46 results on '"DeNicola, Gina"'
Search Results
2. Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC
- Author
-
Ward, Nathan P., Yoon, Sang Jun, Flynn, Tyce, Sherwood, Amanda M., Olley, Maddison A., Madej, Juliana, and DeNicola, Gina M.
- Published
- 2024
- Full Text
- View/download PDF
3. Ferroptosis in health and disease
- Author
-
Berndt, Carsten, Alborzinia, Hamed, Amen, Vera Skafar, Ayton, Scott, Barayeu, Uladzimir, Bartelt, Alexander, Bayir, Hülya, Bebber, Christina M., Birsoy, Kivanc, Böttcher, Jan P., Brabletz, Simone, Brabletz, Thomas, Brown, Ashley R., Brüne, Bernhard, Bulli, Giorgia, Bruneau, Alix, Chen, Quan, DeNicola, Gina M., Dick, Tobias P., Distéfano, Ayelén, Dixon, Scott J., Engler, Jan B., Esser-von Bieren, Julia, Fedorova, Maria, Friedmann Angeli, José Pedro, Friese, Manuel A., Fuhrmann, Dominic C., García-Sáez, Ana J., Garbowicz, Karolina, Götz, Magdalena, Gu, Wei, Hammerich, Linda, Hassannia, Behrouz, Jiang, Xuejun, Jeridi, Aicha, Kang, Yun Pyo, Kagan, Valerian E., Konrad, David B., Kotschi, Stefan, Lei, Peng, Le Tertre, Marlène, Lev, Sima, Liang, Deguang, Linkermann, Andreas, Lohr, Carolin, Lorenz, Svenja, Luedde, Tom, Methner, Axel, Michalke, Bernhard, Milton, Anna V., Min, Junxia, Mishima, Eikan, Müller, Sebastian, Motohashi, Hozumi, Muckenthaler, Martina U., Murakami, Shohei, Olzmann, James A., Pagnussat, Gabriela, Pan, Zijan, Papagiannakopoulos, Thales, Pedrera Puentes, Lohans, Pratt, Derek A., Proneth, Bettina, Ramsauer, Lukas, Rodriguez, Raphael, Saito, Yoshiro, Schmidt, Felix, Schmitt, Carina, Schulze, Almut, Schwab, Annemarie, Schwantes, Anna, Soula, Mariluz, Spitzlberger, Benedikt, Stockwell, Brent R., Thewes, Leonie, Thorn-Seshold, Oliver, Toyokuni, Shinya, Tonnus, Wulf, Trumpp, Andreas, Vandenabeele, Peter, Vanden Berghe, Tom, Venkataramani, Vivek, Vogel, Felix C.E., von Karstedt, Silvia, Wang, Fudi, Westermann, Frank, Wientjens, Chantal, Wilhelm, Christoph, Wölk, Michele, Wu, Katherine, Yang, Xin, Yu, Fan, Zou, Yilong, and Conrad, Marcus
- Published
- 2024
- Full Text
- View/download PDF
4. ASCT2 is a major contributor to serine uptake in cancer cells
- Author
-
Conger, Kelly O., Chidley, Christopher, Ozgurses, Mete Emir, Zhao, Huiping, Kim, Yumi, Semina, Svetlana E., Burns, Philippa, Rawat, Vipin, Lietuvninkas, Lina, Sheldon, Ryan, Ben-Sahra, Issam, Frasor, Jonna, Sorger, Peter K., DeNicola, Gina M., and Coloff, Jonathan L.
- Published
- 2024
- Full Text
- View/download PDF
5. NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis
- Author
-
Ilter, Didem, Drapela, Stanislav, Schild, Tanya, Ward, Nathan P., Adhikari, Emma, Low, Vivien, Asara, John, Oskarsson, Thordur, Lau, Eric K., DeNicola, Gina M., McReynolds, Melanie R., and Gomes, Ana P.
- Published
- 2023
- Full Text
- View/download PDF
6. Source of nicotinamide governs its metabolic fate in cultured cells, mice, and humans
- Author
-
Dutta, Tumpa, Kapoor, Nidhi, Mathew, Meril, Chakraborty, Suban S., Ward, Nathan P., Prieto-Farigua, Nicolas, Falzone, Aimee, DeLany, James P., Smith, Steven R., Coen, Paul M., DeNicola, Gina M., and Gardell, Stephen J.
- Published
- 2023
- Full Text
- View/download PDF
7. A tandem activity-based sensing and labeling strategy reveals antioxidant response element regulation of labile iron pools.
- Author
-
Pezacki, Aidan T., Gonciarz, Ryan L., Toshitaka Okamura, Matier, Carson D., Torrente, Laura, Ke Cheng, Miller, Sophia G., Ralle, Martina, Ward, Nathan P., DeNicola, Gina M., Renslo, Adam R., and Chang, Christopher J.
- Subjects
IRON ,TRANSITION metals ,IRON chelates ,FLUORESCENCE quenching ,IRON proteins ,CURCUMIN - Abstract
Iron is an essential element for life owing to its ability to participate in a diverse array of oxidation-reduction reactions. However, misregulation of iron-dependent redox cycling can also produce oxidative stress, contributing to cell growth, proliferation, and death pathways underlying aging, cancer, neurodegeneration, and metabolic diseases. Fluorescent probes that selectively monitor loosely bound Fe(II) ions, termed the labile iron pool, are potentially powerful tools for studies of this metal nutrient; however, the dynamic spatiotemporal nature and potent fluorescence quenching capacity of these bioavailable metal stores pose challenges for their detection. Here, we report a tandem activity-based sensing and labeling strategy that enables imaging of labile iron pools in live cells through enhancement in cellular retention. Iron green-1 fluoromethyl (IG1-FM) reacts selectively with Fe(II) using an endoperoxide trigger to release a quinone methide dye for subsequent attachment to proximal biological nucleophiles, providing a permanent fluorescent stain at sites of elevated labile iron. IG1-FM imaging reveals that degradation of the major iron storage protein ferritin through ferritinophagy expands the labile iron pool, while activation of nuclear factor-erythroid 2-related factor 2 (NRF2) antioxidant response elements (AREs) depletes it. We further show that lung cancer cells with heightened NRF2 activation, and thus lower basal labile iron, have reduced viability when treated with an iron chelator. By connecting labile iron pools and NRF2-ARE activity to a druggable metal-dependent vulnerability in cancer, this work provides a starting point for broader investigations into the roles of transition metal and antioxidant signaling pathways in health and disease. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. Establishing a living biobank of patient-derived organoids of intraductal papillary mucinous neoplasms of the pancreas
- Author
-
Beato, Francisca, Reverón, Dayana, Dezsi, Kaleena B., Ortiz, Antonio, Johnson, Joseph O., Chen, Dung-Tsa, Ali, Karla, Yoder, Sean J., Jeong, Daniel, Malafa, Mokenge, Hodul, Pamela, Jiang, Kun, Centeno, Barbara A., Abdalah, Mahmoud A., Balasi, Jodi A., Tassielli, Alexandra F., Sarcar, Bhaswati, Teer, Jamie K., DeNicola, Gina M., Permuth, Jennifer B., and Fleming, Jason B.
