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Presents information on a study which examined B cell reconstitution in a severe combined immunodefficiency disease (SCID) patient who was treated with in utero haploidentical bone marrow transplant. Information on the treatment of SCID; Materials and methodology of the study; Results and discussion.

Background Partial and extensive hydrolysed protein formulae have been developed to lower or eliminate the antigenicity of' milk protein\. Although normally well tolerated. extensive hydrolysates have been reported to induce serious allergic reactions in very sensitive children. Moreover, clinical practice has often raised concern about the role of partial hydrolysates in Cow's milk allergy prevention .Objective: Strating from these considerations. we used anti-casein monoclonal anti- bodies to evaluate the presence of residual antigenic activity in both partially and extensively protein hydrolysates. Methods: Electrophoretic analyses associated with immunoblotting technique we it performed using nine protein-enriched commercial formulae. ResuIts: The presence of different amounts of residual intact cow's milk proteins and or polypeptidic material with conserved antigenic activity (according to the extensive or partial hydrolysis) was verified in most milk-based samples considered. Conclusion: The use of monoclonal antibodies and immunoblotting could be useful For the quality control of commercial `hypoallergemie' formulate. [ABSTRACT FROM AUTHOR]

A simple technique has been worked out for the simultaneous assessment of cell proliferation, generation of plasma cells, plaque-forming cells and immunoglobulin production from single cultures of 1 × 106 human peripheral blood lymphocytes stimulated with pokeweed mitogen (PWM). Kinetic studies showed that number of cells, thymidine incorporation rate, number of plaque-forming cells against fluorescein-isothiocyanate-haptenated sheep erythrocytes and concentration of IgM and IgG in the supernatant peaked on the average between day 5 and 7 of culture. This technique is particularly suitable for the analysis of hypogammaglobulinaemias in infancy. [ABSTRACT FROM AUTHOR]