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Oncorhynchus mykiss is a high-quality, edible fish with high nutritional and economic value. Its tender meat, full elasticity, delicious flavor, and rich nutritional value make it favorite among consumers. However, owing to its high water and fat content and high enzymatic activity, O. mykiss is highly susceptible to spoilage during storage, transportation, and sale.To study the effects of different pretreatment methods on the quality of O. mykiss during storage and slow its decay rate, experiments were conducted with two pretreatment groups: Cold saltwater group (salt content 3%, temperature 4 ℃) and slightly acid electrolyzed water group (pH 6.35, ORP 900 mV, ACC 50 mg/L, room temperature). Different pretreatment times were set for each group: 20 min, 40 min, and 60 min of cold saltwater treatment and 5 min, 10 min, and 15 min of slightly acid electrolyzed water treatment. TVB-N value, total bacterial count, water holding capacity, cooking loss rate, pH value, chromaticity, and sensory evaluation were measured as indicators, and untreated O. mykiss meat was used as a control to compare the changes of the quality of O. mykiss meat during a 9-day storage experiment at 4 ℃.The results showed that the optimal pretreatment times were 60 min for the cold saltwater pretreatment and 10 min for the slightly acid electrolyzed water pretreatment. Both preprocessing methods can effectively maintain the freshness of O. mykiss and delay its spoilage. Compared with the cold saltwater pretreatment, the slightly acid electrolyzed water treatment better inhibited the total bacterial count and TVB-N generation of O. mykiss, maintaining its water-holding capacity and delaying the rate of change of pH, chromaticity, and sensory properties during storage. Cold saltwater pretreatment can extend the refrigerated shelf life of O. mykiss by 1 day, whereas slightly acid electrolyzed water pretreatment can extend the shelf life by 2 days.Overall, pretreatment with slightly acid electrolyzed water for 10 min can effectively improve the preservation quality of O. mykiss stored at 4 ℃. Slightly acid electrolyzed water has enormous potential for applications in the preservation of aquatic products. Its unique sterilization effect and safety make it preferred for aquatic product preservation. Slightly acid electrolyzed water can effectively inhibit the growth of microorganisms in aquatic products, extend shelf life, and maintain the freshness and taste of aquatic products. In the future, with advancements in technology and increasing awareness of food safety among consumers, slightly acid electrolyzed water will play an important role in the preservation of aquatic products, providing strong guarantees for the quality and safety of aquatic products.

Abstract Aim To explore the prevalence of turnover intentions among emergency nurses across the globe, decision-makers should be offered evidence-based assistance. Background and introduction Compared with those of general nurses, the unique work environment and pressure significantly impact emergency nurses’ turnover intention. High personnel turnover intention often hinders the provision of high-quality emergency services. Methods This study was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Published and unpublished papers were identified through electronic searches of PubMed, Web of Science, EMBASE, CINAHL, and the Cochrane Library from their establishment until February 1, 2023. The literature included in this study may encompass cross-sectional studies and longitudinal studies. Two researchers independently screened the literature, extracted data, and assessed the quality of the included studies while using the tool developed by Hoy and colleagues in 2012. Stata 17.0 was used for all the statistical analyses. Results This study included 12 articles by screening 744 articles, which included a total of 4400 nurses. All studies included in the analysis were cross-sectional. The overall prevalence of turnover intention among emergency nurses was 45%. Further analysis revealed that the turnover intention prevalence among emergency nurses in Asia was 54%, whereas in other regions, it was 38%. The turnover intention among younger nurses (61%) was significantly greater than that among older nurses (30%). Compared with the published scale, the self-developed scale resulted in a higher turnover intention rate of 52%, which was 41%. Conclusion The prevalence of emergency nurses’ turnover intention is relatively high and shows an increasing trend, with noticeable variations across different regions and age groups. Notably, Asian nurses and those younger than 35.6 years exhibit a greater intention to turnover. Patient or public contribution There is no patient or public involvement, as this article is a meta-analysis. Implications for nursing and health policy Nursing managers, administrators, and policymakers must recognize the seriousness of high turnover intentions among emergency nurses and develop effective prevention strategies to address this issue globally.

Objective The management of the infrapatellar fat pad (IPFP) during total knee arthroplasty (TKA) remains controversial. This study aimed to evaluate a novel IPFP preservation technique—“the medially pedicled IPFP flap”—for reducing postoperative pain, wound complications, and improving functional recovery after TKA. Methods A retrospective analysis was conducted on TKA cases at our institution from 2018 to 2021, including those with IPFP preservation (medially pedicled flap) versus IPFP complete resection. Patient demographics, perioperative parameters (blood loss, operative time, length of hospital stay, visual analogue scale [VAS] score, white cell count [WBC], C‐reactive protein [CRP], erythrocyte sedimentation rate [ESR], and wound oozing), and postoperative follow‐up data (VAS, Knee Society [KSS], or Knee Society functional assessment [KSFA] scores) were compared between groups. Independent sample t‐tests were used to compare continuous data and chi‐squared tests were used to compare categorical data between groups. Results Six hundred thirty patients were included, with 278 in the medial pedicled IPFP flap group (preservation group) and 352 in the IPFP resection group (resection group). The operative time was significantly shorter in the preservation versus resection group (125.5 ± 23.2 vs 130.3 ± 28.7 mins, p = 0.03), as was the length of hospital stay (8.4 ± 2.7 vs 9.2 ± 2.3 days, p

Abstract Background Seed amplification assays (SAA) enable the amplification of pathological misfolded proteins, including α-synuclein (αSyn), in both tissue homogenates and body fluids of Parkinson’s disease (PD) patients. SAA involves repeated cycles of shaking or sonication coupled with incubation periods. However, this amplification scheme has limitations in tracking protein propagation due to repeated fragmentation. Methods We introduced a modified form of SAA, known as Quiescent SAA (QSAA), and evaluated biopsy and autopsy samples from individuals clinically diagnosed with PD and those without synucleinopathies (control group). Brain biopsy samples were obtained from 14 PD patients and 6 controls without synucleinopathies. Additionally, skin samples were collected from 214 PD patients and 208 control subjects. Data were analyzed from April 2019 to May 2023. Results QSAA successfully amplified αSyn aggregates in brain tissue sections from mice inoculated with pre-formed fibrils. In the skin samples from 214 PD cases and 208 non-PD cases, QSAA demonstrated high sensitivity (90.2%) and specificity (91.4%) in differentiating between PD and non-PD cases. Notably, more αSyn aggregates were detected by QSAA compared to immunofluorescence with the pS129-αSyn antibody in consecutive slices of both brain and skin samples. Conclusion We introduced the new QSAA method tailored for in situ amplification of αSyn aggregates in brain and skin samples while maintaining tissue integrity, providing a streamlined approach to diagnosing PD with individual variability. The integration of seeding activities with the location of deposition of αSyn seeds advances our understanding of the mechanism underlying αSyn misfolding in PD.

To investigate the role of the HvnAnt2 gene in the formation of different qingke barley grain colours, HvnAnt2 was isolated from the leaves of the White 91-97-3 (white), Blue qingke (blue), Kunlun 17 (black), and Purple qingke (purple). The HvnAnt2 gene encodes a hydrophilic unstable protein consisting of 561 amino acids, without a transmembrane structure and without a signal peptide, with one bHLH-MYC_N and one HLH domain at amino acids 22-204 and 387-436, respectively. The HvnAnt2 of qingke barley was most closely related to Hordeum vulgare and most distantly related to Triticum monococcum; it was mainly related to light responsiveness and methyl jasmonate responsiveness. The Ant2 gene was highly expressed in Kunlun 17 and Purple qingke barley grains during the later stage of development. The protein interaction prediction showed that flavonoid 3'-monooxygenase, anthocyanin biosynthesis gene regulators, and key enzymes in folate metabolism interacted with the Ant2 protein. This study provides a reference for further analysis of the Ant2 gene in the anthocyanin synthesis pathways of qingke barley with different grain colours.

