18 results on '"Garces, Hans Garcia"'
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2. Infections Caused by Fusarium Species in Pediatric Cancer Patients and Review of Published Literature
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Arnoni, Mariana Volpe, Paula, Claudete Rodrigues, Auler, Marcos Ereno, Simões, Cirilo Cesar Naozuka, Nakano, Shirley, Szeszs, Maria Walderez, Melhem, Márcia de Souza Carvalho, Pereira, Virgínia Bodelão Richini, Garces, Hans Garcia, Bagagli, Eduardo, Silva, Eriques Gonçalves, de Macêdo, Melissa Ferreira, and Ruiz, Luciana da Silva
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- 2018
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3. Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples
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de Oliveira, Luciana Bonome Zeminian, primary, Della Coletta, Amanda Manoel, additional, Gardizani, Taiane Priscila, additional, Garces, Hans Garcia, additional, Bagagli, Eduardo, additional, Trilles, Luciana, additional, Barrozo, Ligia Vizeu, additional, Marques, Sílvio de Alencar, additional, Faveri, Julio De, additional, and Dias-Melicio, Luciane Alarcão, additional
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- 2023
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4. Aspects related to biofilm production and antifungal susceptibility of clinically relevant yeasts of the genus Trichosporon
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Lara, Bruna Rossini, primary, de Camargo, Bruno Braidotti, additional, Paula, Claudete Rodrigues, additional, de Morais Monari, Gabrielle Pires, additional, Garces, Hans Garcia, additional, Arnoni, Mariana Volpe, additional, Silveira, Mônica, additional, Gimenes, Viviane Mazo Fávero, additional, Junior, Diniz Pereira Leite, additional, Bonfietti, Lucas Xavier, additional, Oliveira, Lidiane, additional, de Souza Carvalho Melhem, Márcia, additional, Auler, Marcos, additional, Ramos, Regina Teixeira Barbieri, additional, Dias, Amanda Latercia Tranches, additional, Silva, Naiara Chaves, additional, Moreira, Débora, additional, Richini-Pereira, Virgínia Bodelão, additional, Anversa, Laís, additional, and da Silva Ruiz, Luciana, additional
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- 2023
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5. Comparing the phenotypic, genotypic, and proteomic identification of Trichosporon species: A globally emerging yeast of medical importance
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Lara, Bruna Rossini, primary, de Camargo, Bruno Braidotti, additional, Paula, Claudete Rodrigues, additional, Junior, Diniz Pereira Leite, additional, Garces, Hans Garcia, additional, Arnoni, Mariana Volpe, additional, Silveira, Mônica, additional, Gimenes, Viviane Mazo Fávero, additional, Siqueira, Lumena Pereira Machado, additional, Takahashi, Juliana Possatto Fernandes, additional, Melhem, Márcia de Souza Carvalho, additional, Richini-Pereira, Virgínia Bodelão, additional, Anversa, Laís, additional, and Ruiz, Luciana da Silva, additional
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- 2021
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6. Outbreak of equine pythiosis in a southeastern region of Brazil: Environmental isolation and phylogeny
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Paz, Giselle Souza da, primary, Camargo, Gabriel Gasparini, additional, Cury, José Eduardo, additional, Apolonio, Emanuel Vitor Pereira, additional, Garces, Hans Garcia, additional, Prado, Ana Carolina do, additional, Chechi, Jessica Luana, additional, Oliveira, Alana Lucena, additional, Watanabe, Marcos Jun, additional, Bagagli, Eduardo, additional, and Bosco, Sandra de Moraes Gimenes, additional
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- 2021
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7. Paracoccidioides brasiliensis Isolated from Nine-Banded Armadillos (Dasypus novemcinctus) Reveal Population Structure and Admixture in the Amazon Basin