- Published
- 2021
- Full Text
- View/download PDF
9. Non-canonical Glutamate-Cysteine Ligase Activity Protects against Ferroptosis
- Author
-
Kang, Yun Pyo, Mockabee-Macias, Andrea, Jiang, Chang, Falzone, Aimee, Prieto-Farigua, Nicolas, Stone, Everett, Harris, Isaac S., and DeNicola, Gina M.
- Published
- 2021
- Full Text
- View/download PDF
10. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion
- Author
-
Harris, Isaac S., Endress, Jennifer E., Coloff, Jonathan L., Selfors, Laura M., McBrayer, Samuel K., Rosenbluth, Jennifer M., Takahashi, Nobuaki, Dhakal, Sabin, Koduri, Vidyasagar, Oser, Matthew G., Schauer, Nathan J., Doherty, Laura M., Hong, Andrew L., Kang, Yun Pyo, Younger, Scott T., Doench, John G., Hahn, William C., Buhrlage, Sara J., DeNicola, Gina M., Kaelin, William G., Jr., and Brugge, Joan S.
- Published
- 2019
- Full Text
- View/download PDF
11. PHGDH supports liver ceramide synthesis and sustains lipid homeostasis
- Author
-
Kang, Yun Pyo, Falzone, Aimee, Liu, Min, González-Sánchez, Paloma, Choi, Bo-Hyun, Coloff, Jonathan L., Saller, James J., Karreth, Florian A., and DeNicola, Gina M.
- Published
- 2020
- Full Text
- View/download PDF
12. A PI3K gene expression signature predicts for recurrence in early‐stage non–small cell lung cancer treated with stereotactic body radiation therapy.
- Author
-
Sebastian, Nikhil T., Webb, Amy, Shilo, Konstantin, Robb, Ryan, Xu‐Welliver, Meng, Haglund, Karl, Brownstein, Jeremy, DeNicola, Gina M., Shen, Changxian, and Williams, Terence M.
- Subjects
STEREOTACTIC radiotherapy ,NON-small-cell lung carcinoma ,CANCER relapse ,PHOSPHATIDYLINOSITOL 3-kinases ,GENE expression ,DISEASE risk factors - Abstract
Introduction: Increasingly, early‐stage non–small cell lung cancer (NSCLC) is treated with stereotactic body radiation therapy (SBRT). Although treatment is generally effective, a small subset of tumors will recur because of radioresistance. Preclinical studies suggested PI3K‐AKT‐mTOR activation mediates radioresistance. This study sought to validate this finding in tumor samples from patients who underwent SBRT for NSCLC. Methods: Patients with T1‐3N0 NSCLC treated with SBRT at our institution were included. Total RNA of formalin‐fixed paraffin‐embedded tumor biopsy specimens (pretherapy) was isolated and analyzed using the Clariom D assay. Risk scores from a PI3K activity signature and four published NSCLC signatures were generated and dichotomized by the median. Kaplan–Meier curves and Cox regressions were used to analyze their association with recurrence and overall survival (OS). The PI3K signature was also tested in a data set of resected NSCLC for additional validation. Results: A total of 92 patients were included, with a median follow‐up of 18.3 months for living patients. There was no association of any of the four published gene expression signatures with recurrence or OS. However, high PI3K risk score was associated with higher local recurrence (hazard ratio [HR], 11.72; 95% CI, 1.40–98.0; p =.023) and worse disease‐free survival (DFS) (HR, 3.98; 95% CI, 1.57–10.09; p =.0035), but not OS (p =.49), regional recurrence (p =.15), or distant recurrence (p =.85). In the resected NSCLC data set (n = 361), high PI3K risk score was associated with decreased OS (log‐rank p =.013) but not DFS (p = 0.54). Conclusions: This study validates that higher PI3K activity, measured by gene expression, is associated with local recurrence and worse DFS in early‐stage NSCLC patients treated with SBRT. This may be useful in prognostication and/or tailoring treatment, and merits further validation. In this single‐institution study of 92 patients with T1‐3 non–small cell lung cancer treated with stereotactic body radiation therapy, it was found that high PI3K activity, measured by gene expression using pretreatment biopsy specimens, was associated with worse local recurrence and disease‐free survival. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
13. Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers
- Author
-
Mullarky, Edouard, Lucki, Natasha C., Zavareh, Reza Beheshti, Anglin, Justin L., Gomes, Ana P., Nicolay, Brandon N., Wong, Jenny C. Y., Christen, Stefan, Takahashi, Hidenori, Singh, Pradeep K., Blenis, John, Warren, J. David, Fendt, Sarah-Maria, Asara, John M., DeNicola, Gina M., Lyssiotis, Costas A., Lairson, Luke L., and Cantley, Lewis C.
- Published
- 2016
14. Proteogenomic landscape of squamous cell lung cancer
- Author
-
Stewart, Paul A., Welsh, Eric A., Slebos, Robbert J. C., Fang, Bin, Izumi, Victoria, Chambers, Matthew, Zhang, Guolin, Cen, Ling, Pettersson, Fredrik, Zhang, Yonghong, Chen, Zhihua, Cheng, Chia-Ho, Thapa, Ram, Thompson, Zachary, Fellows, Katherine M., Francis, Jewel M., Saller, James J., Mesa, Tania, Zhang, Chaomei, Yoder, Sean, DeNicola, Gina M., Beg, Amer A., Boyle, Theresa A., Teer, Jamie K., Ann Chen, Yian, Koomen, John M., Eschrich, Steven A., and Haura, Eric B.