Abstract The seeding amplification assay (SAA) has recently emerged as a valuable tool for detecting α-synuclein (αSyn) aggregates in various clinically accessible biospecimens. Despite its efficiency and specificity, optimal tissue-specific conditions for distinguishing Parkinson’s disease (PD) from non-PD outside the brain remain underexplored. This study systematically evaluated 150 reaction conditions to identify the one with the highest discriminatory potential between PD and non-synucleinopathy controls using skin samples, resulting in a modified SAA. The streamlined SAA achieved an overall sensitivity of 92.46% and specificity of 93.33% on biopsy skin samples from 332 PD patients and 285 controls within 24 h. Inter-laboratory reproducibility demonstrated a Cohen’s kappa value of 0.87 (95% CI 0.69–1.00), indicating nearly perfect agreement. Additionally, αSyn seeds in the skin were stable at −80 °C but were vulnerable to short-term exposure to non-ultra-low temperatures and grinding. This study thoroughly investigated procedures for sample preprocessing, seed amplification, and storage, introducing a well-structured experimental framework for PD diagnosis using skin samples.

A physical mechanics simulation experiment is one of the important technical methods to study the inoculation mechanism and development laws of coal and gas outburst. A new type of coal and gas outburst simulation system has been developed, aiming at developing an advanced experimental design scheme with the help of existing advanced monitoring technology, combined with specific gas geology conditions, to realize the simulation of coal and gas outburst phenomenon in complex scenes, and to promote the study of coal and gas outburst mechanism. The physical mechanics simulation system consists of following seven parts: stress loading subsystem, test chamber subsystem, power traction subsystem, gas pressure control subsystem, induced equipment subsystem, simulated roadway subsystem, and measurement and control subsystem. The design of each subsystem has been improved to different degrees, and the whole system has formed a landmark innovation. The simulation system has the following characteristics: ① the high load loading and unloading function of the three-axis synchronous continuous non-uniform loading technical equipment is improved. The specimen loading is done by 10 independent indenters in X, Y, and Z directions. The size of the experimental chamber is 400 mm × 400 mm × 1200 mm, the maximum load strength is 25 MPa, and the maximum closed gas pressure is 6.0 MPa. Each of the above 10 indenters can operate asynchronously or synchronously for loading or unloading tasks according to instructions, and horizontal stress loading and unloading layered processing function is implemented. ② An integrated process of systematic sample preparation, sample packing, sealing, and loading has been formed completely. The experimental chamber is designed as a new type of split structure, and the dynamic traction system is used to assist the experimental chamber to complete the molding, stress loading and material handling, and the translation amount of the chamber structure is controlled accurately. ③ The four kinds of technologies for inducing coal and gas outburst are integrated and applied in the same simulation platform, and some equipment functional innovations are realized, such as the test chamber, the pressure relief window, etc. Many kinds of technology and equipment have been successfully developed, as original innovation products, the dynamic technology and the drilling equipment have been verified by the experimental simulation. ④ The design innovation of key device structure is realized. The problems of stress concentration and high pressure gas leakage under synchronous loading are solved by the design of double-layer steel plate chamber structure. The experimental efficiency is improved by the structural optimization of the launching cavity, the safety of the simulation system is significantly improved by the design of the pipe end structure, a combination of soft and hard material pipe structure, and the variable diameter drilling technology has achieved the engineering application of the Rouleauxs triangle theory. ⑤ Simulation test precision is generated by multivariate information. Advanced data acquisition systems (stress, gas pressure, temperature, etc.) and monitoring technology equipment including acoustic emission and high-speed cameras are used to achieve multi-data monitoring of coal and gas outburst. ⑥ The reliability of the experimental system has been verified by laboratory simulation. Through the case simulation of the “3·22 accident in Pingdingshan”, the overall test of the system function is completed, and the influence law of fault structure on in-situ stress, gas pressure and temperature in the process of coal and gas outburst is discovered in a new way. It is proved that the physical mechanics simulation system is reliable and could provide a platform to support the research on the coal and gas outburst mechanism.

Citrus Huanglongbing (HLB) is one of the most serious citrus diseases worldwide, causing huge economic losses every year and seriously affecting the development of the global citrus industry. In Lingnan region of China, HLB threatens the growth of important medicinal plants, such as Citrus reticulata 'Chachi', C. grandis 'Tomentosa', and C. medica 'Fingered'. Candidatus Liberibacter asiaticus (CLas) is the most common pathogen, which colonizes the phloem of plants and mainly infects rutaceae citrus species. At present, there is a lack of reports on the successful cultivation of CLas in vitro, making it difficult to study the pathogenic mechanism and control of the disease. Effector (also called effector protein) is a kind of pathogenic factor secreted by pathogens to the host, serving as an important weapon for pathogens to infect plants. The effectors are capable of targeting the key immune signal transduction and regulatory components of host cells, thereby inhibiting plant defense responses and enhancing the pathogenicity of pathogens. CLas can secrete over a hundred effectors, mainly divided into Secdependent effectors and non-classical secretory effectors according to different secretion pathways. Among them, there are 90 Sec-dependent effectors and 15 non-classical secretory effectors. They play a key role in the pathogenic process of infecting the host. Studies have shown that HLB effectors could target multiple cellular processes including plant immune signal transduction, autophagy, salicylic acid signaling pathway, and seriously disrupt the host's defense response to achieve colonization. Combined with recent researches on the pathogenic mechanism of HLB effectors, this article elaborates on the research overview of HLB and the classification of HLB effectors, and summarizes the pathogenic mechanism of effectors that affect citrus defense response. Finally, it offers a prospect on the future research directions, discusses the possibility of searching for important resistance genes to HLB based on identification of effector targets, with a aim to provide guidance for the pathogenic mechanism and disease control of HLB through the study of the effectors.

Abstract Background Cor triatriatum sinister (CTS) is an uncommon congenital cardiac anomaly. Atrial fibrillation (AF) is commonly the initial symptom in patients with CTS, occurring in approximately 32% of the cases. The complexity of performing AF catheter ablation, particularly in cases with persistent AF, increases in patients with CTS due to its unique structural challenges. Case presentation We report the treatment course of a 60-year-old male patient diagnosed with CTS, who underwent catheter ablation of drug-refractory, persistent AF. The complex anatomical structure of the condition made catheter ablation of AF challenging. To navigate these challenges, we performed comprehensive assessments using transthoracic echocardiography and transesophageal echocardiography, along with cardiac computed tomography angiography, prior to treatment initiation. The intricate anatomy of CTS was further clarified during the procedure via intracardiac echocardiography (ICE). Additionally, the complexity of catheter manipulation was further reduced with the aid of the VIZIGO sheath and the vein of Marshall ethanol infusion to achieve effective mitral isthmus blockage, thereby circumventing the impact of the CTS membrane. Conclusions This case underscores the complexity and potential of advanced ablation techniques in managing cardiac arrhythmias associated with unusual cardiac anatomies. During the procedure, ICE facilitated detailed modeling of the left atrium, including the membranous structure and its openings, thus providing a clearer understanding of CTS. It is noteworthy that the membrane within the CTS may serve as a potential substrate for arrhythmias, which warrants further validation through larger sample studies.

Abstract The biodegradable polymer poly-β-hydroxybutyrate (PHB) is a promising carbon source for biological mitigation of nitrogen pollution, a significant problem in aquaculture that physical and chemical methods have not provided a comprehensive solution. Here we investigated the impact of PHB on the zero-water-change largemouth bass culture by 30- and 40-day experiments. PHB loaded into the filter circulation pump at 4 g L− 1, optimum value determined by the first experiment, significantly reduced the levels of nitrate by 99.65%, nitrite by 95.96%, and total nitrogen by 85.22% compared to the control without PHB. PHB also significantly increased denitrifying bacteria (e.g., Proteobacteria and Fusobacteria) and expression of denitrification genes (e.g., nirK and nirS) in the microbial community, improving growth and health parameters of largemouth bass. While the impact may vary in other culture systems, PHB thus demonstrated its remarkable utility in aquaculture, highlighting ecological assessment and application to larger aquaculture operations as future considerations.

