Search

Based on the SM2 digital signature standard,a proxy-protected proxy signature scheme was designed.Through the design of the agent authorization mechanism,the protection of the proxy signer was realized.The proxy signature was realized by the proxy signer who represents the original signer through the improvement of the SM2 digital signature algorithm.The security analysis shows that the designed algorithm is verifiable,identifiable,distinguishable,non-repudiation,and strong unforgeability.Finally,the program was implemented using the C language and the Polarssl library,and the performance was compared with the SM2 digital signature.The analysis shows that the algorithm has good usability in performance.

The effects of phosphate fertilizer application rates on the yield of sweet potato variety Longshu 601 (a cultivar with early maturity and high yield) were studied, in order to provide technical support for the realization of combining good methods with improved varieties and promoting the demonstration and promotion of new varieties. In this experiment, the single-factor randomized block design was used to analyze the effects of different phosphate fertilizer application rates (0, 37, 5, 75, 112.5, 150, and 300 kg · hm-2) on the agronomic traits, yield and yield components of sweet potato with no fertilization treatment as the blank control (CK). The results showed that compared with no fertilization, the commercial potato rate of sweet potato with the fertilization treatments was all significantly increased. There was a significant difference between the application of phosphate fertilizer (except 112. 5 kg · hm-2) and no application of phosphate fertilizer, and the potato yield treated with the application rate of 75. 0 kg · hm-2 and 150. 0 kg · hm-2 were higher. The yield treated with different fertilizer application rates was significantly higher than that treated with no fertilization (CK). Compared with no phosphate fertilization treatment (P2), the yield of Long-shu 601 treated with the appropriate phosphate fertilization was significantly increased, and the maximum yield was 61. 06 t · hm-2 when treated with the phosphate application rate of 112. 5 kg · hm-2 (P5). The quadratic curve function fitting was carried out between the phosphate fertilizer application amount and the yield of fresh potato of Longshu 601. The fitted equation was Y = -0. 000 34X²+0. 114 014X+49. 3138, r=0.9655. According to the simulated calculation of the peak of quadratic curve, the yield of fresh potato of Longshu 601 reached the highest (58.87 t · hm-2) when the application amount of phosphate fertilizer was 167. 67 kg · hm-2. The economic benefit of sweet potato could be improved by applying the proper amount of phosphate fertilizer, and the highest investment rate could be reached when treated with the phosphate fertilizer application rate of 112. 5 kg · hm-2 (P5). In other words, the investment rate of fertilizer was the highest (reaching 12.55) when applying the nitrogen fertilizer (N) of 90 kg · hm-2, potassium fertilizer (K2O) of 150 kg · hm-2 and phosphate fertilizer (P2O5) of 112. 5 kg · hm-2. [ABSTRACT FROM AUTHOR]

Expansions were carried out in finite element (FE) models of disc hernia including symmetric (median, lateral, paramedian) and asymmetric types. In all models, lubricous disk bulging that applied a linear compression to the anterior part of the cord was observed at the posterior surfaces of the expansion zone, respectively. The shape and position of protrusions varyed with the temperature, magnitude, and location of expanding elements. The geometric deformation and stress distribution of the spinal cord increased as the extent of compression grew. This method is in possession of enormous potential in promoting further individualized research of cervical spondylotic myelopathy. [ABSTRACT FROM AUTHOR]

A photocatalyst-free and EDA complex-enabled radical cascade cyclization reaction of inactive alkenes with bromodifluoroacetamides was reported for the divergent synthesis of fluorine-containing tetralones and quinazolinones. In this transformation, persulfates as electron donors and difluoro bromamide as electron acceptors generate the EDA complex. This is a promising photochemical method with advantages such as mild reaction conditions, simple operation, being metal-free, and excellent functional group tolerance.

Oral lichen planus (OLP) is a common chronic inflammatory disease of the oral mucosa. The prevalence rate of OLP in adults is 0.5%-2%. The etiology and pathogenesis of OLP are still unclear. The pathogenesis of OLP may be related to the genetic polymorphism of some genes. Currently, the gene families, including tumor necrosis factor, interferon, interleukin, enzyme, and receptor, have been extensively studied. This work reviews related studies on gene polymorphism of OLP.

Because of the anergy of CD25+CD4+ regulatory T cells, it is unclear how the number of these regulatory T cells is sustained and expanded in normal physiologic circumstances. In the present study, we examined the effect of natural allogeneic mature dendritic cells (DCs) on the proliferation and function of CD25+CD4+ T cells. Our data showed that natural allogeneic mature DCs stimulated CD25+CD4+ T-cell growth vigorously, whereas immature DCs had little effect on the proliferation of CD25+CD4+ T cells. After expansion by mature DCs, CD25+CD4+ T cells maintained their expression of Foxp3 and suppressed the proliferation of CD25- CD4+ T cells similar to freshly isolated CD25+CD4+ T cells. Our results introduce a potentially critical role played by natural allogeneic mature DCs, which exist in normal physiologic circumstances, in controlling CD25+CD4+ regulatory T-cell expansion and function.