- Published
- 2019
- Full Text
- View/download PDF
15. NOX4-dependent fatty acid oxidation promotes NLRP3 inflammasome activation in macrophages
- Author
-
Moon, Jong-Seok, Nakahira, Kiichi, Chung, Kuei-Pin, DeNicola, Gina M, Koo, Michael Jakun, Pabón, Maria A, Rooney, Kristen T, Yoon, Joo-Heon, Ryter, Stefan W, Stout-Delgado, Heather, and Choi, Augustine M K
- Subjects
Oxidases -- Properties ,Inflammasomes -- Genetic aspects ,Enzyme activation -- Health aspects ,Transferases -- Properties ,Cellular signal transduction -- Genetic aspects -- Health aspects ,Biological sciences ,Health - Abstract
Altered metabolism has been implicated in the pathogenesis of inflammatory diseases. NADPH oxidase 4 (NOX4), a source of cellular superoxide anions, has multiple biological functions that may be of importance in inflammation and in the pathogenesis of human metabolic diseases, including diabetes. However, the mechanisms by which NOX4-:dependent metabolic regulation affect the innate immune response remain unclear. Here we show that deficiency of NOX4 resulted in reduced expression of carnitine palmitoyltransferase 1A (CPT1A), which is a key mitochondrial enzyme in the fatty acid oxidation (FAO) pathway. The reduced FAO resulted in less activation of the nucleotide-binding domain, leucine-:rich-repeat-:containing receptor (NLR), pyrin-domain-:containing 3 (NLRP3) inflammasome in human and mouse macrophages. In contrast, NOX4 deficiency did not inhibit the activation of the NLR family, CARD-domain-containing 4 (NLRC4), the NLRP1 or the absent in melanoma 2 (AIM2) inflammasomes. We also found that inhibition of FAO by etomoxir treatment suppressed NLRP3 inflammasome activation. Furthermore, Nox4-deficient mice showed substantial reduction in caspase-1 activation and in interleukin (IL)-1[beta] and IL-18 production, and there was improved survival in a mouse model of NLRP3-:mediated Streptococcus pneumoniae infection. The pharmacologic inhibition of NOX4 by either GKT137831, which is currently in phase 2 clinical trials, or VAS-2870 attenuated NLRP3 inflammasome activation. Our results suggest that NOX4-mediated FAO promotes NLRP3 inflammasome activation., Author(s): Jong-Seok Moon [1, 2]; Kiichi Nakahira [1, 2]; Kuei-Pin Chung [1, 2, 3]; Gina M DeNicola [1]; Michael Jakun Koo [1, 2]; Maria A Pabón [1]; Kristen T Rooney [...]
- Published
- 2016
- Full Text
- View/download PDF
16. RETRACTED ARTICLE: NOX4-dependent fatty acid oxidation promotes NLRP3 inflammasome activation in macrophages
- Author
-
Moon, Jong-Seok, Nakahira, Kiichi, Chung, Kuei-Pin, DeNicola, Gina M, Koo, Michael Jakun, Pabón, Maria A, Rooney, Kristen T, Yoon, Joo-Heon, Ryter, Stefan W, Stout-Delgado, Heather, and Choi, Augustine M K
- Published
- 2016
- Full Text
- View/download PDF
17. Inhibition of Hedgehog Signaling Enhances Delivery of Chemotherapy in a Mouse Model of Pancreatic Cancer
- Author
-
Olive, Kenneth P., Jacobetz, Michael A., Davidson, Christian J., Gopinathan, Aarthi, McIntyre, Dominick, Honess, Davina, Madhu, Basetti, Goldgraben, Mae A., Caldwell, Meredith E., Allard, David, Frese, Kristopher K., DeNicola, Gina, Feig, Christine, Combs, Chelsea, Winter, Stephen P., Ireland-Zecchini, Heather, Reichelt, Stefanie, Howat, William J., Chang, Alex, Dhara, Mousumi, Wang, Lifu, Rückert, Felix, Grützmann, Robert, Pilarsky, Christian, Izeradjene, Kamel, Hingorani, Sunil R., Huang, Pearl, Davies, Susan E., Plunkett, William, Egorin, Merrill, Hruban, Ralph H., Whitebread, Nigel, McGovern, Karen, Adams, Julian, Iacobuzio-Donahue, Christine, Griffiths, John, and Tuveson, David A.
- Published
- 2009
- Full Text
- View/download PDF
18. Oxidation state-specific fluorescent copper sensors reveal oncogene-driven redox changes that regulate labile copper(II) pools.
- Author
-
Pezacki, Aidan T., Matier, Carson D., Xingxing Gu, Kummelstedt, Eric, Bond, Sarah E., Torrente, Laura, Jordan-Sciutto, Kelly L., DeNicola, Gina M., Su, Timothy A., Brady, Donita C., and Chang, Christopher J.
- Subjects
COPPER ,LEWIS acidity ,FLUORESCENCE quenching ,OXIDATION-reduction reaction ,FLUORESCENT probes - Abstract
Copper is an essential metal nutrient for life that often relies on redox cycling between Cu(I) and Cu(II) oxidation states to fulfill its physiological roles, but alterations in cellular redox status can lead to imbalances in copper homeostasis that contribute to cancer and other metalloplasias with metal-dependent disease vulnerabilities. Copper-responsive fluorescent probes offer powerful tools to study labile copper pools, but most of these reagents target Cu(I), with limited methods for monitoring Cu(II) owing to its potent fluorescence quenching properties. Here, we report an activity-based sensing strategy for turn-on, oxidation state-specific detection of Cu(II) through metal-directed acyl imidazole chemistry. Cu(II) binding to a metal and oxidation state-specific receptor that accommodates the harder Lewis acidity of Cu(II) relative to Cu(I) activates the pendant dye for reaction with proximal biological nucleophiles and concomitant metal ion release, thus avoiding fluorescence quenching. Copper-directed acyl imidazole 649 for Cu(II) (CD649.2) provides foundational information on the existence and regulation of labile Cu(II) pools, including identifying divalent metal transporter 1 (DMT1) as a Cu(II) importer, labile Cu(II) increases in response to oxidative stress induced by depleting total glutathione levels, and reciprocal increases in labile Cu(II) accompanied by decreases in labile Cu(I) induced by oncogenic mutations that promote oxidative stress. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
19. Oncogene-induced Nrf2 transcription promotes ROS detoxification and tumorigenesis
- Author
-
DeNicola, Gina M., Karreth, Florian A., Humpton, Timothy J., Gopinathan, Aarthi, Wei, Cong, Frese, Kristopher, Mangal, Dipti, Yu, Kenneth H., Yeo, Charles J., Calhoun, Eric S., Scrimieri, Francesca, Winter, Jordan M., Hruban, Ralph H., Iacobuzio-Donahue, Christine, Kern, Scott E., Blair, Ian A., and Tuveson, David A.
- Subjects
Gene expression -- Research ,Oncogenes -- Physiological aspects -- Research ,Polymerase chain reaction -- Usage ,Reactive oxygen species -- Physiological aspects -- Research ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Reactive oxygen species (ROS) are mutagenic and may thereby promote cancer (1). Normally, ROS levels are tightly controlled by an inducible antioxidant program that responds to cellular stressors and is predominantly regulated by the transcription factor Nrf2 (also known as Nfe2l2) and its repressor protein Keapl (refs 2-5). In contrast to the acute physiological regulation of Nrf2, in neoplasia there is evidence for increased basal activation of Nrf2. Indeed, somatic mutations that disrupt the Nrf2-Keap1 interaction to stabilize Nrf2 and increase the constitutive transcription of Nrf2 target genes were recently identified, indicating that enhanced ROS detoxification and additional Nrf2 functions may in fact be protumorigenic (6). Here, we investigated ROS metabolism in primary murine cells following the expression of endogenous oncogenic alleles of Kras, Braf and Myc, and found that ROS are actively suppressed by these oncogenes. [K-Ras.sup.G12D], [B-Raf.sup.V619E] and [Myc.sup.ERT2] each increased the transcription of Nrf2 to stably elevate the basal Nrf2 antioxidant program and thereby lower intracellular ROS and confer a more reduced intracellular environment. Oncogene-directed increased expression of Nrf2 is a new mechanism for the activation of the Nrf2 antioxidant program, and is evident in primary cells and tissues of mice expressing [K-Ras.sup.G12D] and [B-Raf.sup.V619E], and in human pancreatic cancer. Furthermore, genetic targeting of the Nrf2 pathway impairs [K-Ras.sup.G12D]-induced proliferation and tumorigenesis in vivo. Thus, the Nrf2 antioxidant and cellular detoxification program represents a previously unappreciated mediator of oncogenesis., To examine the role of ROS in cellular transformation and tumorigenesis, we used an endogenous and conditional oncogenic LSL-[K-Ras.sup.G12D] allele (7). [K-Ras.sup.G12D/+] and [K-Ras.sup.LSL/+] mouse embryonic fibroblasts (MEFs) were compared [...]