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Bagagli, Eduardo, primary, Matute, Daniel Ricardo, additional, Garces, Hans Garcia, additional, Tenório, Bernardo Guerra, additional, Garces, Adalberto Garcia, additional, Alves, Lucas Gomes de Brito, additional, Yamauchi, Danielle Hamae, additional, Hrycyk, Marluce Francisca, additional, Barker, Bridget Marie, additional, and Teixeira, Marcus de Melo, additional
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- 2021
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8. Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus Paracoccidioides
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Vinche, Adriele Dandara Levorato, primary, de- la-Cruz-Chacón, Iván, additional, González-Esquinca, Alma Rosa, additional, Silva, Julhiany de Fátima da, additional, Ferreira, Gisela, additional, Santos, Daniela Carvalho dos, additional, Garces, Hans Garcia, additional, Oliveira, Daniela Vanessa Moris de, additional, Marçon, Camila, additional, Cavalcante, Ricardo de Souza, additional, and Mendes, Rinaldo Poncio, additional
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- 2020
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9. Rabies virus and Histoplasma suramericanum coinfection in a bat from southeastern Brazil
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Menozzi, Benedito Donizete, primary, Paz, Giselle Souza, additional, Paiz, Laís Moraes, additional, Garces, Hans Garcia, additional, Adorno, Brunna Mayla Vasconcelos, additional, Almeida‐Silva, Fernando, additional, Zancope Oliveira, Rosely Maria, additional, Richini‐Pereira, Virgínia Bodelão, additional, Chechi, Jéssica Luana, additional, Bagagli, Eduardo, additional, Bosco, Sandra de Moraes Gimenes, additional, and Langoni, Helio, additional
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- 2019
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10. Prp8 intein in dermatophytes: molecular identification and evolution
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Garces, Hans Garcia, Universidade Estadual Paulista (Unesp), and Bagagli, Eduardo [UNESP]
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filogenia ,Dermatophytes ,Intein PRP8 ,molecular identification ,PRP8 intein ,Dermatófitos ,Identificação molecular ,phylogeny - Abstract
Submitted by HANS GARCIA GARCES null (atiweb@gmail.com) on 2018-02-15T14:17:44Z No. of bitstreams: 1 tese final hans garcia.pdf: 5207018 bytes, checksum: dc8235a547fe1b9abb0de219b802a19f (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2018-02-20T17:09:18Z (GMT) No. of bitstreams: 1 garces_hg_dr_bot_par.pdf: 414383 bytes, checksum: a87b367ff5d75b8f43af0ee14876ad21 (MD5) Made available in DSpace on 2018-02-20T17:09:18Z (GMT). No. of bitstreams: 1 garces_hg_dr_bot_par.pdf: 414383 bytes, checksum: a87b367ff5d75b8f43af0ee14876ad21 (MD5) Previous issue date: 2018-02-08 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Os dermatófitos são um grupo de fungos constituídos pelos gêneros Trichophyton, Epidermophyton e Microsporum que têm a habilidade de degradar a queratina. É por essa razão que podem colonizar a pele do homem e dos animais, embora também possam crescer no ambiente, geralmente em solos com restos de queratina. As características morfológicas permitem a identificação e classificação taxonômica das espécies, mas realizar um diagnóstico baseado nestas características pode levar a erros. As técnicas moleculares ajudam a realizar diagnósticos mais rápidos baseados em uma identificação molecular e também têm possibilitado importantes avanços quanto aos estudos filogenéticos, sendo as sequências ITS1-5.8S-ITS2 as mais utilizadas. No entanto, estes marcadores não são suficientes para identificar e elucidar todas as relações filogenéticas e taxonômicas dos dermatófitos devido à ampla variedade de espécies existentes, -sendo necessário novos marcadores genéticos, como o intein PRP8. Os inteins são elementos genéticos parasitas, de natureza proteica, presentes em alguns fungos e estão associados a importantes genes altamente conservados. Ointein PRP8 está localizado nogenePRP8 que codifica para a proteína PRP8 associada ao complexo do spliciosoma. O presente estudo visa caracterizar o intein PRP8 em fungos dermatófitos, de forma a empregar