Abstract Background Existing researches on nurses’ work engagement and job burnout have mostly stayed at the individual level, and limited researches test the cross-level effects of psychosocial safety climate (PSC). The study aimed to explore the cross-level mediating effect of organization-based self-esteem (OBSE) and the moderating effect of psychological detachment between the relationship of PSC and work engagement and job burnout in nurses. Methods The cross-sectional study was conducted during November to December 2022 at a tertiary hospital in a northeastern province of China. Data was collected from 1832 nurses through an online questionnaire. Correlation analyses and hierarchical linear modeling were used to test study hypotheses. Results The results showed that PSC was positively associated with work engagement, and negatively associated with job burnout. OBSE mediated the effect of PSC on work engagement, as well as job burnout. Additionally, psychological detachment played a moderating role between PSC and work engagement, but no moderating effect was found between PSC and job burnout. Conclusions PSC at the organizational level increases work engagement and reduces job burnout by stimulating nurses’ high levels of OBSE. Psychological detachment, as a situational factor, enhances the positive influence of PSC on work engagement. The implementation of measures to improve the PSC levels of the organization, and the levels of OBSE and psychological detachment among nurses could help to promote their good work performance.

Abstract Purpose In this study, the actor–partner interdependence mediation model (APIMeM) was applied to breast cancer patients and their caregivers to assess the factors that affect the fear of cancer recurrence. In particular, the purpose of this study was to evaluate the mediating effect of social support on financial toxicity and the fear of cancer recurrence, providing an effective basis for developing plans to reduce the level of fear of cancer recurrence. Methods This study employed a cross-sectional design, and 405 dyads of breast cancer patients and their caregivers were enrolled. Financial toxicity, social support, and fear of cancer recurrence were assessed by computing comprehensive scores for financial toxicity based on patient-reported outcome measures, the Social Support Rating Scale, and the Fear of Cancer Recurrence Inventory Short Form, respectively. The data were analysed using SPSS 24.0 and AMOS 23.0. Results The results showed that the fear of cancer recurrence of breast cancer patients and their caregivers was significantly related to dyadic financial toxicity and social support. In addition, the financial toxicity of breast cancer patients and their caregivers had significant actor effects and partner effects on the fear of cancer recurrence through dyadic social support. Conclusions The financial toxicity of breast cancer patients and their caregivers could produce actor and partner effects on the fear of cancer recurrence through the mediation of social support, which provided empirical support for improving reducing the level of fear of cancer recurrence among patients and caregivers at the dyadic level.

Abstract Broad bean (Vicia faba L.) has received attention from the food and animal feed industries as a sustainable, inexpensive, and versatile plant‐based protein source. However, long‐term consumption of broad bean, especially unprocessed ones, can lead to hepatic lipid accumulation. To investigate how broad bean affected hepatic lipid metabolism and its underlying mechanisms, we conducted a multi‐omics analysis of crisp grass carp (Ctenopharyngodon idellus C. et V) model with hepatic steatosis after feeding with single broad bean for 120 days. The glycolysis inhibition, increased de novo lipogenesis, abnormalities in lipid metabolism, and activation of oxidative stress were the key processes involved in broad bean‐induced hepatic lipid accumulation. Disturbances in intestinal flora caused by damage to the intestinal barrier and increased lipopolysaccharide caused by broad bean promoted hepatic lipid accumulation. Reduction of antinutritional factors and balanced diet offer potential strategies to mitigate the liver damage caused by broad bean consumption. Overall, our findings provided a theoretical basis for the wide application of broad bean as animal feeds.

Abstract Parkinson’s disease is a progressive neurodegenerative disorder in which loss of dopaminergic neurons in the substantia nigra results in a clinically heterogeneous group with variable motor and non-motor symptoms with a degree of misdiagnosis. Only 3–25% of sporadic Parkinson’s patients present with genetic abnormalities that could represent a risk factor, thus environmental, metabolic, and other unknown causes contribute to the pathogenesis of Parkinson’s disease, which highlights the critical need for biomarkers. In the present study, we prospectively collected and analyzed plasma samples from 194 Parkinson’s disease patients and 197 age-matched non-diseased controls. N-acetyl putrescine (NAP) in combination with sense of smell (B-SIT), depression/anxiety (HADS), and acting out dreams (RBD1Q) clinical measurements demonstrated combined diagnostic utility. NAP was increased by 28% in Parkinsons disease patients and exhibited an AUC of 0.72 as well as an OR of 4.79. The clinical and NAP panel demonstrated an area under the curve, AUC = 0.9 and an OR of 20.4. The assessed diagnostic panel demonstrates combinatorial utility in diagnosing Parkinson’s disease, allowing for an integrated interpretation of disease pathophysiology and highlighting the use of multi-tiered panels in neurological disease diagnosis.

Presently, integrating multi-omics information into a prediction model has become a ameliorate strategy for genomic selection to improve genomic prediction accuracy. Here, we set the genomic and transcriptomic data as the training population data, using BSLMM, TWAS, and eQTL mapping to prescreen features according to | ^βb|>0, top 1% of phenotypic variation explained (PVE), expression-associated single nucleotide polymorphisms (eSNPs), and egenes (false discovery rate (FDR)

Abstract Gliomas are highly heterogenous diseases with poor prognosis. Precise survival prediction could benefit further clinical decision‐making, clinical trial incursion, and health economics. Recent research has emphasized the prognostic value of magnetic resonance imaging, pathological specimens, and circulating biomarkers. However, the integrative potential and efficacy of these modalities require to be further validated. After incorporating 218 patients of The Cancer Genome Atlas glioma datasets of and 54 patients of the Huashan cohort with complementary prognostic information, we established a squeeze‐and‐excitation deep learning feature extractor for T1‐contrast enhanced images and histological slides and explored to screen significant circulating 5‐hydroxymethylcytosines (5hmC) profiles for glioma survival by least absolute shrinkage and selection operator‐Cox regression. Therefore, a prognostication predictive model with high efficiency was obtained through survival support vector machine multimodal integration of radiologic imaging, histopathologic imaging features, genome‐wide 5hmC in circulating cell‐free DNA and clinical variables, suggesting a valid strategy (concordance‐index = 0.897; Brier score = 0.118) for improved survival risk stratification of glioma patients.

IntroductionInflammatory bowel disease (IBD) is a multi-organ autoimmune disease that commonly affects the gastrointestinal tract, but can also affect other organs throughout the body. Less is known, however, about kidney involvement in IBD. Although IBD has been associated with chronic kidney disease (CKD) and end-stage renal disease (ESRD), these results have been inconsistent. The present study analyzed the prevalence of concurrent CKD and ESRD in patients with IBD.MethodsPubMed, Cochrane, Web of Science, and Embase were searched for studies published through October 2023 on IBD patients with concurrent CKD or ESRD. Outcomes included the incidence rates and odds ratios (OR) of concurrent CKD and ESRD in IBD patients. The quality of included studies was assessed using the Newcastle-Ottawa Scale, and sequential sensitivity was analyzed. Publication bias was evaluated using Egger’s test.ResultsNine studies were included in this meta-analysis. The combined results of eight studies, which included 239,042 IBD patients, showed that the prevalence of CKD in IBD patients was 5% (95% confidence interval [CI]: 1–9%). The combined results of two studies, which included 40,341 IBD patients, showed that the prevalence of ESRD in IBD patients was 0.2% (95% CI: −0.08–0.12%). The combined results of six case–control studies reported that the risk of CKD was significantly higher in patients with than without IBD (OR 1.36, 95% CI: 1.08–1.70, p = 0.008).ConclusionAlthough studies have shown an increased risk of CKD in IBD, due to the small number of included studies and high heterogeneity across studies, it is not enough to definitively conclude that CKD is more common in patients with IBD. But patients with IBD should be regularly monitored for CKD.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/.

The pregnane X receptor (PXR)/nuclear factor-kB (NF-kB) signaling pathway plays a critical role in regulating toxin-induced inflammation and apoptosis in mammals. Whether dietary curcumin (CUR) can prevent copper (Cu)-induced liver injury via this signaling pathway remains to be established in aquatic animals. Juvenile largemouth bass (Micropterus salmoides) were exposed to dietary Cu and CUR treatments for 8 weeks. The results showed that chronic Cu exposure induced oxidative stress, causing liver function damage and liver injury. Cu exposure stimulated inflammation by regulating nf-kb and pro-inflammatory genes such as tnfα and il-1β and promoted apoptotic signals in the liver by modulating bcl2 and casp3 mRNA levels. In addition, Pearson correlation analysis verified that inflammatory and apoptotic responses were important indicators of Cu-induced liver injury. CUR attenuated stress responses by enhancing the antioxidant system. Importantly, CUR significantly stimulated PXR mRNA and protein levels in the Cu + CUR group and suppressed NF-κB activation to inhibit the inflammatory and apoptotic signaling cascade. These results suggest that CUR may be an effective activator of PXR in teleost fishes, exerting cytoprotective effects on Cu-induced liver injury via a PXR-mediated NF-κB repression mechanism. In conclusion, this study is the first to demonstrate that CUR may act as a potent PXR ligand that exerts hepatoprotective effects against Cu-induced liver injury. The findings shed light on the specific regulatory role of the PXR/NF-κB signaling pathway in liver pathogenesis and its potential as a therapeutic target in teleost fishes.