- Published
- 2011
- Full Text
- View/download PDF
20. Targeting NRF2 and Its Downstream Processes: Opportunities and Challenges.
- Author
-
Torrente, Laura and DeNicola, Gina M.
- Subjects
- *
DISEASE progression , *HOMEOSTASIS , *SURVIVAL , *CARCINOGENS , *CELL physiology , *CELLULAR signal transduction , *CHEMORADIOTHERAPY , *CELL proliferation , *TRANSCRIPTION factors , *CELL lines , *TUMORS , *DRUG resistance in cancer cells ,TUMOR prevention - Abstract
The transcription factor NRF2 coordinates the expression of a vast array of cytoprotective and metabolic genes in response to various stress inputs to restore cellular homeostasis. Transient activation of NRF2 in healthy tissues has been long recognized as a cellular defense mechanism and is critical to prevent cancer initiation by carcinogens. However, cancer cells frequently hijack the protective capability of NRF2 to sustain the redox balance and meet their metabolic requirements for proliferation. Further, aberrant activation of NRF2 in cancer cells confers resistance to commonly used chemotherapeutic agents and radiotherapy. During the last decade, many research groups have attempted to block NRF2 activity in tumors to counteract the survival and proliferative advantage of cancer cells and reverse resistance to treatment. In this review, we highlight the role of NRF2 in cancer progression and discuss the past and current approaches to disable NRF2 signaling in tumors. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
21. Genetic tools for the stable overexpression of circular RNAs.
- Author
-
Mecozzi, Nicol, Nenci, Arianna, Vera, Olga, Bok, Ilah, Falzone, Aimee, DeNicola, Gina M., and Karreth, Florian A.
- Subjects
CIRCULAR RNA ,GENETIC overexpression ,GENE expression ,RNA splicing ,NON-coding RNA ,LABORATORY mice - Abstract
Circular RNAs (circRNAs) are a class of non-coding RNAs featuring a covalently closed ring structure formed through backsplicing. circRNAs are broadly expressed and contribute to biological processes through a variety of functions. Standard gain-of-function and loss-of-function approaches to study gene functions have significant limitations when studying circRNAs. Overexpression studies in particular suffer from the lack of efficient genetic tools. While mammalian expression plasmids enable transient circRNA overexpression in cultured cells, most cell biological studies require long-term ectopic expression. Here we report the development and characterization of genetic tools enabling stable circRNA overexpression in vitro and in vivo. We demonstrated that circRNA expression constructs can be delivered to cultured cells via transposons, whereas lentiviral vectors have limited utility for the delivery of circRNA constructs due to viral RNA splicing in virus-producing cells. We further demonstrated ectopic circRNA expression in a hepatocellular carcinoma mouse model upon circRNA transposon delivery via hydrodynamic tail vein injection. Furthermore, we generated genetically engineered mice harbouring circRNA expression constructs. We demonstrated that this approach enables constitutive, global circRNA overexpression as well as inducible circRNA expression directed specifically to melanocytes in a melanoma mouse model. These tools expand the genetic toolkit available for the functional characterization of circRNAs. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
22. Cathepsin B promotes the progression of pancreatic ductal adenocarcinoma in mice
- Author
-
Gopinathan, Aarthi, DeNicola, Gina M, Frese, Kristopher K, Cook, Natalie, Karreth, Florian A, Mayerle, Julia, Lerch, Markus M, Reinheckel, Thomas, and Tuveson, David A
- Published
- 2012
- Full Text
- View/download PDF
23. The microbiome(s) and cancer: know thy neighbor(s).
- Author
-
González-Sánchez, Paloma and DeNicola, Gina M.
- Subjects
HUMAN microbiota ,IMMUNOREGULATION ,GUT microbiome ,TUMOR microenvironment ,DNA damage - Abstract
The human microbiome is essential for the correct functioning of many host physiological processes, including metabolic regulation and immune responses. Increasing evidence indicates that the microbiome may also influence cancer development, progression, and the response to therapy. Although most studies have focused on the effect of the gut microbiome, many other organs such as the skin, vagina, and lungs harbor their own microbiomes that are different from the gut. Tumor development has been associated with dysbiosis not only in the gut but also in the tissue from which the tumor originated. Furthermore, the intratumoral microbiota has a distinct signature in each tumor type. Here, we review the mechanisms by which the organ-specific microbiome can contribute to carcinogenesis: release of toxins that cause DNA damage and barrier failure; alteration of immune responses to create a local inflammatory or immunosuppressive environment; and regulation of nutrient levels in the tumor microenvironment through metabolite production and consumption. Solving the puzzle of how the microbiome influences the carcinogenesis process and treatment response requires an understanding of the two ways the microbiome can interact with cancer cells and the tumor microenvironment: through systemic effects exerted by the gut microbiota and local effects of the intratumoral microbiota. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
24. The Complex Interplay between Antioxidants and ROS in Cancer.
- Author
-
Harris, Isaac S. and DeNicola, Gina M.
- Subjects
- *
TUMOR classification , *ANTIOXIDANTS , *GENETIC testing , *TRANSCRIPTION factors , *GENETIC models , *TUMOR growth - Abstract
Reactive oxygen species (ROS) play important roles in tissue homeostasis, cellular signaling, differentiation, and survival. In this review, we discuss the types of ROS, their impact on cellular processes, and their pro- and antitumorigenic effects. Further, we discuss recent advances in our understanding of both endogenous and exogenous antioxidants in tumorigenic processes. Finally, we discuss how aberrant activation of antioxidant programs by the transcription factor NFE2-related factor 2 (NRF2) influences tumorigenesis and metastasis, and where the current gaps in our knowledge remain. New tools allow in vivo measurements of ROS in tumors. Mouse modeling and genetic screening approaches have revealed novel complexities and redundancies in endogenous antioxidant systems. Exogenous antioxidants may promote cancer through complex mechanisms. Aberrant NRF2 activation has diverse, and sometimes contradictory, impacts on tumor growth and metastasis. Tissue of origin, tumor stage, and the microenvironment greatly influence the influence of ROS on cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
25. The Regulation of NRF2 by Nutrient-Responsive Signaling and Its Role in Anabolic Cancer Metabolism.
- Author
-
Lee, Sae Bom, Sellers, Brianna N., and DeNicola, Gina M.