estes elementos genéticos como marcadores moleculares para uma correta identificação destas espécies e elucidações das relações filogenéticas do grupo. Para realizar este objetivo, foram caracterizadasmolecularmente 45 cepas usando as regiões nucleares ribossomais ITS1-5.8S-ITS2 e D1/D2 e posteriormente a região do intein PRP8 de 40 cepas foi sequenciada para determinar as características do intein. A presença do intein foi comprovada em todas as espécies avaliadas, apresentando um full-intein com uma Homing Endonuclease presumivelmente ativa. As construções filogenéticas obtidas usando as sequências do intein são correspondentes as construídas a partir de genes nucleares ribossomais, demonstrando a validade do intein para ser incluído em futuros estudos filogenético associados a taxonomia e determinação de espécies. O polimorfismo na região da Homing Endonuclease permitiu a identificação de algumas espécies de Microsporum mediante um ensaio de PCR-Eletroforese. Dermatophytes are a fungal group composed by the genera Trichophyton, Epidermophyton and Microsporum with the ability to degrade keratin. That is why they can colonize the skin of man and animals, although they can also grow in the environment, usually in soils with remains of keratin. The morphological characteristics allow the identification and taxonomic classification of the species, but a diagnosis based on these characteristics is difficult. Molecular techniques made diagnoses based on molecular identification faster and made possible important advances in phylogenetic studies, with ITS1-5.8s-ITS2 sequences being the most used. However, these markers are not enough to elucidate all the phylogenetic relationships and taxonomic of dermatophytes due to the wide variety of existing species, and new genetic markers may be needed for correct identification and phylogenetic studies, such as the PRP8 intein. Inteins are parasitic genetic elements of a protein nature present in some fungi and are associated with important highly conserved genes. The PRP8 intein is located within the PRP8 gene coding for the PRP8 protein associated with the spliciosome complex. The present study aims to characterize the PRP8 intein in dermatophyte fungi, in order to use these genetic elements as molecular markers for a correct identification of these species and elucidations of the phylogenetic relationships of the group. To accomplish this goal, 45 strains were molecularly characterized using the ITS1-5.8S-ITS2 and D1 / D2 ribosomal nuclear regions and subsequently the PRP8 intein region of 40 strains was sequenced to determine the intein characteristics. The presence of intein was confirmed in all evaluated species, presenting a full-intein with a presumably active Homing Endonuclease. The phylogenetic construction obtained using the intein sequences correspond to those constructed from ribosomal nuclear genes, demonstrating the intein validity to be included in future phylogenetic studies associated with taxonomy and species determination. Polymorphism in the Homing Endonuclease region allowed the identification of some Microsporum species by a PCR-Electrophoresis assay. 12481-13-0
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- 2018
11. Apiotrichum veenhuisii isolated from a pediatric patient with acute myeloid leukemia: The first case in humans
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Lara, Bruna Rossini, primary, Melo, Milena Barrocali de Araújo, additional, Paula, Claudete Rodrigues, additional, Arnoni, Mariana Volpe, additional, Simões, Cirilo Cesar Naozuka, additional, Nakano, Shirley, additional, Richini-Pereira, Virgínia Bodelão, additional, Garces, Hans Garcia, additional, Maciel da Silva, Bosco Christiano, additional, Anversa, Laís, additional, Gonçalves Silva, Eriques, additional, Auler, Marcos Ereno, additional, Oliveira dos Santos, Rennan Luiz, additional, and da Silva Ruiz, Luciana, additional
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- 2019
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12. Rabies virus and Histoplasma suramericanum coinfection in a bat from southeastern Brazil.