Substantial evidence indicate that Antarctic krill oil (KO) is an effective method for alleviating acute ulcerative colitis (UC). Nevertheless, UC is a chronic disease, and the chronic colitis model is more conducive to simulating its pathological characteristics. The objective of this study was to establish a chronic UC model in C57BL/6 mice and identify the regulatory consequences and feasible mechanisms of KO on chronic UC and its associated depressive-like behaviors. The results showed that KO alleviated the pathological features of UC and depression-like behavior. Further mechanisms suggested that KO significantly suppressed gut-brain inflammation, increased the concentration of 5-hydroxytryptamine (5-HT) and the expression of postsynaptic density protein 95 (PSD95) and brain-derived neurotrophic factor (BDNF), protected the integrity of the gut barrier and blood–brain barrier. Moreover, KO maintained the equilibrium of the intestinal flora. The findings suggested that KO may be a promising approach for alleviating UC and its concomitant psychiatric disorders.

Ischemic stroke poses a significant global health challenge with limited treatment options. Tissue plasminogen activator, the only effective medication, has strict restrictions, limiting its benefits only to a small number of patients. Astaxanthin, a natural carotenoid found in algae, shrimp, and crabs, has demonstrated promising neuroprotective properties in models of ischemic stroke. This article reviews the novel finding of neuroprotective impact of astaxanthin in ischemic stroke, highlighting its benefits in various protective mechanisms such as antioxidation, anti-inflammation, enhancement of DNA repair, anti-cell death, protection of blood–brain barrier, and promotion of neuronal survival. This analysis underscores the therapeutic and preventive potential of astaxanthin in ischemic stroke, positioning it as a prospective pharmaceutical agent against ischemic stroke.

Given the substantial initial investment required by the drilling and implementation of the Deep Borehole Heat Exchanger (DBHE), it becomes imperative to quantitatively evaluate its long-term performance and sustainability. This work introduces a pilot DBHE project in Xi’an, along with the 500-h in-situ monitored data, which is used to validate the 3D numerical model established and simulated in the OpenGeoSys (OGS) software. Based on the validated model, a series of extended scenarios are executed to evaluate the influence of design and operational parameters on the long-term performance and sustainability of the DBHE system. The results show that the drilling depth is the most significant factor that influences long-term performance. On the contrary, pipe diameter and inner pipe thermal conductivity have a very limited impact. A comprehensive evaluation is suggested to determine operational flow rates in real projects while considering the energy consumption by the circulation pump. Moreover, the heat extraction performance and energy analysis of the DBHE coupled with a geothermal heat pump under intermittent operation are also investigated. With a fixed daily heating demand, a longer daily operating time results in lower circulating temperature drops, which is advantageous for the heat pump’s energy efficiency in long-term operation.

ObjectivesMusculoskeletal pain after COVID-19 infection remains a concerning long-term complication of COVID-19. Here, our study aimed to investigate the prevalence of musculoskeletal pain associated with COVID-19 (MSPC) and healthcare-seeking behaviors, as well as the associating factors.MethodsA cross-sectional survey was conducted using convenience sampling and distributed to participants anonymously through the online platform Credamo. Demographic and characteristic data of the participants were collected and analyzed. Logistic regression analysis was employed to investigate potential factors associated with MSPC and healthcare-seeking tendencies.ResultsA total of 1,510 participants responded to the survey, with 42.6% (643 individuals) exhibiting MSPC. Higher education level and a greater number of concomitant symptoms were significant risk factors for MSPC, while longer exercise duration and higher PSS-10 scores were protective factors. Additionally, higher income level, frequency and severity of pain, and greater PSS-10 scores increased healthcare-seeking intention.ConclusionA significant proportion of individuals experience MSPC. Education level and concomitant symptoms were risk factors for MSPC, while exercise duration and PSS-10 score were potential protective factors. Income level, frequency and severity of pain, and PSS-10 score are significantly related to the willingness to seek medical treatment for MSPC.

Background and aimsInflammatory bowel disease (IBD) is a common chronic inflammatory bowel disease characterized by diarrhea and abdominal pain. Recently human metabolites have been found to help explain the underlying biological mechanisms of diseases of the intestinal system, so we aimed to assess the causal relationship between human blood metabolites and susceptibility to IBD subtypes.MethodsWe selected a genome-wide association study (GWAS) of 275 metabolites as the exposure factor, and the GWAS dataset of 10 IBD subtypes as the outcome, followed by univariate and multivariate analyses using a two-sample Mendelian randomization study (MR) to study the causal relationship between exposure and outcome, respectively. A series of sensitivity analyses were also performed to ensure the robustness of the results.ResultsA total of 107 metabolites were found to be causally associated on univariate analysis after correcting for false discovery rate (FDR), and a total of 9 metabolites were found to be significantly causally associated on subsequent multivariate and sensitivity analyses. In addition we found causal associations between 7 metabolite pathways and 6 IBD subtypes.ConclusionOur study confirms that blood metabolites and certain metabolic pathways are causally associated with the development of IBD subtypes and their parenteral manifestations. The exploration of the mechanisms of novel blood metabolites on IBD may provide new therapeutic ideas for IBD patients.

Abstract Bietti crystalline corneoretinal dystrophy is an inherited retinal disease caused by mutations in CYP4V2, which results in blindness in the working-age population, and there is currently no available treatment. Here, we report the results of the first-in-human clinical trial (NCT04722107) of gene therapy for Bietti crystalline corneoretinal dystrophy, including 12 participants who were followed up for 180–365 days. This open-label, single-arm exploratory trial aimed to assess the safety and efficacy of a recombinant adeno-associated-virus-serotype-2/8 vector encoding the human CYP4V2 protein (rAAV2/8-hCYP4V2). Participants received a single unilateral subretinal injection of 7.5 × 1010 vector genomes of rAAV2/8-hCYP4V2. Overall, 73 treatment-emergent adverse events were reported, with the majority (98.6%) being of mild or moderate intensity and considered to be procedure- or corticosteroid-related; no treatment-related serious adverse events or local/systemic immune toxicities were observed. Compared with that measured at baseline, 77.8% of the treated eyes showed improvement in best-corrected visual acuity (BCVA) on day 180, with a mean ± standard deviation increase of 9.0 ± 10.8 letters in the 9 eyes analyzed (p = 0.021). By day 365, 80% of the treated eyes showed an increase in BCVA, with a mean increase of 11.0 ± 10.6 letters in the 5 eyes assessed (p = 0.125). Importantly, the patients’ improvement observed using multifocal electroretinogram, microperimetry, and Visual Function Questionnaire-25 further supported the beneficial effects of the treatment. We conclude that the favorable safety profile and visual improvements identified in this trial encourage the continued development of rAAV2/8-hCYP4V2 (named ZVS101e).

