8 results on '"Barreda-Manso, M. Asunción"'
Search Results
2. Cannabinoid CB2 receptors in the mouse brain: relevance for Alzheimer’s disease
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López, Alicia, Aparicio, Noelia, Pazos, M. Ruth, Grande, M. Teresa, Barreda-Manso, M. Asunción, Benito-Cuesta, Irene, Vázquez, Carmen, Amores, Mario, Ruiz-Pérez, Gonzalo, García-García, Elena, Beatka, Margaret, Tolón, Rosa M., Dittel, Bonnie N., Hillard, Cecilia J., and Romero, Julián
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- 2018
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3. TUDCA: An Agonist of the Bile Acid Receptor GPBAR1/TGR5 With Anti-Inflammatory Effects in Microglial Cells.
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Yanguas‐Casás, Natalia, Barreda‐Manso, M. Asunción, Nieto‐Sampedro, Manuel, and Romero‐Ramírez, Lorenzo
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BILE acids , *ANTI-inflammatory agents , *MICROGLIA , *TAUROURSODEOXYCHOLIC acid , *NEUROPROTECTIVE agents - Abstract
Bile acids are steroid acids found in the bile of mammals. The bile acid conjugate tauroursodeoxycholic acid (TUDCA) is neuroprotective in different animal models of stroke and neurological diseases. We have previously shown that TUDCA has anti-inflammatory effects on glial cell cultures and in a mouse model of acute neuroinflammation. We show now that microglial cells (central nervous system resident macrophages) express the G protein-coupled bile acid receptor 1/Takeda G protein-coupled receptor 5 (GPBAR1/TGR5) in vivo and in vitro. TUDCA binding to GPBAR1/TGR5 caused an increase in intracellular cAMP levels in microglia that induced anti-inflammatory markers, while reducing pro-inflammatory ones. This anti-inflammatory effect of TUDCA was inhibited by small interference RNA for GPBAR1/TGR5 receptor, as well as by treatment with a protein kinase A (PKA) inhibitor. In the mouse model of acute neuroinflammation, treating the animals with TUDCA was clearly anti-inflammatory. TUDCA biased the microglial phenotype in vivo and in vitro toward the anti-inflammatory. The bile acid receptor GPBAR1/TGR5 could be a new therapeutic target for pathologies coursing with neuroinflammation and microglia activation, such as traumatic brain injuries, stroke, or neurodegenerative diseases. TUDCA and other GPBAR1/TGR5 agonists need to be further investigated, to determine their potential in attenuating the neuropathologies associated with microglia activation. J. Cell. Physiol. 232: 2231-2245, 2017. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
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- 2017
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4. Neuroprotection and Blood-Brain Barrier Restoration by Salubrinal After a Cortical Stab Injury.
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Barreda‐Manso, M. Asunción, Yanguas‐Casás, Natalia, Nieto‐Sampedro, Manuel, and Romero‐Ramírez, Lorenzo
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NEUROPROTECTIVE agents , *STAB wounds , *BLOOD-brain barrier , *HOMEOSTASIS , *MICROGLIA , *THERAPEUTICS - Abstract
Following a central nervous system (CNS) injury, restoration of the blood-brain barrier (BBB) integrity is essential for recovering homeostasis. When this process is delayed or impeded, blood substances and cells enter the CNS parenchyma, initiating an additional inflammatory process that extends the initial injury and causes so-called secondary neuronal loss. Astrocytes and profibrotic mesenchymal cells react to the injury and migrate to the lesion site, creating a new glia limitans that restores the BBB. This process is beneficial for the resolution of the inflammation, neuronal survival, and the initiation of the healing process. Salubrinal is a small molecule with neuroprotective properties in different animal models of stroke and trauma to the CNS. Here, we show that salubrinal increased neuronal survival in the neighbourhood of a cerebral cortex stab injury. Moreover, salubrinal reduced cortical blood leakage into the parenchyma of injured animals compared with injured controls. Adjacent to the site of injury, salubrinal induced immunoreactivity for platelet-derived growth factor subunit B (PDGF-B), a specific mitogenic factor for mesenchymal cells. This effect might be responsible for the increased immunoreactivity for fibronectin and the decreased activation of microglia and macrophages in injured mice treated with salubrinal, compared with injured controls. The immunoreactivity for PDGF-B colocalized with neuronal nuclei (NeuN), suggesting that cortical neurons in the proximity of the injury were the main source of PDGF-B. Our results suggest that after an injury, neurons play an important role in both, the healing process and the restoration of the BBB integrity. J. Cell. Physiol. 232: 1501-1510, 2017. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
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- 2017
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5. Integrated Stress Response as a Therapeutic Target for CNS Injuries.
