandem duplication, we developed a multiplex PCR test for genotyping through reaction optimization. The genotyping result for feathering rate with the multiplex PCR test was consistent with the sex and phenotype records, and the result of homozygote and heterozygote tests of LF males were verified by test cross and tallied with offspring's phenotypes. The multiplex PCR test is stable and accurate for feathering rate genotyping, applicable to all LF chickens associated with tandem duplication, and has great significance for poultry breeding. Sex-linked phenotypes of late feathering (LF) and early feathering (EF) are controlled by a pair of alleles K and k+. Autosexing based on the feathering rate is widely used in poultry production. It is reported that a tandem duplication of 176,324 base pairs linked to the K locus is responsible for LF expression and could be used as a molecular marker to detect LF chicken. So far, there is no genotyping method that can accurately and stably identify the LF homozygote and heterozygote in all chicken breeds. In the present study, a multiplex PCR test was developed to identify EF, LF homozygote, and heterozygote according to electrophoretic bands and the relative height of the peaks by Sanger sequencing. We tested 413 chickens of six native Chinese breeds with this method. The identification was consistent with the sex and phenotype records of the chickens. Band density analysis was performed, and the results supported our genotyping using the new assay. In order to further verify the accuracy of this test in distinguishing homozygote and heterozygote males, 152 LF males were mated with EF females, and the results of the offspring's phenotypes were consistent with our expectations. Our results support tandem duplication as molecular markers of LF, and this new test is applicable to all LF chickens associated with tandem duplication. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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6. Temporal Expression of Myogenic Regulatory Genes in Different Chicken Breeds during Embryonic Development.
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Gu, Shuang, Wen, Chaoliang, Li, Junying, Liu, Honghong, Huang, Qiang, Zheng, Jiangxia, Sun, Congjiao, and Yang, Ning
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POULTRY breeding ,EMBRYOLOGY ,REGULATOR genes ,MUSCLE growth ,CHICKEN breeds ,GENE expression ,MYOBLASTS - Abstract
The basic units of skeletal muscle in all vertebrates are multinucleate myofibers, which are formed from the fusion of mononuclear myoblasts during the embryonic period. In order to understand the regulation of embryonic muscle development, we selected four chicken breeds, namely, Cornish (CN), White Plymouth Rock (WPR), White Leghorn (WL), and Beijing-You Chicken (BYC), for evaluation of their temporal expression patterns of known key regulatory genes (Myomaker, MYOD, and MSTN) during pectoral muscle (PM) and thigh muscle (TM) development. The highest expression level of Myomaker occurred from embryonic days E13 to E15 for all breeds, indicating that it was the crucial stage of myoblast fusion. Interestingly, the fast-growing CN showed the highest gene expression level of Myomaker during the crucial stage. The MYOD gene expression at D1 was much higher, implying that MYOD might have an important role after hatching. Histomorphology of PM and TM suggested that the myofibers was largely complete at E17, which was speculated to have occurred because of the expression increase in MSTN and the expression decrease in Myomaker. Our research contributes to lay a foundation for the study of myofiber development during the embryonic period in different chicken breeds. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Exogenous cMHM regulates the expression of DMRT1 and ERα in avian testes
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Yang, Xiurong, Zheng, Jiangxia, Xu, Guiyun, Qu, Lujiang, Chen, Sirui, Li, Junying, and Yang, Ning
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- 2010
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8. Research of blastocyte-like structure in chicken
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Li, Jia, Pan, Qiuzhen, Li, Junying, Han, Hongbing, Sun, Shufeng, Yang, Jun, Xu, Shuguang, Tian, Liang, Lian, Zhengxing, Yang, Ning, and Li, Ning
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- 2005
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9. Genetic basis of chicken plumage color in artificial population of complex epistasis.
