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Norfloxacin (NFX) and sulfadiazine (SDZ) are two widely used antibiotics belonging to fluoroquinolone and sulfonamide groups, respectively, and have become the commonly detected micropollutants in aquatic environments. However, only few works have been conducted to investigate the highly probable inhibition of these antibiotic pollutants to Arthrospira platensis, which is an important species of cyanobacteria that is one of primary producers in aquatic ecosystems and should be remarkably sensitive to environmental pollutants due to its prokaryotic characteristics. Hence, the toxicological effects and removal efficiencies of NFX and SDZ in culturing A. platensis were studied by analyzing the biomass growth, photosynthetic pigments, primary biocomponents, and antibiotics concentration. The corresponding variations of these characteristics showed the higher sensitivity of A. platensis to NFX than to SDZ, indicating the specifically targeted effect of NFX on A. platensis, which could be confirmed in silico by the higher binding affinity of NFX with the critical enzyme. The obtained results illustrated the roles of NFX and SDZ on the growth of A. platensis, thus providing the great support in employing A. platensis to reduce hazards from contaminated water and recover biomass resources.

Discovering a sustainable route to ammonia as a fertiliser and as an energy carrier is critically important, but many recent reports on the electrochemical nitrogen reduction are false positives. Here the authors uncover the emerging experimental traps and detail protocols to reliably avoid them.

Abstract Background Cerebral palsy (CP) is a syndrome of childhood movement and posture disorders. Clinical evidence is still limited and sometimes inconclusive about the benefits of human umbilical cord mesenchymal stem cells (hUC-MSCs) for CP. We conducted a randomized trial to evaluate the safety and efficacy of hUC-MSC transplantation concomitant with rehabilitation in patients with CP. Methods Eligible patients were allocated into the hUC-MSC group and control group. In addition to rehabilitation, the patients in the hUC-MSC group received four transfusions of hUC-MSCs intravenously, while the control group received a placebo. Adverse events (AEs) were collected for safety evaluation in the 12-month follow-up phase. Primary endpoints were assessed as activities of daily living (ADL), comprehensive function assessment (CFA), and gross motor function measure (GMFM) scales. In addition, cerebral metabolic activity was detected by 18F-FDG-PET/CT to explore the possible mechanism of the therapeutic effects. Primary endpoint data were analyzed by ANOVA using SPSS version 20.0. Results Forty patients were enrolled, and 1 patient withdrew informed consent. Therefore, 39 patients received treatments and completed the scheduled assessments. No significant difference was shown between the 2 groups in AE incidence. Additionally, significant improvements in ADL, CFA, and GMFM were observed in the hUC-MSC group compared with the control group. In addition, the standard uptake value of 18F-FDG was markedly increased in 3 out of 5 patients from the hUC-MSC group at 12 months after transplantation. Conclusions Our clinical data showed that hUC-MSC transplantation was safe and effective at improving the gross motor and comprehensive function of children with CP when combined with rehabilitation. Recovery of cerebral metabolic activity might play an essential role in the improvements in brain function in patients with CP. The therapeutic window, transfusion route, and dosage in our study were considerable for reference in clinical application. Trial registration Chictr.org.cn, ChiCTR1800016554. Registered 08 June 2018—retrospectively registered. The public title was “Randomized trial of umbilical cord-derived mesenchymal stem cells for cerebral palsy.”

Background: The incidence of cerebral ischemia disease leading cause of death in human population worldwide. Treatment of cerebral ischemia remains a clinical challenge for researchers and mechanisms of cerebral ischemia remain unknown. During the cerebral ischemia, inflammatory reaction and oxidative stress plays an important role. The current investigation scrutinized the neuroprotective and anti-inflammatory role of pterostilbene against cerebral ischemia in middle cerebral artery occlusion (MCAO) rodent model and explore the underlying mechanism.Methods: The rats were divided into following groups viz., normal, sham, MCAO and MCAO + pterostilbene (25 mg/kg) group, respectively. The groups received the oral administration of pterostilbene for 30 days followed by MCAO induction. The neurological score, brain water content, infarct volume and Evan blue leakage were estimated. Hepatic, renal, heart, inflammatory cytokines and inflammatory mediators were estimated.Results: Pterostilbene treatment significantly (p < 0.001) improved the body weight and suppressed the glucose level and brain weight. Pterostilbene significantly (p < 0.001) reduced the hepatic, renal and heart parameters. Pterostilbene significantly (p < 0.001) decreased the level of glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and decreased the level of malonaldehyde (MDA), 8-Hydroxy-2′-deoxyguanosine (8-OHdG). Pterostilbene significantly (p < 0.001) inflammatory cytokines and inflammatory parameters such as cyclooxygenase-2 (COX-2), inducible nitric oxidase synthase (iNOS) and prostaglandin (PGE2). Pterostilbene significantly (p < 0.001) down-regulated the level of metalloproteinases (MMP) such as MMP-2 and MMP-9. Pterostilbene suppressed the cellular swelling, cellular disintegration, macrophage infiltration, monocyte infiltration and polymorphonuclear leucocyte degranulation in the brain.Conclusion: In conclusion, Pterostilbene exhibited the neuroprotective effect against cerebral ischemia in rats via anti-inflammatory mechanism.

