15 results on '"Koltsova, Svetlana V."'
Search Results
2. Myogenic tone in mouse mesenteric arteries: evidence for P2Y receptor-mediated, Na+, K+, 2Cl− cotransport-dependent signaling
- Author
-
Koltsova, Svetlana V., Maximov, Georgy V., Kotelevtsev, Sergei V., Lavoie, Julie L., Tremblay, Johanne, Grygorczyk, Ryszard, Hamet, Pavel, and Orlov, Sergei N.
- Published
- 2009
- Full Text
- View/download PDF
3. Augmented gene expression triggered by Na+,K+-ATPase inhibition: Role of Ca2+i-mediated and −independent excitation-transcription coupling.
- Author
-
Smolyaninova, Larisa V., Koltsova, Svetlana V., Sidorenko, Svetlana V., and Orlov, Sergei N.
- Abstract
In rat vascular smooth muscle cells (RVSMC), 3-h Na + ,K + -ATPase inhibition by ouabain or in K + -free medium resulted in the inversion of the [Na + ] i /[K + ] i ratio and elevation up to 7-fold the content of Egr1, Atf3, Nr4a1 and Ptgs2 mRNAs. Ouabain increased the rate of 45Ca 2+ influx by 2-fold that was abolished by L-type voltage-gated Ca 2+ channel blocker nicardipine, but it was resistant to Na + /Ca 2+ exchanger inhibitor KB-R7943. To study the role of Ca 2+ -mediated signaling in the expression of Na + i /K + i -sensitive genes we used intracellular Ca 2+ chelator BAPTA and incubated RVSMC in Ca 2+ -free medium. The elevation of Nr4a1 and Ptgs2 expression triggered by ouabain was diminished in Ca 2+ -depeleted cells as well as in the presence of nicardipine and calmodulin antagonists A-7 and W-7. Ptgs2 expression was also suppressed by inhibitor of Ca 2+ /calmodulin-dependent protein kinase (CaMKII) KN-93 whereas increment of Nr4a1 content triggered by ouabain was attenuated by inhibitor of Ca 2+ /calmodulin-dependent protein phosphatase (calcineurin, CaN) cyclosporin A. Neither Ca 2+ depletion nor above listed compounds had any impact on the augmented expression of Egr1 and Atf3 in ouabain-treated RVSMC. Our results strongly suggest that dissipation of transmembrane gradient of monovalent cations increases Ptgs2 and Nr4a1 transcription via augment Ca 2+ influx through L-type Ca 2+ channels that, in turn, leads to CaMKII-mediated phosphorylation of CREB and calcineurin-mediated dephosphorylation of NFAT, respectively. Additional experiments should be performed to identify intermediates of Na + i ,K + i -mediated Ca 2+ -independent excitation-transcription coupling involved the regulation of Egr1 and Atf3 expression. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
4. Transcriptomic changes in Ca2+-depleted cells: Role of elevated intracellular [Na+]/[K+] ratio.
- Author
-
Koltsova, Svetlana V., Tremblay, Johanne, Hamet, Pavel, and Orlov, Sergei N.
