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Abstract Infusion extravasation has an increased incidence in newborns, which can result in various adverse outcomes. This study aimed to investigate the effects of different types of temperament on infusion extravasation in newborns. A total of 209 newborns aged 4–7 days who were treated with infusion therapy were assessed for temperament type using the neonatal behavioral assessment scale score (NBAS). The 2009 Infusion Nurses Society clinical grading criteria for extravasation were used, and the clinical data of the newborns, such as gestational age and body weight, were collected. Out of 209 newborns assessed, 107 developed infusion extravasations, with an incidence rate of 51.2%. Newborns with intermediate temperament type were more prone to develop infusion extravasation. Newborns with low body weight, amniotic fluid aspiration syndrome, or meconium aspiration syndrome were prone to develop infusion extravasation. Body weight, temperament type of consolability, temperament type of peak of excitement, diseases, general temperament type, and NBAS total scores of the neonates were independent risk factors for infusion extravasation. Thus, different types of temperament can have an impact on neonatal extravasation.

CO2 geological storage is a guaranteed technology for achieving carbon neutrality. The offshore deposit basin is far away from the human activity area, and the implementation of a CO2 geological storage is more advantageous than the land sedimentary basin. To find out the CO2 storage potential in the Weixinan sag of the Beibu Gulf Basin, based on the 8 indicators such as caprock burial depth, the maximum thickness of single layer mudstone, mud-soil ratio, fault density and reservoir porosity, formation thickness, sand-soil ratio, permeability, etc., it has established a reservoir evaluation system for the secondary structure unit of the basin. Combined with the core physical property data of lays and 3D geological models, the reservoir and caprock of the Weixinan sag are optimized, and the effective storage potential is evaluated with the US-DOE method. The results show that within 800−3000 meters below the sea floor, in the Weixinan sag, there are two sets of reservoir-cap combinations suitable for CO2 storage. Combination 1: in Neogene, the neritic mudstone of the first member of the Jiaowei Formation is the cap rock, and the neritic sandstone of the second member of the Jiaowei Formation and the Xiayang Formation is the reservoir, and the effective storage capacity is 0.749 billion tons. Combination 2: in the Paleogene, the deep lake mudstone in the second member of the Weizhou Formation is the cap rock, and the sandstone in the third member of the Weizhou Formation is the reservoir, and the effective storage capacity is 0.374 billion tons. In the Weixinan sag, two sets of reservoir-cap assemblages suitable for CO2 storage in the saline aquifers are selected, with an effective storage capacity of 1.123 billion tons. According to the evaluation of the annual CO2 emission of 70 million tons in the west of Guangdong Province, it can meet the CO2 emission reduction needs of the region for more than 16 years. Offshore geological storage is a potential path for the green and sustainable development of coastal heavy chemical clustering areas and to achieve the goal of carbon neutrality.

Abstract Bleeding and thrombosis are known as common complications of polycythemia for a long time. However, the role of coagulation system in erythropoiesis is unclear. Here, we discover that an anticoagulant protein tissue factor pathway inhibitor (TFPI) plays an essential role in erythropoiesis via the control of heme biosynthesis in central macrophages. TFPI levels are elevated in erythroblasts of human erythroblastic islands with JAK2 V617F mutation and hypoxia condition. Erythroid lineage-specific knockout TFPI results in impaired erythropoiesis through decreasing ferrochelatase expression and heme biosynthesis in central macrophages. Mechanistically, the TFPI interacts with thrombomodulin to promote the downstream ERK1/2-GATA1 signaling pathway to induce heme biosynthesis in central macrophages. Furthermore, TFPI blockade impairs human erythropoiesis in vitro, and normalizes the erythroid compartment in mice with polycythemia. These results show that erythroblast-derived TFPI plays an important role in the regulation of erythropoiesis and reveal an interplay between erythroblasts and central macrophages.