The evaluation of the catalytic capacity of catalysts is indispensable research, as catalytic capacity is a crucial factor to dictate the efficiency of heterogeneous Fenton catalysis. Herein, we obtained cigarette tar-methanol extracts (CTME) by applying methanol to cigarette tar and found that CTME could cause CL reactions with Fe2+/H2O2 systems in acidic, neutral, and alkaline media. The CL spectrum experiment indicated that the emission wavelengths of the CTME CL reaction with Fe2+/H2O2 systems were about 490 nm, 535 nm, and 590 nm. Quenching experiments confirmed that hydroxyl radicals (•OH) were responsible for the CL reaction for CTME. Then the CL property of CTME was applied in-situ to rapidly determine the amounts of •OH in tetrachloro-1,4-benzoquinone (TCBQ)/H2O2 system in acidic, neutral and alkaline media, and the CL intensities correlated the best (R2 = 0.99) with TCBQ concentrations. To demonstrate the utility of the CTME CL method, the catalytic capacity of different types and concentrations of catalysts in heterogeneous Fenton catalysis were examined. It was found that the order of CL intensities was consistent with the order of degradation efficiencies of Rhodamine B, indicating that this method could distinguish the catalytic capacity of catalysts. The CTME CL method could provide a convenient tool for the efficient evaluation of the catalytic capacity of catalysts in heterogeneous Fenton catalysis.

In this study, theoretical models were proposed to explain the changes in self-magnetic flux density (SMFD) due to fatigue cracks in the presence and absence of external magnetic fields. Three theoretical models were proposed: rotation domain model (RDM), concentration domain model (CDM), and vertical domain model (VDM), considering the deformation and non-deformation possibilities. To prove the theoretical model, fatigue cracks with different depth angles were fabricated through fatigue testing and EDM processing on the CT specimens. In addition, tunnel magnetoresistance (TMR) sensors were used to evaluate the 3-axis distribution of SMFD. Comparing the simulation and experimental results, similar tendencies of the occurrence and depth angle of fatigue cracks and their effect on the distribution of SMFD were observed. According to the RDM, the distribution of SMFD occurs in the direction of the crack length (y-direction), while the CDM explains that the SMFD does not occur if the fatigue crack is in a direction perpendicular to the surface. In addition, the VDM shows that SMFDs occur in a direction perpendicular to the crack length (x-direction) and the specimen surface (z-direction). Interestingly, these trends agree with the experimental results, which confirms the validity of the theoretical model and thus can be used to estimate the depth direction of a fatigue crack.

Carbon steels are commonly used in railroad, shipment, building, and bridge construction. They provide excellent ductility and toughness when exposed to external stresses. They are able to resist stresses and strains effectively, and guarantee safe operation of the devices through nondestructive testing (NDT). The magnetic metal memory (MMM) can be used as an NDT method to measure the residual stress. The ability of carbon steel to produce a magnetic memory effect under stress is explored here, and enables the magnetic flux density to be analyzed. The relationship between stress and magnetic flux density has not been fully presented until now. The purpose of this paper is to assess the relationship between stress distribution and the magnetic flux density measured by the experiment. For this, an experimental method for examining a carbon steel plate (SA 106), based on the four-point loading test, was used. The effect of stresses resulting from the applied loads on the response of the experimented SA 106 specimen was examined. A three directional tunnel magnetoresistance (TMR) measurement system was used to collect the triaxial magnetic flux density distribution in the SA 106 specimen. In addition, finite element method (FEM) analyses were performed, and provided information on the direction and distribution of the stress over the studied SA 106 specimen. Indeed, a correlation was derived by comparing the stress analysis by FEM and the measured triaxial magnetic flux density.