- Published
- 2018
- Full Text
- View/download PDF
26. Targeting Glutathione Reductase in KEAP1/NRF2 Mutant NSCLC.
- Author
-
Jiang, Chang and DeNicola, Gina
- Subjects
- *
GLUTATHIONE reductase , *NON-small-cell lung carcinoma - Published
- 2022
- Full Text
- View/download PDF
27. Recent advances in cancer metabolism: a technological perspective.
- Author
-
Kang, Yun Pyo, Ward, Nathan P., and DeNicola, Gina M.
- Published
- 2018
- Full Text
- View/download PDF
28. Retraction Notice to: mTORC1-Induced HK1-Dependent Glycolysis Regulates NLRP3 Inflammasome Activation.
- Author
-
Moon, Jong-Seok, Hisata, Shu, Park, Mi-Ae, DeNicola, Gina M., Ryter, Stefan W., Nakahira, Kiichi, and Choi, Augustine M.K.
- Published
- 2023
- Full Text
- View/download PDF
29. Long-sought mediator of vitamin K recycling discovered.
- Author
-
Ward, Nathan P. and DeNicola, Gina M.
- Abstract
The identity of the enzyme that enables vitamin K to combat the adverse side effects of a drug called warfarin has been long sought. Analysis of a type of cell death called ferroptosis has now unexpectedly solved the mystery. The protein FSP1 inhibits ferroptotic cell death. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
30. A pilot study of radiologic measures of abdominal adiposity: weighty contributors to early pancreatic carcinogenesis worth evaluating?
- Author
-
Permuth, Jennifer B., Choi, Jung W., Dung-Tsa Chen, Kun Jiang, DeNicola, Gina, Jian-Nong Li, Coppola, Domenico, Centeno, Barbara A., Magliocco, Anthony, Balagurunathan, Yoganand, Merchant, Nipun, Trevino, Jose G., and Jeong, Daniel
- Subjects
ABDOMINAL adipose tissue ,PANCREATIC cancer ,PANCREATIC duct ,TUMORS - Abstract
Objective: Intra-abdominal fat is a risk factor for pancreatic cancer (PC), but little is known about its contribution to PC precursors known as intraductal papillary mucinous neoplasms (IPMNs). Our goal was to evaluate quantitative radiologic measures of abdominal/visceral obesity as possible diagnostic markers of IPMN severity/pathology. Methods: In a cohort of 34 surgically-resected, pathologically-confirmed IPMNs (17 benign; 17 malignant) with preoperative abdominal computed tomography (CT) images, we calculated body mass index (BMI) and four radiologic measures of obesity: total abdominal fat (TAF) area, visceral fat area (VFA), subcutaneous fat area (SFA), and visceral to subcutaneous fat ratio (V/S). Measures were compared between groups using Wilcoxon two-sample exact tests and other metrics. Results: Mean BMI for individuals with malignant IPMNs (28.9 kg/m
2 ) was higher than mean BMI for those with benign IPMNs (25.8 kg/m2 ) (P=0.045). Mean VFA was higher for patients with malignant IPMNs (199.3 cm2 ) compared to benign IPMNs (120.4 cm2 ), P=0.092. V/S was significantly higher (P=0.013) for patients with malignant versus benign IPMNs (1.25 vs. 0.69 cm2 ), especially among females. The accuracy, sensitivity, specificity, and positive and negative predictive value of V/S in predicting malignant IPMN pathology were 74%, 71%, 76%, 75%, and 72%, respectively. Conclusions: Preliminary findings suggest measures of visceral fat from routine medical images may help predict IPMN pathology, acting as potential noninvasive diagnostic adjuncts for management and targets for intervention that may be more biologically-relevant than BMI. Further investigation of gender-specific associations in larger, prospective IPMN cohorts is warranted to validate and expand upon these observations. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
31. Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers.
- Author
-
Wong, Jenny C. Y., DeNicola, Gina M., Cantley, Lewis C., Mullarky, Edouard, Gomes, Ana P., Blenis, John, Singh, Pradeep K., Warren, J. David, Fendt, Sarah-Maria, Lyssiotis, Costas A., Lucki, Natasha C., Zavareh, Reza Beheshti, Anglin, Justin L., Lairson, Luke L., Christen, Stefan, Nicolay, Brandon N., Hidenori Takahashi, and Asara, John M.
- Subjects
- *
CANCER cells , *NEOPLASTIC cell transformation , *SERINE , *OLIGOMERIZATION , *GENETIC mutation , *CANCER chemotherapy - Abstract
Cancer cells reprogram their metabolism to promote growth and proliferation. The genetic evidence pointing to the importance of the amino acid serine in tumorigenesis is striking. The gene encoding the enzyme 3-phosphoglycerate dehydrogenase (PHGDH), which catalyzes the first committed step of serine biosynthesis, is overexpressed in tumors and cancer cell lines via focal amplification and nuclear factor erythroid-2-related factor 2 (NRF2)-mediated up-regulation. PHGDH-overexpressing cells are exquisitely sensitive to genetic ablation of the pathway. Here, we report the discovery of a selective small molecule inhibitor of PHGDH, CBR-5884, identified by screening a library of 800,000 drug-like compounds. CBR-5884 inhibited de novo serine synthesis in cancer cells and was selectively toxic to cancer cell lines with high serine biosynthetic activity. Biochemical characterization of the inhibitor revealed that it was a noncompetitive inhibitor that showed a time-dependent onset of inhibition and disrupted the oligomerization state of PHGDH. The identification of a small molecule inhibitor of PHGDH not only enables thorough preclinical evaluation of PHGDH as a target in cancers, but also provides a tool with which to study serine metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
32. Cancer’s Fuel Choice: New Flavors for a Picky Eater.
- Author
-
DeNicola, Gina M. and Cantley, Lewis C.
- Subjects
- *
WARBURG Effect (Oncology) , *GLYCOLYSIS , *CANCER cells , *BIOENERGETICS , *BIOSYNTHESIS , *GENETIC mutation - Abstract
Otto Warburg discovered that cancer cells exhibit a high rate of glycolysis in the presence of ample oxygen, a process termed aerobic glycolysis, in 1924 ( Warburg et al., 1924 ). Since then we have significantly advanced our understanding of cancers’ fuel choice to meet their demands for energy and for the production of biosynthetic precursors. In this review, we will discuss the preferred nutrients of cancer cells and how they are utilized to satisfy their bioenergetic and biosynthetic needs. In addition, we will describe how cell intrinsic and extrinsic factors such as oncogene mutations, nutrient and oxygen availability, and other microenvironmental factors influence fuel choice. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
33. mTORC1-Induced HK1-Dependent Glycolysis Regulates NLRP3 Inflammasome Activation.