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Menozzi, Benedito Donizete, Paz, Giselle Souza, Paiz, Laís Moraes, Garces, Hans Garcia, Adorno, Brunna Mayla Vasconcelos, Almeida‐Silva, Fernando, Zancope Oliveira, Rosely Maria, Richini‐Pereira, Virgínia Bodelão, Chechi, Jéssica Luana, Bagagli, Eduardo, Bosco, Sandra de Moraes Gimenes, and Langoni, Helio
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RABIES virus ,EMERGING infectious diseases ,MIXED infections ,BATS ,CITY dwellers ,PSYCHIATRIC hospitals ,PUBLIC hospitals - Abstract
Bats are essential to the global ecosystem, but their ability to harbour a range of pathogens has been widely discussed, as well as their role in the emergence and re‐emergence of infectious diseases. This paper describes the first report of coinfection by two zoonotic agents, rabies virus (RABV) and the fungus Histoplasma suramericanum in a bat. The bat was from the Molossus molossus species, and it was found during the daytime in the hallway of a public psychiatric hospital in a municipality in São Paulo State, southeastern Brazil. RABV infection was diagnosed by the direct fluorescent antibody test and mouse inoculation test. The fungus was isolated by in vitro culture. Both diagnoses were confirmed by molecular techniques. Phylogenetic analysis showed that the fungus isolate had proximity to H. suramericanum in the Lam B clade, while the RABV isolate was characterized in the Lasiurus cinereus lineage. Since the M. molossus bat was found in a peri‐urban transition area (urban/peri‐urban), the possibility of cross‐species transmission of this RABV lineage becomes more plausible, considering that this scenario may provide shelter for both M. molossus and L. cinereus. These are relevant findings since there has been an increase in bat populations in urban and peri‐urban areas, particularly due to environmental modifications and anthropogenic impacts on their habitat. Thus, the detection of two zoonotic agents in a bat found in a public hospital should raise awareness regarding the importance of systematic surveillance actions directed towards bats in urban areas. [ABSTRACT FROM AUTHOR]
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- 2020
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13. Ecology of Paracoccidioides brasiliensis, P. lutzii and related species: infection in armadillos, soil occurrence and mycological aspects.
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Hrycyk, Marluce Francisca, Garces, Hans Garcia, Bosco, Sandra de Moraes Gimenes, Oliveira, Sílvio Luis de, Marques, Sílvio Alencar, and Bagagli, Eduardo
- Abstract
Paracoccidioides brasiliensis and the related species P. americana, P. restrepiensis, P. venezuelensis, and P. lutzii (Ascomycota, Ajellomycetaceae) are the etiological agents of paracoccidoidoimycosis (PCM), one of the most important systemic mycoses in Latin America. They are dimorphic fungi, with a mycelial life cycle in soil and a yeast phase associated with tissues of mammalian hosts. This study aimed to detect Paracoccidioides spp. in armadillo tissues and associated soil samples in three well-defined geographic areas, including the Alta Floresta, an area not only endemic for PCM in the central region of Brazil but also of probable P. lutzii occurrence, whose ecology and geographic distribution are poorly elucidated. The isolates were genotyped by sequencing ITS-rDNA and the gp43 -exon-2 region, and by PCR-RFLP of alpha tubulin (tub1) gene; mycological aspects such as yeast-to-mycelial transition, growth and conidial production in soil extract agar were also evaluated. We confirmed that while armadillos are highly infected by P. brasiliensis, including multiple infections by distinct genotypes or species (P. brasiliensis and P. americana) in the same animal, the same does not hold true for P. lutzii, which in turn seems to present less capacity for mycelial growth and conidial production, when developing in a soil-related condition. [ABSTRACT FROM AUTHOR]
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- 2018
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14. PRP8 intein in dermatophytes: Evolution and species identification.
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Garces, Hans Garcia, Cordeiro, Raquel Theodoro, and Bagagli, E
- Abstract
Dermatophytes are keratinophilic fungi belonging to the family Arthrodermataceae. Despite having a monophyletic origin, its systematics has always been complex and controversial. Sequencing of nuclear ribosomal ITS and D1/D2 rDNA has been proposed as an efficient tool for identifying species in this group of fungi, while multilocus analyses have been used for phylogenetic species recognition. However, the search for new markers, with sequence and size variation, which enable species identification in only one polymerase chain reaction (PCR) step, is very attractive. Inteins seems to fulfill these characteristics. They are self-splicing genetic elements present within housekeeping coding genes, such as PRP8 , that codify the most important protein of the spliceosome. The PRP8 intein has been described for Microsporum canis in databases but has not been studied in dermatophytes in any other published work. Thus, our aim was to determine the potential of this intervening element for establishing phylogenetic relationships among dermatophytes and for identifying species. It was found that all studied species have a full-length PRP8 intein with a Homing Endonuclease belonging to the family LAGLIDADG. Phylogenetic analyses were consistent with other previous phylogenies, confirming Epidermophyton floccosum in the same clade of the Arthroderma gypseum complex, Microsporum audouinii close to M. canis , differentiating A. gypseum from Arthroderma incurvatum , and in addition, better defining the Trichophyton interdigitale and Trichophyton rubrum species grouping. Length polymorphism in the HE region enables identification of the most relevant Microsporum species by a simple PCR-electrophoresis assay. Intein PRP8 within dermatophytes is a powerful additional tool for identifying and systematizing dermatophytes. [ABSTRACT FROM AUTHOR]
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- 2018
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15. Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius.
