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Objetivo: correlacionar la asertividad con el autocuidado de personas con diabetes mellitus tipo 2. Metodología: será de tipo transversal correlacional. El tamaño de muestra será de 198 participantes con DMT2, al cual se incluyó el 15% de tasa de no respuesta. El tipo de muestreo que se utilizará es no probabilístico por conveniencia. Para la recolección de la información se utilizarán los cuestionarios: Escala multidimensional de Asertividad y Resumen de las actividades del autocuidado en diabetes. Relevancia social: La prevención de las complicaciones micro y macrovasculares en la DMT2 es necesario realizar las acciones de autocuidado. Sin embargo, el autocuidado es influenciado por factores personales, familiares, conductuales, culturales y sociales. Dentro de los factores que no se ha evaluado es la asertividad. Ser asertivo fortalece la autoestima, brinda seguridad, facilita la libertad emocional, ayuda a resolver problemas y mejora la comunicación, lo que pudiera ayudar a los pacientes a tomar decisiones asertivas sobre su cuidado. Futuras intervenciones de enfermería podrán realizar acciones para mejorar las habilidades asertivas y permitir que mejore la toma de decisiones pertinentes para el mantenimiento o mejora de su salud., Objetive: to correlate assertiveness with the self-care of people with type 2 diabetes mellitus. Methodology: cross-sectional and correlational design. The sample size will be 198 participants with type 2 diabetes mellitus, which included a 15% non-response rate. The type of sampling will be is not probabilistic by convenience. For the collection of the information, the questionnaires will be used: Multidimensional Assertiveness Scale and Summary of self-care activities in diabetes. Social relevance: The prevention of micro and macrovascular complications in DMT2 is necessary to perform self-care actions. However, self-care is influenced by personal, family, behavioral, cultural and social factors. One of the factors that has not been evaluated is assertiveness. Being assertive strengthens self-esteem, provides security, facilitates emotional freedom, helps solve problems and improves communication, which could help patients make assertive decisions about their care. Future nursing interventions may carry out actions to improve assertive skills and allow for the improvement of relevant decisions for the maintenance or improvement of their health.

The composition and development of microbial population during the manufacture and ripening of two batches of a blue-veined cheese was examined by culturing and polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) (PCR-DGGE). Nine selective and/or differential media were used to track the cultivable populations of total and indicator microbial groups. For PCR-DGGE, the V3 hyper variable region of the bacterial 16S rRNA gene and the eukaryotic D1 domain of 28S rDNA were amplified with universal primers, specific for prokaryotes and eukaryotes, respectively. Similarities and differences between the results obtained by the culturing and the molecular method were recorded for some populations. Culturing analysis allows minority microbial groups (coliforms, staphylococci) to be monitored, although in this study PCR-DGGE identified a population of Streptococcus thermophilus that went undetected by culturing. These results show that the characterization of the microbial populations interacting and evolving during the cheese-making process is improved by combining culturing and molecular methods. © 2010 Springer Science+Business Media, LLC.