- Author
-
Moon, Jong-Seok, Hisata, Shu, Park, Mi-Ae, DeNicola, Gina M., Ryter, Stefan W., Nakahira, Kiichi, and Choi, Augustine M.K.
- Abstract
Summary The mammalian target of rapamycin complex 1 (mTORC1) regulates activation of immune cells and cellular energy metabolism. Although glycolysis has been linked to immune functions, the mechanisms by which glycolysis regulates NLRP3 inflammasome activation remain unclear. Here, we demonstrate that mTORC1-induced glycolysis provides an essential mechanism for NLRP3 inflammasome activation. Moreover, we demonstrate that hexokinase 1 (HK1)-dependent glycolysis, under the regulation of mTORC1, represents a critical metabolic pathway for NLRP3 inflammasome activation. Downregulation of glycolysis by inhibition of Raptor/mTORC1 or HK1 suppressed both pro-IL-1β maturation and caspase-1 activation in macrophages in response to LPS and ATP. These results suggest that upregulation of HK1-dependent glycolysis by mTORC1 regulates NLRP3 inflammasome activation. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
34. The androgen receptor fuels prostate cancer by regulating central metabolism and biosynthesis.
- Author
-
Massie, Charles E, Lynch, Andy, Ramos-Montoya, Antonio, Boren, Joan, Stark, Rory, Fazli, Ladan, Warren, Anne, Scott, Helen, Madhu, Basetti, Sharma, Naomi, Bon, Helene, Zecchini, Vinny, Smith, Donna-Michelle, DeNicola, Gina M, Mathews, Nik, Osborne, Michelle, Hadfield, James, MacArthur, Stewart, Adryan, Boris, and Lyons, Scott K
- Subjects
ANDROGENS ,HORMONE receptors ,PROSTATE cancer treatment ,METABOLIC regulation ,BIOSYNTHESIS ,CANCER cell growth ,TUMOR growth ,PROTEIN kinase CK2 - Abstract
The androgen receptor (AR) is a key regulator of prostate growth and the principal drug target for the treatment of prostate cancer. Previous studies have mapped AR targets and identified some candidates which may contribute to cancer progression, but did not characterize AR biology in an integrated manner. In this study, we took an interdisciplinary approach, integrating detailed genomic studies with metabolomic profiling and identify an anabolic transcriptional network involving AR as the core regulator. Restricting flux through anabolic pathways is an attractive approach to deprive tumours of the building blocks needed to sustain tumour growth. Therefore, we searched for targets of the AR that may contribute to these anabolic processes and could be amenable to therapeutic intervention by virtue of differential expression in prostate tumours. This highlighted calcium/calmodulin-dependent protein kinase kinase 2, which we show is overexpressed in prostate cancer and regulates cancer cell growth via its unexpected role as a hormone-dependent modulator of anabolic metabolism. In conclusion, it is possible to progress from transcriptional studies to a promising therapeutic target by taking an unbiased interdisciplinary approach. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
35. C-Raf Inhibits MAPK Activation and Transformation by B-RafV600E
- Author
-
Karreth, Florian A., DeNicola, Gina M., Winter, Stephen P., and Tuveson, David A.
- Subjects
- *
GENETIC mutation , *MITOGEN-activated protein kinases , *CELL transformation , *GENETIC code , *CELLULAR signal transduction , *CELL proliferation , *GENE expression , *CANCER cells - Abstract
Summary: Activating B-Raf mutations that deregulate the MAPK pathway commonly occur in cancer. Whether additional proteins modulate the enzymatic activity of oncogenic B-Raf is unknown. Here we show that the proto-oncogene C-Raf paradoxically inhibits B-RafV600E kinase activity through the formation of B-RafV600E–C-Raf complexes. Although all Raf family members associate with oncogenic B-Raf, this inhibitory effect is specific to C-Raf. Indeed, a B-RafV600E isoform with impaired ability to interact with C-Raf exhibits elevated oncogenic potential. Human melanoma cells expressing B-RafV600E display a reduced C-Raf:B-Raf ratio, and further suppression of C-Raf increases MAPK activation and proliferation. Conversely, ectopic C-Raf expression lowers ERK phosphorylation and proliferation. Moreover, both oncogenic Ras and Sorafenib stabilize B-RafV600E–C-Raf complexes, thereby impairing MAPK activation. This inhibitory function of C-Raf on B-RafV600E-mediated MAPK activation may explain the lack of co-occurrence of B-RafV600E and oncogenic Ras mutations, and influence the successful clinical development of small molecule inhibitors for B-RafV600E-driven cancers. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
36. RAS in cellular transformation and senescence
- Author
-
DeNicola, Gina M. and Tuveson, David A.
- Published
- 2009
- Full Text
- View/download PDF
37. NRF2 regulates serine biosynthesis in non-small cell lung cancer
- Author
-
DeNicola, Gina M., Chen, Pei-Hsuan, Mullarky, Edouard, Sudderth, Jessica A., Hu, Zeping, Wu, David, Tang, Hao, Xie, Yang, Asara, John M., Huffman, Kenneth E., Wistuba, Ignacio I., Minna, John D., DeBerardinis, Ralph J., and Cantley, Lewis C.
- Published
- 2015
- Full Text
- View/download PDF
38. Dissecting the Crosstalk between NRF2 Signaling and Metabolic Processes in Cancer.
- Author
-
DeBlasi, Janine M. and DeNicola, Gina M.
- Subjects
- *
AMINO acid metabolism , *LIPID metabolism , *AUTOPHAGY , *CELL proliferation , *REACTIVE oxygen species , *METABOLISM , *METABOLITES , *METASTASIS , *TRANSCRIPTION factors , *TUMORS , *OXIDATIVE stress , *CELL survival - Abstract
Simple Summary: The stress-responsive transcription factor NRF2 (nuclear factor-erythroid 2 p45-related factor 2) directs cellular metabolic processes that can have diverse effects in the context of cancer. This review addresses how NRF2 and its negative regulator KEAP1 (Kelch-like ECH-associated protein 1) collectively modulate and respond to metabolism. We highlight NRF2-regulated processes relevant to the antioxidant system, cellular proliferation, and survival, including metabolism of amino acids, lipids, NADPH (reduced nicotinamide adenine dinucleotide phosphate), iron, and heme. We also review the stabilization of NRF2 by electrophiles, metabolites, and autophagy. Finally, we discuss topics that warrant further investigation into the KEAP1/NRF2 pathway's role in tumor progression. The transcription factor NRF2 (nuclear factor-erythroid 2 p45-related factor 2 or NFE2L2) plays a critical role in response to cellular stress. Following an oxidative insult, NRF2 orchestrates an antioxidant program, leading to increased glutathione levels and decreased reactive oxygen species (ROS). Mounting evidence now implicates the ability of NRF2 to modulate metabolic processes, particularly those at the interface between antioxidant processes and cellular proliferation. Notably, NRF2 regulates the pentose phosphate pathway, NADPH production, glutaminolysis, lipid and amino acid metabolism, many of which are hijacked by cancer cells to promote proliferation and survival. Moreover, deregulation of metabolic processes in both normal and cancer-based physiology can stabilize NRF2. We will discuss how perturbation of metabolic pathways, including the tricarboxylic acid (TCA) cycle, glycolysis, and autophagy can lead to NRF2 stabilization, and how NRF2-regulated metabolism helps cells deal with these metabolic stresses. Finally, we will discuss how the negative regulator of NRF2, Kelch-like ECH-associated protein 1 (KEAP1), may play a role in metabolism through NRF2 transcription-independent mechanisms. Collectively, this review will address the interplay between the NRF2/KEAP1 complex and metabolic processes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
39. Dynamic ROS Control by TIGAR Regulates the Initiation and Progression of Pancreatic Cancer.
- Author
-
Cheung, Eric C., DeNicola, Gina M., Nixon, Colin, Blyth, Karen, Labuschagne, Christiaan F., Tuveson, David A., and Vousden, Karen H.
