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Children's obesity rates have increased substantially over the past several decades, due in part to unhealthy eating habits. About 75% of preschool-aged children consume fewer fruits and vegetables than recommended for health. Because children begin developing eating habits during early childhood, obesity prevention programs are increasingly targeting young children in early childhood education (ECE) settings and are involving their families in teaching about healthy eating. The purpose of this pilot study is to assess the effectiveness of a family involvement activity known as the family backpack in increasing fruit and vegetable consumption among 4- and 5-year-old children and their parents through hands-on activities that encourage them to explore and discuss fruits and vegetables at home. Forty-two families (22 experimental, 20 control) participated in the study. Families received a family backpack from their child's ECE teacher and completed activities with their child at home during a specific week. Experimental-group backpacks contained a children's book and three activities to encourage parent-child discussion about fruits and vegetables; control-group backpacks contained an unrelated book and activities. Children's and parents' fruit and vegetable consumption and frequency of serving fruits and vegetables at home were assessed using parent self-report surveys before and after completing backpack activities. Parents in the experimental group, but not the control group, reported increases in their own and their children's fruit and vegetable consumption after completing family backpack activities. Family backpacks show promise as a tool for early childhood educators to help families reinforce and expand children's learning and encourage healthy eating habits at home.

Digital music is one of the most important commodities on the market due to music royalty distribution in Korea. As the music market has been transformed into a digital music market by means such as downloading and streaming, the distribution of music royalties via online service providers (OSPs) has become a highly important issue for music rights holders. Currently, one of the most important issues in music royalty distribution in Korea is the unfair distribution of royalties due to the indiscriminate repeat streaming of digital music. To prevent this, music consumption log data from several OSPs were collected via a day-based system; however, there was a limit on the identification of detailed information on the use of music in its current state. This paper analyzes the structural problems and limitations related to the settlement of music royalties and provides a structure in which there can be transparent settlement and distribution between users and rights holders as an institutional measure. We also propose various AI (artificial intelligence)-based applications using music consumption log data. The proposed system will hopefully be used for public purposes. [ABSTRACT FROM AUTHOR]

As the community food environment is known to be an important factor in healthy food choices, it needs to be measured, to increase awareness and improve healthy eating. The objective of this study was to develop a perceived community food accessibility measurement (P-CFAM) questionnaire applicable to older adults in Korea and evaluate its reliability and validity. The questionnaire was developed based on community food environment factors that were found to affect food choices in previous studies. We evaluated the internal consistency reliability, construct validity, and criterion-related validity. The subjects were 188 older adults for validity. The Cronbach's alpha value for the reliability measure was 0.9. The confirmatory factor analysis to evaluate the construct validity of the questionnaire showed values close to those of the optimal model (GFI = 0.983, AGFI = 0.948, RMR = 0.004, NFI = 0.987, CFI = 0.996). Regarding the criterion-related validity, the P-CFAM questionnaire results were significantly related to objective measurement indicators such as the number of grocery stores, the travel time to a grocery store, and the intake of vegetables and fruit. In conclusion, the developed P-CFAM questionnaire was shown to be reliable and valid, and useful for assessing older adults' perceptions of community food accessibility in Korea. [ABSTRACT FROM AUTHOR]

In this study, we have examined the anticancer effects of SH005S7 on MET-amplified and (HCC827GR) NSCLC cells and their primary HCC827 cells. In vitro, first of all, cell viability and colony formation assay confirmed the growth inhibitory effects of SH005S7 on both cells. Second, SH005S7 inactivated EGFR-related multiple cell signaling, which was associated with a marked decrease in the constitutive phosphorylation of EGFR, HER3, MET, AKT, and ERK. Third, SH005S7 attenuated the anchorage-independent cell growth. Fourth, SH005S7 blocked invasive and metastatic capability by downregulation of mesenchymal markers—vimentin, snail, and MMP-9. Fifth, BrdU assay confirmed the cell cycle arrest of SH005S7 on these cells. When administered orally to nude mice xenografically transplanted human NSCLC, SH005S7 inhibited the growth of tumor and did not cause hepatotoxicity and nephrotoxicity in animals. Immunohistochemical and Western blot analyses of tissue showed that the suppression of growth correlated with inhibition of proliferation (Ki-67, PCNA), invasiveness (vimentin, snail), and angiogenesis (CD31) marker and decrement in the constitutive and phosphorylation of EGFR, HER3, MET, AKT, and ERK. Additionally, SH005S7 had immune stimulatory effects by TNF-α cytokine release on macrophage, without cell cytotoxicity. Overall, our results suggest that SH005S7 can inhibit the growth of MET-amplified and gefitinib-resistant NSCLC cells through the suppression of EGFR-related multiple targets linked to overcome gefitinib resistance. [ABSTRACT FROM AUTHOR]

