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This paper analysed Sickle cell anaemia literature on Nigeria listed in PubMed to understand the research performance of medical researchers on sickle cell anaemia literature on Nigeria. Data covering the period of 2006-2016 were collected from the PubMed, a citation database developed by the National Centre for Biotechnology Information (NCBI).The search strategy used was ((sickle cell anaemia) OR (sickle cell anemia)) AND Nigeria AND (("2006/01/01"[PDat] : "2016/12/31"[PDat])), bearing in mind variance in spelling (anaemia and anemia), which retrieved 326 sickle cell literature on Nigeria between 2006 and 2016. Article productivity increased between 2006 and 2010. However, there was a drastic drop in article publication between 2010 and 2011 as well as between 2013 and 2015. The degree of collaboration ranged between 0.85 and 1. University of Nigeria Teaching Hospital, Enugu, Nigeria, had the highest number of contributing authors. Authors published more in journals located in Nigeria, with Nigeria Journal of Clinical Practice being the most prolific publication outlet for authors publishing sickle cell anaemia literature on Nigeria. [ABSTRACT FROM AUTHOR]

Background: Calotropis procera leaf is one of the plants commonly utilized in phytomedicine in Nigeria. The present investigation explored the use of the extracts on cell viability and apoptosis respectively. In this study, the expression of the Cluster of differentiation 146 (CD146) in the blood of lung cancer patients on regulatory T cells (Tregs) was determined. The antioxidant and anti-proliferative effects of methanol extracts of Caloropis procera leaf on lung cancer cell H1299 were investigated. Methods: From the flow cytometry, the expression of the CD146+ in the T cells were evaluated using the healthy patient, adenocarcinoma, squamous, and small cell lung cancer respectively. The apoptosis of granulocytes, monocytes, lymphocytes, CD4+, and Treg were determined by 7-amino-actinomycin D/Annexin V-Allophycocyanin (APC) staining during the resting stage and after 24 h respectively. Immunofluorescence was conducted. Cell viability assay, hydroxyl (OH), hydrogen peroxide (H2O2) and nitric oxide (NO) scavenging radicals were conducted. Reducing power and flavonoid content of Calotropis procera were investigated. The effect of the Calotropis procera at different concentrations at 24hrs was determined. Results: From the flow cytometry, the expression of the CD146+ on the T cells includes 4.60 % in healthy patients, 10.10, 12.20, 9.80 % in adenocarcinoma, squamous and small cell lung cancer. The apoptosis of granulocytes, monocytes, lymphocytes, CD4+ and Treg were determined by 7-amino-actinomycin D/Annexin V-APC staining during the resting stage and after 24 h which indicate that apoptosis also occurred on Treg. Immunofluorescence shows the presence of CD146 in lung cancer patient's tissues. The methanol extracts of Calotropis procera leaf have antioxidant and anti-proliferative effects. Methanol extract of Calotropis procera leaf reduced CD146 expression on blood cells at 24 h. Conclusion: Increased CD146 expression in the Treg of lung cancer patients indicates that it may be a possible target for the treatment of lung cancer by utilizing potent immunotherapy or natural products such as methanol extract of Calotropis procera leaf which may ameliorate the expression of CD146. Calotropis procera has antioxidant, inhibitory capacity on H1299 lung cancer cells, and the ability to scavenge OH, H2O2, and NO radicals. Hence, this investigation strengthens the phyto-medicinal properties of Calotropis procera. [ABSTRACT FROM AUTHOR]