- Subjects
- *
PANCREATIC cancer , *CANCER invasiveness , *PRECANCEROUS conditions , *PANCREATIC tumors , *PANCREAS , *REACTIVE oxygen species , *SOLAR plexus , *CANCER cell migration - Abstract
The TIGAR protein has antioxidant activity that supports intestinal tissue repair and adenoma development. Using a pancreatic ductal adenocarcinoma (PDAC) model, we show that reactive oxygen species (ROS) regulation by TIGAR supports premalignant tumor initiation while restricting metastasis. Increased ROS in PDAC cells drives a phenotypic switch that increases migration, invasion, and metastatic capacity. This switch is dependent on increased activation of MAPK signaling and can be reverted by antioxidant treatment. In mouse and human, TIGAR expression is modulated during PDAC development, with higher TIGAR levels in premalignant lesions and lower TIGAR levels in metastasizing tumors. Our study indicates that temporal, dynamic control of ROS underpins full malignant progression and helps to rationalize conflicting reports of pro- and anti-tumor effects of antioxidant treatment. • ROS regulation by TIGAR supports premalignant pancreas tumor development • Increased ROS following TIGAR or Nrf2 loss enhance metastasis • ROS reduce DUSP6 expression to activate ERK and increase invasion and migration • TIGAR and ROS levels are dynamically regulated throughout tumor progression Cheung et al. show that TIGAR expression is dynamically regulated during the development of pancreatic ductal adenocarcinoma, resulting in lower levels of ROS to promote tumor initiation in the premalignant condition and higher levels of ROS that enable metastatic progression. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
40. The Non-Essential Amino Acid Cysteine Becomes Essential for Tumor Proliferation and Survival.
- Author
-
Combs, Joseph A. and DeNicola, Gina M.
- Subjects
- *
CYSTEINE metabolism , *CELL proliferation , *DRUG delivery systems , *GENE expression , *SURVIVAL , *ESSENTIAL amino acids ,TUMOR prognosis - Abstract
The non-essential amino acid cysteine is used within cells for multiple processes that rely on the chemistry of its thiol group. Under physiological conditions, many non-transformed tissues rely on glutathione, circulating cysteine, and the de novo cysteine synthesis (transsulfuration) pathway as sources of intracellular cysteine to support cellular processes. In contrast, many cancers require exogeneous cystine for proliferation and viability. Herein, we review how the cystine transporter, xCT, and exogenous cystine fuel cancer cell proliferation and the mechanisms that regulate xCT expression and activity. Further, we discuss the potential contribution of additional sources of cysteine to the cysteine pool and what is known about the essentiality of these processes in cancer cells. Finally, we discuss whether cyst(e)ine dependency and associated metabolic alterations represent therapeutically targetable metabolic vulnerabilities. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
41. On the illusion of auxotrophy: met1SΔ yeast cells can grow on inorganic sulfur, thanks to the previously uncharacterized homocysteine synthase Yll058w.
- Author
-
Van Oss, S. Branden, Parikh, Saurin Bipin, Coelho, Nelson Castilho, Wacholder, Aaron, Belashov, Ivan, Zdancewicz, Sara, Michaca, Manuel, Jiazhen Xu, Yun Pyo Kang, Ward, Nathan P., Sang Jun Yoon, McCourt, Katherine M., McKee, Jake, Ideker, Trey, VanDemark, Andrew P., DeNicola, Gina M., and Carvunis, Anne-Ruxandra
- Subjects
- *
HOMOCYSTEINE , *AUXOTROPHY , *SULFUR , *COMPLEMENTATION (Genetics) , *SULFUR metabolism - Abstract
Organisms must either synthesize or assimilate essential organic compounds to survive. The homocysteine synthase Met15 has been considered essential for inorganic sulfur assimilation in yeast since its discovery in the 1970s. As a result, MET15 has served as a genetic marker for hundreds of experiments that play a foundational role in eukaryote genetics and systems biology. Nevertheless, we demonstrate here through structural and evolutionary modeling, in vitro kinetic assays, and genetic complementation, that an alternative homocysteine synthase encoded by the previously uncharacterized gene YLL058W enables cells lacking Met15 to assimilate enough inorganic sulfur for survival and proliferation. These cells however fail to grow in patches or liquid cultures unless provided with exogenous methionine or other organosulfurs. We show that this growth failure, which has historically justified the status of MET15 as a classic auxotrophic marker, is largely explained by toxic accumulation of the gas hydrogen sulfide because of a metabolic bottleneck. When patched or cultured with a hydrogen sulfide chelator, and when propagated as colony grids, cells without Met15 assimilate inorganic sulfur and grow, and cells with Met15 achieve even higher yields. Thus, Met15 is not essential for inorganic sulfur assimilation in yeast. Instead, MET15 is the first example of a yeast gene whose loss conditionally prevents growth in a manner that depends on local gas exchange. Our results have broad implications for investigations of sulfur metabolism, including studies of stress response, methionine restriction, and aging. More generally, our findings illustrate how unappreciated experimental variables can obfuscate biological discovery. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