Abstract Background Ovarian damage and follicle loss are major side effects of chemotherapy in young female patients with cancer. However, effective strategies to prevent these injuries are still lacking. The purpose of this study was to verify low-intensity pulsed ultrasound (LIPUS) can reduce ovarian injury caused by chemotherapy and to explore its underlying mechanisms in mice model. Methods The mice were randomly divided into the Control group, Cisplatin group, and Cisplatin + LIPUS group. The Cisplatin group and Cisplatin + LIPUS group were intraperitoneally injected with cisplatin every other day for a total of 10 injections, and the Control group was injected with saline. On the second day of each injection, the Cisplatin + LIPUS group received irradiation, whereas the other two groups received sham irradiation. We used a variety of biotechnologies to detect the differences in follicle count, granulosa cell apoptosis, fibrosis, transcriptome level, oxidative damage, and inflammation in differently treated mice. Result LIPUS was able to reduce primordial follicle pool depletion induced by cisplatin and inhibit the apoptosis of granulosa cells. Transcriptomic results confirmed that LIPUS can reduce ovarian tissue injury. We demonstrated that LIPUS can relieve ovarian fibrosis by inhibiting TGF-β1/Smads pathway. Meanwhile, it can reduce the oxidative damage and reduced the mRNA levels of proinflammatory cytokines caused by chemotherapy. Conclusion LIPUS can reduce the toxic effects of chemotherapy drugs on ovaries, inhibit ovarian fibrosis, reduce the inflammatory response, and redcue the oxidative damage, reduce follicle depletion and to maintain the number of follicle pools.

Abstract Background The genus Caragana encompasses multiple plant species that possess medicinal and ecological value. However, some species of Caragana are quite similar in morphology, so identifying species in this genus based on their morphological characteristics is considerably complex. In our research, illumina paired-end sequencing was employed to investigate the genetic organization and structure of Caragana tibetica and Caragana turkestanica, including the previously published chloroplast genome sequence of 7 Caragana plants. Results The lengths of C. tibetica and C. turkestanica chloroplast genomes were 128,433 bp and 129,453 bp, respectively. The absence of inverted repeat sequences in these two species categorizes them under the inverted repeat loss clade (IRLC). They encode 110 and 111 genes (4 /4 rRNA genes, 30 /31tRNA genes, and 76 /76 protein-coding genes), respectively. Comparison of the chloroplast genomes of C. tibetica and C. turkestanica with 7 other Caragana species revealed a high overall sequence similarity. However, some divergence was observed between certain intergenic regions (matK-rbcL, psbD-psbM, atpA-psbI, and etc.). Nucleotide diversity (π) analysis revealed the detection of five highly likely variable regions, namely rps2-atpI, accD-psaI-ycf4, cemA-petA, psbN-psbH and rpoA-rps11. Phylogenetic analysis revealed that C. tibetica’s sister species is Caragana jubata, whereas C. turkestanica’s closest relative is Caragana arborescens. Conclusions The present study provides worthwhile information about the chloroplast genomes of C. tibetica and C. turkestanica, which aids in the identification and classification of Caragana species.

Abstract Assistive medical image classifiers can greatly reduce the workload of medical personnel. However, traditional machine learning methods require large amounts of well-labeled data and long learning times to solve medical image classification problems, which can lead to high training costs and poor applicability. To address this problem, a novel unsupervised breast cancer image classification model based on multiscale texture analysis and a dynamic learning strategy for mammograms is proposed in this paper. First, a gray-level cooccurrence matrix and Tamura coarseness are used to transfer images to multiscale texture feature vectors. Then, an unsupervised dynamic learning mechanism is used to classify these vectors. In the simulation experiments with a resolution of 40 pixels, the accuracy, precision, F1-score and AUC of the proposed method reach 91.500%, 92.780%, 91.370%, and 91.500%, respectively. The experimental results show that the proposed method can provide an effective reference for breast cancer diagnosis.

Abstract With the growth of the digital economy, the sustainable growth of rural energy has become crucial. However, traditional rural energy models have the drawback of not considering digital technology and renewable energy. Therefore, there is an urgent need for rational planning and development of rural energy. According to this, a multi-energy coupling model for rural energy systems was established by considering equipment capacity planning and operation scheduling optimization based on a multi-energy coupling structure. At the same time, considering the biomass resources in rural energy systems, an optimized configuration model for biomass coal-fired coupled power generation units was established. The results showed that the energy consumption cost in County A accounted for only 3.3%. County C focused mainly on tourism and emphasized economic efficiency, with investment costs 8.6% and 10.3% lower than other rural areas. The system utilized time of use electricity prices to optimize operation. The low storage stage was from 1:00 to 8:00, while the high incidence stage was from 12:00 to 14:00 and from 7:00 to 21:00. In the actual scenario, the multi-energy coupling model can be combined with intelligent technology to realize the real-time monitoring, prediction and optimal control of the energy system. Through the introduction of advanced digital technology, the model can be more flexible to deal with the diversified energy sources and complex operational scheduling situations involved in rural energy systems. This can improve the response speed and adaptability of the system, making the energy system more resilient and efficient.

Abstract Background Teriparatide (TPTD) is a widely used anabolic agent for the treatment of osteoporosis. Several factors have been identified to be related to bone mineral density (BMD) increase in anti-osteoporosis treatment with other agents; however, there has been no systematic analysis to summarize the associated determinants of BMD reaction to daily teriparatide treatment. Methods In this retrospective study, we performed a comprehensive investigation involving not only clinical data but also several relevant lifestyle factors to be examined for their potential contribution to BMD response. This post-hoc analysis included 258 post-menopaused patients with osteoporosis who received TPTD at 20 µg/day for 12 months. Univariate and multivariate analyses were conducted to distinguish the response variables of lumbar spine (LS) BMD transformation, the principal outcome measure of efficacy, from the baseline at 12 months. Results Twelve months of TPTD treatment resulted in an absolute 0.39 ± 0.37 increase in T-score of LS BMD. Gastrointestinal disease, prior bisphosphonate or glucocorticoid treatment, no vitamin K2 supplementation, low levels of serum 25(OH)D and PINP, weak increment of PINP and β-CTX at 3 months, unhealthy lifestyle (excessive smoking, tea, coffee, and drinking), vegetarian diet pattern, low ALT level, and high BMD at baseline were determined by univariate analyses to be related to the weak reaction of TPTD treatment (P

Abstract Background Whether Transanal drainage tubes (TDTs) placement reduces the occurrence of anastomotic leakage (AL) after rectal cancer (RC) surgery remains controversial. Most existing meta-analyses rely on retrospective studies, while the prospective studies present an inadequate level of evidence. Methods A systematic review and meta-analysis of prospective studies on TDTs placement in RC patients after surgery was conducted. The main analysis index was the incidence of AL, Grade B AL, and Grade C AL, while secondary analysis index was the incidence of anastomotic bleeding, incision infection, and anastomotic stenosis. A comprehensive literature search was performed utilizing the databases Cochrane Library, Embase, PubMed, and Web of Science. We recorded Risk ratios (RRs) and 95% confidence intervals (CI) for each included study, and a fixed-effect model or random-effect model was used to investigate the correlation between TDTs placement and four outcomes after RC surgery. Results Seven studies (1774 participants, TDT 890 vs non-TDT 884) were considered eligible for quantitative synthesis and meta-analysis. The meta-analysis revealed that the incidence of AL was 9.3% (83/890) in the TDT group and 10.2% (90/884) in the non-TDT group. These disparities were found to lack statistical significance (P = 0.58). A comprehensive meta-analysis, comprising four studies involving a cumulative sample size of 1259 participants, revealed no discernible disparity in the occurrence of Grade B AL or Grade C AL between the TDT group and the non-TDT group (Grade B AL: TDT 34/631 vs non-TDT 26/628, P = 0.30; Grade C AL: TDT 11/631 vs non-TDT 27/628, P = 0.30). Similarly, the incidences of anastomotic bleeding (4 studies, 876 participants), incision infection (3studies, 713 participants), and anastomotic stenosis (2studies, 561 participants) were 5.5% (24/440), 8.1% (29/360), and 2.9% (8/280), respectively, in the TDT group, and 3.0% (13/436), 6.5% (23/353), and 3.9% (11/281), respectively, in the non-TDT group. These differences were also determined to lack statistical significance (P = 0.08, P = 0.43, P = 0.48, respectively). Conclusion The placement of TDTs does not significantly affect the occurrence of AL, Grade B AL, and Grade C AL following surgery for rectal cancer. Additionally, TDTs placement does not be associated with increased complications such as anastomotic bleeding, incision infection, or anastomotic stenosis. Trial registration PROSPERO: CRD42023427914