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Romero-Ramírez, Lorenzo, Nieto-Sampedro, Manuel, and Barreda-Manso, M. Asunción
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COMPLICATIONS of brain injuries ,CENTRAL nervous system injuries ,BIOLOGICAL models ,CELL physiology ,CEREBROVASCULAR disease ,CYTOKINES ,MOLECULAR biology ,SPINAL cord injuries ,PHYSIOLOGICAL stress ,DISEASE complications - Abstract
Central nervous system (CNS) injuries, caused by cerebrovascular pathologies or mechanical contusions (e.g., traumatic brain injury, TBI) comprise a diverse group of disorders that share the activation of the integrated stress response (ISR). This pathway is an innate protective mechanism, with encouraging potential as therapeutic target for CNS injury repair. In this review, we will focus on the progress in understanding the role of the ISR and we will discuss the effects of various small molecules that target the ISR on different animal models of CNS injury. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Salubrinal inhibits the expression of proteoglycans and favors neurite outgrowth from cortical neurons in vitro.
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Barreda-Manso, M. Asunción, Yanguas-Casás, Natalia, Nieto–Sampedro, Manuel, and Romero-Ramírez, Lorenzo
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PROTEOGLYCANS , *PROTEIN expression , *NEURAL physiology , *CELL growth , *IN vitro studies , *ASTROCYTES , *EXTRACELLULAR matrix proteins - Abstract
After CNS injury, astrocytes and mesenchymal cells attempt to restore the disrupted glia limitans by secreting proteoglycans and extracellular matrix proteins (ECMs), forming the so-called glial scar. Although the glial scar is important in sealing the lesion, it is also a physical and functional barrier that prevents axonal regeneration. The synthesis of secretory proteins in the RER is under the control of the initiation factor of translation eIF2α. Inhibiting the synthesis of secretory proteins by increasing the phosphorylation of eIF2α, might be a pharmacologically efficient way of reducing proteoglycans and other profibrotic proteins present in the glial scar. Salubrinal, a neuroprotective drug, decreased the expression and secretion of proteoglycans and other profibrotic proteins induced by EGF or TGFβ, maintaining eIF2α phosphorylated. Besides, Salubrinal also reduced the transcription of proteoglycans and other profibrotic proteins, suggesting that it induced the degradation of non-translated mRNA. In a model in vitro of the glial scar, cortical neurons grown on cocultures of astrocytes and fibroblasts with TGFβ treated with Salubrinal, showed increased neurite outgrowth compared to untreated cells. Our results suggest that Salubrinal may be considered of therapeutic value facilitating axonal regeneration, by reducing overproduction and secretion of proteoglycans and profibrotic protein inhibitors of axonal growth. [ABSTRACT FROM AUTHOR]
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- 2015
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7. Cannabinoid CB2 receptors in the mouse brain: relevance for Alzheimer's disease.