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Hua, Guoying, Chen, Jianfei, Wang, Jiankui, Li, Junying, and Deng, Xuemei
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COLOR of birds ,GENOME-wide association studies ,FEATHERS ,LEGHORN chicken ,POULTRY breeding ,SINGLE nucleotide polymorphisms ,RECESSIVE genes ,CHICKENS - Abstract
Summary: Chicken plumage color, the genetic basis of which is often affected by epistasis, has long interested scientists. In the current study, a population of complex epistasis was constructed by crossing dominant White Leghorn chickens with recessive white feather chickens. Through a genome‐wide association study, we identified single nucleotide polymorphisms and genes significantly associated with white and colored plumage in hens at different developmental stages. Interestingly, white plumage in adulthood was associated with the recessive white feather gene (TYR), whereas white feathers at birth stage were associated with the dominant white feather gene (PMEL), indicating age‐related roles for these genes. TYR was shown to exert an epistatic effect on PMEL in adult hens. Additionally, TYR had an epistatic effect on barred plumage, while barred plumage had an epistatic effect on black plumage. TYR had no epistatic effect on the yellow plumage. We confirmed that the barred plumage gene is CDKN2A, as reported in previous studies. Golgb1 and REEP3, which play important roles in the Golgi network and affect the formation of feather pigments, are important candidate genes for yellow plumage. The candidate genes for black plumage are CAMKK1 and IFT22. Further research is warranted to elucidate the molecular mechanisms underlying these traits. [ABSTRACT FROM AUTHOR]
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- 2021
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10. Genetic Diversity of MHC B-F/B-L Region in 21 Chicken Populations.
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Yuan, Yiming, Zhang, Huanmin, Yi, Guoqiang, You, Zhen, Zhao, Chunfang, Yuan, Haixu, Wang, Kejun, Li, Junying, Yang, Ning, and Lian, Ling
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GENETIC variation ,MAJOR histocompatibility complex ,GENETIC models ,SINGLE nucleotide polymorphisms ,CHICKENS ,CHICKEN diseases ,POULTRY breeding - Abstract
The chicken major histocompatibility complex (MHC) on chromosome 16 is the most polymorphic region across the whole genome, and also an ideal model for genetic diversity investigation. The MHC B-F/B-L region is 92 kb in length with high GC content consisting of 18 genes and one pseudogene (Blec4), which plays important roles in immune response. To evaluate polymorphism of the Chinese indigenous chickens as well as to analyze the effect of selection to genetic diversity, we used WaferGen platform to identify sequence variants of the B-F/B-L region in 21 chicken populations, including the Red Jungle Fowl (RJF), Cornish (CS), White Leghorns (WLs), 16 Chinese domestic breeds, and two well-known inbred lines 6
3 and 72 . A total of 3,319 single nucleotide polymorphism (SNPs) and 181 INDELs in the B-F/B-L region were identified among 21 populations, of which 2,057 SNPs (62%) and 159 INDELs (88%) were novel. Most of the variants were within the intron and the flanking regions. The average variation density was 36 SNPs and 2 INDELs per kb, indicating dramatical high diversity of this region. Furthermore, BF2 was identified as the hypervariable genes with 67 SNPs per kb. Chinese domestic populations showed higher diversity than the WLs and CS. The indigenous breeds, Nandan Yao (NY), Xishuangbanna Game (XG), Gushi (GS), and Xiayan (XY) chickens, were the top four with the highest density of SNPs and INDELs. The highly inbred lines 63 and 72 have the lowest diversity, which might be resulted from a long-term intense selection for decades. Collectively, we refined the genetic map of chicken MHC B-F/B-L region, and illustrated genetic diversity of 21 chicken populations. Abundant genetic variants were identified, which not only strikingly expanded the current Ensembl SNP database, but also provided comprehensive data for researchers to further investigate association between variants in MHC and immune traits. [ABSTRACT FROM AUTHOR]- Published
- 2021
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11. Genomic Analysis Reveals Pleiotropic Alleles at EDN3 and BMP7 Involved in Chicken Comb Color and Egg Production.
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Dong, Xianggui, Li, Junying, Zhang, Yuanyuan, Han, Deping, Hua, Guoying, Wang, Jiankui, Deng, Xuemei, and Wu, Changxin
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AGRICULTURAL egg production ,BREEDING ,CHICKENS ,ALLELES ,POULTRY breeding ,CHICKEN diseases ,LOCUS (Genetics) - Abstract
Artificial selection is often associated with numerous changes in seemingly unrelated phenotypic traits. The genetic mechanisms of correlated phenotypes probably involve pleiotropy or linkage of genes related to such phenotypes. Dongxiang blue-shelled chicken, an indigenous chicken breed of China, has segregated significantly for the dermal hyperpigmentation phenotype. Two lines of the chicken have been divergently selected with respect to comb color for over 20 generations. The red comb line chicken produces significantly higher number of eggs than the dark comb line chicken. The objective of this study was to explore potential mechanisms involved in the relationship between comb color and egg production among chickens. Based on the genome-wide association study results, we identified a genomic region on chromosome 20 involving EDN3 and BMP7 , which is associated with hyperpigmentation of chicken comb. Further analyses by selection signatures in the two divergent lines revealed that several candidate genes, including EDN3 , BMP7 , BPIFB3 , and PCK1 , closely located on chromosome 20 are involved in the development of neural crest cell and reproductive system. The two genes EDN3 and BMP7 have known roles in regulating both ovarian function and melanogenesis, indicating the pleiotropic effect on hyperpigmentation and egg production in blue-shelled chickens. Association analysis for egg production confirmed the pleiotropic effect of selected loci identified by selection signatures. The study provides insights into phenotypic evolution due to genetic variation across the genome. The information might be useful in the current breeding efforts to develop improved breeds for egg production. [ABSTRACT FROM AUTHOR]
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- 2019
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12. T329S Mutation in the FMO3 Gene Alleviates Lipid Metabolic Diseases in Chickens in the Late Laying Period.