Abstract Background Numerous species within the genus Caragana have high ecological and medicinal value. However, species identification based on morphological characteristics is quite complicated in the genus. To address this issue, we analyzed complete plastid genome data for the genus. Results We obtained chloroplast genomes of two species, Caragana arborescens and Caragana opulens, using Illumina sequencing technology, with lengths of 129,473 bp and 132,815 bp, respectively. The absence of inverted repeat sequences in the two species indicated that they could be assigned to the inverted repeat-lacking clade (IRLC). The genomes included 111 distinct genes (4 rRNA genes, 31 tRNA genes, and 76 protein-coding genes). In addition, 16 genes containing introns were identified in the two genomes, the majority of which contained a single intron. Repeat analyses revealed 129 and 229 repeats in C. arborescens and C. opulens, respectively. C. arborescens and C. opulens genomes contained 277 and 265 simple sequence repeats, respectively. The two Caragana species exhibited similar codon usage patterns. rpl20-clpP, rps19-rpl2, and rpl23-ycf2 showed the highest nucleotide diversity (pi). In an analysis of sequence divergence, certain intergenic regions (matK-rbcL, psbM-petN, atpA-psbI, petA-psbL, psbE-petL, and rps7-rps12) were highly variable. A phylogenetic analysis showed that C. arborescens and C. opulens were related and clustered together with four other Caragana species. The genera Astragalus and Caragana were relatively closely related. Conclusions The present study provides valuable information about the chloroplast genomes of C. arborescens and C. opulens and lays a foundation for future phylogenetic research and molecular marker development.

Background Idiopathic Pulmonary Fibrosis (IPF) presents a severe respiratory challenge with a poor prognosis due to the lack of reliable biomarkers. Recent evidence suggests that Endoplasmic Reticulum Stress (ERS) may be associated with IPF pathogenesis. This study focuses on uncovering ERS-associated biomarkers for IPF.Methods Sequencing data from diverse datasets were analyzed, utilizing differential gene expression analysis and Weighted Gene Co-expression Network Analysis (WGCNA). Endoplasmic Reticulum Stress (ERS)-related genes were extracted from the GeneCards database. Hub genes were identified through Protein-Protein Interaction (PPI) analysis. Diagnostic and prognostic models were developed using machine learning algorithms and validated across both training and validation sets. Additionally, techniques such as Cell-type Identification by Estimating Relative Subsets of RNA Transcripts and single-cell RNA sequencing were employed to identify potential IPF-related cells. These findings were further investigated to elucidate their underlying mechanisms through in vitro experiments.Results Differentially expressed genes, WGCNA-identified blue module genes, and ERS-related genes extracted from the GeneCards database were intersected, and the resulting genes were used to construct diagnostic and prognostic models. Validation using multiple datasets indicated that both the diagnostic and prognostic models possess strong predictive capabilities. PPI analysis highlighted SPP1 as a potential hub gene in IPF. Moreover, M2 macrophages were found in higher quantities in the lung tissue of IPF patients, with a significant increase in SPP1-expressing M2 macrophages compared to the control group. In vitro experiments demonstrated that exogenous SPP1 inhibited the proliferation and migration of M2 macrophages and promoted apoptosis within a certain concentration range.Conclusion This study identifies ERS-related biomarkers in IPF, highlighting SPP1 and M2 macrophages. The resulting diagnostic and prognostic models offer strong predictive capabilities, unveiling new therapeutic avenues.

Zirconia is a ceramic material with fine properties for dental restorations, but the color of zirconia does not match natural teeth color. In this study, the effect of zirconia doped with different rare earth oxides on the color of denture manufactured by Digital Light Processing (DLP) method was investigated. Experimental results showed that the color of the ceramic parts after sintering could cover Vita 3D colorimetric plate for natural teeth, and the color accuracy was within 1.5 NBS. Meanwhile, the forming accuracy of the denture reached 67.7 μm, and the three-point bending strength was 520.32 ± 35.44–649.10 ± 42.31 MPa, which met the requirements of triple crown in dentistry. This study establishes a method to achieve colors of natural teeth using DLP technology, and the results of this study have important leading implications for the use of additive manufacturing technology to fabricate natural-color ceramic dentures for dental applications.

Depression is a prevalent mental disorder that significantly diminishes quality of life and longevity, ranking as one of the primary causes of disability globally. Contemporary research has explored the potential pathogenesis of depression from various angles, encompassing genetics, neurotransmitter systems, neurotrophic factors, the hypothalamic-pituitary-adrenal axis, inflammation, and intestinal flora, among other contributing factors. In addition, conventional chemical medications are plagued by delayed onset of action, persistent adverse effects, and restricted therapeutic efficacy. In light of these limitations, the therapeutic approach of traditional Chinese medicine (TCM) has gained increasing recognition for its superior effectiveness. Numerous pharmacological and clinical studies have substantiated TCM’s capacity to mitigate depressive symptoms through diverse mechanisms. This article attempts to summarize the mechanisms involved in the pathogenesis of depression and to describe the characteristics of herbal medicines (including compounded formulas and active ingredients) for the treatment of depression. It further evaluates their effectiveness by correlating with the multifaceted pathogenesis of depression, thereby furnishing a reference for future research endeavors.

Due to the similarity and diversity among kinases, small molecule kinase inhibitors (SMKIs) often display multi-target effects or selectivity, which have a strong correlation with the efficacy and safety of these inhibitors. However, due to the limited number of well-known popular databases and their restricted data mining capabilities, along with the significant scarcity of databases focusing on the pharmacological similarity and diversity of SMIKIs, researchers find it challenging to quickly access relevant information. The KLIFS database is representative of specialized application databases in the field, focusing on kinase structure and co-crystallised kinase-ligand interactions, whereas the KLSD database in this paper emphasizes the analysis of SMKIs among all reported kinase targets. To solve the current problem of the lack of professional application databases in kinase research and to provide centralized, standardized, reliable and efficient data resources for kinase researchers, this paper proposes a research program based on the ChEMBL database. It focuses on kinase ligands activities comparisons. This scheme extracts kinase data and standardizes and normalizes them, then performs kinase target difference analysis to achieve kinase activity threshold judgement. It then constructs a specialized and personalized kinase database platform, adopts the front-end and back-end separation technology of SpringBoot architecture, constructs an extensible WEB application, handles the storage, retrieval and analysis of the data, ultimately realizing data visualization and interaction. This study aims to develop a kinase database platform to collect, organize, and provide standardized data related to kinases. By offering essential resources and tools, it supports kinase research and drug development, thereby advancing scientific research and innovation in kinase-related fields. It is freely accessible at: http://ai.njucm.edu.cn:8080.

Lung cancer persistently leads as the primary cause of morbidity and mortality among malignancies. A notable increase in the prevalence of lung adenocarcinoma has become evident in recent years. Although targeted therapies have shown in treating certain subsets of non-small cell lung cancers (NSCLC), a significant proportion of patients still face suboptimal therapeutic outcomes. Neuregulin-1 (NRG1), a critical member of the NRG gene family, initially drew interest due to its distribution within the nascent ventricular endocardium, showcasing an exclusive presence in the endocardium and myocardial microvessels. Recent research has highlighted NRG1’s pivotal role in the genesis and progression across a spectrum of tumors, influencing molecular perturbations across various tumor-associated signaling pathways. This review provides a concise overview of NRG1, including its expression patterns, configuration, and fusion partners. Additionally, we explore the unique features and potential therapeutic strategies for NRG1 fusion-positive occurrences within the context of NSCLC.

Habitual dietary changes have the potential to induce alterations in the host’s gut microbiota. Mandarin fish (Siniperca chuatsi), an aquatic vertebrate species with distinct feeding habits, were fed with natural feeds (NF) and artificial feeds (AF) to simulate the effects of natural and processed food consumption on host gut microbiota assemblages. The results showed that the alpha diversity index was reduced in the AF diet treatment, as lower abundance and diversity of the gut microbiota were observed, which could be attributed to the colonized microorganisms of the diet itself and the incorporation of plant-derived proteins or carbohydrates. The β-diversity analysis indicated that the two dietary treatments were associated with distinct bacterial communities. The AF diet had a significantly higher abundance of Bacteroidota and a lower abundance of Actinomycetota, Acidobacteriota, and Chloroflexota compared to the NF group. In addition, Bacteroidota was the biomarker in the gut of mandarin fish from the AF treatment, while Acidobacteriota was distinguished in the NF treatments. Additionally, the increased abundance of Bacteroidota in the AF diet group contributed to the improved fermentation and nutrient assimilation, as supported by the metabolic functional prediction and transcriptome verification. Overall, the present work used the mandarin fish as a vertebrate model to uncover the effects of habitual dietary changes on the evolution of the host microbiota, which may provide potential insights for the substitution of natural foods by processed foods in mammals.

