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New 1,4-disubstituted &beta, pyrrolidino-1,2,3-triazoles were synthesized using a reusable copper-iodide-doped neutral alumina catalyst. Synthesis of diversely substituted triazoles and recyclability of CuI catalyst explains the broad scope of this protocol. The synthesized compounds were screened for their antimicrobial and anticancer properties. Most of the compounds showed significant antimicrobial activities against all the tested microorganisms compared to standard drugs. Furthermore, compounds 5a, 5e, 5g, 5h, 5i, and 5j showed moderate to potent activities against A549 and HepG-2 cells. In addition, compounds 5g and 5h displayed potential cytotoxicity activity against A549 cells with IC50 values of 72 &plusmn, 3.21 and 58 &plusmn, 2.31 &micro, M, respectively. The molecular docking study revealed that some of the synthesized compounds exhibited comparable binding as co-crystalized ligands with the DNA topoisomerase IV and anaplastic lymphoma kinase receptors.

Novel 1,4-disubstituted 1,2,3-triazolo bosentan derivatives 1a–n from bosentan 2 were synthesized in good yields by sequential chlorination, azidation followed by Cu(I) catalyzed 1,3-dipolar cycloaddition. All obtained compounds 1a–n were evaluated for their antimicrobial and in vitro anticancer activities and by in silico docking studies. Among all tested compounds 1e,f and 1h–j show better antimicrobial activities against the tested bacteria and fungi. When subjected to anticancer testing, compounds 1g–j and 1n show significant activities against both A549 and SKOV-3 cell lines with IC50 values at 7.81 μg mL−1 and among them compound 1i exhibited very potent activity. In addition, no toxicity was calculated up to 2 mg mL−1 in Vero cells. In silico studies were conducted to investigate the possible bonding modes of 1a–n with target receptors namely DNA topoisomerase IV (4 EMV) and anaplastic lymphoma kinase (2XP2). Among them, compounds 1e and 1h show maximum binding energies with 4EMV and 2XP2 receptors, respectively which also exhibited good antimicrobial and potent anticancer activities.