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Song, Jianlou, Huang, Mingyi, Shi, Xuefeng, Li, Xianyu, Chen, Xia, He, Zhaoxiang, Li, Junying, Xu, Guiyun, and Zheng, Jiangxia
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CHICKEN diseases ,METABOLIC disorders ,LIPID metabolism ,HENS ,FATTY liver ,GENETIC mutation - Abstract
Simple Summary: The lipid deposition and health status of egg-laying hens is crucial to the development of the poultry industry. This study aimed to evaluate the effects of genetic variations in the flavin-containing monooxygenase 3 (FMO3) on the lipid metabolic diseases of laying hens during the late laying period. The results showed that the T329S mutation in FMO3 moderated the lipid parameters and decreased the atherosclerotic lesions and hepatic steatosis in laying hens with homozygous T329S mutation. In conclusion, the T329S mutation in FMO3 is closely associated with the improvement of lipid metabolic diseases in laying hens during the late laying period. The results of this study may contribute to overcoming the challenge of lipid metabolic diseases in laying hens during the late laying period. The T329S mutation in flavin-containing monooxygenase 3 (FMO3) impairs the trimethylamine (TMA) metabolism in laying hens. The TMA metabolic pathway is closely linked to lipid metabolic diseases, such as atherosclerosis and fatty liver disease. We aimed to evaluate the effects of the T329S mutation in FMO3 on lipid metabolism in chickens during the late laying period. We selected 18 FMO3 genotyped individuals (consisting of six AA, six AT, and six TT hens) with similar body weight and production performance. The lipid metabolism and deposition characteristics of the laying hens with different genotypes were compared. The T329S mutation moderated the serum-lipid parameters in TT hens compared to those in AA and AT hens from 49 to 62 weeks. Furthermore, it reduced the serum trimethylamine N-oxide concentrations and increased the serum total bile acid (p < 0.05) and related lipid transporter levels in TT hens. Moreover, it significantly (p < 0.01) decreased atherosclerotic lesions and hepatic steatosis in TT hens compared to those in the AA and AT hens. Our findings may help improve the health status in laying hens during the late laying period. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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13. Androgen Inhibits Abdominal Fat Accumulation and Negatively Regulates the PCK1 Gene in Male Chickens.
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Duan, Jinlin, Shao, Fan, Shao, Yonggang, Li, Junying, Ling, Yao, Teng, Kedao, Li, Hongwei, and Wu, Changxin
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ANDROGENS ,ABDOMINAL adipose tissue ,GENETIC regulation ,CAPONS & caponizing ,TESTIS surgery ,MOLECULAR biology ,MICROARRAY technology ,LIPOGENESIS in poultry - Abstract
Capons are male chickens whose testes have been surgically incised. Capons show a significant increase in fat accumulation compared to intact male chickens. However, while caponization leads to a significant reduction in androgen levels in roosters, little is known about the molecular mechanisms through which androgen status affects lipogenesis in avian species. Therefore, investigation of the influence of androgens on fat accumulation in the chicken will provide insights into this process. In this study, Affymetrix microarray technology was used to analyze the gene expression profiles of livers from capons and intact male chickens because the liver is the major site of lipogenesis in avian species. Through gene ontology, we found that genes involved in hepatic lipogenic biosynthesis were the most highly enriched. Interestingly, among the upregulated genes, the cytosolic form of the phosphoenolpyruvate carboxykinase (PCK1) gene showed the greatest fold change. Additionally, in conjunction with quantitative real-time PCR data, our results suggested that androgen status negatively regulated the PCK1 gene in male chickens. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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14. Variations in seminal microbiota and their functional implications in chickens adapted to high-altitude environments.