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Furtado, Fabiana Barcelos, Borges, Bruna Cristina, Teixeira, Thaise Lara, Garces, Hans Garcia, Almeida Junior, Luiz Domingues de, Alves, Fernanda Cristina Bérgamo, Silva, Claudio Vieira da, and Fernandes Junior, Ary
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ESSENTIAL oils ,AROMATIC plants ,EVERGREENS ,ANTIFUNGAL agents ,ANTIBACTERIAL agents ,CHEMICAL composition of plants - Abstract
Natural products represent a source of biologically active molecules that have an important role in drug discovery. The aromatic plant Blepharocalyx salicifolius has a diverse chemical constitution but the biological activities of its essential oils have not been thoroughly investigated. The aims of this paper were to evaluate in vitro cytotoxic, antifungal and antibacterial activities of an essential oil from leaves of B. salicifolius and to identify its main chemical constituents. The essential oil was extracted by steam distillation, chemical composition was determined by gas chromatography/mass spectrometry, and biological activities were performed by a microdilution broth method. The yield of essential oil was 0.86% (w/w), and the main constituents identified were bicyclogermacrene (17.50%), globulol (14.13%), viridiflorol (8.83%), γ-eudesmol (7.89%) and α-eudesmol (6.88%). The essential oil was cytotoxic against the MDA-MB-231 (46.60 μg·mL
-1 ) breast cancer cell line, being more selective for this cell type compared to the normal breast cell line MCF-10A (314.44 μg·mL-1 ). Flow cytometry and cytotoxicity results showed that this oil does not act by inducing cell death, but rather by impairment of cellular metabolism specifically of the cancer cells. Furthermore, it presented antifungal activity against Paracoccidioides brasiliensis (156.25 μg·mL-1 ) but was inactive against other fungi and bacteria. Essential oil from B. salicifolius showed promising biological activities and is therefore a source of molecules to be exploited in medicine or by the pharmaceutical industry. [ABSTRACT FROM AUTHOR]- Published
- 2018
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16. Apiotrichum veenhuisiiisolated from a pediatric patient with acute myeloid leukemia: The first case in humans
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Lara, Bruna Rossini, Melo, Milena Barrocali de Araújo, Paula, Claudete Rodrigues, Arnoni, Mariana Volpe, Simões, Cirilo Cesar Naozuka, Nakano, Shirley, Richini-Pereira, Virgínia Bodelão, Garces, Hans Garcia, Maciel da Silva, Bosco Christiano, Anversa, Laís, Gonçalves Silva, Eriques, Auler, Marcos Ereno, Oliveira dos Santos, Rennan Luiz, and da Silva Ruiz, Luciana
- Abstract
ABSTRACTThis is the first report of the yeast Apiotrichum veenhuisii(formerly Trichosporon veenhuisii) causing disease in humans; its virulence and in vitro behavior against antifungals were also studied. The sample was isolated from biopsy fragments of disseminated lesions on the skin of a pediatric patient with acute myeloid leukemia. The studied virulence factors evidenced that the strain tested negative for secretion of the enzymes proteinase, phospholipase, and hemolysin. The isolate was characterized as low biofilm producer. Except for amphotericin B and voriconazole, the sample presented high minimum inhibitory concentration values against azole and echinocandins.
