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ObjectiveTo improve the response capabilities to disasters and prevent major epidemics， it is of practical use to study the capability evaluation system of the national emergency medical rescue team that combines theoretical training and practical exercises， to enhance the overall quality of the teams.MethodsFirst， a capability assessment system for the national emergency medical rescue team was constructed based on the INSARAG External Classification （IEC） standards of the national emergency medical rescue team. Then， based on the outcome based education （OBE） concept， we conducted innovative research on the curriculum design and exercise programs for team building and member training. Finally， an empirical analysis was conducted on the effectiveness of the evaluation system and training exercises based on the statistical analysis of the comprehensive quality evaluation of the Shanghai national emergency medical rescue team from 2020 to 2023， as well as the empirical analysis of the rescue exercise on the Cruise of spectrum.ResultsBased on the linear regression analysis of each core competency indicators， the five core competencies in the evaluation system， including rescue skills， medical and health knowledge， disaster coping ability， team cooperation ability， and mental resilience training， were positively correlated with the cumulative number of trainings （r=0.71， r=0.76， r=0.81， r=0.84， r=0.96，all P

The efficiency of the pump device is an important parameter to judge the overall dynamic performance of the pumping station. The commonly used method at home and abroad is to carry out model tests of the pump device. The performance parameters of the prototype pump and pump device are obtained by the similarity conversion formula. However, at present, there are not many device model tests for large vertical submersible pumps. Taking a large vertical submersible mixed-flow pumping station in China as an example, research predicted the performance of the pump device through a model test and a submersible pump prototype test. The results show that the model test of the large vertical submersible mixed-flow pump device has a maximum efficiency of approximately 77.8%, and the prototype test conversion device has a maximum efficiency of approximately 80.33%. The device model test and the pump factory prototype test results are compared. It is found that the performance parameters of the pump measured by the prototype test are in good agreement with the device model test under the design conditions, and there is a certain error when the deviation from the design conditions is significant. The device model test and the factory test of the pump are indispensable in the large-scale road of submersible pumps, and a large number of tests are needed to sum up the experience.

Objective To investigate the effect and mechanism of human umbilical cord mesenchymal stem cell-derived exosomes (HucMSCs-exos) on renal ischemia/reperfusion (I/R) injury in rats based on ferroptosis-related SLC7A11/GPX4 pathway. Methods HucMSCs were cultured and exosomes were obtained through differential centrifugation. The morphology of exosomes was observed by transmission electron microscopy, and the particle size was detected by nanoparticle tracking technology. Western blotting was used to determine the expression levels of surface marker proteins. The rat model of renal I/R injury was established by clamping both kidneys for 45 min, followed by reperfusion for 24 h. Thirty male SD rats (7 to 8 weeks old, 200 to 230 g) were randomly divided into 5 groups (n=6): Sham group, Sham+erastin group (SLC7A11 inhibitor), I/R group, Exo group, and Exo+erastin group. The Sham+erastin and Exo+erastin groups were intraperitoneally injected with erastin 10 mg/kg 30 min before modeling. The Exo group and Exo+erastin group were injected with HucMSCs-exos 20 μg via caudal vein 15 min before renal pedicle clamping. The renal samples were harvested 24 h after recovery and reperfusion. The levels of serum creatinine (Cr) and blood urea nitrogen (BUN) were detected. HE staining was employed to observe the pathological changes in the kidney tissue, and Paller score were calculated. The levels of ferroptosis-related markers, divalent ferrous ion (Fe2+), malondialdehyde (MDA), reactive oxygen species (ROS) and glutathione (GSH) in the renal tissues were detected. Western blotting was used to assess the expression levels of the ferroptosis-related protein SLC7A11 and glutathione peroxidase 4 (GPX4) in the kidney tissue. Results Compared with the Sham group, increased serum BUN and Cr levels (P < 0.05), obvious pathological injury in kidney tissue, elevated Paller score, Fe2+ and MDA contents (P < 0.05), increased ROS level (P < 0.05), and decreased GSH content and SLC7A11 and GPX4 protein levels (P < 0.05) were observed in the I/R group. Exo treatment reversed above changes when compared with the I/R group (all P < 0.05). The protective effects of HucMSCs-exo on ferroptosis and renal I/R injury were significantly attenuated by administration of erastin, a SLC7A11 inhibitor. Conclusions HucMSCs-exos can significantly attenuate renal I/R injury and play a protective role, and its mechanism may be related to the inhibition of ferroptosis by up-regulating the SLC7A11/GPX4 pathway.