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本研究旨在分离大熊猫肠道内唾液乳杆菌，为获得抑菌效果好，适应性强的熊猫源乳酸菌。通过选取健康大熊猫新鲜粪便，利用MRS培养基进行厌氧培养和单克隆纯化，通过形态学、生理生化鉴定、16S rDNA基因序列测定及同源性分析筛选到10株唾液乳杆菌。采用牛津杯法、活菌计数法、药敏纸片法（K B法）和PCR技术对分离菌株作进一步的耐受性、抑菌性和耐药性分析。结果表明：ZM02、ZZ03菌株在pH2.0的条件下培养2 h有76.62%、8075%的存活率；在胆盐浓度为0.3%的条件下培养2 h有97.76%、99%的存活率。抑菌试验结果表明：大肠杆菌抑菌效果最佳为ZM01（10.45±0.43）mm；乙型副伤寒沙门氏菌抑菌效果最佳为ZZ03（12.58±0.24）mm；铜绿假单胞菌抑菌效果最佳为ZZ04（13.99±0.29）mm；金黄色葡萄球菌抑菌效果最佳为ZM06（16.85±0.32）mm。耐药基因检测结果表明26对耐药基因仅有ZM02菌株未检测含有耐药基因。药敏实验表明：分离菌株对青霉素类（盘尼西林除外）、其他抗生素类（呋喃妥因、亚胺培南）和氯霉素类均无耐药。综上所述，本研究从大熊猫新鲜粪便中分离鉴定得到10株唾液乳酸杆菌并从中筛选到一株生物特性好、抑菌效果明显、不含耐药基因的唾液乳杆菌-编号103并命名ZM02。该研究可为后续熊猫源微生态制剂提供发展空间。

【目的】探讨活化态肝星状细胞（HSC）在柔软细胞外基质（ECM）中重新恢复为静息态的可能性。 【方法】稳定表达肌成纤维细胞（MFB）表型的HSC-T6培养于由1 mg/mL纤维蛋白水凝胶构建的二维（2D）或三 维（3D）体外培养基质模型之中，利用细胞活力检测试剂盒（CCK8）测定各时间段（24 h、48 h、72 h、96 h）的细胞 增殖活力，免疫荧光及明胶酶谱法检测细胞α平滑肌肌动蛋白（α-SMA）、基质金属蛋白酶-2（MMP-2）及MMP-9 的表达，原子力显微镜（AFM）探测细胞表面形貌及其变形能力。【结果】经纤维蛋白水凝胶培养后，活化态HSC- T6的细胞增殖活力、α-SMA、MMP-2和MMP-9的表达以及细胞变形能力均发生明显下调（P

[目的]运用伽玛刀和射频热凝技术毁损癌症顽痛患者的丘脑中央中核（CM核）,初步探讨它们治疗顽固性癌痛患者的镇痛效果和并发症。[方法]伽玛刀组共15例,并与我们已报道的射频热凝（共90例）治疗相比较。采用世界卫生组织推荐的10分法评定手术前后的疼痛程度。[结果]两组患者术前疼痛计分均在7分以上,术后降至0～3分。伽玛刀组随访发现,4例（27%）患者有不同程度疼痛复发,术后仅并发轻度的头痛、头昏和呕吐。既往发表的射频热凝组随访,有24例（27%）患者有不同程度疼痛复发,两组计分均在5分以下;其余患者均持续止痛,有3例（3%）达2年多。术后并发症：嗜睡15例,尿失禁10例,语言错乱8例,一侧动眼神经麻痹3例。经对症处理后两组的并发症均消失。[结论]CM核毁损是治疗各类顽固性癌痛有效的方法,伽玛刀和热凝射频的效果大体相同,伽玛刀比射频热凝毁损的并发症相对较轻和少。

太平鸟Bombycilla garrulus为旅顺地区的冬候鸟,通过对其越冬生态观察发现,此鸟是隔年迁来旅顺越冬,并且活动范围集中,初步分析认为这种现象是受太平鸟主要食物—龙柏树种子隔年成熟有直接关系。

黑尾鸥（Larus crassirostris）是蛇岛自然保护区主要繁殖鸟之一。在蛇岛,黑尾鸥从4月中旬开始筑巢,5月初开始产卵,每巢产卵1至3枚,以2枚的居多,5月下旬开始出雏,育雏期约为一个月。本文对黑尾鸥在繁殖期的活动习性,孵卵,育雏过程,雏鸟生长发育做了观察及测量统计,据结果作了初步总结。

准分子激光照射相位掩模后，在近场形成周期的条纹分布，利用近场光强对光敏光纤进行曝光，可以在纤芯中写入光栅。从衍射的频谱理论出发，导出具有一定发散角的准单色准分子激光照射掩模时产生的近场光强分布。分析了光源发散角及掩模衍射特性对纤芯近场光强分布及光纤光栅制作的影响。并把分析结果与实验现象进行了比较。