In this study, high-throughput sequencing technology and statistical analysis were employed to explore the effects of adding green tea at proportions of 10%, 20%, and 30% on physicochemical characteristics, bacterial community diversity and succession of Daqu during fermentation compared with pure wheat Daqu (control). The results showed that the addition of green tea significantly increased the bacterial community diversity of Daqu and improved its saccharification capacity, moisture content and acidity. However, the increase in tea addition did not exhibit a proportional relationship with the community diversity. The unique evolutionary structure of the bacterial communities in different types of Daqu was found to be the major factor affecting the physicochemical characteristics of Daqu. During the fermentation process, all four Daqu exhibited differentiation in bacterial community succession starting from the thermophilic stage, thereby leading to the formation of their unique community structure at the end of the fermentation process. The phyla Proteobacteria, Firmicutes, Bacteroidota, and Actinobacteria showed varying influences on the community succession of the four Daqu samples, thus making each of them have different physicochemical characteristics. Specifically, Pseudomonas was an important bacterial genus contributing to the physicochemical characteristics of the control and 10% tea Daqu, while Weissella and Pediococcus, were important contributors to the physicochemical characteristics of the 20% tea Daqu. Bacillus was found to be a significant bacterial genus contributing to the physicochemical characteristics of the 30% tea Daqu. This study provides a theoretical basis for process optimization in the production of tea-added Daqu and for the development of multi-raw material fermentation starters. It also has implications for improving the preparation process of Daqu and the application of tea in Daqu making.

Point cloud generation and representation is important in industry areas. Generating and editing high quality 3D shapes is challenging work in deep learning. Inspired by StyleGAN, a style based generative adversarial networks is proposed to generate high quality 3D point cloud. An improved non-linear mapping network learn distribution of points and is used to generate well distributed point cloud point cloud. We also provide a coarse to fine representation for point cloud. According to the experimental results on the ShapeNet Part data set(including aircraft, chair single category and overall 16 categories), our method can generate more uniform point cloud than other GAN methods with less training epoches. The latent code for point cloud has better linear separation,and is more easy to edit.

With the deep involvement of Chinese oil enterprises in global oil production and the breaking of the integrated management mode of production, transportation and marketing for Chinese oil and gas companies, it's necessary to have a more comprehensive and systematic understanding of crude oil custody measurement. Herein, the five major standard systems of crude oil custody measurement worldwide were sorted out. Comprehensive analysis was conducted for the application of crude oil custody measurement standards in different regions of the world, their interrelationship, and the development of relevant Chinese standards in the oil and gas field. The overall management level concerning the selection of main parameters of custody measurement, the accuracy of relevant instrument and equipment, and their calibration and verification, were analyzed systematically by comparing the requirements of different standard systems in terms of the base condition, oil measurement system, oil test and the calibration of measuring equipment. In addition, four measurement design schemes were summarized by analyzing the design schemes for custody measurement of multiple in-service crude oil pipelines by classification. Further, the quality bank of crude oil was included in the design schemes to improve the measurement management level. Generally, the research results could provide a useful reference for the promotion of relevant oil and gas standards in the emerging region and the design of various custody measurement schemes.

1-Deoxy-d-xylulose-5-phosphate synthase and 1-deoxy-d-xylulose-5-phosphate reductoismerase are considered two key enzymes in the 2-C-methyl-d-erythritol-4-phosphate pathway of terpenoid biosynthesis and are related to the synthesis and accumulation of sesquiterpenoids. We cloned two DXS and DXR genes from Atractylodes lancea and analysed their expression in different tissues and in response to methyl jasmonate (MeJA). Subcellular localisation analysis revealed that the AlDXS and AlDXR1 proteins are located in the chloroplasts and cytoplasm, whereas AlDXR2 is only located in the chloroplasts. pET-AlDXS-28a and pGEX-AlDXR-4T-1 were expressed in Escherichia coli BL21(DE3) and BL21, respectively. Based on the abiotic stress analysis, the growth rate of the recombinant pGEX-AlDXR-4T-1 was higher than that of the control in HCl and NaOH. AlDXS exhibited the highest expression level in rhizomes of A. lancea from Hubei but was highest in leaves from Henan. In contrast, AlDXR showed maximum expression in the leaves of A. lancea from Hubei and Henan. Moreover, DXS and DXR gene expression, enzyme activities, and antioxidant enzyme activities oscillated in response to MeJA, with expression peaks appearing at different time points. Our findings indicated that the characterisation and function of AlDXS and AlDXR could be useful for further elucidating the functions of DXR and DXR genes in A. lancea. Key words: Atractylodes lancea, 1-deoxy-d-xylulose-5-phosphate synthase, 1-deoxy-d-xylulose-5-phosphate reductoismerase, subcellular localisation, gene expression, Introduction Terpenoids have a wide variety of pharmacological activities, including antimicrobial, antiallergenic, anticancer, anti-inflammatory, and immunomodulatory properties (Kuang et al. 2021). For example, the sesquiterpene lactone, artemisinin, from Artemisia annua [...]