- Abstract
Previously, we reported that Ca 2+ depletion increased permeability of the plasma membrane for Na + . This study examined the relative impact of [Na + ] i /[K + ] i -mediated signaling on transcriptomic changes in cultured vascular smooth muscle cells from rat aorta (VSMC) subjected to Ca 2+ -depletion by extra-(EGTA) and intracellular (BAPTA-AM) Ca 2+ chelators. Na + ,K + -ATPase inhibition in K + -free medium during 3 h led to elevation of [Na + ] i and attenuation of [K + ] i by ∼7- and 10-fold, whereas Ca 2+ -depletion resulted in alteration of these parameters by ∼3- and 2-fold, respectively. Augmented VSMC permeability for Na + and elevation of the [Na + ] i /[K + ] i ratio was triggered by addition to Ca 2+ -free medium 50 μM EGTA and was not affected by 10 μM BAPTA-AM. Na + ,K + -ATPase inhibition and Ca 2+ -depletion changed expression of 3677 and 4610 mRNA transcripts, respectively. We found highly significant ( p < 10 −12 ) positive ( R 2 > 0.51) correlation between levels of expression of 2071 transcripts whose expression was affected by both stimuli. Among genes whose expression in Ca 2+ -depleted cells was augmented by more than 7-fold we noted cyclic AMP-dependent transcription factor Atf3 , early growth response protein Egr1 and nuclear receptor subfamily 4, group A member Nr4a1. Dissipation of transmembrane gradients of monovalent cations in high-K + , low-Na + -medium abolished the increments of the [Na + ] i /[K + ] i ratio as well as the augmented expression of these genes triggered by incubation of VSMC in EGTA containing medium. Thus, our results demonstrate, for the first time, that robust transcriptomic changes triggered by Ca 2+ -depletion in the presence of extracellular Ca 2+ -chelators are at least partially mediated by elevation of the [Na + ] i /[K + ] i ratio and activation of Ca 2+ i -independent, [Na + ] i /[K + ] i -mediated mechanism of excitation–transcription coupling. These results shad a new light on analysis of data obtained in cells subjected to long-term exposure to Ca 2+ chelators. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
5. Transcriptomic Changes Triggered by Hypoxia: Evidence for HIF-1α -Independent, [Na+]i/[K+]i-Mediated, Excitation-Transcription Coupling.
- Author
-
Koltsova, Svetlana V., Shilov, Boris, Birulina, Julia G., Akimova, Olga A., Haloui, Mounsif, Kapilevich, Leonid V., Gusakova, Svetlana V., Tremblay, Johanne, Hamet, Pavel, and Orlov, Sergei N.
- Subjects
- *
HYPOXIA-inducible factor 1 , *GENETIC transcription , *CELLULAR signal transduction , *GENE expression , *REPERFUSION - Abstract
This study examines the relative impact of canonical hypoxia-inducible factor-1alpha- (HIF-1α and Na+i/K+i-mediated signaling on transcriptomic changes evoked by hypoxia and glucose deprivation. Incubation of RASMC in ischemic conditions resulted in ∼3-fold elevation of [Na+]i and 2-fold reduction of [K+]i. Using global gene expression profiling we found that Na+,K+-ATPase inhibition by ouabain or K+-free medium in rat aortic vascular smooth muscle cells (RASMC) led to the differential expression of dozens of genes whose altered expression was previously detected in cells subjected to hypoxia and ischemia/reperfusion. For further investigations, we selected Cyp1a1, Fos, Atf3, Klf10, Ptgs2, Nr4a1, Per2 and Hes1, i.e. genes possessing the highest increments of expression under sustained Na+,K+-ATPase inhibition and whose implication in the pathogenesis of hypoxia was proved in previous studies. In ouabain-treated RASMC, low-Na+, high-K+ medium abolished amplification of the [Na+]i/[K+]i ratio as well as the increased expression of all tested genes. In cells subjected to hypoxia and glucose deprivation, dissipation of the transmembrane gradient of Na+ and K+ completely eliminated increment of Fos, Atf3, Ptgs2 and Per2 mRNAs and sharply diminished augmentation expression of Klf10, Edn1, Nr4a1 and Hes1. In contrast to low-Na+, high-K+ medium, RASMC transfection with Hif-1a siRNA attenuated increments of Vegfa, Edn1, Klf10 and Nr4a1 mRNAs triggered by hypoxia but did not impact Fos, Atf3, Ptgs2 and Per2 expression. Thus, our investigation demonstrates, for the first time, that Na+i/K+i-mediated, Hif-1α- -independent excitation-transcription coupling contributes to transcriptomic changes evoked in RASMC by hypoxia and glucose deprivation. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
6. Increased Renal Epithelial Na Channel Expression and Activity Correlate With Elevation of Blood Pressure in Spontaneously Hypertensive Rats.