An optical depth converter that uses a lens array pair is analyzed theoretically and experimentally. We present a theory of depth conversion and explain the effects of the system parameters in the optical depth converter by using wave-optical analysis. Ray-optical analysis is applied to the investigation of the tendencies of the system parameter effects. We also show that the optical depth converter can be used for the three-dimensional screen in projection-type integral imaging systems. OCIS codes: 110.2990, 100.6890, 220.2740.

This paper proposes a Gaussian process-based method for trajectory learning and generation of individualized gait motions at arbitrary user-designated walking speeds, intended to be used in generating reference motions for robotic gait rehabilitation systems. We utilize a nonlinear dimension reduction technique based on Gaussian process dynamical models (GPDMs), in which the internal dynamics is modeled as a second-order Markov process evolving in a lower-dimensional latent space. After the GPDM parameters are identified with training data obtained from gait motions of healthy subjects walking at different speeds, our method then employs Gaussian process regression (GPR) to predict the initial two states of the latent space dynamics from any arbitrary desired walking speed and the anthropometric parameters of the test subject. Motions are then generated by directly mapping the latent space dynamics to joint trajectories. Experimental studies involving more than 100 subjects indicate that our method generates gait patterns with 30% less mean square prediction errors compared to recent state-of-the-art methods, while also allowing for arbitrary user-specified walking speeds. [ABSTRACT FROM AUTHOR]

Staphylococcus aureus is an important pathogen implicated in various diseases, including staphylococcal food poisoning. Bacteriocins are considered safe and effective antimicrobial substances for the prevention of the growth of pathogenic bacteria. In this article, we describe the purification and characterization of pasteuricin, a novel bacteriocin produced by Staphylococcus pasteuri RSP-1. A cell-free supernatant of S. pasteuri RSP-1 exerted strong antimicrobial activity against staphylococci, including methicillin-resistant S. aureus (MRSA), and gram-positive bacteria. The loss of antimicrobial activity upon treatment with proteolytic enzymes confirmed the proteinaceous nature of pasteuricin. A rapid and pronounced bactericidal effect of pasteuricin was confirmed by a live-dead bacterial viability assay. To our knowledge, pasteuricin is the first reported S. pasteuri bacteriocin that inhibits S. aureus. Because pasteuricin is characterized by strong antimicrobial activity and high stability, it has potential as an alternative antimicrobial agent to antibiotics. [ABSTRACT FROM AUTHOR]

Coagulase-negative staphylococci (CNS) are opportunistic pathogens that are currently emerging as causative agents of human disease. Though CNS are widespread in the clinic and food, their precise identification at species level is important. Here, using 16S rRNA sequencing, 55 staphylococcal isolates were identified as S. capitis, S. caprae, S. epidermidis, S. haemolyticus, S. pasteuri, S. saprophyticus, S. warneri, and S. xylosus. Although 16S rRNA sequencing is universally accepted as a standard for bacterial identification, the method did not effectively discriminate closely related species, and additional DNA sequencing was required. The divergence of the sodA gene sequence is higher than that of 16S rRNA. To devise a rapid and accurate identification method, sodA-specific primers were designed to demonstrate that species-specific multiplex polymerase chain reaction (PCR) can be used for the identification of CNS species. The accuracy of this method was higher than that of phenotypic identification; the method is simple and less time-consuming than 16S rRNA sequencing. Of the 55 CNS isolates, 92.72% were resistant to at least one antibiotic, and 60% were resistant to three or more antibiotics. CNS isolates produced diverse virulence-associated enzymes, including hemolysin (produced by 69.09% of the isolates), protease (65.45%), lipase (54.54%), lecithinase (36.36%), and DNase (29.09%); all isolates could form a biofilm. Because of the increasing pathogenic significance of CNS, the efficient multiplex PCR detection method developed in this study may contribute to studies for human health. [ABSTRACT FROM AUTHOR]

