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Two microbial strains Bacillus subtilis HDJ1 and Bacillus velezensis HDJ2 were screened from soybean paste samples by corn gluten meal medium for high protease activity. Single factor fermentation experiment showed that the optimum conditions of liquid fermentation of corn gluten meal for strain HDJ1 were: fermentation temperature of 40 ℃, fermentation pH 8, inoculum of 6%, substrate concentration of 8%, rotating speed of 200 r/min, liquid-adding volume of 40 mL, fermentation time of 72 h; The optimum conditions for liquid fermentation of corn gluten meal for strain HDJ2 were: fermentation temperature of 40 ℃, fermentation pH 6, inoculum of 9%, substrate concentration of 8%, rotating speed of 150 r/min, liquid-adding volume of 120 mL, fermentation time of 72 h; The optimum conditions for the fermentation of corn gluten meal for equal combination of HDJ1 and HDJ2 were: fermentation temperature of 30 ℃, fermentation pH 8, inoculum amount of 6%, substrate concentration of 8%, rotating speed of 250 r/min, liquid-adding volume of 40 mL, fermentation time of 96 h. The results of Orthogonal test showed that the content of soluble protein of HDJ1, HDJ2, HDJ1 and HDJ2 was the highest when the fermentation temperature was 30 ℃, the fermentation pH was 8, the inoculum amount was 3%, the substrate concentration was 8%, therotation speed was 200 r/min, and the fermentation time was 96 h. The protease activity, soluble protein content and DPPH scavenging rate of the sample significantly increased after freeze-drying of the microbial fermentation, and the fermentation effect of the two combined stains was better than that of single strain.

Intestinal colonization with carbapenem-resistant Enterobacterales (CRE) has been shown as a significant risk factor for subsequent CRE infections, especially in intensive care units (ICUs). The aim of this study was to determine the prevalence of intestinal CRE colonization among ICU patients in a Chinese tertiary hospital. Fecal sample screenings for CRE were performed on ICU patients weekly. Antibiotic-susceptibility profile of CRE strains was determined using the Vitek-2 analysis system and broth microdilution method. The carbapenemases of all isolates were determined by phenotypes and genotypes. Clonal relatedness was analyzed by pulsed-field gel electrophoresis (PFGE). Whole-genome sequencing was used to identify the multilocus sequence type (ST), plasmid replicons, and insertion sequences (ISs) of isolates. The overall colonization rate of CRE was 40.4% (82/203). A total of 84 CRE strains were detected, mostly with Klebsiella pneumoniae (92.9%). Antibiotic susceptibility testing profile revealed that 84 CRE strains were resistant to most antibiotics except for tigecycline and colistin. The carbapenemase-encoding genes including blaKPC-2, blaNDM-1, and blaIMP-4 were detected, and blaKPC-2 was the predominant genotype (90.8%). A total of 9 STs were identified among 84 CRE strains, and ST11 was the most common type (83.3%). A variety of mobile genetic elements, including plasmids and ISs, were detected via online tool prediction. PFGE analysis of the 78 K. pneumoniae strains showed 8 different pulsotypes, and pulsotype A was highly prevalent. This study found that the prevalence of CRE colonization was alarmingly high in the ICU, and that effective infection control measures are urgently needed to prevent the dissemination of CRE. [ABSTRACT FROM AUTHOR]

Two new furanoeremophilane sesquiterpenoids, namely, 6,9-dioxo-1α,4α-dihydroxy-furanoeremophilane (1) and 4α,5α-epoxy-6,9-dioxo-1α-hydroxyl-furanoeremophilane (2), and 10 known compounds were isolated from the whole plant of Chloranthus multistachys, and compound 3 was converted to derivative 3a. Their structures were determined based on extensive spectroscopic analysis. All compounds were evaluated by using five cancer cell lines: PC3, LNcap, A549, K562, and HEL. The derivative 3a exhibited excellent cytotoxic activities, with the IC50 against HEL cells being the lowest at 1.322 ± 0.08 μM, which was comparable to that of the positive control (doxorubicin). Mechanism studies showed that the anticancer activity of 3a may be associated with cell cycle regulation. [ABSTRACT FROM AUTHOR]

Aim: This study aimed to examine the differences in health status between patients with confirmed COVID‐19 and those suspected (other diagnosis) and to identify nursing diagnoses using a structured checklist from a hospital in China. Design: Cross‐sectional study design was used. Methods: One hundred sixty COVID‐19 confirmed, and suspected patients were conveniently selected. A structured survey and checklist were utilized. Independent t test and chi‐square test were employed to compare the mean between patients with confirmed coronavirus infection and others. A two‐sided p‐value of.05 or less is considered statistically significant. Results: The study yielded a response rate of 93.6%. The result indicated that patients with confirmed coronavirus infection have a higher proportion of perceived General Health Status than inpatients with suspected (other) diagnoses. The finding also indicated that ineffective airway clearance, hyperthermia, imbalanced nutrition less than body requirement and sleep pattern disturbance were the main nursing diagnoses identified. [ABSTRACT FROM AUTHOR]

The mass-selected infrared photodissociation (IRPD) spectroscopy was utilized to investigate the interactions of cationic cobalt with carbon dioxide molecules. Quantum chemical calculations were performed on the [Co(CO2)n]+ clusters to identify the structures of the low-lying isomers and to assign the observed spectral features. All the [Co(CO2)n]+ (n=2−6) clusters studied here show resonances near the CO2 asymmetric stretch of free CO2 molecule. Experimental and calculated results indicate that the CO2 molecules are weakly bound to the Co+ cations in an end-on configuration via a charge-quadrupole electrostatic interaction. The present IRPD spectra of [Co(CO2)n]+ clusters have been compared to those of Ar-tagged species ([Co(CO2)n]+-Ar), which would provide insights into the tagging effect of rare gas on the weakly-bounded clusters. [ABSTRACT FROM AUTHOR]