- Author
-
Haloui, Mounsif, Tremblay, Johanne, Seda, Ondrej, Koltsova, Svetlana V., Maksimov, Georgy V., Orlov, Sergei N., and Hamet, Pavel
- Abstract
Elevation of blood pressure with age is one of the hallmarks of hypertension in both males and females. This study examined transcriptomic profiles in the kidney of 12-, 40-, and 80-week-old spontaneously hypertensive rats and 4 recombinant inbred strains in search for functional genetic elements supporting temporal dynamics of blood pressure elevation. We found that both in males and females of spontaneously hypertensive rats and hypertensive recombinant inbred strains age-dependent blood pressure increment was accompanied by 50% heightened expression of epithelial sodium channel β- and γ-subunits. Epithelial sodium channel subunit expression correlated positively with blood pressure but correlated negatively with renin expression. Increased epithelial sodium channel activity was observed in cultured epithelial cells isolated from the kidney medulla of 80-week-old spontaneously hypertensive rats but not in age-matched normotensive Wistar Kyoto. This difference remained evident after 24-hour treatment with aldosterone.
22 Na uptake in the perfused kidney medulla was increased whereas the urinary Na/K ratio was decreased in old spontaneously hypertensive rats compared with normotensive controls. The difference was eliminated by the administration of epithelial sodium channel inhibitor benzamil. Observations in recombinant inbred strains representing various mixtures of parental hypertensive and normotensive genomes suggest that Scnn1g and Scnn1b genes themselves are not implicated in heightened expression and that the increased expression is neither secondary nor required for a partial elevation of blood pressure in contrast to spontaneously hypertensive rats. We suggest that spontaneously hypertensive rats display an intact negative feed-back between renin-angiotensin-system and epithelial Na channel activity whose upregulated expression is supported by a yet unknown mechanism. [ABSTRACT FROM AUTHOR]- Published
- 2013
- Full Text
- View/download PDF
7. NKCC1 and hypertension: Role in the regulation of vascular smooth muscle contractions and myogenic tone.
- Author
-
Orlov, Sergei N., Koltsova, Svetlana V., Tremblay, Johanne, Baskakov, Mikhail B., and Hamet, Pavel
- Abstract
High-ceiling diuretics (HCD), known potent inhibitors of housekeeping Na
+ ,K+ ,2Cl cotransporter (NKCC1) and renal-specific NKCC2, decrease [Cl− ]i , hyperpolarize vascular smooth muscle cells (VSMC), and suppress contractions evoked by modest depolarization, phenylephrine, angiotensin II, and UTP. These actions are absent in nkcc1/ knock-out mice, indicating that HCD interact with NKCC1 rather than with other potential targets. These findings also suggest that VSMC-specific inhibitors of NKCC1 may be considered potential pharmacological therapeutic tools in treatment of hypertension. It should be underlined that side by side with attenuation of peripheral resistance and systemic blood pressure, HCD blocked myogenic tone (MT) in renal afferent arterioles. Keeping this in mind, attenuation of MT might be a mechanism underlying the prevalence of end-stage renal disease documented in hypertensive African-Americans with decreased NKCC1 activity and in hypertensive patients subjected to chronic HCD treatment. The role of NKCC1-mediated MT in protection of the brain, heart, and other encapsulated organs deserves further investigation. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
8. Ubiquitous [Na+]i/[K+]i-Sensitive Transcriptome in Mammalian Cells: Evidence for Ca2+i-Independent Excitation-Transcription Coupling.
- Author
-
Koltsova, Svetlana V., Trushina, Yulia, Haloui, Mounsif, Akimova, Olga A., Tremblay, Johanne, Hamet, Pavel, and Orlov, Sergei N.
