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Furazolidone, a banned nitrofuran antibiotic, is still illegally used in aquaculture and livestock husbandry. A commercial Enzyme-linked Immunosorbent Assay (ELISA) from Europroxima, The Netherlands is described, and validated for the screening of 3-amino-2-oxazolidinone (AOZ), metabolite of furazolidone antibiotic in the muscle of shrimp. Research works was conducted at Veterinary Drug Residue Analysis Laboratory, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka-1349, between July 2018 and December 2019. Validation was done in accordance with the European Commission (EC) decision 2002/657/EC in terms of specificity, detection capability CCβ, selectivity, ruggedness, and stability. Homogenized sample was subjected to acid hydrolysis for releasing of tissue bound metabolite AOZ, and derivatized with o-nitrobenzaldehyde followed by ethyl acetate/n-hexane extraction and detection by ELISA reader. The detection capability CCβ of the method was lower or at 0.3 µg/kg accepting 0% false compliant result. Cut-off factor Fm and the positivity threshold value T was 0.716 and 0.982, respectively. Mean OD of blank sample B was1.225. The assay was valid because T was greater than Fm and smaller than B. The kit was stable even after ten months of storage at recommended temperature and showed good sensitivity. From the practical point of view, the kit studied has good reliability, and could be used in the routine application for the control of illegal use of the drug.

Mustard oil is popular edible oil in Bangladesh. It contains about more than fifty percent erucic acid which is toxic to human beings. This study evaluated the determination of biochemical properties (acid value, iodine value, saponification value and unsaponifiable matter %) of mustard oil, virgin coconut and sesame oil and toxicity determination of these oils through blood indices targeting of these oil by mixing with normal diet as a dietary supplementation. This study found acid value of mustard, sesame and virgin coconut oil was 0.92, 0.312, 0.52 respectively. Iodine value of mustard, sesame and virgin coconut oil were 110.2, 105.2, and 11 respectively. Saponification value of wild mustard, sesame and virgin coconut oil was 171.1, 187.2 and 240 respectively and unsaponifiable matter % of mustard, sesame and virgin coconut oil was 1.19, 0.31 and 0.92 respectively. Also in vivo experiment we divided wistar male rats were 4 groups such as normal diet 12 g/day/rat, mustard oil (MO) 0.6 g/day/rat, virgin coconut oil (VCO) 0.6g/day/rat and sesame oil (SO) 0.6 g/day/rat. In this study the vegetable oil causes the effect on average body weight gain, Food Efficiency Ratio (FER), and lipid profiles [Total cholesterol (Tch), HDL- Cholesterol LDL - Cholesterol, Triglyceride (TG)] and serum enzymes [SGOT (AST), SGPT (ALT)] of different groups of rat also been estimated. The study found that mustard oil significantly (p

In the present study we formulated a low cost complementary baby food and investigated its role to improve the nutritional status of the malnourished baby. One hundred gm of complementary baby food was prepared by blending of 40 gm cereal powder, 16 gm green gram powder, 14 gm sesame powder, 14 gm banana powder, 10 gm pumpkin powder and 6 gm sugar powder according to the proportion of balanced diet. The formulated complementary baby food contained 336.5 Kcal energy and 6.56% moisture, 1.76% crude fiber, 17.5% protein, 13.7% fat, 31.31% carbohydrate, 30.93% ash, 27.7 mg of vitamin C, 210 µg of vitamin E, 30.15 mg of iron, 45.32 mg of zinc, 470.3 mg of calcium, 73.6 mg of magnesium and 1100 µg β-carotene. Impact of formulated complementary food to improve nutritional status was observed by conducting a cross-sectional study on 27 suspected malnourished children living in low socioeconomic status. According to the anthropometric data 18 children were found to be malnourished. From the 18 children, 13 children were taken as Feeding Group and remaining 5 children taken as Control Group. We found that 30.76% children were severe malnourished, 46.15% children were moderate malnourished and 23.07% children were mild malnourished according to Gomez classification. Nutritional status of the feeding and control group was investigated during the three months study period. It was found that control group gradually lost their weight (wasting) during the study periods due to faulty feeding practices and lack of calorie intake. Percent weight loss (wasting) at the end of the study period for Control-1, Control-2, Control-3, Control-4 and Control-5 were 8.60%, 7.14%, 8.33%, 10.0%, and 9.25% respectively. While the newly formulated complementary baby food was provided 120gm/day for the severe malnourished children and 80gm/day for the mild to moderate malnourished children during the study period. It was found that the formulated complementary baby food can reverse muscle wasting and improving nutritional status of the malnourished children during the study period. Thus newly formulated complementary baby food was efficiently providing both calorie and other dietary essentials to the malnourished children.