42. Fascin promotes lung cancer growth and metastasis by enhancing glycolysis and PFKFB3 expression.
- Author
-
Lin, Shengchen, Li, Yunzhan, Wang, Dezhen, Huang, Chongbiao, Marino, David, Bollt, Oana, Wu, Chaodong, Taylor, Matthew D., Li, Wei, DeNicola, Gina M., Hao, Jihui, Singh, Pankaj K., and Yang, Shengyu
- Subjects
- *
LUNG cancer , *GLYCOLYSIS , *TUMOR growth , *METASTASIS , *CANCER cell migration - Abstract
Fascin is a pro-metastatic actin-bundling protein that is upregulated in all metastatic carcinomas. Fascin promotes cancer cell migration and invasion by facilitating membrane protrusions, such as filopodia and invadopodia. Aerobic glycolysis is a key feature of cancer metabolism and provides critical intermediate metabolites for tumor growth. Here, we report that fascin increases glycolysis in lung cancer to promote tumor growth and metastasis. Fascin promotes glycolytic flux by increasing the expression and activities of phosphofructose-kinases 1 and 2 (PFK1 and 2). Fascin mediates glycolytic functions via activation of yes-associated protein 1 (YAP1) through its canonical actin-bundling activity by promoting the binding of YAP1 to a TEAD1/4 binding motif located 30 bp upstream of the PFKFB3 transcription start site to activate its transcription. Examination of the TCGA database suggests that the fascin-YAP1-PFKFB3 axis is likely conserved across different types of cancers. Importantly, pharmacological inhibitors of fascin suppressed YAP1-PFKFB3 signaling and glycolysis in cancer cell lines, organoid cultures, and xenograft metastasis models. Taken together, our data reveal that the glycolytic function of fascin is essential for the promotion of lung cancer growth and metabolism, and suggest that pharmacological inhibitors of fascin may be used to reprogram cancer metabolism in lung and potentially other cancers with fascin upregulation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
43. Mitochondrial Glutathione Catabolism Sustains Respiratory Function in Cysteine-Deprived NSCLC.
- Author
-
Ward, Nathan, Yoon, Sang Jun, Sherwood, Amanda, and DeNicola, Gina
- Subjects
- *
GLUTATHIONE , *CATABOLISM , *NON-small-cell lung carcinoma , *MITOCHONDRIA , *CYSTEINE - Published
- 2022
- Full Text
- View/download PDF
44. NRF2 Promotes Tumor Maintenance by Modulating mRNA Translation in Pancreatic Cancer.
- Author
-
Chio, Iok In Christine, Jafarnejad, Seyed Mehdi, Ponz-Sarvise, Mariano, Park, Youngkyu, Rivera, Keith, Palm, Wilhelm, Wilson, John, Sangar, Vineet, Hao, Yuan, Öhlund, Daniel, Wright, Kevin, Filippini, Dea, Lee, Eun Jung, Da Silva, Brandon, Schoepfer, Christina, Wilkinson, John Erby, Buscaglia, Jonathan M., DeNicola, Gina M., Tiriac, Herve, and Hammell, Molly
- Subjects
- *
MESSENGER RNA , *PANCREATIC cancer treatment , *DRUG efficacy , *LEUCINE zippers , *LUNG cancer - Abstract
Summary Pancreatic cancer is a deadly malignancy that lacks effective therapeutics. We previously reported that oncogenic Kras induced the redox master regulator Nfe2l2/Nrf2 to stimulate pancreatic and lung cancer initiation. Here, we show that NRF2 is necessary to maintain pancreatic cancer proliferation by regulating mRNA translation. Specifically, loss of NRF2 led to defects in autocrine epidermal growth factor receptor (EGFR) signaling and oxidation of specific translational regulatory proteins, resulting in impaired cap-dependent and cap-independent mRNA translation in pancreatic cancer cells. Combined targeting of the EGFR effector AKT and the glutathione antioxidant pathway mimicked Nrf2 ablation to potently inhibit pancreatic cancer ex vivo and in vivo, representing a promising synthetic lethal strategy for treating the disease. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
45. Depletion of a Putatively Druggable Class of Phosphatidylinositol Kinases Inhibits Growth of p53-Null Tumors.
- Author
-
Emerling, Brooke?M., Hurov, Jonathan?B., Poulogiannis, George, Tsukazawa, Kazumi?S., Choo-Wing, Rayman, Wulf, Gerburg?M., Bell, Eric?L., Shim, Hye-Seok, Lamia, Katja?A., Rameh, Lucia?E., Bellinger, Gary, Sasaki, Atsuo?T., Asara, John?M., Yuan, Xin, Bullock, Andrea, DeNicola, Gina?M., Song, Jiaxi, Brown, Victoria, Signoretti, Sabina, and Cantley, Lewis?C.
- Subjects
- *
PHOSPHATIDYLINOSITOL 3-kinases , *P53 protein , *GENETICS of breast cancer , *GENE expression , *REACTIVE oxygen species , *GENETIC mutation - Abstract
Summary: Here, we show that a subset of breast cancers express high levels of the type 2 phosphatidylinositol-5-phosphate 4-kinases α and/or β (PI5P4Kα and β) and provide evidence that these kinases are essential for growth in the absence of p53. Knocking down PI5P4Kα and β in a breast cancer cell line bearing an amplification of the gene encoding PI5P4K β and deficient for p53 impaired growth on plastic and in xenografts. This growth phenotype was accompanied by enhanced levels of reactive oxygen species (ROS) leading to senescence. Mice with homozygous deletion of both TP53 and PIP4K2B were not viable, indicating a synthetic lethality for loss of these two genes. Importantly however, PIP4K2A −/− , PIP4K2B +/− , and TP53 −/− mice were viable and had a dramatic reduction in tumor formation compared to TP53 −/− littermates. These results indicate that inhibitors of PI5P4Ks could be effective in preventing or treating cancers with mutations in TP53. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
46. In Vivo Identification of Tumor- Suppressive PTEN ceRNAs in an Oncogenic BRAF-Induced Mouse Model of Melanoma
- Author
-
Karreth, Florian A., Tay, Yvonne, Perna, Daniele, Ala, Ugo, Tan, Shen Mynn, Rust, Alistair G., DeNicola, Gina, Webster, Kaitlyn A., Weiss, Dror, Perez-Mancera, Pedro A., Krauthammer, Michael, Halaban, Ruth, Provero, Paolo, Adams, David J., Tuveson, David A., and Pandolfi, Pier Paolo
- Subjects
- *
TUMOR suppressor proteins , *ONCOGENES , *MELANOMA , *NON-coding RNA , *GENETIC regulation , *GENETIC transcription , *MUTAGENESIS , *LABORATORY mice - Abstract
Summary: We recently proposed that competitive endogenous RNAs (ceRNAs) sequester microRNAs to regulate mRNA transcripts containing common microRNA recognition elements (MREs). However, the functional role of ceRNAs in cancer remains unknown. Loss of PTEN, a tumor suppressor regulated by ceRNA activity, frequently occurs in melanoma. Here, we report the discovery of significant enrichment of putative PTEN ceRNAs among genes whose loss accelerates tumorigenesis following Sleeping Beauty insertional mutagenesis in a mouse model of melanoma. We validated several putative PTEN ceRNAs and further characterized one, the ZEB2 transcript. We show that ZEB2 modulates PTEN protein levels in a microRNA-dependent, protein coding-independent manner. Attenuation of ZEB2 expression activates the PI3K/AKT pathway, enhances cell transformation, and commonly occurs in human melanomas and other cancers expressing low PTEN levels. Our study genetically identifies multiple putative microRNA decoys for PTEN, validates ZEB2 mRNA as a bona fide PTEN ceRNA, and demonstrates that abrogated ZEB2 expression cooperates with BRAFV600E to promote melanomagenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.