- Subjects
- *
ISCHEMIA , *GENETIC transcription , *BLOOD circulation disorders , *PHOSPHOPROTEIN phosphatases , *GENETIC regulation , *MUSCLE cells - Abstract
Stimulus-dependent elevation of intracellular Ca2+ ([Ca2+]i) affects the expression of numerous genes - a phenomenon known as excitation-transcription coupling. Recently, we found that increases in [Na+]i trigger c-Fos expression via a novel Ca2+i-independent pathway. In the present study, we identified ubiquitous and tissue-specific [Na+]i/[K+]i-sensitive transcriptomes by comparative analysis of differentially expressed genes in vascular smooth muscle cells from rat aorta (RVSMC), the human adenocarcinoma cell line HeLa, and human umbilical vein endothelial cells (HUVEC). To augment [Na+]i and reduce [K+]i, cells were treated for 3 hrs with the Na+,K+-ATPase inhibitor ouabain or placed for the same time in the K+- free medium. Employing Affymetrix-based technology, we detected changes in expression levels of 684, 737 and 1839 transcripts in HeLa, HUVEC and RVSMC, respectively, that were highly correlated between two treatments (p<0.0001; R2.0.62). Among these Na+ i/K+ i-sensitive genes, 80 transcripts were common for all three types of cells. To establish if changes in gene expression are dependent on increases in [Ca2+]i, we performed identical experiments in Ca2+-free media supplemented with extracellular and intracellular Ca2+ chelators. Surprisingly, this procedure elevated rather than decreased the number of ubiquitous and cell-type specific Na+i/K+i-sensitive genes. Among the ubiquitous Na+i/K+i-sensitive genes whose expression was regulated independently of the presence of Ca2+ chelators by more than 3-fold, we discovered several transcription factors (Fos, Jun, Hes1, Nfkbia), interleukin-6, protein phosphatase 1 regulatory subunit, dual specificity phosphatase (Dusp8), prostaglandin-endoperoxide synthase 2, cyclin L1, whereas expression of metallopeptidase Adamts1, adrenomedulin, Dups1, Dusp10 and Dusp16 was detected exclusively in Ca2+-depleted cells. Overall, our findings indicate that Ca2+i-independent mechanisms of excitation-transcription coupling are involved in transcriptomic alterations triggered by elevation of the [Na+]i/[K+]i ratio. There results likely have profound implications for normal and pathological regulation of mammalian cells, including sustained excitation of neuronal cells, intensive exercise and ischemia-triggered disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
9. Hyperosmotic and isosmotic shrinkage differentially affect protein phosphorylation and ion transport.
- Author
-
Koltsova, Svetlana V., Akimova, Olga A., Kotelevtsev, Sergei V., Grygorczyk, Ryszard, and Orlov, Sergei N.
- Subjects
- *
OSMOREGULATION , *PHOSPHORYLATION kinetics , *ION transport (Biology) , *PHOSPHOPROTEINS , *EPITHELIUM , *VASCULAR smooth muscle enzymes - Abstract
In the present work, we compared the outcome of hyperosmotic and isosmotic shrinkage on ion transport and protein phosphorylation in C11-MDCK cells resembling intercalated cells from collecting ducts and in vascular smooth muscle cells (VSMC) from the rat aorta. Hyperosmotic shrinkage was triggered by cell exposure to hypertonic medium, whereas isosmotic shrinkage was evoked by cell transfer from an hypoosmotic to an isosmotic environment. Despite a similar cell volume decrease of 40%-50%, the consequences of hyperosmotic and isosmotic shrinkage on cellular functions were sharply different. In C11-MDCK and VSMC, hyperosmotic shrinkage completely inhibited Na+,K+-ATPase and Na+,Pi cotransport. In contrast, in both types of cells isosmotic shrinkage slightly increased rather than suppressed Na+,K+-ATPase and did not change Na+,Pi cotransport. In C11-MDCK cells, phosphorylation of JNK1/2 and Erk1/2 mitogen-activated protein kinases was augmented in hyperosmotically shrunken cells by ~7- and 2-fold, respectively, but was not affected in cells subjected to isosmotic shrinkage. These results demonstrate that the data obtained in cells subjected to hyperosmotic shrinkage cannot be considered as sufficient proof implicating cell volume perturbations in the regulation of cellular functions under isosmotic conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
