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Abstract Background Hypoxia and ferroptosis are crucial in the occurrence and development of hepatocellular carcinoma (HCC), and they both affect the immune status of the tumor microenvironment. Previous studies have also shown a link between hypoxia and ferroptosis. Patients and methods In all, 814 HCC cases from The Cancer Genome Atlas and Gene Expression Omnibus databases were used as the discovery cohort, and 230 HCC cases from the International Cancer Genome Consortium database were used as the validation cohort. Hypoxia subtypes and ferroptosis subtypes were identified by consensus cluster analysis according to 174 hypoxia‐related genes and 193 ferroptosis‐related genes. The prognostic signature was constructed using the Cox and LASSO regression analyses, and two risk groups were identified. A comprehensive analysis of the clinical and immune characteristics between the two risk groups was further performed. Results Two hypoxia subtypes and two ferroptosis subtypes were distinguished and verified; subsequently, a five‐gene prognostic signature was constructed and the risk score could be acquired by the following formula: risk score = 0.0604*Expression (CA9)−0.0714*Expression (ANXA10) + 0.1501*Expression (CDC20)−0.0853*Expression (CYP7A1) + 0.0530*Expression (SPP1). Compared with the low‐risk group, the high‐risk group had a worse prognosis. The high‐risk group also showed a higher level of immune infiltration than the low‐risk group, and immune checkpoints were generally upregulated in the high‐risk group. The antigen presentation ability of the low‐risk group was poor, which may be related to the immune escape mechanism. Drug sensitivity analysis indicated that the high‐ and low‐risk groups were sensitive to tyrosine kinase inhibitors and chemotherapeutic drugs, respectively. Conclusion The hypoxia‐, ferroptosis‐, and immune‐associated prognostic signature we constructed could stratify patients with HCC and guide precise treatment.

Vanadium oxide cathode materials with stable crystal structure and fast Zn2+ storage capabilities are extremely important to achieving outstanding electrochemical performance in aqueous zinc‐ion batteries. In this work, a one‐step hydrothermal method was used to manipulate the bimetallic ion intercalation into the interlayer of vanadium oxide. The pre‐intercalated Cu ions act as pillars to pin the vanadium oxide (V‐O) layers, establishing stabilized two‐dimensional channels for fast Zn2+ diffusion. The occupation of Mn ions between V‐O interlayer further expands the layer spacing and increases the concentration of oxygen defects (Od), which boosts the Zn2+ diffusion kinetics. As a result, as‐prepared Cu0.17Mn0.03V2O5−□·2.16H2O cathode shows outstanding Zn‐storage capabilities under room‐ and low‐temperature environments (e.g., 440.3 mAh g−1 at room temperature and 294.3 mAh g−1 at −60°C). Importantly, it shows a long cycling life and high capacity retention of 93.4% over 2500 cycles at 2 A g−1 at −60°C. Furthermore, the reversible intercalation chemistry mechanisms during discharging/charging processes were revealed via operando X‐ray powder diffraction and ex situ Raman characterizations. The strategy of a couple of 3d transition metal doping provides a solution for the development of superior room‐/low‐temperature vanadium‐based cathode materials. [ABSTRACT FROM AUTHOR]

The complex in-cylinder gas state and flow significantly affect fuel-air mixture, combustion efficiency and emissions. However, the scavenging CFD model of large bore marine engines in digital twin system requires substantial computational resources. Therefore, a fast-run phenomenological model of the scavenging process is built in this study. The model simulates the thermal states and flow dynamics of inlet and exhaust gas based on the energy and momentum conservation principles, considering the ideal in-cylinder swirl velocity profile with effects of air mass loss, wall friction, and swirl shear. The model's accuracy is confirmed by comparing it with CFD simulations on different engines, showing an average relative error less than 3.5%. It also analyzes the impacts of intake pressure. The model provides accurate boundary conditions for subsequent fuel spray, combustion, and emission simulations and can be combined with these models in the future, thereby applied to engine design, diagnostics, and control. [ABSTRACT FROM AUTHOR]

Heterocyclic compounds were widely used in many domains; pyrrolidone is a derivative of heterocycles that can be used to synthesize anticancer drugs. A new fluorine-containing rhopaladins’ analog(E)-2-(4-bromobenzoyl)-N-(tert-butyl)-4-(4-fluoro benzylidene)-5-oxo-1-propylpyrrolidine-2-carboxamide (RPDPD for short) of 2-aroyl-4-arylidene-5-oxopyrrolidine derivative was synthesized by the one-pot synthesis method and evaluated for its anti-tumor activity in vitro via CCK8 assay and annexin V/propidium iodide (PI) staining of HeLa cells. The results exhibited that compound RPDPD has inhibited the proliferation of HeLa in a dose-dependent manner with an IC50 of 24.23 μmol/L (p < 0.05) and has low hepatotoxicity with an IC50 of 235.6 μmol/L (p < 0.05) to normal hepatocyte LO2 cells. The apoptotic assay demonstrated that compound RPDPD has induced apoptosis in HeLa cells (from 14.26 to 23.4%, p < 0.05). qRT-PCR results showed that the compound RPDPD could inhibit the expression of oncogene E6/E7 mRNA (p < 0.05) of human papillomavirus (HPV). The results of Western blot showed that the compound RPDPD promoted the expression of TIMP3 protein and inhibited the expression of MMP3 (p < 0.05). In conclusion, the compound RPDPD can inhibit the proliferation of cervical cancer cells and induce the apoptosis of cervical cancer cells, and its mechanism may be related to the inhibition of E6 mRNA and E7 mRNA expressions, and the anticancer effect of the compound RPDPD on cervical cancer is closely related to the TIMP3/MMP3 signaling axis.

In large-bore two-stroke diesel/nature gas dual-fuel marine engines, a certain quantity of diesel is injected into the cylinder to satisfy the full-power output engine rated power of the gas mixture. However, the ignition and flame propagation process based on the injection strategy of diesel direct injection combined with diesel jet flame on the ignition and combustion of natural gas is unclear, which directly affects the power and the thermal efficiency of engine and emissions. Therefore, this work numerically investigates the flame propagation characteristic under the strategy of the main and pilot diesel modes. The influence of the injection timing and proportion of diesel on combustion and emission performance are further analyzed. The results show that the influence of the injection timing of main diesel (MDIT) on the combustion process and emission performance is more obvious than that of the injection timing of pilot diesel (PDIT). The results indicate that the MDIT increased from −2°CA to −8°CA, the power increased by 316 kW, and the thermal efficiency improved by 1.5%. However, the CO2 emissions increased by 10.5 g/kWh, and the NOx emissions increased by 0.7 g/kWh. Additionally, an early PDIT is not conducive to the rapid organization of combustion, resulting in decreased engine power and thermal efficiency. Furthermore, it was found that the power improved by 50 kW and the thermal efficiency improved by 0.6%, with a decrease in the main diesel ratio (MDR) from 100% to 90%. Meanwhile, the CO2 emissions decreased by 4 g/kWh, although there was no obvious change in NOx emissions with the advance of MDR. [ABSTRACT FROM AUTHOR]