Pointwise intensity-based algorithms are the most popular algorithms in dynamic laser speckle measurement of physical or biological activity. The output of this measurement is a two-dimensional map which qualitatively separates regions of higher or lower activity. In the paper, we have proposed filtering of activity maps to enhance visualization and to enable quantitative determination of activity time scales. As a first step, we have proved that the severe spatial fluctuations within the map resemble a signal-dependent noise. As a second step, we have illustrated implementation of the proposed idea by applying filters to non-normalized and normalized activity estimates derived from synthetic and experimental data. Statistical behavior of the estimates has been analyzed to choose the filter parameters, and substantial narrowing of the probability density functions of the estimates has been achieved after the filtering. The filtered maps exhibit an improved contrast and allowed for quantitative description of activity. [ABSTRACT FROM AUTHOR]

Micro-/nanofabrication-based lab-on-a-chip (LOC) technologies have recently been substantially advanced and have become widely used in various inter-/multidisciplinary research fields, including biological, (bio-)chemical, and biomedical fields. However, such hybrid-scale LOC devices are typically fabricated using microfabrication and nanofabrication processes in series, resulting in increased cost and time and low throughput issues. In this review, after briefly introducing the conventional micro-/nanofabrication technologies, we focus on unconventional micro-/nanofabrication technologies that allow us to produce either in situ micro-/nanoscale structures or master molds for additional replication processes to easily and conveniently create novel LOC devices with micro- or nanofluidic channel networks. In particular, microfabrication methods based on crack-assisted photolithography and carbon-microelectromechanical systems (C-MEMS) are described in detail because of their superior features from the viewpoint of the throughput, batch fabrication process, and mixed-scale channels/structures. In parallel with previously reported articles on conventional micro-/nanofabrication technologies, our review of unconventional micro-/nanofabrication technologies will provide a useful and practical fabrication guideline for future hybrid-scale LOC devices. [ABSTRACT FROM AUTHOR]

This paper presents design and implementation of a color wavefront holographic printer. The printer output is a white light viewable volume hologram composed as a two-dimensional (2-D) array of elemental holograms. The three-dimensional (3-D) input data for the printer are encoded as computer generated holograms (CGHs) displayed in succession on an amplitude type spatial light modulator. The printer optical head extracts and demagnifies the beam with the 3-D object information from the beam diffracted by the modulator to produce a small size elemental hologram. This allows for mosaic delivery of exposures at primary colors with each elemental hologram acting as a single color channel. Computer generation of digital contents is accelerated by design of a phase-added stereogram approach based on partitioning of each CGH. Bright 3-D reconstruction with a motion parallax at saturated colors from holograms of test objects is presented. The holograms are printed on a silver-halide emulsion. The achieved high quality proves feasibility of recording analog color volume holograms from digital contents by mosaic delivery of the primary colors. Two quality enhancement approaches are proposed and tested. [ABSTRACT FROM PUBLISHER]