- Author
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López, Alicia, Aparicio, Noelia, Pazos, M. Ruth, Grande, M. Teresa, Barreda-Manso, M. Asunción, Benito-Cuesta, Irene, Vázquez, Carmen, Amores, Mario, Ruiz-Pérez, Gonzalo, García-García, Elena, Beatka, Margaret, Tolón, Rosa M., Dittel, Bonnie N., Hillard, Cecilia J., and Romero, Julián
- Subjects
CANNABINOID receptors ,ALZHEIMER'S disease ,GREEN fluorescent protein ,INFLAMMATION ,BRAIN stem - Abstract
Background: Because of their low levels of expression and the inadequacy of current research tools, CB2 cannabinoid receptors (CB2R) have been difficult to study, particularly in the brain. This receptor is especially relevant in the context of neuroinflammation, so novel tools are needed to unveil its pathophysiological role(s).Methods: We have generated a transgenic mouse model in which the expression of enhanced green fluorescent protein (EGFP) is under the control of the cnr2 gene promoter through the insertion of an Internal Ribosomal Entry Site followed by the EGFP coding region immediately 3' of the cnr2 gene and crossed these mice with mice expressing five familial Alzheimer's disease (AD) mutations (5xFAD).Results: Expression of EGFP in control mice was below the level of detection in all regions of the central nervous system (CNS) that we examined. CB2R-dependent-EGFP expression was detected in the CNS of 3-month-old AD mice in areas of intense inflammation and amyloid deposition; expression was coincident with the appearance of plaques in the cortex, hippocampus, brain stem, and thalamus. The expression of EGFP increased as a function of plaque formation and subsequent microgliosis and was restricted to microglial cells located in close proximity to neuritic plaques. AD mice with CB2R deletion exhibited decreased neuritic plaques with no changes in IL1β expression.Conclusions: Using a novel reporter mouse line, we found no evidence for CB2R expression in the healthy CNS but clear up-regulation in the context of amyloid-triggered neuroinflammation. Data from CB2R null mice indicate that they play a complex role in the response to plaque formation. [ABSTRACT FROM AUTHOR]- Published
- 2018
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8. Tauroursodeoxycholic acid reduces glial cell activation in an animal model of acute neuroinflammation.
- Author
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Yanguas-Casás, Natalia, Barreda-Manso, M Asunción, Nieto-Sampedro, Manuel, and Romero-Ramírez, Lorenzo
- Abstract
Background: Bile acids are steroid acids found predominantly in the bile of mammals. The bile acid conjugate tauroursodeoxycholic acid (TUDCA) is a neuroprotective agent in different animal models of stroke and neurological diseases. However, the anti-inflammatory properties of TUDCA in the central nervous system (CNS) remain unknown.Methods: The acute neuroinflammation model of intracerebroventricular (icv) injection with bacterial lipopolysaccharide (LPS) in C57BL/6 adult mice was used herein. Immunoreactivity against Iba-1, GFAP, and VCAM-1 was measured in coronal sections in the mice hippocampus. Primary cultures of microglial cells and astrocytes were obtained from neonatal Wistar rats. Glial cells were treated with proinflammatory stimuli to determine the effect of TUDCA on nitrite production and activation of inducible enzyme nitric oxide synthase (iNOS) and NFκB luciferase reporters. We studied the effect of TUDCA on transcriptional induction of iNOS and monocyte chemotactic protein-1 (MCP-1) mRNA as well as induction of protein expression and phosphorylation of different proteins from the NFκB pathway.Results: TUDCA specifically reduces microglial reactivity in the hippocampus of mice treated by icv injection of LPS. TUDCA treatment reduced the production of nitrites by microglial cells and astrocytes induced by proinflammatory stimuli that led to transcriptional and translational diminution of the iNOS. This effect might be due to inhibition of the NFκB pathway, activated by proinflammatory stimuli. TUDCA decreased in vitro microglial migration induced by both IFN-γ and astrocytes treated with LPS plus IFN-γ. TUDCA inhibition of MCP-1 expression induced by proinflammatory stimuli could be in part responsible for this effect. VCAM-1 inmunoreactivity in the hippocampus of animals treated by icv LPS was reduced by TUDCA treatment, compared to animals treated with LPS alone.Conclusions: We show a triple anti-inflammatory effect of TUDCA on glial cells: i) reduced glial cell activation, ii) reduced microglial cell migratory capacity, and iii) reduced expression of chemoattractants (e.g., MCP-1) and vascular adhesion proteins (e.g., VCAM-1) required for microglial migration and blood monocyte invasion to the CNS inflammation site. Our results present a novel TUDCA anti-inflammatory mechanism, with therapeutic implications for inflammatory CNS diseases. [ABSTRACT FROM AUTHOR]- Published
- 2014
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