Carbon steel pipe is used in various industries, including nuclear power plants. Due to the daily cyclic operation of the pipe over time, environmental influences, and extreme working conditions, the probability of developing small fine cracks in the welded areas of the pipes increases. For that reason, it requires earlier assessment, and providing adequate inspection and evaluation of the weld area of the pipes used in such an installation is crucial to increase the safety level. In this paper, two different probe configurations were used to assess the integrity of the girth weld of the SA106 carbon steel pipe welded by gas tungsten arc welding. The conventional eddy current probe was initially used, but as it had some limitations, a new probe configuration was proposed to overcome these constraints. Numerical simulations using the finite element method were performed, based on the real measurement of the physical properties of the specimen, to complement the experimental data. In addition, the experimental results were successfully reproduced by the simulations. Simulation and experimental results show that the proposed probe configuration allows adequate inspection.

Three-dimensional observation of metal grains (MG) has a wide potential application serving the interdisciplinary community. It can be used for industrial applications and basic research to overcome the limitations of non-destructive testing methods, such as ultrasonic testing, magnetic particle testing, and eddy current testing. This study proposes a method and its implementation algorithm to observe (MG) metal grains in three dimensions in a general laboratory environment equipped with a polishing machine and a metal microscope. An image was taken by a metal microscope while polishing the mounted object to be measured. Then, the metal grains (MGs) were reconstructed into three dimensions through local positioning, binarization, boundary extraction, (MG) selection, and stacking. The goal is to reconstruct the 3D MG in a virtual form that reflects the real shape of the MG. The usefulness of the proposed method was verified using the carbon steel (SA106) specimen.

By using minimax methods in critical point theory, we obtain the existence of periodic solutions for second-order ordinary differential equations with linear nonlinearity.

In the title compound, [Mn(C10H7N2O2)2(H2O)4], the MnII atom, lying on an inversion center, has an octahedral environment with four coordinated water molecules in the equatorial plane and two N atoms from two 4-(imidazol-1-yl)benzoate ligands at the axial sites. The complex molecules are connected into a three-dimensional network by extensive hydrogen bonds between the water molecules and the carboxylate O atoms.

Background. Wound healing is an essential physiological process in recovery after microsurgery. Objectives. To further understand the functions of fibroblast growth factor 21 (FGF21), the roles of this factor were examined and its correlations with inflammation, vascular endothelial growth factor A (VEGFA) and ERK1/2 signaling pathway activation were analyzed. Materials and methods. Rat brain microvascular endothelial cells (RBMECs) were treated with interleukin (IL)-1ß and used for the experiments. Cell Counting Kit-8 (CCK-8) was used to detect the cell viability of RBMECs after treatment with IL-1ß (1 ng/mL) and FGF21 or VEGFA overexpression, while changes in apoptosis were measured through flow cytometry. Migration was checked through the scratch test. FGF21 and VEGFA RNA expression was assessed using reverse-transcription quantitative polymerase chain reaction (RT-qPCR), which was also used to examine RNA expression of Bcl-2, Bax and caspase-3. After IL-1ß treatment and FGF21 overexpression, tumor necrosis factor alpha (TNF-a) and tumor growth factor ß1 (TGF-ß1) proteins level were observed with enzyme-linked immunosorbent assay (ELISA), which was also applied to check the expression of ERK1/2 after overexpression of FGF21 and VEGFA. PD98059 (50 µM), an ERK1/2 inhibitor, was used to examine the roles of ERK1/2 in regulating cell viability and apoptosis. Results. The IL-1ß treatment significantly decreased the viability of RBMECs and TGF-ß1, but promoted cell apoptosis and TNF-a expression. FGF21 was downregulated by IL-1ß treatment but its overexpression enhanced the viability of RBMECs and TGF-ß1 and ERK1/2 protein levels, and attenuated cell apoptosis and TNF-a. Upregulated TNF-a restrained cell viability and apoptosis of RBMECs after FGF21 overexpression, and its upregulation not only suppressed FGF21, but also VEGFA. Moreover, VEGFA suppression by TNF-a increased cell viability and ERK1/2 protein levels, and suppressed the apoptosis of RBMECs through its upregulation. However, PD98059 obstructed the functions of FGF21 and VEGFA. Conclusions. FGF21 promoted the cell viability of RBMECs through upregulating TNF-a-mediated VEGFA and ERK1/2 signaling. [ABSTRACT FROM AUTHOR]