本文就光缆工程中遇到的外护套伤痕不明显，但光纤已断裂的情况进行了讨论。用光纤进行了模拟实验，给出了拉伸，压坏，折断等２６张较典型的断口端面的显微照片。对实际判断断裂原因提供一些有用的信息。

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Myeloid-derived suppressor cells (MDSCs) are a group of cells with potent immune suppressive activity which have emerged as an important contributor to tumor progression and genesis. More and more clinical researches have found that accumulation of MDSCs in peripheral blood and tumor tissues could predict dismal outcome for patients with malignancies, which was a potential prognostic tumor marker. In addition, immunosuppression inducedMDSCs could reduce the efficacy of anti-tumor therapies. Therefore, better understanding the mechanism of MDSCs' immune suppression in tumor microenvironment can provide reference for exploring new immunotherapies. [ABSTRACT FROM AUTHOR]

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为了对红花籽油产品开发提供参考,通过对相关既往研究的归纳总结，综述了红花籽油的主要营养成分、健康功效研究情况，介绍了红花籽油在其他领域的应用，并对未来的研究方向进行展望。红花籽油的营养功效成分主要有亚油酸、维生素E、植物甾醇、黄酮类化合物（主要为金合欢素）。红花籽油具有抗氧化、改善心脑血管健康、保护神经系统、改善肥胖等多种健康功效。红花籽油除食用外，在畜牧业中也具有一定的应用前景。今后应加大对红花籽油健康功效和作用机制的研究,创新红花籽油加工工艺,提高功效成分的保留率,促进红花籽油在多领域的应用。In order to provide reference for the development of safflower seed oil products, through the summary of relevant previous research, the main nutrients and health effects of safflower seed oil were reviewed, the application of safflower seed oil in other fields was introduced, and the future research direction was prospected. The main nutritional components of safflower seed oil are linoleic acid, vitamin E, phytosterols, and flavonoids (acacetin). Safflower seed oil has many health effects such as anti-oxidation, improving cardiovascular and cerebrovascular health, protecting the nervous system, and improving obesity. Safflower seed oil also has a certain application prospect in animal husbandry. In the future, the research on the health effect and mechanism of action of safflower seed oil should be increased, the processing process of safflower seed oil could be innovated, and the retention rate of effective ingredients should be improved so as to promote the application of safflower seed oil in many fields.

Objective To evaluate the implementation effects of Small Private Online Course(SPOC)-based online-offline mixed teaching mode in Traditional Chinese Medicine nursing teaching. Methods Totally 593 students from Grade 2019 and Grade 2020 of the School of Nursing of Beijing University of Chinese Medicine were taught with online-offline mixed teaching mode, the scores of whom were then compared with the scores of 371 students from Grade 2017 and Grade 2018 of the school of Nursing. In addition, interview was conducted to several students who were taught with online-offline mixed teaching mode, which means the effects of online-offline mixed teaching mode were evaluated from both subjective and objective perspectives. Results Before and after the implementation of the online-offline mixed teaching mode, there were significant differences in students' total scores (P0. 05). Students believed that the online-offline mixed teaching model effectively promoted their independent learning capability, but lacked effective interaction between teachers and students. Conclusion SPOC-based online-offline mixed teaching model can effectively improve students' subjective initiative in learning and more objectively reflect the effects of students' process learning. However, there are still some problems in the implementation of this teaching model, such as teachers and students being unable to communicate in time and relying too much on students' autonomy, which need to be further improved. (目的 评价基于SPOC的线上-线下混合式教学模式的实施效果。方法 对北京中医药大学护理学院2019级和2020级二本层次593名学生实施线上-线下混合式教学, 将学生成绩与教学改革实施前的2017级和2018级二本层次371名学生的成绩进行比较分析, 并对教学改革实施后的部分学生进行访谈, 从主客观两个角度评价线上-线下混合式教学模式的效果。结果 线上-线下混合式教学模式实施前后, 学生总成绩(P＜0. 01)、形成性考核成绩(P＜0. 05)及操作考核成绩(P＜0. 01)差异有统计学意义, 终末考核成绩、平时作业成绩及实验报告成绩均无显著差异(P＞0. 05)。学生认为, 线上-线下混合式教学模式有效推动了他们自主学习, 但缺乏师生有效互动。结论 基于SPOC的线上-线下混合式教学模式, 能够有效提升学生学习的主观能动性, 并能够更加客观地反映学生的过程性学习效果, 但教学模式实施过程中仍存在师生无法及时沟通、过于依赖学生自主性等问题, 有待进一步改善。)