10. Activation of P2Y receptors causes strong and persistent shrinkage of C11-MDCK renal epithelial cells.
- Author
-
Koltsova, Svetlana V., Platonova, Alexandra, Maksimov, Georgy V., Mongin, Alexander A., Grygorczyk, Ryszard, and Orlov, Sergei N.
- Subjects
- *
CELL physiology , *CELL receptors , *EPITHELIAL cells , *PROTEIN kinase C , *CELL lines , *ION channels , *PURINERGIC receptors - Abstract
Purinergic receptors activate diverse signaling cascades and regulate the activity of cell volume-sensitive ion transporters. However, the effects of ATP and other agonists of P2 receptors on cell volume dynamics are only scarcely studied. In the present work, we used the recently developed dual-image surface reconstruction technique to explore the influence of purinergic agonists on cell volume in the C11-Madin-Darby canine kidney cell line resembling intercalated cells from kidney collecting ducts. Unexpectedly, we found that ATP and UTP triggered very robust (55-60%) cell shrinkage that lasted up to 2 h after agonist washout. Purinergic regulation of cell volume required increases in intracellular Ca2+ and could be partially mimicked by the Ca2+-ionophore ionomycin or activation of protein kinase C by 4β-phorbol 12-myristate 13-acetate. Cell shrinkage was accompanied by strong reductions in intracellular K+ and Cl- content measured using steady-state 86Rb+ and 36Cl- distribution. Both shrinkage and ion efflux in ATP-treated cells were prevented by the anion channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and by the BKCa channel inhibitors charybdotoxin, iberiotoxin, and paxilline. To evaluate the significance of cell-volume changes in purinergic signaling, we measured the impact of ATP on the expression of the immediate-early gene c-Fos. Thirty-minute treatment with ATP increased c-Fos immunoreactivity by approximately fivefold, an effect that was strongly inhibited by charybdotoxin and completely prevented by NPPB. Overall, our findings suggest that ATP-induced cell-volume changes are partially responsible for the physiological actions of purinergic agonists. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
11. Molecular origin of Na+/Li+ exchanger: Evidence against the involvement of major cloned erythrocyte transporters
- Author
-
Koltsova, Svetlana V., Trushina, Yulia A., Akimova, Olga A., Hamet, Pavel, and Orlov, Sergei N.
- Subjects
- *
MOLECULAR biology , *SODIUM channels , *LITHIUM ions , *ION exchange (Chemistry) , *ERYTHROCYTES , *BIOLOGICAL transport - Abstract
Abstract: Numerous studies have demonstrated heightened Na+/Li+ countertransport (NLCT) activity in erythrocytes of patients with essential hypertension or diabetic nephropathy. The same carrier also contributes to the therapeutic action of lithium salt, widely used in the treatment of psychiatric disorders. However, the molecular origin of NLCT remains unknown. This study examined the role of major ion transporters in NLCT by comparative analysis of its activity and that of ion transporters providing inwardly directed 86Rb, 22Na and 32P fluxes. NLCT was below the detection limit in rat erythrocytes and ∼50-fold higher in rabbits compared to humans. Unlike NLCT, the activities of Na+,K+-ATPase, Na+,K+,2Cl− cotransporter and anion exchanger were somewhat similar in the erythrocytes of these species, whereas Na+,Pi cotransport was in 1:2:6 proportion in rats, humans and rabbits, respectively. Loading of erythrocytes with Li+ for NLCT measurement did not affect the activity of Na+,Pi cotransporter. Keeping in mind that NLCT is much higher in rabbits vs humans and rats, we compared the set of membrane proteins in these species using 2-dimensional gel electrophoresis. This approach revealed 174 common spots, whereas 132 proteins were detected only in human and rabbit erythrocyte membranes. Among these proteins, we found 17 spots whose expression was higher by more than 5-fold in rabbit compared to human erythrocytes. Thus, our results argue against the involvement of major ion transporters in NLCT. They also show that comparative proteomics is a potent tool to identify the molecular origin of this carrier. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
12. Excitation–contraction coupling in resistance mesenteric arteries: Evidence for NKCC1-mediated pathway
- Author
-
Koltsova, Svetlana V., Kotelevtsev, Sergei V., Tremblay, Johanne, Hamet, Pavel, and Orlov, Sergei N.