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- 2019
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17. Evolution of species of the genus sporothrix based on the sequences of vma and prp8 inteins
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Oliveira, Alana Lucena, Universidade Estadual Paulista (Unesp), Bosco, Sandra de Moraes Gimenes [UNESP], and Garces, Hans Garcia
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Sporotrix spp ,Filogenia ,Phylogen ,Intein VMA ,Diagnóstico molecular ,Intein PRP8 ,Molecular diagnosis - Abstract
Submitted by Alana Lucena Oliveira (alana.lucena@unesp.br) on 2021-02-01T15:48:01Z No. of bitstreams: 1 Dissertação Alana Lucena Oliveira 2020.pdf: 2308331 bytes, checksum: bd160b6361208756396acd7a6fa7d08b (MD5) Approved for entry into archive by ROSANGELA APARECIDA LOBO null (rosangelalobo@btu.unesp.br) on 2021-02-02T18:55:17Z (GMT) No. of bitstreams: 1 oliveira_al_me_bot_par.pdf: 2455167 bytes, checksum: f38020f6125c00265711f879b2caa596 (MD5) Made available in DSpace on 2021-02-02T18:55:17Z (GMT). No. of bitstreams: 1 oliveira_al_me_bot_par.pdf: 2455167 bytes, checksum: f38020f6125c00265711f879b2caa596 (MD5) Previous issue date: 2020-10-30 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) A esporotricose é uma micose causada principalmente pelas espécies do clado patogênico do gênero Sporothrix. Estas espécies compreendem S. schenckii, S. brasiliensis, S. globosa e S. luriei. S. schenckii tem uma ampla distribuição mundial enquanto S. globosa é uma sapronose comum na Ásia e S. luriei é uma espécie bem restrita e com pouquíssimos relatos. A esporotricose causada por S. brasiliensis é uma zoonose que afeta principalmente os gatos, cuja incidência aumentou no Brasil na última década, sendo considerada uma das micoses emergentes mais importantes na atualidade. Visando a eliminação de surtos, junto a campanhas de prevenção e medidas de vigilância, é muito importante contar com métodos diagnósticos de baixo custo, rápidos e precisos compreendendo as corretas espécies envolvidas. Foi realizada a padronização e diferenciação das espécies do clado patogênico do gênero Sporothrix por meio de biologia molecular utilizando regiões gênicas específicas dos inteins VMA e PRP8 que ainda não foram estudas para essas espécies. A região dos inteins VMA e PRP8 já foram utilizadas com finalidades de diagnóstico e estudos filogenéticos em outros fungos, sendo, portanto, uma região promissora para esses estudos no gênero Sporothrix Inteins são sequencias de proteínas presentes em alguns organismos que têm a capacidade de realizar um processo de auto-splicing logo após a tradução da sua proteína hospedeira. Sendo comprovada a existência dos dois inteins (VMA e PRP8) na maioria das espécies do clado patogênico de Sporothrix spp., o objetivo foi caracterizar os inteins PRP8 e VMA visando inferências diagnósticas e evolutivas, incluindo amostras de DNA a partir de isolados em cultivo . Até então, analisamos 22 cepas de isolados clínicos (5 S. globosa, 5 S. schenckii, 12 S. brasiliensis) pelo sequenciamento das regiões dos inteins com primers por nós desenhados e analisamos por bioinformática uma cepa de S. luriei. O intein PRP8 foi encontrado em todas as espécies enquanto o VMA foi ausente em S. globosa e S. luriei. Os inteins sempre foram encontrados na forma de Full-length intein. As análises filogenéticas permitiram a diferenciação das espécies de S. schenckii próximo a S. brasiliensis e S. globosa mais próxima de S. luriei ao analisar as sequencias do intein PRP8. Igualmente, o intein VMA evidenciou um agrupamento separado das espécies S. schenckii e S. brasiliensis. A presença ou ausência e análise do polimorfismo encontrado no intein VMA permitiu desenhar um par de primers adicional para diferenciar as espécies S. schenckii, S. brasiliensis e S. globosa por meio de PCR/eletroforese, sem necessidade de sequenciamento de DNA. Sporotrichosis is a fungal disease caused mainly by species of the pathogenic clade of the genus Sporothrix. These species comprise S. schenckii, S. brasiliensis, S. globosa and S. luriei. S. schenckii has a broad worldwide distribution while S. globosa is a common sapronosis in Asia and S. luriei is a very restricted species with very few reports associated with disease. S. brasiliensis