BackgroundParkinson’s disease (PD), the second most prevalent neurodegenerative condition, has a multifaceted etiology. Cathepsin-cysteine proteases situated within lysosomes participate in a range of physiological and pathological processes, including the degradation of harmful proteins. Prior research has pointed towards a potential link between cathepsins and PD; however, the precise causal relationship between the cathepsin family and PD remains unclear.MethodsThis study employed univariate and multivariate Mendelian randomization (MR) analyses to explore the causal relationship between the nine cathepsins and Parkinson’s disease (PD) risk. For the primary analysis, genome-wide association study (GWAS) summary statistics for the plasma levels of the nine cathepsins and PD was obtained from the INTERVAL study and the International Parkinson’s Disease Genomics Consortium. GWAS for PD replication analysis were obtained from the FinnGen consortium, and a meta-analysis was performed for the primary and replication analyses to evaluate the association between genetically predicted cathepsin plasma levels and PD risk. After identifying significant MR estimates, genetic co-localization analyses were conducted to determine whether shared or distinct causal variants influenced both cathepsins and PD.ResultsElevated cathepsin B levels were associated with a decreased risk of PD in univariate MR analysis (odds ratio [OR] = 0.890, 95% confidence interval [CI]: 0.831–0.954, pFDR = 0.009). However, there was no indication that PD affected cathepsin B levels (OR = 0.965, 95% CI: 0.858–1.087, p = 0.852). In addition, after adjusting for the remaining cathepsins, cathepsin B levels independently and significantly contributed to the reduced risk of PD in multivariate MR analysis (OR = 0.887, 95% CI: 0.823–0.957, p = 0.002). The results of the replication MR analysis with the FinnGen GWAS for PD (OR = 0.921, 95% CI: 0.860–0.987, p = 0.020) and meta-analysis (OR = 0.905, 95% CI: 0.862–0.951, p

Mollisols rich in natural organic matter are a significant sink of carbon (C) and selenium (Se). Climate warming and agricultural expansion to the cold Mollisol regions may enhance soil respiration and biogeochemical cycles, posing a growing risk of soil C and Se loss. Through field-mimicking incubation experiments with uncultivated and cultivated soils from the Mollisol regions of northeastern China, this research shows that soil respiration remained significant even during cold seasons and caused co-emission of greenhouse gases (CO2 and CH4) and methylated Se. Such stimulus effects were generally stronger in the cultivated soils, with maximum emission rates of 7.45 g/m2/d C and 1.42 μg/m2/d Se. For all soil types, the greatest co-emission of CO2 and dimethyl selenide occurred at 25 % soil moisture, whereas measurable CH4 emission was observed at 40 % soil moisture with higher percentages of dimethyl diselenide volatilization. Molecular characterization with three-dimensional fluorescence and ultra-high resolution mass spectrometry suggests that CO2 emission is sensitive to the availability of microbial protein-like substances and free energy from organic carbon biodegradation under variable moisture conditions. Predominant Se binding to biodegradable organic matter resulted in high dependence of Se volatilization on rates of greenhouse gas emissions. These findings together highlight the importance of dynamic organic carbon quality for soil respiration and consequent Mollisol Se loss risk, with implications for science-based management of C and Se resources in agricultural lands to combat with Se deficiency.

Al–Mg–Li ternary alloy particles can be a promising metal additive to replace aluminum in solid propellants to improve combustion efficiency by reducing agglomeration during combustion. In this paper, Al–Mg–Li ternary alloy was prepared and its basic physical and chemical characterization parameters were obtained by SEM-EDS, ICP-AES, and XRD analysis. The ignition and combustion characteristics of single Al–Mg–Li particles under different pressures were investigated by using a self-developed experimental setup. The ignition process and combustion flame structure of Al–Mg–Li particles were significantly affected by the increasing pressure, and the ignition delay and combustion time decreased. The ignition mode was clearly identified: surface ignition (0.1–0.2 MPa) and gas phase ignition (0.4–1.2 MPa). Al–Mg–Li particles showed white-pink flame during combustion, and the characteristic peaks of AlO, MgO, and Li were identified, confirming that the particles performed a gas-phase combustion at pressures above 0.4 MPa. The combustion temperature of Al–Mg–Li particles could exceed 3000 °C when the pressure surpassed 0.6 MPa. The composition of different regions in the condensed combustion products were analyzed. Reaction mechanism was proposed to reveal the addition of elemental Mg and Li could improve the ignition and combustion characteristics of Al, especially at higher ambient pressures.

In our previous study, red kidney bean polysaccharides (RKP) have been regarded as the functional polysaccharide with glucolipid metabolism regulation activity. The impact of different concentrations of RKP (2, 4, and 6 g/100 g of dough weight) on the water mobility, rheology, and microstructure of frozen dough was examined using low field nuclear magnetic resonance (LF-NMR) and scanning electron microscopy (SEM) in the present study. The findings demonstrated that RKP exerted a positive impact on the water absorption capacity, moisture distribution, and viscoelastic properties of frozen dough. The addition of RKP significantly improved the water absorption of the dough. Specifically, on day 0, adding 6% RKP increased the water absorption rate from 25% to 47% compared to the blank group. Notably, the hardness of steamed bread decreased significantly with the addition of RKP, in addition, its springiness and resilience dependently increased. This offered a theoretical foundation for the use of RKP in the industrial manufacture of wheat-based foods.

BackgroundLimited studies have investigated the relationship between systemic oxidative stress and inflammatory bowel disease (IBD). The purpose of this study was to explore the relationship between oxidative balance score (OBS) and IBD.MethodsWe included 175,808 participants from the UK Biobank database from 2006 to 2010. OBS scores were calculated based on 22 lifestyle and dietary factors. Multiple variable Cox proportional regression models, as well as gender stratification and subgroup analysis, were utilized to investigate the relationship between OBS and IBD.ResultsThere is a significant negative correlation between OBS and the occurrence of IBD, ulcerative colitis (UC), and Crohn’s disease (CD). Additionally, OBS is significantly negatively correlated with intestinal obstruction in CD patients. Gender stratified analysis suggest a significant correlation between OBS and CD in female patients, particularly pronounced in those under 60 years old. Sensitivity analysis indicates a significant negative correlation between lifestyle-related OBS and diet-related OBS with the occurrence of CD in females, diet-related OBS is negatively correlated with CD in males.ConclusionOBS showed a significant negative correlation with IBD, especially in female CD patients. This study underscores the importance of antioxidant diet and lifestyle, which may provide a greater advantage for female CD patients.

BackgroundThe intricate interplay between dietary habits and the development of Parkinson’s Disease (PD) has long been a subject of scientific inquiry. Mendelian Randomization (MR) emerges as a potent tool, harnessing genetic variants to infer causality in observational data. While evidence links diet to Parkinson’s Disease (PD) etiology, a thorough MR exploration of dietary impacts on PD, particularly involving gut microbiota, is still emerging.MethodsThis research leverages the IEU Open GWAS project’s vast GWAS database1 to address the knowledge gap in understanding diet’s influence on PD, employing a diverse range of dietary variables. Our holistic dataset includes various foods like processed fava beans, bap, red wine, to cheese, reflecting a commitment to untangling dietary complexities in PD etiology. Advancing from initial dietary-PD associations, we innovatively explore the gut microbiota, focusing on Parabacteroides goldsteinii, in relation to bap intake and PD, employing MR. Utilizing weighted median, MR-Egger, and inverse variance weighting methods, we ensure rigorous causality assessments, meticulously mitigating pleiotropy and heterogeneity biases to uphold finding validity.ResultsOur findings indicate red wine (OR: 1.031; 95% CI 1.001–1.062; p = 0.044) and dried fruit consumption (OR: 2.019; 95% CI 1.052–3.875; p = 0.035) correlate with increased PD risk, whereas broad beans (OR: 0.967; 95% CI 0.939–0.996; p = 0.024) and bap intake (OR: 0.922; 95% CI 0.860–0.989; p = 0.023) show protective effects against PD. Employing MR, specifically the IVW method, revealed a significant inverse association between bap intake and gut microbiota, marked by an 8.010-fold decrease in Parabacteroides goldsteinii per standard deviation increase in bap intake (95% CI 1.005–63.818, p = 0.049). Furthermore, a connection between PD and Parabacteroides goldsteinii was observed (OR: 0.810; 95% CI 0.768–0.999; p = 0.049), suggesting a potential microbiota-mediated pathway in PD etiology.ConclusionOur study links dietary habits to PD risk, showing higher PD risk with red wine and dried fruit consumption, and a protective effect from broad beans and bap. Using MR, we found bap intake inversely correlates with Parabacteroides goldsteinii in the gut, suggesting bap influences microbiota. Further, higher Parabacteroides goldsteinii levels correlate with lower PD risk, highlighting a complex interplay of diet, gut microbiome, and neurological health. These insights shed light on potential dietary interventions for PD.