- Subjects
- *
MESENTERIC artery , *BUMETANIDE , *MUSCLE contraction , *DIURETICS , *LABORATORY mice , *ENZYME inhibitors , *NITRIC oxide - Abstract
Abstract: Bumetanide and other high-ceiling diuretics (HCD) attenuate myogenic tone and contractions of vascular smooth muscle cells (VSMC) triggered by diverse stimuli. HCD outcome may be mediated by their interaction with NKCC1, the only isoform of Na+, K+, 2Cl− cotransporter expressed in VSMC as well as with targets distinct from this carrier. To examine these hypotheses, we compared the effect of bumetanide on contractions of mesenteric arteries from wild-type and NKCC1 knockout mice. In mesenteric arteries from wild-type controls, 100μM bumetanide evoked a decrease of up to 4-fold in myogenic tone and contractions triggered by modest [K+]o-induced depolarization, phenylephrine and UTP. These actions of bumetanide were preserved after inhibition of nitric oxide synthase with NG-nitro-l-arginine methyl ester, but were absent in mesenteric arteries from NKCC1-/- mice. The data show that bumetanide inhibits VSMC contractile responses via its interaction with NKCC1 and independently of nitric oxide production by endothelial cells. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
13. HCO3-Dependent Impact of Na+,K+,2Cl- Cotransport in Vascular Smooth Muscle Excitation-Contraction Coupling.
- Author
-
Koltsova, Svetlana V., Luneva, Oksana G., Lavoie, Julie L., Tremblay, Johanne, Maksimov, Georgy V., Hamet, Pavel, and Orlov, Sergei N.
- Subjects
- *
MESENTERIC artery , *CALCIUM channels , *BUMETANIDE , *VASCULAR smooth muscle , *SMOOTH muscle - Abstract
In smooth muscles, inhibition of Na+,K+,2Cl- cotransport (NKCC) by bumetanide decreased intracellular Cl- content ([Cl-]i) and suppressed the contractions triggered by diverse stimuli. This study examines whether or not bicarbonate, a regulator of several Cl- transporters, affects the impact of NKCC in excitation-contraction coupling. Addition of 25 mM NaHCO3 attenuated the inhibitory action of bumetanide on mesenteric artery contractions evoked by 30 mM KCl and phenylephrine (PE) by 5 and 3-fold, respectively. In cultured vascular smooth muscle cells, NaHCO3 almost completely abolished inhibitory actions of bumetanide on transient depolarization and [Ca2+]i elevation triggered by PE. In bicarbonate-free medium, bumetanide decreased [Cl-]i by ∼15%; this effect was almost totally abrogated by NaHCO3. The addition of NaHCO3 resulted in 2-fold inhibition of NKCC activity and 3-fold attenuation of [Cl-]i. These data strongly suggest that extracellular HCO3- diminishes the NKCC-sensitive component of excitation-contraction coupling via suppression of this carrier. Copyright © 2009 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
14. Vascular Smooth Muscle Contraction Evoked by Cell Volume Modulation: Role of the Cytoskeleton Network.
- Author
-
Koltsova, Svetlana V., Gusakova, Svetlana V., Anfinogenova, Yana J., Baskakov, Mikhail B., and Orlov, Sergei N.