Staphylococcus aureus is an important foodborne pathogen on global basis. The current study investigated the genetic patterns in S. aureus isolates from leaf vegetables ( n = 53). Additional isolates from livestock ( n = 31) and humans ( n = 27) were compared with the leaf vegetable isolates. Genes associated with toxins, antibiotic resistance, and pulsed-field gel electrophoresis (PFGE) patterns were analyzed. At least 1 enterotoxin-encoding gene ( sea, seb, sec, sed, and see) was detected in 11 of 53 (20.75%) leaf vegetable isolates. When the agr (accessory gene regulator) grouping was analyzed, agr II was the major group, whereas agr IV was not present in leaf vegetable isolates. All S. aureus isolates from leaf vegetables were resistant to more than one of the antibiotics tested. Nineteen of 53 (35.85%) isolates from leaf vegetables exhibited multidrug-resistance, and 11 of these were MRSA (methicillin-resistant S. aureus). A dendrogram displaying the composite types of S. aureus isolates from 3 origins was generated based on the combination of the toxin genes, agr genes, antibiotic resistance, and PFGE patterns. The isolates could be clustered into 8 major composite types. The genetic patterns of S. aureus isolates from leaf vegetables and humans were similar, whereas those from livestock had unique patterns. This suggests some S. aureus isolates from leaf vegetables to be of human origin. [ABSTRACT FROM AUTHOR]

Effective inhibition of microbial growth is essential to increase the yield and shelf life of food. In this study, non-thermal N 2 plasma-treated buffer (NPB) was tested for its ability to inactivate plant pathogenic bacteria. Our results showed that NPB strongly inhibited bacterial growth, and 100% inhibitory activity was maintained when up to 10-fold diluted NPB was tested. Scavenger assay using various antioxidants against specific reactive oxygen species (ROS) and reactive nitrogen species (RNS) showed that certain ROS and RNS in NPB were responsible for its strong antimicrobial activity. NPB inhibited the growth of plant pathogens on raw produce and was non-toxic to animal cells at all the concentrations tested. In conclusion, our study showed that NPB is a safe and effective antimicrobial agent against plant pathogenic bacteria, and may have wider applications to enhance crop production and the shelf life of stored food. • N 2 plasma treated buffer (NPB) shows strong inhibition of pathogenic bacteria. • Specific ROS in NPB are responsible for the pathogen-inhibition activity. • NPB has no significant cytotoxic effect on animal cell lines. • NPB holds potential for enhancing the shelf-life of stored food. [ABSTRACT FROM AUTHOR]

Controlling of biofilms formation in numerous pathogenic bacteria is one of the most difficult tasks in the control of bacterial diseases. Plasma has attracted extensive attention due to their potential applications for effective inhibiting of biofilm. Recently, plasma-activated water (PAW) has developed as an alternative method for bacterial inactivation and disinfection of foods owing to advantages of more convenient and efficient storage and transportation than direct plasma application. However, most previous studies about PAW have only focused on the improvement of its antibacterial effect instead of antibiofilm activity. Therefore, we report the development of N2 plasma treated buffer (NPB) and the super-antibiofilm effect of NPB against Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) as a plant pathogenic bacterium. Scavenger assays using various antioxidants revealed that reactive oxygen species were involved in the inhibitory cellular actions of NPB, with H2O2 and singlet oxygen proving essential for bacterial death. Intensive analysis of NPB, stored at different periods and temperatures, showed that the antimicrobial efficacy was well maintained for 3 months at − 80 °C. Importantly, further studies showed that NPB effectively inhibited not only the growth of planktonic Pst DC3000 but also biofilm formation. The remarkable inhibition on the biofilm was analyzed and visualized using LIVE/DEAD viability assays and confocal laser scanning microscopy (CLSM) imaging. The 3D CLSM imaging data revealed that the bactericidal activity of NPB was permeable enough to affect the cells embedded inside the biofilm. This prominent permeability could be a crucial feature of NPB contributing to effective super-antibiofilm. [ABSTRACT FROM AUTHOR]

We propose a novel scheme to provide a two-dimensional/three-dimensional convertible feature for integral imaging operation under real/virtual mode. With a new fabrication method for a concave (or convex) half mirror array, a prototype system was implemented to verify the feasibility of our proposed scheme. Experimental results show that the proposed scheme also provides a wider viewing angle of an integrated image than the conventional integral imaging system. It is expected that the proposed two-dimensional/three-dimensional convertible integral imaging scheme will be useful for providing an integrated image with high resolution and a wide viewing angle. [ABSTRACT FROM AUTHOR]