Interspecific hybridization in Vitis holds immense potential for combining valuable adaptive traits and breeding superior cultivar development. To evaluate the feasibility of hybrid breeding using the resilient wild species Vitis adenoclada and the commercially successful cultivar V. vinifera 'Shine Muscat', their signatures of hybridization, genetic divergence, and divergent selection were investigated. Analyses of 28 resequencing genomes revealed pronounced genetic differentiation between these two lineages and corroborated the hybridization event within a derived progeny. Notably, 'Shine Muscat' exhibited stronger genome-wide selection signals, reflecting its intensive breeding history. While divergent selection signatures associated with disease resistance were evident in both species, V. adenoclada displayed enrichment in pathways linked to abiotic stress resistance. Furthermore, while 'Shine Muscat' displayed potentially mitigated deleterious mutations compared to V. adenoclada, their hybrid offspring exhibited an accumulation of heterozygous deleterious alleles, emphasizing the crucial need for monitoring such mutations in future breeding endeavors. Collectively, the findings unveil the significant genetic divergence and contrasting adaptations between V. adenoclada and 'Shine Muscat', highlighting their immense potential for breeding next-generation cultivars with enhanced resilience and superior quality.

System operators rely on system flexibility to handle unexpected reliability and resilience events, ranging from excessive resource forecast errors to extreme events like heatwaves, earthquakes, and cyberattacks. This paper provides a production cost modeling (PCM) methodology to quantify contributions to system flexibility and economic benefits by controllable HVdc and multi-terminal HVdc (MTdc) transmission systems. First, the PCM model of a general MTdc grid is developed to be seamlessly added to existing scalable PCM model of an ac power system. Second, a method for modeling extreme operating conditions, including heatwave and wildfire, in PCM is presented. Finally, the planning 2030 Western Electricity Coordinating Council (WECC) system is used as an example to demonstrate the benefits of existing and future dc lines in point-to-point, radial, and meshed configurations. Under extreme system conditions including heatwave and wildfire, it is identified from the PCM simulation results that HVdc and MTdc transmission flexibility can provide substantial economic, reliability, and environmental benefits. These benefits include reductions up to 6.4% total generation cost, 8.6% unserved load, 50.3% renewable curtailment, 75% locational marginal price, and 4.0% CO2 emission amount.

This is an essay in the field of ceramics and composites. The iron tailings are replaced with river sand to prepare autoclaved concrete. The influence of cement dosage, fly ash dosage, iron tailing sand dosage and water reducing agent dosage on the fluidity and compressive strength of autoclaved concrete is analyzed. The concrete is autoclaved and cured. The hydration mechanism of concrete under different autoclave time is analyzed. The results show that considering economy and sample strength, as cement dosage of 551 kg/m3, iron tailing sand dosage of 20%, fly ash dosage of 225 kg/m3 and water reducing agent dosage of 0.20% is selected. Under the action of the above comprehensive indicators, the compressive strength of concrete is determined to be 61.13 MPa. The longer the autoclave time is, the more active the mineral components in the iron tailings will be stimulated, and the hydration reaction inside the concrete will be more thorough. The more crystals and gels are formed, which effectively improves the strength of concrete.

Abstract Reactive negative electrodes like lithium (Li) suffer serious chemical and electrochemical corrosion by electrolytes during battery storage and operation, resulting in rapidly deteriorated cyclability and short lifespans of batteries. Li corrosion supposedly relates to the features of solid-electrolyte-interphase (SEI). Herein, we quantitatively monitor the Li corrosion and SEI progression (e.g., dissolution, reformation) in typical electrolytes through devised electrochemical tools and cryo-electron microscopy. The continuous Li corrosion is validated to be positively correlated with SEI dissolution. More importantly, an anti-corrosion and interface-stabilizing artificial passivation layer comprising low-solubility polymer and metal fluoride is designed. Prolonged operations of Li symmetric cells and Li | |LiFePO4 cells with reduced Li corrosion by ~74% are achieved (0.66 versus 2.5 μAh h−1). The success can further be extended to ampere-hour-scale pouch cells. This work uncovers the SEI dissolution and its correlation with Li corrosion, enabling the durable operation of Li metal batteries by reducing the Li loss.

Different endogenous components (tocopherols, phytosterols, polyphenols and squalene) of vegetable oils were added to compounded vegetable oils to investigate the antioxidant effects. Using a Schaal oven-accelerated experiment, the effects of endogenous antioxidants on the oxidative stability of compounded oils were examined by changes of fatty acid composition, acid value, peroxide value, anisidine value, and total oxidative value. The results demonstrated that the individual tocopherols and polyphenols showed a substantial antioxidant impact, and the antioxidant effect increased with concentration. As the concentrations of tocopherols and squalene increased, the combined of tocopherols and squalene showed antagonistic antioxidant effects and then synergistic effects, and the presence of squalene significantly increased the antioxidant capacity of tocopherols when the tocopherol content was greater than 480 mg/kg (P

Abstract The gas pressure in front area of heading face is essential to dynamically evaluate coal and gas outburst during coal mining. In this work, a novel inversion model of gas pressure in front area of the heading face was established on premise of the hypothesis that a time-dependent zone of steady flow exists within newly exposed face. The key parameters in the inversion model were obtained based on the gas emission models and field data of gas emission rate in different times, which were used to calculate the volumes of gas emission from different sources. The results show that the percentage of gas emission from the heading face, coal wall and collapsed coal ranges from 7% to 47%, 47% to 82% and 2% to 11%, respectively. Based on the calculated volumes of gas emission and gas pressure inversion model, the gas pressure was obtained and transformed to the gas content. The absolute errors between the gas content tested and transformed in every hour is 0.4%–33%, which proved the rationality of gas pressure inversion model. Furthermore, the daily drifting footage, the radius of gas pressure boundary and the gas permeability coefficient of coal seam were confirmed to have a great effect on the result of gas pressure inversion. The inversion results verify that the speedy excavation can increase the risk of coal and gas outburst. This work produces a useful method for gas disaster prevention and control that converts the gas emission rate to an index of gas pressure within coal seam.

Abstract In recent years, lung adenocarcinoma (LUAD) has become a focus of attention due to its low response to treatment, poor prognosis, and lack of reliable indicators to predict the progression or therapeutic effect of LUAD. Different cell death patterns play a crucial role in tumor development and are promising for predicting LUAD prognosis. From the TCGA and GEO databases, we obtained bulk transcriptomes, single-cell transcriptomes, and clinical information. Genes in 15 types of cell death were analyzed for cell death index (CDI) signature establishment. The CDI signature using necroptosis + immunologic cell death-related genes was established in the TCGA cohort with the 1-, 2-, 3-, 4- and 5-year AUC values were 0.772, 0.736, 0.723, 0.795, and 0.743, respectively. The prognosis was significantly better in the low CDI group than in the high CDI group. We also investigated the relationship between the CDI signature and clinical variables, published prognosis biomarkers, immune cell infiltration, functional enrichment pathways, and immunity biomarkers. In vitro assay showed that HNRNPF and FGF2 promoted lung cancer cell proliferation and migration and were also involved in cell death. Therefore, as a robust prognosis biomarker, CDI signatures can screen for patients who might benefit from immunotherapy and improve diagnostic accuracy and LUAD patient outcomes.