The inhibitory effect of matrine (MA) was studied in combination with irinotecan (CPT-11) on proliferation of human colon carcinoma cell line HT29. We also explored the mechanism of cell apoptosis induction in HT29. HT29 cells were treated with different concentrations of MA and CPT-11 alone and in combination. The growth inhibition in HT29 cells was evaluated using MTT assay. Apoptosis was detected using AV-PI double staining flow cytometry. Transmission electron microscopy was used to detect structural changes in cells. Topoisomerase (TOPO) I, Bax and Caspase-3 expression levels were evaluated using western blot analysis. MA and CPT-11 alone and in combination, inhibited the proliferation of HT29 cells, whereas the combination treatment exhibited higher inhibitory effect (P<0.01). This suggests the existence of synergistic cytotoxicity. Compared with each treatment alone, the combination treatment caused more significant damage to cell structure, and caused a significantly higher apoptosis rate (P<0.01). Additionally, the combination treatment increased TOPO I, Bax and Caspase-3 expression levels (P<0.01). In conclusion, MA in combination with CPT-11 synergistically inhibited HT29 cell proliferation and induced apoptosis in these cells. The mechanism may be related to upregulation of the TOPO I, Bax and Caspase-3 protein expression. [ABSTRACT FROM AUTHOR]

Reactive oxygen species (ROS) play very important roles in the photocatalytic reactions of semiconductors. Using a continuous flow chemiluminescence (CFCL) system, we developed three methods for the selective, sensitive, and online detection of O2•-, •OH, and H2O2 generated during ultraviolet (UV) irradiation of nano-TiO2 suspensions. TiO2 nanoparticles were irradiated in a photoreactor and pumped continuously into a detection cell. To detect O2•-, luminol was mixed with TiO2 before it entered the detection cell. For the detection of shortlived •OH, phthalhydrazide was added into the photoreactor to capture •OH, and then mixed with H2O2/KsCu(HIO6)2 to produce chemiluminescence (CL). To detect H2O2, an irradiated TiO2 suspension was kept in darkness for 30 min, and then mixed with luminol/K3Fe(CN)6 to produce CL. The selectivity of each method for a particular ROS was verified by using specific ROS scavengers. For a given ROS, a comparison between CL and conventional method showed good agreement for a series of TiO2 concentrations. The sensitivity of CL method was approximately 3-, 1200-, and 5-fold higher than the conventional method for O2•-, •OH, and H2O2 respectively. To demonstrate the utility of the methods, ROS in three different types of TiO2 suspensions was detected by CFCL. It was found that photodegradation efficiency of Rhodamine B correlated the best (R2 > 0.95) with the amount of photogenerated •OH, implying that •OH was the major oxidant in Rhodamine B photodegradation reaction. CFCL may provide a convenient tool for the studies on the reaction kinetics of ROS-partidpated decomposition of environmental contaminants. [ABSTRACT FROM AUTHOR]

Extracellular matrix metalloproteinase inducer (EMMPRIN), a member of the immunoglobulin superfamily, is present on the surface of tumor cells where it stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPs). We have analyzed the clinicopathological characteristics of EMMPRIN and MMP2 expression in normal brain tissue and pediatric gliomas and evaluated their prognostic value in diagnosing the latter. Immunochemistry analysis revealed EMMPRIN and MMP2 expression in cryo-sections of pediatric gliomas (45 samples) and normal brain tissue (20 samples). Both EMMPRIN and MMP2 were expressed in normal brain and glioma tissues with different levels of malignancy. The intensively positive expression rates of EMMPRIN (22/27) and MMP2 (21/27) in anaplastic astrocytoma and glioblastoma tissues were significantly higher than those in normal brain and low-grade astrocytoma tissues (2/28 and (1/2)8, respectively). Spearman analysis indicated that the expression level of EMMPRIN was significantly positively correlated with that of MMP2 (r = 0.86, p < 0.01). The positive expression of EMMPRIN and MMP2 was associated with higher grade gliomas. Patients with EMMPRIN+/MMP2+ expression had the lowest survival rate (p < 0.01). Based on these results, we conclude that EMMPRIN and MMP2 are expressed differently in normal brain and pediatric gliomas. The detection of their co-expression may facilitate the prediction of pediatric gliomas prognosis. [ABSTRACT FROM AUTHOR]