We aimed to investigate whether tolerogenic dendritic cells (DCs) were induced in the tolerant recipients with the blockade of CD40-CD40L costimulation. Mouse heterotopic heart transplantation was performed. DCs were sorted from rejected and tolerant recipients using magnetic-activated cell sorting. Their expression of CD40, CD80, and CD86 was examined using fluorescence-activated cell sorting. DCs were stimulated with lipopolysaccharide in vitro, and interleukin 10 (IL-10) and IL-12 levels in the supernatants were evaluated using enzyme-linked immunosorbent assay. By using mixed leukocyte reaction, we investigated the stimulatory capacities and tolerogenic capability of DCs. DCs from tolerant recipients expressed lower level of costimulatory molecules, including CD40, CD80, and CD86 and released higher levels of IL-10 and lower levels of IL-12. In addition, DCs from tolerant recipients were weak stimulators of the mixed leukocyte reaction and inhibited the proliferation of splenocytes. IL-10(high)IL-12(low) DCs with immature phenotype were induced in the tolerant recipients with the blockade of CD40-CD40L costimulation, and they obtained the tolerogenic function.

Objective: To explore the clinical effect of combined application of magnetic attachments and extracoronal attachments in prosthodontics., Methods: Twenty-two cases of dentition defect with isolated residual root or residual crown were selected. All the cases accepted the restorative treatment combined magnetic attachments with extracoronal attachments. The clinical effect of dentures and the condition of abutment teeth were evaluated by chief complaint of patients and clinical examination. The follow-up time ranged from 2 years to 4 years., Results: Satisfactory functional, stable and esthetic results of dentures were achieved for all the cases. And there was no abutment loosening and no secondary caries. Alveolar bone loss around abutment occurred in 1 case, gingivitis of abutments appeared in 5 cases. Chewing pain occurred in 1 case and unstable state of dentures appeared in 5 cases after the use of denture for 2 years. The dentures of above-mentioned cases were used normally after symptomatic treatment., Conclusion: The combined application of magnetic attachments and extracoronal attachments in prosthodontics is an effective treatment option for cases of dentition defect with isolated residual root or residual crown.

Objective: To transduce the new hepatocellular carcinoma (HCC) specific antigen gene glypican3 (GPC3) into dendritic cells (DCs) and to observe the in vitro cytotoxic effect induced by the genetically modified DCs against the hepatocellular carcinoma cell line (HepG2)., Methods: This study was performed in China Medical University Shenyang, China from September 2007-February 2008. The design of the study was to modify DCs with GPC3 and to be used to activate human T cells and elicit a cell-mediated immune response against HepG2 in vitro. The GPC3 gene expression was identified by western blot and immunocytochemistry. The proliferation of responder cells and cytotoxicity against HepG2 were examined by water-soluble tetrazolium salt -1 and lactate dehydrogenase assay respectively. The interferon-y (IFN-y) secreted was detected by ELISA assay., Results: Both Western blot and immunocytochemical analysis assured the validity of GPC3 transfection. Glypican3 modified DCs were potent in inducing responder cells proliferation and IFN-y production. The cytotoxicity in the group of GPC3 transfected DCs were (38.90+/-0.95%) at the ratio of effector cells/target cells E/T:100:1, 30.83+/-1.24% at the ratio of E/T:50:1, and 23.84+/-0.65% at the ratio of E/T:10:1, respectively (which is significant compared with other groups, p<0.001). And the GPC3 modified DCs showed ability to induce high specific cytotoxicity against HepG2 in vitro., Conclusion: The effector cells stimulated with DCs that were transfected with pEF-hGPC3 plasmid could effectively lyse GPC3 expressing HepG2 cells, which suggested that those genetically engineered DCs have the potential to serve as novel vaccine for HCC.

In order to provide the evidence for the application of DYS413(YCAIII) locus in forensic medicine and population genetics by investigating its polymorphism in the Mongolians lived in the Inner Mongolia Autonomous Region, China, to collect 120 unrelated male Mongolians' venous blood, who lived in the Inner Mongolia Autonomous Region, and the blood is anticoagulant with EDTA, then to extract DNA by using the method of phenol-chloroform and amplify the DYS413 locus with PCR. The products were separated with 6% non-denatured polyacrylamide/bisacrylamide gels(19:1) electrophoresis and stained with 1 per thousand nitrate of silver(AgNO(3)). There are total 30 different haplotypes in Mongolians, the frequencies are from 0.0007 to 0.01361, the HD(haplotype diversity) is 0.94054. It shows that DYS413 locus is high valuable in the application of forensic medicine and population genetics.