is a zoonosis that mainly affects cats and has had a great incidence in Brazil in the last decade, being considered one of the most important emerging mycoses in recent times. In order to eliminate outbreaks, along with prevention campaigns and surveillance measures, it is very important to have low-cost, fast and accurate diagnostic methods, identifying the correct species involved. Standardization and differentiation of the species from the pathogenic clade of the complex genus Sporothrix were carried out by means of molecular biology using specific gene regions of the VMA and PRP8 inteins that have not yet been studied within these species. The VMA and PRP8 intein regions have already been used for the purposes of diagnosis and phylogenetic studies in other fungi, and are thus considered promising regions for the genus Sporothrix. Inteins are protein sequences present in some organisms that have the ability to perform a self-splicing process just after their host protein is translated. Since the existence of both inteins is proven in the majority of all species of the Sporothrix pathogenic clade, we aim to characterize the PRP8 and VMA inteins in order to diagnose these species molecularly and to determine the evolutionary history of the group, including DNA samples from cultivated isolates. Currently, we have 22 strains of clinical isolates (5 S. globosa, 5 S. schenckii, 12 S. brasiliensis) by sequencing the regions with designed primers and one strain of S. luriei that was analyzed by bioinformatics. The PRP8 intein was found in all species while the VMA intein was absent in S. globosa and S. luriei. Inteins was always found in full-length form. Phylogenetic analyses allowed species differentiation by grouping S. schenckii close to S. brasiliensis and S. globosa close to S. luriei when analyzing the sequences of the PRP8 intein. Likewise, the VMA intein showed a separate clustering of the species S. schenckii and S. brasiliensis. The absence of inteins in S. globosa and the polymorphism found in the VMA intein allowed the designing of an additional primer set to differentiate the species S. schenkii, S. brasiliensis and S. globosa thought only a PCR/Eletrophoresis technique without the need for sequencing. FAPESP: 2019/3489-7
- Published
- 2020
18. Outbreak of equine pythiosis in a southeastern region of Brazil: Environmental isolation and phylogeny.
- Author
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Paz GSD, Camargo GG, Cury JE, Apolonio EVP, Garces HG, Prado ACD, Chechi JL, Oliveira AL, Watanabe MJ, Bagagli E, and Bosco SMG
- Subjects
- Animals, Brazil epidemiology, Dogs, Horses, Phylogeny, Disease Outbreaks veterinary, Horse Diseases epidemiology, Horse Diseases microbiology, Pythiosis epidemiology, Pythium classification
- Abstract
Pythiosis is a disease caused by the oomycete Pythium insidiosum, mainly reported in equines, dogs and humans and directly transmitted through contaminant zoospores in aquatic environments. We report the first outbreak of equine pythiosis in five equines. Wound samples were submitted for diagnostic testing including mycological culture and nested PCR. Treatment approaches consisted of conventional and alternative therapies. Microbiological analyses were performed using water samples from the riverbanks close to where the animals had grazed. All animals were positive for P. insidiosum cultures, and two animals responded successfully to alternative therapy (ozone therapy). After culture and molecular analysis of environmental samples, the presence of P. insidiosum in one section of the Tietê River was confirmed through a 99% sequence identity. Phylogenetic analyses using the cytochrome oxidase II gene showed that the animal isolates clustered in clade I and the environmental isolates clustered in clade III. Although the environmental and wound isolates belonged to different genetic clades, we concluded that the Tietê River is an important source of infection by P. insidiosum and that research concerning environmental isolation of P. insidiosum from rivers and lakes should be strongly facilitated in Brazil., (© 2021 Wiley-VCH GmbH.)
- Published
- 2022
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- View/download PDF
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