Search

Abstract The regenerative treatment of infectious vertical bone defects remains difficult and challenging today. Current clinical treatments are limited in their ability to control bacteria and infection, which is unfavorable for new bone formation and calls for a new type of material with excellent osteogenic and antibacterial properties. Here a multifunctional scaffold is synthesized that mimics natural bone nanostructures by incorporating silver nanowires into a hierarchical, intrafibrillar mineralized collagen matrix (IMC/AgNWs), to achieve the therapeutic goals of inhibiting bacterial activity and promoting infectious alveolar bone augmentation in rats and beagle dogs. An appropriate concentration of 0.5 mg mL−1 AgNWs is selected to balance biocompatibility and antibacterial properties. The achieved IMC/AgNWs exhibit a broad spectrum of antimicrobial properties against Gram‐negative Porphyromonas gingivalis and Gram‐positive Streptococcus mutans. When the IMC/AgNWs are cocultured with periodontal ligament stem cells, it possesses excellent osteoinductive activities under both non‐inflammatory and inflammatory conditions. By constructing a rat mandibular infected periodontal defect model, the IMC/AgNWs achieve a near‐complete healing through the canonical BMP/Smad signaling. Moreover, the IMC/AgNWs enhance vertical bone height and osseointegration in peri‐implantitis in beagle dogs, indicating the clinical translational potential of IMC/AgNWs for infectious vertical bone augmentation.

Abstract Nano-engineering-based tissue regeneration and local therapeutic delivery strategies show significant potential to reduce the health and economic burden associated with craniofacial defects, including traumas and tumours. Critical to the success of such nano-engineered non-resorbable craniofacial implants include load-bearing functioning and survival in complex local trauma conditions. Further, race to invade between multiple cells and pathogens is an important criterion that dictates the fate of the implant. In this pioneering review, we compare the therapeutic efficacy of nano-engineered titanium-based craniofacial implants towards maximised local therapy addressing bone formation/resorption, soft-tissue integration, bacterial infection and cancers/tumours. We present the various strategies to engineer titanium-based craniofacial implants in the macro-, micro- and nano-scales, using topographical, chemical, electrochemical, biological and therapeutic modifications. A particular focus is electrochemically anodised titanium implants with controlled nanotopographies that enable tailored and enhanced bioactivity and local therapeutic release. Next, we review the clinical translation challenges associated with such implants. This review will inform the readers of the latest developments and challenges related to therapeutic nano-engineered craniofacial implants.

Stochastic heat conduction and thermal stress analysis of structures has received considerable attention in recent years. The propagation of uncertain thermal environments will lead to stochastic variations in temperature fields and thermal stresses. Therefore, it is reasonable to consider the variability of thermal environments while conducting thermal analysis. However, for ambient thermal excitations, only stationary random processes have been investigated thus far. In this study, the highly efficient explicit time-domain method (ETDM) is proposed for the analysis of non-stationary stochastic transient heat conduction and thermal stress problems. The explicit time-domain expressions of thermal responses are first constructed for a thermoelastic body. Then the statistical moments of thermal displacements and stresses can be directly obtained based on the explicit expressions of thermal responses. A numerical example involving non-stationary stochastic internal heat generation rate is investigated. The accuracy and efficiency of the proposed method are validated by comparison with the Monte-Carlo simulation. Keywords: Stochastic, Non-stationary, Heat conduction, Thermal stress, Explicit time-domain method

Purpose: To propose and evaluate a novel method for achieving a favorable bone-to-implant contact (BIC) area for zygomatic implants (ZIs). Materials and Methods: Patients who needed ZIs to restore a severely atrophied maxilla were recruited. In preoperative virtual planning, an algorithm was utilized to find the ZI trajectory that would achieve the largest BIC area with a predefined entry point on the alveolar ridge. The surgery was conducted according to the preoperative plan with the assistance of real-time navigation. Area BIC (A-BIC), linear BIC (L-BIC), distance from implant to infraorbital margin (DIO), distance from implant to infratemporal fossa (DIT), implant exit section, and deviation of the real-time navigated surgery were measured and compared between the preoperative plan and the placed ZIs. The patients were followed up for 6 months. Results: Overall, 11 patients with 21 ZIs were included. The A-BICs and L-BICs were significantly higher in the preoperative plan than in the placed implants (P < .05). Meanwhile, there were no significant differences in DIO or DIT. The planned-placed deviation was 2.31 ± 1.26 mm for the entry, 3.41 ± 1.77 mm for the exit, and 3.06 ± 1.68 degrees for the angle. All ZIs survived to the 6-month follow-up. Conclusion: This novel method can virtually calculate the trajectory of ZIs and transfer the preoperative plan to surgery to acquire a favorable BIC area. The actual positions of placed ZIs were slightly deviated from the ideal due to navigation errors. [ABSTRACT FROM AUTHOR]

Objective: This research aimed to study the inhibitory effect of xylitol on the growth and acid production of Actinomyces viscosus (A. viscosus)., Methods: We cultivated A. viscosus in anaerobic conditions with different concentrations (128, 64, 32, 16, 8, and 4 g x L(-1)) of xylitol brain heart infusion liquid medium and determined the minimum inhibitory concentration (MIC). Subsequently, we measured the pH value of the control group, as well as those of 1/2, 1/4, 1/8 MIC, and MIC concentration groups at 1.5, 3, 6, 12, 24, and 48 h. The Delta pH and OD550 at 2, 4, 6, 8, 10, and 12 h were calculated. We discovered that the minimum xylitol concentrations suppressed 50% and 90% A. viscosus biofilm formation (i.e., MBIC50 and MBIC90). SPSS 19.0 was used to analyze the collected data, and conclusions were drawn afterward., Results: Xylitol inhibited the growth ofA. viscosus at MIC of 64 g x L(-1). After 12 h, the differences of pH value among groups were all statistically significant (P < 0.05), and Delta pH increased when the MIC concentration decreased. Except for the 1/2 MIC and MIC groups, the differences of OD550 among groups had no statistical significance (P>0.05), and OD550 also increased when the MIC concentration decreased. These results imply that the ability ofA. viscosus to grow and produce acid in 1/2 MIC and MIC conditions will be reduced with the increase in xylitol concentration. The value of MIBC50 was 64 g x L(-1), whereas the value of MIBC90 was 128 g x L(-1). This finding indicates that the xylitol medium can restrict A. viscosus biofilm formation., Conclusion: Xylitolcan effectively inhibit the growth, adhesion, and acid production ofA. viscosus, protecting teeth from cariogenic bacteria and preventing caries to a certain extent.