- Subjects
- *
CYTOPLASMIC filaments , *MICROTUBULES , *SMOOTH muscle , *ACTOMYOSIN , *ANTINEOPLASTIC agents , *CETUXIMAB , *FLUORESCENCE microscopy , *MUSCLE cells - Abstract
Previously, we reported that hyposmotic swelling evoked transient vascular smooth muscle cell (SMC) contraction that was completely abolished by L-type Ca2+ channel blockers. In contrast, sustained contraction revealed in hyper- and isoosmotically-shrunken SMCs was insensitive to L-type channel blockers and was diminished in Ca2+-free medium by only 30-50%. Several research groups reported cell volume-dependent cytoskeleton network rearrangements. This study examines the role of cytoskeleton proteins in cell volume-dependent contraction of endothelium-denuded vascular smooth muscle rings (VSMR) from the rat thoracic aorta. Hyperosmotic shrinkage and hyposmotic swelling were triggered by modulation of medium osmolality; isosmotic shrinkage was induced by VSMR transfer from hypo- to isosmotic medium. The relative content of globular (G) and fibrillar (F) actin was estimated by fluorescence microscopy. Hyperosmotic shrinkage and hyposmotic swelling led to elevation of the F-actin/G-actin ratio by 2.5- and 1.8-fold respectively. Contraction of shrunken and swollen VSMR was insensitive to modulators of microtubules such as vinblastine, colchicine and docetaxel. Microfilament disassembly by cytochalasin B resulted in dramatic attenuation of the maximal amplitude of contraction of hyperosmotically-shrunken and hyposmotically-swollen VSMR, and almost completely abolished the contraction triggered by isosmotic shrinkage. These data suggest that both L-type Ca2+ channel-mediated contraction of swollen vascular SMC and Ca2+o-insensitive contractions of shrunken cells are triggered by reorganization of the microfilament network caused by elevation of the F-actin/G-actin ratio. Copyright © 2008 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
15. Time- and dose dependent actions of cardiotonic steroids on transcriptome and intracellular content of Na+ and K+: a comparative analysis.
- Author
-
Klimanova, Elizaveta A., Tverskoi, Artem M., Koltsova, Svetlana V., Sidorenko, Svetlana V., Lopina, Olga D., Tremblay, Johanne, Hamet, Pavel, Kapilevich, Leonid V., and Orlov, Sergei N.
- Abstract
Recent studies demonstrated that in addition to Na
+ ,K+ -ATPase inhibition cardiotonic steroids (CTSs) affect diverse intracellular signaling pathways. This study examines the relative impact of [Na+ ]i /[K+ ]i -mediated and -independent signaling in transcriptomic changes triggered by the endogenous CTSs ouabain and marinobufagenin (MBG) in human umbilical vein endothelial cells (HUVEC). We noted that prolongation of incubation increased the apparent affinity for ouabain estimated by the loss of [K+ ]i and gain of [Na+ ]i . Six hour exposure of HUVEC to 100 and 3,000 nM ouabain resulted in elevation of the [Na+ ]i /[K+ ]i ratio by ~15 and 80-fold and differential expression of 258 and 2185 transcripts, respectively. Neither [Na+ ]i /[K+ ]i ratio nor transcriptome were affected by 6-h incubation with 30 nM ouabain. The 96-h incubation with 3 nM ouabain or 30 nM MBG elevated the [Na+ ]i /[K+ ]i ratio by ~14 and 3-fold and led to differential expression of 880 and 484 transcripts, respectively. These parameters were not changed after 96-h incubation with 1 nM ouabain or 10 nM MBG. Thus, our results demonstrate that elevation of the [Na+ ]i /[K+ ]i ratio is an obligatory step for transcriptomic changes evoked by CTS in HUVEC. The molecular origin of upstream [Na+ ]i /[K+ ]i sensors involved in transcription regulation should be identified in forthcoming studies. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.