1. Single cell HaloChip assay on paper for point-of-care diagnosis
- Author
-
Ross D. Jones, Yong Qiao, Ming Su, Liyuan Ma, and Narendra P. Singh
- Subjects
Paper ,Lysis ,Materials science ,DNA damage ,Point-of-Care Systems ,Nanotechnology ,02 engineering and technology ,DNA Fragmentation ,Radiation ,Diamines ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Cell Line ,chemistry.chemical_compound ,Cell Line, Tumor ,Lab-On-A-Chip Devices ,Humans ,Benzothiazoles ,Organic Chemicals ,Fluorescent Dyes ,Staining and Labeling ,Cytotoxins ,010401 analytical chemistry ,Penetration (firestop) ,DNA ,Fibroblasts ,021001 nanoscience & nanotechnology ,Fluorescence ,Polyelectrolyte ,0104 chemical sciences ,chemistry ,Gamma Rays ,Biophysics ,MCF-7 Cells ,Quinolines ,Agarose ,Comet Assay ,0210 nano-technology ,Neuroglia - Abstract
This article describes a paper-based low cost single cell HaloChip assay that can be used to assess drug- and radiation-induced DNA damage at point-of-care. Printing ink on paper effectively blocks fluorescence of paper materials, provides high affinity to charged polyelectrolytes, and prevents penetration of water in paper. After exposure to drug or ionizing radiation, cells are patterned on paper to create discrete and ordered single cell arrays, embedded inside an agarose gel, lysed with alkaline solution to allow damaged DNA fragments to diffuse out of nucleus cores, and form diffusing halos in the gel matrix. After staining DNA with a fluorescent dye, characteristic halos formed around cells, and the level of DNA damage can be quantified by determining sizes of halos and nucleus with an image processing program based on MATLAB. With its low fabrication cost and easy operation, this HaloChip on paper platform will be attractive to rapidly and accurately determine DNA damage for point-of-care evaluation of drug efficacy and radiation condition. Graphical Abstract Single cell HaloChip on paper.
- Published
- 2016