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Jiang, Xinwei, Zhang, Boxuan, Gou, Qinli, Cai, Ronglang, Sun, Congjiao, Li, Junying, Yang, Ning, and Wen, Chaoliang
- Abstract
Seminal fluid, once believed to be sterile, is now recognized as constituting a complex and dynamic environment inhabited by a diverse community of micro-organisms. However, research on the seminal microbiota in chickens is limited, and microbiota variations among different chicken breeds remain largely unexplored. In this study, we collected semen samples from Beijing You Chicken (BYC) and Tibetan Chicken (TC) and explored the characteristics of the microbiota using 16S rRNA gene sequencing. Additionally, we collected cloacal samples from the TC to control for environmental contamination. The results revealed that the microbial communities in the semen were significantly different from those in the cloaca. Firmicutes and Actinobacteriota were the predominant phyla in BYC and TC semen, respectively, with Lactobacillus and Phyllobacterium being the dominant genera in each group. Additionally, the seminal microbiota of BYC exhibited greater richness and evenness than that of TC. Principal coordinate analysis (PCoA) indicated significant intergroup differences between the seminal microbiotas of BYC and TC. Subsequently, by combining linear discriminant analysis effect size and random forest analyses, we identified Lactobacillus as the predominant microorganism in BYC semen, whereas Phyllobacterium dominated in TC semen. Furthermore, co-occurrence network analysis revealed a more intricate network in the BYC group than in the TC group. Additionally, unique microbial functional characteristics were observed in each breed, with TC exhibiting metabolic features potentially associated with their ability to adapt to high-altitude environments. The results of this study emphasized the unique microbiota present in chicken semen, which may be influenced by genetics and evolutionary history. Significant variations were observed between low-altitude and high-altitude breeds, highlighting the breed-specific implications of the seminal microbiota for reproduction and high-altitude adaptation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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15. Genomic and transcriptomic analyses reveal genetic adaptation to cold conditions in the chickens.
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Zhao, Xiurong, Zhang, Jinxin, Wang, Huie, Li, Haiying, Qu, Changqing, Wen, Junhui, Zhang, Xinye, Zhu, Tao, Nie, Changsheng, Li, Xinghua, Muhatai, Gemingguli, Wang, Liang, Lv, XueZe, Yang, Weifang, Zhao, Chunjiang, Bao, Haigang, Li, Junying, Zhu, Bo, Cao, Guomin, and Xiong, Wenjie
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COLD adaptation , *GENOMICS , *CHICKEN breeds , *DISTRIBUTION (Probability theory) , *CHICKENS , *WHOLE genome sequencing , *CHICKEN embryos , *HOMEOBOX genes - Abstract
Under the pressure of natural and artificial selection, domestic animals, including chickens, have evolved unique mechanisms of genetic adaptations such as high-altitude adaptation, hot and arid climate adaptation, and desert adaptation. Here, we investigated the genetic basis of cold tolerance in chicken by integrating whole-genome and transcriptome sequencing technologies. Genome-wide comparative analyses of 118 chickens living in different latitudes showed 46 genes and several pathways that may be involved in cold adaptation. The results of the functional enrichment analysis of differentially expressed genes proved the important role of metabolic pathways and immune-related pathways in cold tolerance in chickens. The subsequent integration of whole genome and transcriptome sequencing technology further identified six genes — dnah5 (dynein axonemal heavy chain 5), ptgs2 (prostaglandin-endoperoxide synthase 2), inhba (inhibin beta A subunit), irx2 (iroquois homeobox 2), ensgalg00000054917 , and ensgalg00000046652 — requiring more detailed studies. In addition, we also discovered different allele frequency distributions of five SNPs (single nucleotide polymorphisms) within ptgs2 and nine SNPs within dnah5 in chickens in different latitudes, suggesting strong selective pressure of these two genes in chickens. We provide a novel insight into the genetic adaptation in chickens to cold environments, and provide a reference for evaluating and developing adaptive chicken breeds in cold environments. • The genetic basis of cold tolerance in indigenous chickens long-living in the cold environment were explored. • Two important genes associated with cold tolerance were identified. • Provide a precious source for evaluating and developing adaptive chicken breeds to face climate change. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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